BuceA.12800.g

LysR family regulatory protein

CENTER ID: BuceA.12800.g
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.g.A1.GE31862 Full length( BuceA.12800.g ) 1 311
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2787
RefSeq: YP_002231796.1
UniProt: B4E8W6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDKLDQVRIF LQVAEMGSFI KAAHALDAPR ATVSAAVQQL ETALGTRLLH RTTRQVQLTA DGALLLERGR RLLAEADELD RLFRRRDHDV VGRLNVDVPS RIARRVVAPA LPSLFRRYPK LQLSLGSTDR SIDLVQEGVD CAIRVGRLAD SSLVVRPLGQ FTLINCASPD YLRECGVPEH PDALAHGHWA IGYASPTTGR ELGWEYCADG QRRVLTLPSR VIVNNAETYI ASCIAGMGLI QIPRFDVEHL LASGALVDVM PGHRAEPMDV SAVYPHRRHR SRRLNAFIEW FGELMADALT GTGEAASSDG D
NT Sequence
ATGGACAAGC TGGATCAGGT CAGGATCTTC CTGCAGGTTG CCGAGATGGG CAGCTTCATC AAGGCCGCGC ATGCGCTCGA CGCGCCGCGC GCGACCGTGT CGGCGGCCGT CCAGCAACTG GAGACGGCGC TCGGCACGCG TCTGTTGCAC CGTACGACGC GCCAGGTGCA ATTGACTGCC GACGGCGCGC TGCTGCTGGA GCGCGGTCGG CGCCTGCTCG CGGAGGCCGA TGAACTCGAC CGCCTGTTTC GGCGCCGCGA CCACGACGTG GTCGGGCGGC TGAACGTCGA CGTGCCGAGC CGGATCGCGC GGCGCGTCGT GGCGCCCGCG CTGCCGTCGC TGTTTCGCCG CTATCCGAAG CTGCAGCTGT CGCTCGGCTC CACGGACCGC TCGATCGACC TCGTGCAGGA GGGCGTCGAT TGCGCGATTC GCGTCGGGCG GCTCGCGGAC AGCAGCCTCG TCGTGCGGCC GCTCGGGCAG TTCACGCTGA TCAACTGCGC GAGCCCCGAC TACCTGCGCG AGTGCGGCGT GCCCGAGCAT CCGGACGCGC TCGCGCACGG GCACTGGGCG ATCGGCTATG CGTCCCCGAC GACGGGGCGC GAACTCGGCT GGGAATACTG CGCGGACGGG CAGCGGCGCG TGCTGACGCT GCCGAGCCGC GTGATCGTCA ACAACGCCGA AACCTATATC GCCAGCTGCA TCGCGGGCAT GGGGCTGATC CAGATTCCGC GCTTCGACGT CGAGCATCTG CTGGCAAGCG GCGCGCTCGT CGACGTGATG CCCGGCCATC GCGCCGAGCC GATGGACGTG TCGGCCGTGT ATCCGCACCG CCGGCACCGG TCGAGGCGGC TCAATGCGTT CATCGAATGG TTCGGGGAAC TGATGGCCGA TGCGCTGACG GGGACCGGCG AAGCAGCGAG TTCGGACGGC GAC
Details for BuceA.12800.g.A1.GE31862
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 17
PERCENT COVERAGE: 97
Validated AA Sequence
MNAXXXLDRX RRXGYTADTS IGSARXXGHH VDERAACXXX LDVEARNLDQ PHAXXXAGDI GXGVVDDHAA RQRQHAPLPV RAVFPAEFAP RRRGRIADRP VPVRERVRML GHAALAQVVG ARAVDQRELP ERPHDEAAVR EPPDANRAID ALLHEVDRAV RGAERQLQLR IAAKQRRQRG RHDAPRDPAR HVDVQPPDHV VVAAPKQAVE FIGLREQAPT ALQQQRAVGS QLHLARRTVQ QTRAERRLQL LDGRRHGRAR RVERMRGLDE AAHLGNLQED PDLIQLVHRT RTLGLSFQGT HMMVMVXXXG XXXXXXXXXX XXXX
Validated NT Sequence
cnnnnnnnnn nnnnnnnnnn nnnngcgnnn nnnnnnnntg nntnccntgg ntcancacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tggacaagct ggatcaggtc aggatcttcc tgcaggttgc cgagatgggc agcttcatca aggccgcgca tgcgctcgac gcgccgcgcg cgaccgtgtc ggcggccgtc cagcaactgg agacggcgct cggcacgcgt ctgttgcacc gtacgacgcg ccaggtgcaa ttgactgccg acggcgcgct gctgctggag cgcggtcggc gcctgctcgc ggaggccgat gaactcgacc gcctgtttcg gcgccgcgac cacgacgtgg tcgggcggct gaacgtcgac gtgccgagcc ggatcgcgcg gcgcgtcgtg gcgcccgcgc tgccgtcgct gtttcgccgc tatccgaagc tgcagctgtc gctcggctcc acggaccgct cgatcgacct cgtgcaggag ggcgtcgatt gcgcgattcg cgtcgggcgg ctcgcggaca gcagcctcgt cgtgcggccg ctcgggcagt tcacgctgat caactgcgcg agccccgact acctgcgcga gtgcggcgtg cccgagcatc cggacgcgct cgcgcacggg cactgggcga tcggctatgc gtccccgacg acggggcgcg aactcggctg ggaatactgc gcggacgggc agcggcgcgt gctgacgctg ccgagccgcg tgatcgtcaa caacgccnaa acctatatcg ccagctgnnn nnngggcatg gggctgatcc agattccgcg cttcgacgtc gagcannnnn nggcaagcgg cgcgctcgtc gacgtgatgc ccggnncatc gcgccgagcc gatggacgtg tcngccgtgt atccncaccg ccggnaccgg tcgaggcngn tcantgcgtt catcnnatgg ttcggggaac tgatggnccg atgnnctgan gggnncnggc nnnncnncnn gtncngnngn gactaannaa anannacgna nnnngttctg cancnnngct nntnnnnngc tannnncnnn nnngnnnctn anntnngctn nnnnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDKLDQVRI FLQVAEMGSF IKAAHALDAP RATVSAAVQQ LETALGTRLL HRTTRQVQLT ADGALLLERG RRLLAEADEL DRLFRRRDHD VVGRLNVDVP SRIARRVVAP ALPSLFRRYP KLQLSLGSTD RSIDLVQEGV DCAIRVGRLA DSSLVVRPLG QFTLINCASP DYLRECGVPE HPDALAHGHW AIGYASPTTG RELGWEYCAD GQRRVLTLPS RVIVNNAETY IASCIAGMGL IQIPRFDVEH LLASGALVDV MPGHRAEPMD VSAVYPHRRH RSRRLNAFIE WFGELMADAL TGTGEAASSD GD
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagctg gatcaggtca ggatcttcct gcaggttgcc gagatgggca gcttcatcaa ggccgcgcat gcgctcgacg cgccgcgcgc gaccgtgtcg gcggccgtcc agcaactgga gacggcgctc ggcacgcgtc tgttgcaccg tacgacgcgc caggtgcaat tgactgccga cggcgcgctg ctgctggagc gcggtcggcg cctgctcgcg gaggccgatg aactcgaccg cctgtttcgg cgccgcgacc acgacgtggt cgggcggctg aacgtcgacg tgccgagccg gatcgcgcgg cgcgtcgtgg cgcccgcgct gccgtcgctg tttcgccgct atccgaagct gcagctgtcg ctcggctcca cggaccgctc gatcgacctc gtgcaggagg gcgtcgattg cgcgattcgc gtcgggcggc tcgcggacag cagcctcgtc gtgcggccgc tcgggcagtt cacgctgatc aactgcgcga gccccgacta cctgcgcgag tgcggcgtgc ccgagcatcc ggacgcgctc gcgcacgggc actgggcgat cggctatgcg tccccgacga cggggcgcga actcggctgg gaatactgcg cggacgggca gcggcgcgtg ctgacgctgc cgagccgcgt gatcgtcaac aacgccgaaa cctatatcgc cagctgcatc gcgggcatgg ggctgatcca gattccgcgc ttcgacgtcg agcatctgct ggcaagcggc gcgctcgtcg acgtgatgcc cggccatcgc gccgagccga tggacgtgtc ggccgtgtat ccgcaccgcc ggcaccggtc gaggcggctc aatgcgttca tcgaatggtt cggggaactg atggccgatg cgctgacggg gaccggcgaa gcagcgagtt cggacggcga ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa