SSGCID: Target Page

BuceA.12800.a

LysR family regulatory protein

CENTER ID:	BuceA.12800.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.12800.a.A1.GE31856	Full length( BuceA.12800.a )		1	307

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.1389
RefSeq:	YP_002230473.1
UniProt:	B4E664

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDKWTQIEFF VQVAELGSLS KAAERLGMSS AAASRCLNAL EERLGARLVE RTTRRLWLTD AGHEYHRRCV AILNELSEAD AAVNEASVIP AGTLRVTASV SFASIYIAPA LPEFHRRYPN LNVQIVAANR YPDFIEAGID VAIRTREHEA DSGITVRKLA ETRRVLAASP GYLAKHGAPA TPDALGDHRL LVYNLANDPY VLHFRHGAKK QSVTIKSVLD ANEGQVIRAA ALAGLGILIQ PLYIIHDDIV AGKLVPVLTD WQLPALTVNI AYQSRRHQPA KIRVFTEFLI ERFERLGLAR KWKEVGR

NT Sequence

ATGGACAAGT GGACGCAGAT CGAGTTTTTC GTACAGGTGG CGGAGCTCGG CAGCCTGTCG AAGGCTGCCG AGCGGCTCGG CATGTCGAGC GCGGCGGCGA GCCGCTGCCT GAACGCGCTG GAAGAGCGGC TGGGCGCGCG GCTCGTCGAG CGGACCACGC GCCGGCTGTG GCTGACCGAC GCCGGGCACG AGTACCACCG GCGTTGTGTC GCGATCCTCA ACGAGCTGTC GGAAGCGGAC GCGGCCGTCA ACGAAGCGAG CGTCATTCCG GCCGGTACGC TGCGCGTGAC CGCGTCGGTG TCGTTCGCGT CGATCTACAT CGCGCCCGCG CTGCCCGAAT TTCACCGGCG CTACCCGAAC CTGAACGTGC AGATCGTCGC GGCGAACCGT TACCCGGATT TCATCGAGGC CGGCATCGAC GTGGCGATCC GCACGCGCGA ACACGAAGCG GATTCGGGCA TCACCGTGCG CAAGCTCGCC GAGACACGGC GCGTGCTGGC CGCGTCGCCC GGGTATCTGG CGAAGCACGG CGCGCCGGCG ACGCCCGATG CGCTGGGCGA TCACCGGCTG CTCGTCTACA ACCTCGCGAA CGATCCATAC GTGCTGCATT TCCGCCATGG TGCGAAGAAG CAGTCGGTCA CGATCAAGAG CGTGCTCGAT GCGAACGAAG GGCAGGTGAT CCGCGCGGCG GCGCTCGCCG GGCTCGGCAT CCTGATCCAG CCGCTTTACA TCATTCACGA CGATATCGTC GCCGGGAAGC TGGTGCCGGT GCTGACCGAC TGGCAACTGC CCGCGCTCAC CGTCAACATC GCGTACCAGA GCCGCCGACA CCAGCCGGCG AAGATCCGCG TCTTCACCGA GTTCCTGATC GAGCGTTTCG AACGACTCGG ACTCGCGCGC AAATGGAAGG AAGTCGGCCG C

Details for BuceA.12800.a.A1.GE31856

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	36kDa
OBSERVED MW:	36kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	93

Validated AA Sequence

Validated NT Sequence

ggccgacttc cttccatttg cgcgcgagtc cgagtcgttc gaaacgctcg atcaggaact cggtgaagac gcggatcttc gccggctggt gtcggcggct ctggtacgcg atgttgacgg tgagcgcggg cagttgccag tcggtcagca ccggcaccag cttcccggcg acgatatcgt cgtgaatgat gtaaagcggc tggatcagga tgccgagccc ggcgagcgcc gccgcgcgga tcacctgccc ttcgttcgca tcgagcacgc tcttgatcgt gaccgactgc ttcttcgcac catggcggaa atgcagcacg tatggatcgt tcgcgaggtt gtagacgagc agccggtgat cgcccagcgc atcgggcgtc gccggcgcgc cgtgcttcgc cagatacccg ggcgacgcgg ccagcacgcg ccgtgtctcg gcgagcttgc gcacggtgat gcccgaatcc gcttcgtgtt cgcgcgtgcg gatcgccacg tcgatgccgg cctcgatgaa atccgggtaa cggttcgccg cgacgatctg cacgttcagg ttcgggtagc gccggtgaaa ttcgggcagc gcgggcgcga tgtagatcga cgcgaacgac accgacgcgg tcacgcgcag cgtaccggcc ggaatgacgc tcgcttcgtt gacggccgcg tccgcttccg acagctcgtt gaggatcgcg acacaacgcc ggtggtactc gtgcccggcg tcggtcagcc acagccggcg cgtggtccgc tcgacgagcc gcgcgcccag ccgctcttcc agcgcgttca ggcagcggct cgccgccgcg ctcgacatgc cgagccgctc ggcagccttc gacaggctgc cgagctccgc cacctgtacg aaaaactcga tctgcgtcca cttgtccatc gaaccaggac cctgggtctg agcttccagg gtaccca

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDKWTQIEF FVQVAELGSL SKAAERLGMS SAAASRCLNA LEERLGARLV ERTTRRLWLT DAGHEYHRRC VAILNELSEA DAAVNEASVI PAGTLRVTAS VSFASIYIAP ALPEFHRRYP NLNVQIVAAN RYPDFIEAGI DVAIRTREHE ADSGITVRKL AETRRVLAAS PGYLAKHGAP ATPDALGDHR LLVYNLANDP YVLHFRHGAK KQSVTIKSVL DANEGQVIRA AALAGLGILI QPLYIIHDDI VAGKLVPVLT DWQLPALTVN IAYQSRRHQP AKIRVFTEFL IERFERLGLA RKWKEVGR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaagtgg acgcagatcg agtttttcgt acaggtggcg gagctcggca gcctgtcgaa ggctgccgag cggctcggca tgtcgagcgc ggcggcgagc cgctgcctga acgcgctgga agagcggctg ggcgcgcggc tcgtcgagcg gaccacgcgc cggctgtggc tgaccgacgc cgggcacgag taccaccggc gttgtgtcgc gatcctcaac gagctgtcgg aagcggacgc ggccgtcaac gaagcgagcg tcattccggc cggtacgctg cgcgtgaccg cgtcggtgtc gttcgcgtcg atctacatcg cgcccgcgct gcccgaattt caccggcgct acccgaacct gaacgtgcag atcgtcgcgg cgaaccgtta cccggatttc atcgaggccg gcatcgacgt ggcgatccgc acgcgcgaac acgaagcgga ttcgggcatc accgtgcgca agctcgccga gacacggcgc gtgctggccg cgtcgcccgg gtatctggcg aagcacggcg cgccggcgac gcccgatgcg ctgggcgatc accggctgct cgtctacaac ctcgcgaacg atccatacgt gctgcatttc cgccatggtg cgaagaagca gtcggtcacg atcaagagcg tgctcgatgc gaacgaaggg caggtgatcc gcgcggcggc gctcgccggg ctcggcatcc tgatccagcc gctttacatc attcacgacg atatcgtcgc cgggaagctg gtgccggtgc tgaccgactg gcaactgccc gcgctcaccg tcaacatcgc gtaccagagc cgccgacacc agccggcgaa gatccgcgtc ttcaccgagt tcctgatcga gcgtttcgaa cgactcggac tcgcgcgcaa atggaaggaa gtcggccgct aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa