BuceA.12800.e

LysR family regulatory protein

CENTER ID: BuceA.12800.e
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.e.A2.GE31860 Mature protein( BuceA.12800.e ) 22 308
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2646
RefSeq: YP_002231658.1
UniProt: B4E7N6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDRLLSMAVF VSAVEEGSLV SAARRFGLSP SMAGKHVSAI EEQLKVRLLQ RSTRKLTLTD VGRSYYTRCK RLLEEYEDAN REAQDAQQSV HGILRVAAPT TFGALHLGRV VSSYLAEYPD VSIETTLSDR YVDLLADGID VAIRIGRLQD SDLVARRLAS CRMMFCAAPS FLARHGEPKT VEQLRQAPRL AFSQAVSAGD WSVTDANGQT HHIDGAIRLA ANNMQMLLAA ALTGAGVVYG PSFVFEQSIA SGALRVLLPD HKTTDLAIHA IYPSKRHVSL KVRRFIEHLS MSFGDMQPWD LMHRSTTA
NT Sequence
ATGGATAGAT TGCTCAGCAT GGCGGTGTTC GTCTCCGCAG TCGAAGAAGG TAGCTTGGTA AGCGCCGCTC GACGGTTTGG CCTGTCACCC TCGATGGCGG GCAAGCACGT TTCCGCGATC GAGGAGCAGT TGAAAGTTCG GTTGCTTCAA CGCAGTACGC GCAAGTTGAC GCTAACGGAT GTAGGAAGGT CCTACTACAC GCGATGCAAG CGCTTACTGG AAGAATACGA GGACGCGAAC CGCGAAGCCC AGGACGCGCA ACAGAGTGTG CATGGTATTT TGCGCGTGGC TGCGCCGACA ACCTTTGGGG CACTGCATCT TGGTCGTGTT GTATCGAGTT ATCTGGCTGA ATACCCCGAT GTCTCGATCG AGACCACGCT GAGCGATCGC TATGTCGATC TACTGGCAGA TGGCATCGAC GTTGCTATCC GCATCGGCCG TCTGCAAGAC TCCGACCTCG TTGCTCGGCG GCTCGCCTCG TGTCGCATGA TGTTCTGCGC AGCGCCATCT TTTTTGGCAC GACATGGCGA GCCGAAAACC GTCGAACAGC TCCGCCAGGC ACCTCGGCTC GCTTTCTCCC AGGCAGTTTC AGCCGGAGAC TGGAGCGTCA CGGATGCTAA CGGCCAGACC CATCACATTG ATGGTGCGAT CCGGCTCGCT GCAAACAACA TGCAGATGCT GCTAGCGGCA GCGCTGACAG GGGCGGGAGT GGTCTACGGC CCCAGCTTCG TGTTCGAGCA GTCCATCGCC AGCGGTGCGC TTCGTGTCCT GCTGCCCGAC CACAAAACAA CTGATTTGGC GATTCATGCC ATCTATCCGA GCAAGCGGCA TGTGTCGTTG AAAGTTCGCC GCTTCATCGA GCATCTGAGC ATGAGCTTCG GCGACATGCA GCCATGGGAT CTCATGCATA GGAGCACTAC GGCT
Details for BuceA.12800.e.A2.GE31860
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 34kDa
OBSERVED MW: 33kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 91
PERCENT COVERAGE: 109
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAARRFGLS PSMAGKHVSA IEEQLKVRLL QRSTRKLTLT DVGRSYYTRC KRLLEEYEDA NREAQDAQQS VHGILRVAAP TTFGALHLGR VVSSYLAEYP DVSIETTLSD RYVDLLADGI DVAIRIGRLQ DSDLVARRLA SCRMMFCAAP SFLARHGEPK TVEQLRQAPR LAFSQAVSAG DWSVTDANGQ THHIDGAIRL AAXNMQMLLA AALTGAGVVY GPSFVFEQSI ASGALRVLLP DHKTTDLAIH AIYPXXAACV VESSPLHRAS EHELXRHAPW DLMHRSTXXX KQHXQXLQXX XSXXXLLTXP KXAXXAAX
Validated NT Sequence
gnnnnnnnnn nnnnnnannn gantaccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggccgctc gacggtttgg cctgtcaccc tcgatggcgg gcaagcacgt ttccgcgatc gaggagcagt tgaaagttcg gttgcttcaa cgcagtacgc gcaagttgac gctaacggat gtaggaaggt cctactacac gcgatgcaag cgcttactgg aagaatacga ggacgcgaac cgcgaagccc aggacgcgca acagagtgtg catggtattt tgcgcgtggc tgcgccgaca acctttgggg cactgcatct tggtcgtgtt gtatcgagtt atctggctga ataccccgat gtctcgatcg agaccacgct gagcgatcgc tatgtcgatc tactggcaga tggcatcgac gttgctatcc gcatcggccg tctgcaagac tccgacctcg ttgctcggcg gctcgcctcg tgtcgcatga tgttctgcgc agcgccatct tttttggcac gacatggcga gccgaaaacc gtcgaacagc tccgccaggc acctcggctc gctttctccc aggcagtttc agccggagac tggagcgtca cggatgctaa cggccagacc catcacattg atggtgcgat ccggctcgct gcnnacaaca tgcagatgct gctagcggca gcgctgacag gngcgggagt ggtctacggc cccagcttcg tgttcgagca gtccatcgcc agcggtgcgc ttcgtgtcct gctgcccgac cacaaaacaa ctgatttggc gattcatgcc atctatccnn nnnnngcggc atgtgtcgtt gaaagttcgc cgcttcatcg agcatctgag catgagcttc ngcgacatgc accntgggat ctcatgcata ggagcactan nnctnnnaaa cagcacgnac aannnctgca gncnngctnc tcnannntnn nnctgctnac aaanccgaaa gnagctgann nngctgctnn nnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAARRFGLS PSMAGKHVSA IEEQLKVRLL QRSTRKLTLT DVGRSYYTRC KRLLEEYEDA NREAQDAQQS VHGILRVAAP TTFGALHLGR VVSSYLAEYP DVSIETTLSD RYVDLLADGI DVAIRIGRLQ DSDLVARRLA SCRMMFCAAP SFLARHGEPK TVEQLRQAPR LAFSQAVSAG DWSVTDANGQ THHIDGAIRL AANNMQMLLA AALTGAGVVY GPSFVFEQSI ASGALRVLLP DHKTTDLAIH AIYPSKRHVS LKVRRFIEHL SMSFGDMQPW DLMHRSTTA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggccgctcga cggtttggcc tgtcaccctc gatggcgggc aagcacgttt ccgcgatcga ggagcagttg aaagttcggt tgcttcaacg cagtacgcgc aagttgacgc taacggatgt aggaaggtcc tactacacgc gatgcaagcg cttactggaa gaatacgagg acgcgaaccg cgaagcccag gacgcgcaac agagtgtgca tggtattttg cgcgtggctg cgccgacaac ctttggggca ctgcatcttg gtcgtgttgt atcgagttat ctggctgaat accccgatgt ctcgatcgag accacgctga gcgatcgcta tgtcgatcta ctggcagatg gcatcgacgt tgctatccgc atcggccgtc tgcaagactc cgacctcgtt gctcggcggc tcgcctcgtg tcgcatgatg ttctgcgcag cgccatcttt tttggcacga catggcgagc cgaaaaccgt cgaacagctc cgccaggcac ctcggctcgc tttctcccag gcagtttcag ccggagactg gagcgtcacg gatgctaacg gccagaccca tcacattgat ggtgcgatcc ggctcgctgc aaacaacatg cagatgctgc tagcggcagc gctgacaggg gcgggagtgg tctacggccc cagcttcgtg ttcgagcagt ccatcgccag cggtgcgctt cgtgtcctgc tgcccgacca caaaacaact gatttggcga ttcatgccat ctatccgagc aagcggcatg tgtcgttgaa agttcgccgc ttcatcgagc atctgagcat gagcttcggc gacatgcagc catgggatct catgcatagg agcactacgg cttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa