BuceA.12800.c

LysR family regulatory protein

CENTER ID: BuceA.12800.c
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12800.c.A1.GE31858 Full length( BuceA.12800.c ) 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1514
RefSeq: YP_002230588.1
UniProt: B4E723

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDTLQNMRVF VRVVDAGSFT AAAQQMNSTT AYASRAVSDL EAHLRTRLLN RTTRRIALTE AGERYLQRCE QILAYVDQAE AEAGDAHARP SGKLKVHCFT SLGQHYLVPA IARYRERYPD VHVELTLAQR MPDLLDEGYD VAIVVGRDLP DSGLVSQRLG ESYSVVCASP GYVESHGVPQ RPADLAQHVC LGMVAPGFHF DEWALAGPNG DEVVPITAPP FRVNVAEALA VAVREGMGVG GLPLYSAIGW LRSGHIVRVM PEYRSHVMNI YALYPSRQYL DAKIRTWVDF LREELPLTLE ADEAALEQYT RAT
NT Sequence
ATGGATACGT TACAAAACAT GCGGGTATTC GTCCGCGTGG TCGACGCGGG CAGCTTTACC GCGGCCGCCC AGCAGATGAA TTCGACCACC GCCTACGCGT CGCGCGCGGT CTCGGATCTC GAGGCCCACC TGCGCACGCG TCTCCTGAAC CGCACGACGC GCCGGATCGC GCTGACCGAG GCCGGCGAGC GTTATCTGCA GCGCTGCGAA CAGATCCTCG CTTACGTCGA CCAGGCCGAA GCCGAGGCGG GCGACGCGCA CGCGCGCCCG TCCGGCAAGC TGAAGGTCCA TTGCTTCACG AGCCTCGGCC AGCACTATCT GGTGCCGGCC ATCGCGCGCT ACCGCGAGCG CTATCCGGAC GTGCACGTCG AGCTGACGCT CGCGCAGCGG ATGCCCGACC TGCTCGACGA GGGGTACGAC GTCGCGATCG TCGTCGGCCG CGACCTGCCC GATTCGGGGC TCGTGTCGCA GCGGCTCGGC GAGAGCTACA GCGTCGTCTG CGCATCGCCG GGCTACGTCG AGTCGCACGG CGTGCCGCAG CGGCCGGCCG ATCTCGCACA GCACGTGTGC CTGGGGATGG TCGCGCCGGG CTTTCACTTC GACGAATGGG CGCTGGCGGG GCCGAACGGC GACGAGGTCG TGCCGATCAC GGCGCCGCCG TTTCGCGTGA ACGTCGCCGA GGCGCTCGCG GTGGCCGTGC GGGAGGGGAT GGGCGTCGGC GGGCTGCCGC TCTATTCGGC GATCGGCTGG CTGCGTAGCG GGCACATCGT GCGCGTGATG CCCGAGTACC GGTCGCACGT GATGAACATC TACGCGCTGT ATCCGTCGCG CCAGTACCTC GACGCGAAAA TCCGCACCTG GGTCGATTTC CTGCGCGAAG AGCTGCCGCT CACGCTCGAA GCGGACGAAG CGGCGCTCGA GCAGTACACG CGTGCGACA
Details for BuceA.12800.c.A1.GE31858
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 36kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 97
Validated AA Sequence
MGTLEAQTQG PGSMDTLQNM RVFVRVVDAG SFTAAAQQMN STTAYASRAV SDLEAHLRTR LLNRTTRRIA LTEAGERYLQ RCEQILAYVD QAEAEAGDAH ARPSGKLKVH CFTSLGQHYL VPAIARYRER YPDVHVELTL AQRMPDLLDE GYDVAIVVGR DLPDSGLVSQ RLGESYSVVC ASPGYVESHG VPQRPADLAQ HVCLGMVAPG FHFDEWALAG PNGDEVVPIT APPFRVNVAE ALAVAVREGM GVGGLPLYSA IGWLRSGHIV RVMPEYRSHV MNIYALYPSR QYLDAKIRTW VDFLREELPL TLEADEAALE QYTR
Validated NT Sequence
cacgcgtgta ctgctcgagc gccgcttcgt ccgcttcgag cgtgagcggc agctcttcgc gcaggaaatc gacccaggtg cggattttcg cgtcgaggta ctggcgcgac ggatacagcg cgtagatgtt catcacgtgc gaccggtact cgggcatcac gcgcacgatg tgcccgctac gcagccagcc gatcgccgaa tagagcggca gcccgccgac gcccatcccc tcccgcacgg ccaccgcgag cgcctcggcg acgttcacgc gaaacggcgg cgccgtgatc ggcacgacct cgtcgccgtt cggccccgcc agcgcccatt cgtcgaagtg aaagcccggc gcgaccatcc ccaggcacac gtgctgtgcg agatcggccg gccgctgcgg cacgccgtgc gactcgacgt agcccggcga tgcgcagacg acgctgtagc tctcgccgag ccgctgcgac acgagccccg aatcgggcag gtcgcggccg acgacgatcg cgacgtcgta cccctcgtcg agcaggtcgg gcatccgctg cgcgagcgtc agctcgacgt gcacgtccgg atagcgctcg cggtagcgcg cgatggccgg caccagatag tgctggccga ggctcgtgaa gcaatggacc ttcagcttgc cggacgggcg cgcgtgcgcg tcgcccgcct cggcttcggc ctggtcgacg taagcgagga tctgttcgca gcgctgcaga taacgctcgc cggcctcggt cagcgcgatc cggcgcgtcg tgcggttcag gagacgcgtg cgcaggtggg cctcgagatc cgagaccgcg cgcgacgcgt aggcggtggt cgaattcatc tgctgggcgg ccgcggtaaa gctgcccgcg tcgaccacgc ggacgaatac ccgcatgttt tgtaacgtat ccatcgaacc aggaccctgg gtctgagctt ccagggtacc catatgatgg tg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDTLQNMRV FVRVVDAGSF TAAAQQMNST TAYASRAVSD LEAHLRTRLL NRTTRRIALT EAGERYLQRC EQILAYVDQA EAEAGDAHAR PSGKLKVHCF TSLGQHYLVP AIARYRERYP DVHVELTLAQ RMPDLLDEGY DVAIVVGRDL PDSGLVSQRL GESYSVVCAS PGYVESHGVP QRPADLAQHV CLGMVAPGFH FDEWALAGPN GDEVVPITAP PFRVNVAEAL AVAVREGMGV GGLPLYSAIG WLRSGHIVRV MPEYRSHVMN IYALYPSRQY LDAKIRTWVD FLREELPLTL EADEAALEQY TRAT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatacgtta caaaacatgc gggtattcgt ccgcgtggtc gacgcgggca gctttaccgc ggccgcccag cagatgaatt cgaccaccgc ctacgcgtcg cgcgcggtct cggatctcga ggcccacctg cgcacgcgtc tcctgaaccg cacgacgcgc cggatcgcgc tgaccgaggc cggcgagcgt tatctgcagc gctgcgaaca gatcctcgct tacgtcgacc aggccgaagc cgaggcgggc gacgcgcacg cgcgcccgtc cggcaagctg aaggtccatt gcttcacgag cctcggccag cactatctgg tgccggccat cgcgcgctac cgcgagcgct atccggacgt gcacgtcgag ctgacgctcg cgcagcggat gcccgacctg ctcgacgagg ggtacgacgt cgcgatcgtc gtcggccgcg acctgcccga ttcggggctc gtgtcgcagc ggctcggcga gagctacagc gtcgtctgcg catcgccggg ctacgtcgag tcgcacggcg tgccgcagcg gccggccgat ctcgcacagc acgtgtgcct ggggatggtc gcgccgggct ttcacttcga cgaatgggcg ctggcggggc cgaacggcga cgaggtcgtg ccgatcacgg cgccgccgtt tcgcgtgaac gtcgccgagg cgctcgcggt ggccgtgcgg gaggggatgg gcgtcggcgg gctgccgctc tattcggcga tcggctggct gcgtagcggg cacatcgtgc gcgtgatgcc cgagtaccgg tcgcacgtga tgaacatcta cgcgctgtat ccgtcgcgcc agtacctcga cgcgaaaatc cgcacctggg tcgatttcct gcgcgaagag ctgccgctca cgctcgaagc ggacgaagcg gcgctcgagc agtacacgcg tgcgacataa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa