BuceA.12746.a
Metallo-beta-lactamase superfamily protein
| CENTER ID: | BuceA.12746.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.12746.a.B1.GE37736 | Full length( BuceA.12746.a ) | 1 | 319 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.12746.a.B1.PS02032 | Full length( BuceA.12746.a ) | 1 | 319 |
Structures
8D2Z
DEPOSITED: 5/31/2022
DETERMINATION: XRay
CLONE: BuceA.12746.a.B1.GE37736
PROTEIN: BuceA.12746.a.B1.PS02032
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_140167 |
| PATRIC ID: | fig|216591.5.peg.5176 |
| RefSeq: | YP_002234045.1 |
| UniProt: | B4EIY5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAKAFASQAD LEEKKVTWTK LSENAYAYTA EGDPNSGVII GDDSVLIVDT TATPAMAQDL IAKIRSVTDK PIKHVVLSHY HAVRVLGASA YFDEGAQHVI ASRGTYEMIV ERGEADMKSE IERFPRLFAG VETVPGLTWP TLVFEREITL FLGKLEVKIM HVGSGHTKGD TIVWLPSQKV LFSGDLVEYD AACYCGDAQL EQWPATLEAL RALGAEKLVP GRGPALLNPA EVNKGLDYTK DFVTTLLAQG RKAVERNLDL KAAMALTREA MDPKFGHVFI YEHCLPFDVS RAFDEASGIA HPRIWTAQRD KDMWAALQD
NT Sequence
ATGGCCAAAG CATTCGCCTC CCAAGCCGAT CTGGAAGAGA AGAAAGTCAC CTGGACGAAG CTGTCCGAGA ACGCCTACGC GTACACAGCC GAAGGCGACC CGAACTCGGG CGTGATCATC GGCGACGACA GTGTGCTGAT CGTCGACACG ACCGCGACGC CCGCGATGGC GCAGGACCTG ATCGCGAAGA TCCGCAGCGT CACCGACAAG CCGATCAAGC ACGTGGTGCT GTCGCACTAC CACGCGGTGC GCGTGCTCGG CGCGTCCGCG TATTTCGACG AAGGCGCGCA GCACGTGATC GCGAGCCGCG GCACGTACGA GATGATCGTC GAGCGCGGCG AGGCCGACAT GAAGTCGGAG ATCGAGCGCT TCCCGCGCCT GTTCGCCGGC GTCGAGACGG TGCCGGGCCT GACCTGGCCG ACGCTCGTGT TCGAGCGCGA GATCACGCTG TTCCTCGGCA AGCTCGAAGT GAAGATCATG CACGTCGGCT CGGGCCACAC GAAGGGCGAC ACGATCGTCT GGCTGCCGTC GCAGAAGGTG CTGTTCTCGG GCGACCTTGT CGAATACGAC GCCGCGTGCT ATTGCGGCGA CGCGCAGCTC GAACAGTGGC CGGCCACGCT CGAGGCGCTG CGCGCGCTCG GCGCCGAGAA GCTCGTGCCG GGCCGCGGCC CCGCGCTGCT GAATCCGGCG GAAGTGAACA AGGGCCTCGA CTACACGAAG GATTTCGTCA CGACGCTGCT CGCGCAGGGC CGCAAGGCCG TCGAGCGCAA TCTCGACCTG AAGGCCGCGA TGGCGCTCAC GCGCGAAGCG ATGGACCCGA AGTTCGGCCA CGTGTTCATC TACGAGCACT GTCTGCCGTT CGACGTGTCG CGTGCGTTCG ACGAGGCGAG CGGCATCGCG CATCCGCGCA TCTGGACCGC GCAGCGCGAC AAGGACATGT GGGCCGCGCT GCAGGAC
Details for BuceA.12746.a.B1.GE37736
| HARVESTED ON: | 2/5/2014 |
| SEQUENCED ON: | 2/6/2014 |
| EXPECTED MW: | 36kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA KAFASQADLE EKKVTWTKLS ENAYAYTAEG DPNSGVIIGD DSVLIVDTTA TPAMAQDLIA KIRSVTDKPI KHVVLSHYHA VRVLGASAYF DEGAQHVIAS RGTYEMIVER GEADMKSEIE RFPRLFAGVE TVPGLTWPTL VFEREITLFL GKLEVKIMHV GSGHTKGDTI VWLPSQKVLF SGDLVEYDAA CYCGDAQLEQ WPATLEALRA LGAEKLVPGR GPALLNPAEV NKGLDYTKDF VTTLLAQGRK AVERNLDLKA AMALTREAMD PKFGHVFIYE HCLPFDVSRA FDEASGIAHP RIWTAQRDKD MWAALQD
Validated NT Sequence
cttagtcctg cagcgcggcc cacatgtcct tgtcgcgctg cgcggtccag atgcgcggat gcgcgatgcc gctcgcctcg tcgaacgcac gcgacacgtc gaacggcaga cagtgctcgt agatgaacac gtggccgaac ttcgggtcca tcgcttcgcg cgtgagcgcc atcgcggcct tcaggtcgag attgcgctcg acggccttgc ggccctgcgc gagcagcgtc gtgacgaaat ccttcgtgta gtcgaggccc ttgttcactt ccgccggatt cagcagcgcg gggccgcggc ccggcacgag cttctcggcg ccgagcgcgc gcagcgcctc gagcgtggcc ggccactgtt cgagctgcgc gtcgccgcaa tagcacgcgg cgtcgtattc gacaaggtcg cccgagaaca gcaccttctg cgacggcagc cagacgatcg tgtcgccctt cgtgtggccc gagccgacgt gcatgatctt cacttcgagc ttgccgagga acagcgtgat ctcgcgctcg aacacgagcg tcggccaggt caggcccggc accgtctcga cgccggcgaa caggcgcggg aagcgctcga tctccgactt catgtcggcc tcgccgcgct cgacgatcat ctcgtacgtg ccgcggctcg cgatcacgtg ctgcgcgcct tcgtcgaaat acgcggacgc gccgagcacg cgcaccgcgt ggtagtgcga cagcaccacg tgcttgatcg gcttgtcggt gacgctgcgg atcttcgcga tcaggtcctg cgccatcgcg ggcgtcgcgg tcgtgtcgac gatcagcaca ctgtcgtcgc cgatgatcac gcccgagttc gggtcgcctt cggctgtgta cgcgtaggcg ttctcggaca gcttcgtcca ggtgactttc ttctcttcca gatcggcttg ggaggcgaat gctttggcca tatggtggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMA KAFASQADLE EKKVTWTKLS ENAYAYTAEG DPNSGVIIGD DSVLIVDTTA TPAMAQDLIA KIRSVTDKPI KHVVLSHYHA VRVLGASAYF DEGAQHVIAS RGTYEMIVER GEADMKSEIE RFPRLFAGVE TVPGLTWPTL VFEREITLFL GKLEVKIMHV GSGHTKGDTI VWLPSQKVLF SGDLVEYDAA CYCGDAQLEQ WPATLEALRA LGAEKLVPGR GPALLNPAEV NKGLDYTKDF VTTLLAQGRK AVERNLDLKA AMALTREAMD PKFGHVFIYE HCLPFDVSRA FDEASGIAHP RIWTAQRDKD MWAALQD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcca aagcattcgc ctcccaagcc gatctggaag agaagaaagt cacctggacg aagctgtccg agaacgccta cgcgtacaca gccgaaggcg acccgaactc gggcgtgatc atcggcgacg acagtgtgct gatcgtcgac acgaccgcga cgcccgcgat ggcgcaggac ctgatcgcga agatccgcag cgtcaccgac aagccgatca agcacgtggt gctgtcgcac taccacgcgg tgcgcgtgct cggcgcgtcc gcgtatttcg acgaaggcgc gcagcacgtg atcgcgagcc gcggcacgta cgagatgatc gtcgagcgcg gcgaggccga catgaagtcg gagatcgagc gcttcccgcg cctgttcgcc ggcgtcgaga cggtgccggg cctgacctgg ccgacgctcg tgttcgagcg cgagatcacg ctgttcctcg gcaagctcga agtgaagatc atgcacgtcg gctcgggcca cacgaagggc gacacgatcg tctggctgcc gtcgcagaag gtgctgttct cgggcgacct tgtcgaatac gacgccgcgt gctattgcgg cgacgcgcag ctcgaacagt ggccggccac gctcgaggcg ctgcgcgcgc tcggcgccga gaagctcgtg ccgggccgcg gccccgcgct gctgaatccg gcggaagtga acaagggcct cgactacacg aaggatttcg tcacgacgct gctcgcgcag ggccgcaagg ccgtcgagcg caatctcgac ctgaaggccg cgatggcgct cacgcgcgaa gcgatggacc cgaagttcgg ccacgtgttc atctacgagc actgtctgcc gttcgacgtg tcgcgtgcgt tcgacgaggc gagcggcatc gcgcatccgc gcatctggac cgcgcagcgc gacaaggaca tgtgggccgc gctgcaggac tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuceA.12746.a.B1.PS02032
| PURIFICATION DATe: | 3/27/2014 |
| CONCENTRATION: | 72.9mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA KAFASQADLE EKKVTWTKLS ENAYAYTAEG DPNSGVIIGD DSVLIVDTTA TPAMAQDLIA KIRSVTDKPI KHVVLSHYHA VRVLGASAYF DEGAQHVIAS RGTYEMIVER GEADMKSEIE RFPRLFAGVE TVPGLTWPTL VFEREITLFL GKLEVKIMHV GSGHTKGDTI VWLPSQKVLF SGDLVEYDAA CYCGDAQLEQ WPATLEALRA LGAEKLVPGR GPALLNPAEV NKGLDYTKDF VTTLLAQGRK AVERNLDLKA AMALTREAMD PKFGHVFIYE HCLPFDVSRA FDEASGIAHP RIWTAQRDKD MWAALQD
Validated NT Sequence
cttagtcctg cagcgcggcc cacatgtcct tgtcgcgctg cgcggtccag atgcgcggat gcgcgatgcc gctcgcctcg tcgaacgcac gcgacacgtc gaacggcaga cagtgctcgt agatgaacac gtggccgaac ttcgggtcca tcgcttcgcg cgtgagcgcc atcgcggcct tcaggtcgag attgcgctcg acggccttgc ggccctgcgc gagcagcgtc gtgacgaaat ccttcgtgta gtcgaggccc ttgttcactt ccgccggatt cagcagcgcg gggccgcggc ccggcacgag cttctcggcg ccgagcgcgc gcagcgcctc gagcgtggcc ggccactgtt cgagctgcgc gtcgccgcaa tagcacgcgg cgtcgtattc gacaaggtcg cccgagaaca gcaccttctg cgacggcagc cagacgatcg tgtcgccctt cgtgtggccc gagccgacgt gcatgatctt cacttcgagc ttgccgagga acagcgtgat ctcgcgctcg aacacgagcg tcggccaggt caggcccggc accgtctcga cgccggcgaa caggcgcggg aagcgctcga tctccgactt catgtcggcc tcgccgcgct cgacgatcat ctcgtacgtg ccgcggctcg cgatcacgtg ctgcgcgcct tcgtcgaaat acgcggacgc gccgagcacg cgcaccgcgt ggtagtgcga cagcaccacg tgcttgatcg gcttgtcggt gacgctgcgg atcttcgcga tcaggtcctg cgccatcgcg ggcgtcgcgg tcgtgtcgac gatcagcaca ctgtcgtcgc cgatgatcac gcccgagttc gggtcgcctt cggctgtgta cgcgtaggcg ttctcggaca gcttcgtcca ggtgactttc ttctcttcca gatcggcttg ggaggcgaat gctttggcca tatggtggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMA KAFASQADLE EKKVTWTKLS ENAYAYTAEG DPNSGVIIGD DSVLIVDTTA TPAMAQDLIA KIRSVTDKPI KHVVLSHYHA VRVLGASAYF DEGAQHVIAS RGTYEMIVER GEADMKSEIE RFPRLFAGVE TVPGLTWPTL VFEREITLFL GKLEVKIMHV GSGHTKGDTI VWLPSQKVLF SGDLVEYDAA CYCGDAQLEQ WPATLEALRA LGAEKLVPGR GPALLNPAEV NKGLDYTKDF VTTLLAQGRK AVERNLDLKA AMALTREAMD PKFGHVFIYE HCLPFDVSRA FDEASGIAHP RIWTAQRDKD MWAALQD
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCCA AAGCATTCGC CTCCCAAGCC GATCTGGAAG AGAAGAAAGT CACCTGGACG AAGCTGTCCG AGAACGCCTA CGCGTACACA GCCGAAGGCG ACCCGAACTC GGGCGTGATC ATCGGCGACG ACAGTGTGCT GATCGTCGAC ACGACCGCGA CGCCCGCGAT GGCGCAGGAC CTGATCGCGA AGATCCGCAG CGTCACCGAC AAGCCGATCA AGCACGTGGT GCTGTCGCAC TACCACGCGG TGCGCGTGCT CGGCGCGTCC GCGTATTTCG ACGAAGGCGC GCAGCACGTG ATCGCGAGCC GCGGCACGTA CGAGATGATC GTCGAGCGCG GCGAGGCCGA CATGAAGTCG GAGATCGAGC GCTTCCCGCG CCTGTTCGCC GGCGTCGAGA CGGTGCCGGG CCTGACCTGG CCGACGCTCG TGTTCGAGCG CGAGATCACG CTGTTCCTCG GCAAGCTCGA AGTGAAGATC ATGCACGTCG GCTCGGGCCA CACGAAGGGC GACACGATCG TCTGGCTGCC GTCGCAGAAG GTGCTGTTCT CGGGCGACCT TGTCGAATAC GACGCCGCGT GCTATTGCGG CGACGCGCAG CTCGAACAGT GGCCGGCCAC GCTCGAGGCG CTGCGCGCGC TCGGCGCCGA GAAGCTCGTG CCGGGCCGCG GCCCCGCGCT GCTGAATCCG GCGGAAGTGA ACAAGGGCCT CGACTACACG AAGGATTTCG TCACGACGCT GCTCGCGCAG GGCCGCAAGG CCGTCGAGCG CAATCTCGAC CTGAAGGCCG CGATGGCGCT CACGCGCGAA GCGATGGACC CGAAGTTCGG CCACGTGTTC ATCTACGAGC ACTGTCTGCC GTTCGACGTG TCGCGTGCGT TCGACGAGGC GAGCGGCATC GCGCATCCGC GCATCTGGAC CGCGCAGCGC GACAAGGACA TGTGGGCCGC GCTGCAGGAC TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT