BuceA.12800.b

LysR family regulatory protein

CENTER ID: BuceA.12800.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BuceA.12800.b.A1.GE31857 Full length( BuceA.12800.b ) 1 307
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
BuceA.12800.b.A1.PS01335 Full length( BuceA.12800.b ) 1 307

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1456
RefSeq: YP_002230532.1
UniProt: B4E6C3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQHLPDLEAW AIFARVAETG SFAKAADLLG VSQPTVSKAI ARLEKRLGAM LLYRTSRKLS LTPTGELLRD RAIRLLADAE LIENEASAQA LEPHGLVRVN APMSFGLRHL SPVLPAFLER YPRVDIDLVL TDHIVDIVSG GFDVAIRIAE LNDSTLRSRR LCAVRRPLVA SPAYLDRRGR PTHPRDIEQH VCLTYTNLPT PEHWRFRHPA SGDEVGVSVH GRIRTNNADV ILPALVGGHG LALQPEFLVW DAMQRGELEE VMSDWKIADI NVNLVTPPGM LRAARVTVLL DFLAERFAHA PWALPAA
NT Sequence
ATGCAACACC TTCCTGATCT CGAAGCCTGG GCCATCTTTG CGCGCGTCGC GGAGACCGGC TCGTTCGCGA AAGCCGCCGA CCTGCTCGGC GTATCGCAAC CCACCGTGTC GAAGGCGATC GCGCGCCTCG AAAAACGCCT CGGCGCGATG CTGCTGTACC GCACGTCGCG CAAGCTGTCG CTCACGCCGA CCGGCGAACT GCTGCGCGAC CGCGCGATCC GCCTGCTCGC CGATGCGGAG CTGATCGAGA ACGAAGCGTC CGCGCAGGCG CTCGAGCCGC ACGGCCTCGT GCGCGTGAAT GCGCCGATGT CGTTCGGGCT GCGCCATCTG TCGCCGGTGC TGCCGGCGTT TCTCGAACGC TATCCGCGCG TCGACATCGA TCTCGTGCTC ACCGATCACA TCGTCGACAT CGTGTCGGGC GGCTTCGACG TCGCGATCCG CATCGCCGAG CTGAACGATT CGACGCTGCG TTCGCGCCGC CTCTGCGCGG TGCGCCGGCC GCTCGTCGCA TCGCCCGCGT ATCTCGACCG GCGCGGCCGC CCCACGCATC CGCGCGACAT CGAACAGCAC GTGTGCCTCA CGTACACCAA CTTGCCGACC CCCGAGCACT GGCGCTTCCG CCATCCGGCG TCGGGCGACG AAGTCGGCGT GTCCGTCCAC GGGCGCATCC GCACCAACAA CGCGGACGTG ATCCTCCCCG CGCTCGTCGG CGGTCACGGC CTGGCGCTGC AGCCCGAGTT CCTCGTGTGG GACGCGATGC AGCGCGGCGA GCTCGAGGAA GTGATGAGCG ACTGGAAGAT CGCGGACATC AACGTGAACC TCGTCACGCC GCCCGGCATG CTGCGCGCCG CGCGCGTGAC GGTGCTGCTC GACTTCCTCG CGGAACGCTT CGCGCACGCG CCGTGGGCGC TGCCCGCCGC C
Details for BuceA.12800.b.A1.GE31857
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 36kDa
OBSERVED MW: 37kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MQHLPDLEAW AIFARVAETG SFAKAADLLG VSQPTVSKAI ARLEKRLGAM LLYRTSRKLS LTPTGELLRD RAIRLLADAE LIENEASAQA LEPHGLVRVN APMSFGLRHL SPVLPAFLER YPRVDIDLVL TDHIVDIVSG GFDVAIRIAE LNDSTLRSRR LCAVRRPLVA SPAYLDRRGR PTHPRDIEQH VCLTYTNLPT PEHWRFRHPA SGDEVGVSVH GRIRTNNADV ILPALVGGHG LALQPEFLVW DAMQRGELEE VMSDWKIADI NVNLVTPPGM LRAARVTVLL DFLAERFAHA PWALPAA
Validated NT Sequence
ttattaggcg gcgggcagcg cccacggcgc gtgcgcgaag cgttccgcga ggaagtcgag cagcaccgtc acgcgcgcgg cgcgcagcat gccgggcggc gtgacgaggt tcacgttgat gtccgcgatc ttccagtcgc tcatcacttc ctcgagctcg ccgcgctgca tcgcgtccca cacgaggaac tcgggctgca gcgccaggcc gtgaccgccg acgagcgcgg ggaggatcac gtccgcgttg ttggtgcgga tgcgcccgtg gacggacacg ccgacttcgt cgcccgacgc cggatggcgg aagcgccagt gctcgggggt cggcaagttg gtgtacgtga ggcacacgtg ctgttcgatg tcgcgcggat gcgtggggcg gccgcgccgg tcgagatacg cgggcgatgc gacgagcggc cggcgcaccg cgcagaggcg gcgcgaacgc agcgtcgaat cgttcagctc ggcgatgcgg atcgcgacgt cgaagccgcc cgacacgatg tcgacgatgt gatcggtgag cacgagatcg atgtcgacgc gcggatagcg ttcgagaaac gccggcagca ccggcgacag atggcgcagc ccgaacgaca tcggcgcatt cacgcgcacg aggccgtgcg gctcgagcgc ctgcgcggac gcttcgttct cgatcagctc cgcatcggcg agcaggcgga tcgcgcggtc gcgcagcagt tcgccggtcg gcgtgagcga cagcttgcgc gacgtgcggt acagcagcat cgcgccgagg cgtttttcga ggcgcgcgat cgccttcgac acggtgggtt gcgatacgcc gagcaggtcg gcggctttcg cgaacgagcc ggtctccgcg acgcgcgcaa agatggccca ggcttcgaga tcaggaaggt gttgcatcga accaggaccc tgggtctgag cttccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQHLPDLEA WAIFARVAET GSFAKAADLL GVSQPTVSKA IARLEKRLGA MLLYRTSRKL SLTPTGELLR DRAIRLLADA ELIENEASAQ ALEPHGLVRV NAPMSFGLRH LSPVLPAFLE RYPRVDIDLV LTDHIVDIVS GGFDVAIRIA ELNDSTLRSR RLCAVRRPLV ASPAYLDRRG RPTHPRDIEQ HVCLTYTNLP TPEHWRFRHP ASGDEVGVSV HGRIRTNNAD VILPALVGGH GLALQPEFLV WDAMQRGELE EVMSDWKIAD INVNLVTPPG MLRAARVTVL LDFLAERFAH APWALPAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaacacctt cctgatctcg aagcctgggc catctttgcg cgcgtcgcgg agaccggctc gttcgcgaaa gccgccgacc tgctcggcgt atcgcaaccc accgtgtcga aggcgatcgc gcgcctcgaa aaacgcctcg gcgcgatgct gctgtaccgc acgtcgcgca agctgtcgct cacgccgacc ggcgaactgc tgcgcgaccg cgcgatccgc ctgctcgccg atgcggagct gatcgagaac gaagcgtccg cgcaggcgct cgagccgcac ggcctcgtgc gcgtgaatgc gccgatgtcg ttcgggctgc gccatctgtc gccggtgctg ccggcgtttc tcgaacgcta tccgcgcgtc gacatcgatc tcgtgctcac cgatcacatc gtcgacatcg tgtcgggcgg cttcgacgtc gcgatccgca tcgccgagct gaacgattcg acgctgcgtt cgcgccgcct ctgcgcggtg cgccggccgc tcgtcgcatc gcccgcgtat ctcgaccggc gcggccgccc cacgcatccg cgcgacatcg aacagcacgt gtgcctcacg tacaccaact tgccgacccc cgagcactgg cgcttccgcc atccggcgtc gggcgacgaa gtcggcgtgt ccgtccacgg gcgcatccgc accaacaacg cggacgtgat cctccccgcg ctcgtcggcg gtcacggcct ggcgctgcag cccgagttcc tcgtgtggga cgcgatgcag cgcggcgagc tcgaggaagt gatgagcgac tggaagatcg cggacatcaa cgtgaacctc gtcacgccgc ccggcatgct gcgcgccgcg cgcgtgacgg tgctgctcga cttcctcgcg gaacgcttcg cgcacgcgcc gtgggcgctg cccgccgcct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BuceA.12800.b.A1.PS01335
PURIFICATION DATe: 1/11/2012
CONCENTRATION: 5.26mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Protocol Notes
notes unavailable
Validated AA Sequence
MQHLPDLEAW AIFARVAETG SFAKAADLLG VSQPTVSKAI ARLEKRLGAM LLYRTSRKLS LTPTGELLRD RAIRLLADAE LIENEASAQA LEPHGLVRVN APMSFGLRHL SPVLPAFLER YPRVDIDLVL TDHIVDIVSG GFDVAIRIAE LNDSTLRSRR LCAVRRPLVA SPAYLDRRGR PTHPRDIEQH VCLTYTNLPT PEHWRFRHPA SGDEVGVSVH GRIRTNNADV ILPALVGGHG LALQPEFLVW DAMQRGELEE VMSDWKIADI NVNLVTPPGM LRAARVTVLL DFLAERFAHA PWALPAA
Validated NT Sequence
ttattaggcg gcgggcagcg cccacggcgc gtgcgcgaag cgttccgcga ggaagtcgag cagcaccgtc acgcgcgcgg cgcgcagcat gccgggcggc gtgacgaggt tcacgttgat gtccgcgatc ttccagtcgc tcatcacttc ctcgagctcg ccgcgctgca tcgcgtccca cacgaggaac tcgggctgca gcgccaggcc gtgaccgccg acgagcgcgg ggaggatcac gtccgcgttg ttggtgcgga tgcgcccgtg gacggacacg ccgacttcgt cgcccgacgc cggatggcgg aagcgccagt gctcgggggt cggcaagttg gtgtacgtga ggcacacgtg ctgttcgatg tcgcgcggat gcgtggggcg gccgcgccgg tcgagatacg cgggcgatgc gacgagcggc cggcgcaccg cgcagaggcg gcgcgaacgc agcgtcgaat cgttcagctc ggcgatgcgg atcgcgacgt cgaagccgcc cgacacgatg tcgacgatgt gatcggtgag cacgagatcg atgtcgacgc gcggatagcg ttcgagaaac gccggcagca ccggcgacag atggcgcagc ccgaacgaca tcggcgcatt cacgcgcacg aggccgtgcg gctcgagcgc ctgcgcggac gcttcgttct cgatcagctc cgcatcggcg agcaggcgga tcgcgcggtc gcgcagcagt tcgccggtcg gcgtgagcga cagcttgcgc gacgtgcggt acagcagcat cgcgccgagg cgtttttcga ggcgcgcgat cgccttcgac acggtgggtt gcgatacgcc gagcaggtcg gcggctttcg cgaacgagcc ggtctccgcg acgcgcgcaa agatggccca ggcttcgaga tcaggaaggt gttgcatcga accaggaccc tgggtctgag cttccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQHLPDLEA WAIFARVAET GSFAKAADLL GVSQPTVSKA IARLEKRLGA MLLYRTSRKL SLTPTGELLR DRAIRLLADA ELIENEASAQ ALEPHGLVRV NAPMSFGLRH LSPVLPAFLE RYPRVDIDLV LTDHIVDIVS GGFDVAIRIA ELNDSTLRSR RLCAVRRPLV ASPAYLDRRG RPTHPRDIEQ HVCLTYTNLP TPEHWRFRHP ASGDEVGVSV HGRIRTNNAD VILPALVGGH GLALQPEFLV WDAMQRGELE EVMSDWKIAD INVNLVTPPG MLRAARVTVL LDFLAERFAH APWALPAA
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCAACACCTT CCTGATCTCG AAGCCTGGGC CATCTTTGCG CGCGTCGCGG AGACCGGCTC GTTCGCGAAA GCCGCCGACC TGCTCGGCGT ATCGCAACCC ACCGTGTCGA AGGCGATCGC GCGCCTCGAA AAACGCCTCG GCGCGATGCT GCTGTACCGC ACGTCGCGCA AGCTGTCGCT CACGCCGACC GGCGAACTGC TGCGCGACCG CGCGATCCGC CTGCTCGCCG ATGCGGAGCT GATCGAGAAC GAAGCGTCCG CGCAGGCGCT CGAGCCGCAC GGCCTCGTGC GCGTGAATGC GCCGATGTCG TTCGGGCTGC GCCATCTGTC GCCGGTGCTG CCGGCGTTTC TCGAACGCTA TCCGCGCGTC GACATCGATC TCGTGCTCAC CGATCACATC GTCGACATCG TGTCGGGCGG CTTCGACGTC GCGATCCGCA TCGCCGAGCT GAACGATTCG ACGCTGCGTT CGCGCCGCCT CTGCGCGGTG CGCCGGCCGC TCGTCGCATC GCCCGCGTAT CTCGACCGGC GCGGCCGCCC CACGCATCCG CGCGACATCG AACAGCACGT GTGCCTCACG TACACCAACT TGCCGACCCC CGAGCACTGG CGCTTCCGCC ATCCGGCGTC GGGCGACGAA GTCGGCGTGT CCGTCCACGG GCGCATCCGC ACCAACAACG CGGACGTGAT CCTCCCCGCG CTCGTCGGCG GTCACGGCCT GGCGCTGCAG CCCGAGTTCC TCGTGTGGGA CGCGATGCAG CGCGGCGAGC TCGAGGAAGT GATGAGCGAC TGGAAGATCG CGGACATCAA CGTGAACCTC GTCACGCCGC CCGGCATGCT GCGCGCCGCG CGCGTGACGG TGCTGCTCGA CTTCCTCGCG GAACGCTTCG CGCACGCGCC GTGGGCGCTG CCCGCCGCCT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA