BuceA.12245.d

Metallo peptidase, family M20 unassigned

CENTER ID: BuceA.12245.d
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12245.d.B1.GE37734 Full length( BuceA.12245.d ) 1 429
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12245.d.B1.PS02048 Full length( BuceA.12245.d ) 1 429

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.6833
RefSeq: YP_002153538.1
UniProt: B4EPR2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQVQDVTPGV AASGLRVDGA RLWDSLMRLA RIGATDKGGV CRLALTDLDR QARDLFVAWA KDIGCTVRVD AIGNIFARRA GERDDLPPVA TGSHIDTQPT GGKFDGNYGV LAGLEVLRTL DAAGVRTRAP LEVAVWTNEE GSRFVPVMMG SGVFAGAFTL DHALAQCDRD GVSVRDALAA IGYAGSADDG RGAHPVGAYF EAHIEQGPVL EAHDTTIGVV EGALGQRWYD VTVHGMEAHA GPTPMELRRD ALLVAADLVR AVNGIARAHP PHGRGTVGWI DVHPNSRNVI PGRVTLTVDL RAADDATLTA MDAQLRAACA DLGAAAGMRI DIEPVVYFPP QPFDPALVEQ VRAGARALGL SSMNVISGAG HDAVYLARVA PTAMIFVPCK DGISHNEIED ADPAHLEAGC NVLLHAMLGA AGIAGGQAR
NT Sequence
ATGCAAGTAC AGGATGTGAC TCCGGGCGTC GCGGCGTCCG GCTTGCGCGT CGATGGCGCG CGGCTGTGGG ACAGCCTGAT GCGGCTCGCG CGGATCGGCG CGACCGACAA GGGCGGCGTG TGCCGCCTCG CGCTCACGGA CCTCGATCGC CAGGCGCGCG ACCTGTTCGT CGCGTGGGCG AAGGACATCG GTTGCACGGT GCGCGTCGAT GCGATCGGCA ATATCTTCGC GCGGCGCGCG GGCGAGCGGG ACGACCTGCC GCCCGTGGCG ACGGGCAGCC ATATCGACAC GCAGCCGACC GGCGGCAAGT TCGACGGCAA CTACGGCGTG CTGGCCGGCC TCGAGGTGCT GCGTACGCTC GACGCCGCCG GGGTGCGCAC GCGCGCGCCG CTCGAGGTGG CCGTCTGGAC CAACGAAGAA GGGTCGCGCT TCGTCCCGGT GATGATGGGC TCCGGCGTAT TCGCGGGCGC GTTCACGCTC GACCATGCGC TCGCGCAATG CGATCGCGAT GGCGTGTCGG TGCGCGATGC GCTGGCCGCG ATCGGGTACG CGGGCAGTGC GGACGACGGG CGCGGCGCGC ATCCCGTCGG CGCGTATTTC GAAGCGCATA TCGAGCAGGG GCCGGTGCTG GAGGCGCACG ACACGACGAT CGGCGTGGTC GAGGGCGCGC TCGGACAACG CTGGTACGAC GTGACCGTGC ACGGGATGGA GGCGCATGCC GGCCCCACGC CGATGGAACT GCGCCGCGAT GCGCTGCTGG TCGCGGCCGA TCTCGTGCGC GCCGTGAACG GCATCGCGCG CGCGCATCCG CCGCACGGAC GCGGCACGGT CGGCTGGATC GACGTGCACC CGAATTCGCG CAACGTGATT CCCGGCCGCG TGACGCTGAC CGTCGACCTG CGCGCGGCCG ACGACGCGAC GCTGACGGCG ATGGACGCGC AACTGCGCGC GGCATGCGCC GACCTGGGCG CGGCGGCCGG GATGCGGATC GACATCGAGC CGGTCGTGTA TTTTCCGCCG CAGCCGTTCG ATCCGGCGCT GGTCGAGCAG GTCCGCGCCG GCGCGCGCGC GCTCGGGCTG TCGTCGATGA ACGTGATCAG CGGCGCGGGC CACGATGCGG TGTATCTCGC GCGCGTGGCG CCGACCGCGA TGATCTTCGT GCCGTGCAAG GACGGCATCA GCCACAACGA GATCGAAGAT GCCGATCCGG CGCATCTGGA AGCCGGCTGC AACGTGCTGC TGCACGCGAT GCTGGGCGCC GCCGGGATCG CGGGGGGCCA AGCGCGA
Details for BuceA.12245.d.B1.GE37734
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 24
PERCENT COVERAGE: 91
Validated AA Sequence
MRRIGIFDLV VADAVLARHE DHRGRRHARE IHRIVARAAD HVHRRQPERA RAGADLLDQR RIERLRRKIH DRLDVDPHPG RRAQVGACRA QLRVHRRQRR VVGRAQVDGQ RHAAGNHVAR IRVHVDPADR AASVRRMRAR DAVHGAHEIG RDQQRIAAQF HRRGAGMRLH PVHGHVVPAL SERALDHADR RVVRLQHRPL LDMRFEIRAD GMRAAPVVRT ARVPDRGQRI AHRHAIAIAL RERMVERERA REYAGAHHHR DEARPFFVGP DGHLERRARA HPGGVERTQH LEAGQHAVVA VELAAGRLRV DMAARRHGRQ VVPLARAPRE DIADRIDAHR ATDVLRPRDE QVARLAIEVR EREAAHAALV GRADPREPHQ AVPQPRAIDA QAGRRDARSH I
Validated NT Sequence
cggcggcgcc cagcatcgcg tgcagcagca cgttgcagcc ggcttccaga tgcgccggat cggcatcttc gatctcgttg tggctgatgc cgtccttgca cggcacgaag atcatcgcgg tcggcgccac gcgcgcgaga tacaccgcat cgtggcccgc gccgctgatc acgttcatcg acgacagccc gagcgcgcgc gcgccggcgc ggacctgctc gaccagcgcc ggatcgaacg gctgcggcgg aaaatacacg accggctcga tgtcgatccg catcccggcc gccgcgccca ggtcggcgca tgccgcgcgc agttgcgcgt ccatcgccgt cagcgtcgcg tcgtcggccg cgcgcaggtc gacggtcagc gtcacgcggc cgggaatcac gttgcgcgaa ttcgggtgca cgtcgatcca gccgaccgtg ccgcgtccgt gcggcggatg cgcgcgcgcg atgccgttca cggcgcgcac gagatcggcc gcgaccagca gcgcatcgcg gcgcagttcc atcggcgtgg ggccggcatg cgcctccatc ccgtgcacgg tcacgtcgta ccagcgttgt ccgagcgcgc cctcgaccac gccgatcgtc gtgtcgtgcg cctccagcac cggcccctgc tcgatatgcg cttcgaaata cgcgccgacg ggatgcgcgc cgcgcccgtc gtccgcactg cccgcgtacc cgatcgcggc cagcgcatcg cgcaccgaca cgccatcgcg atcgcattgc gcgagcgcat ggtcgagcgt gaacgcgccc gcgaatacgc cggagcccat catcaccggg acgaagcgcg acccttcttc gttggtccag acggccacct cgagcggcgc gcgcgtgcgc accccggcgg cgtcgagcgt acgcagcacc tcgaggccgg ccagcacgcc gtagttgccg tcgaacttgc cgccggtcgg ctgcgtgtcg atatggctgc ccgtcgccac gggcggcagg tcgtcccgct cgcccgcgcg ccgcgcgaag atattgccga tcgcatcgac gcgcaccgtg caaccgatgt ccttcgccca cgcgacgaac aggtcgcgcg cctggcgatc gaggtccgtg agcgcgaggc ggcacacgcc gcccttgtcg gtcgcgccga tccgcgcgag ccgcatcagg ctgtcccaca gccgcgcgcc atcgacgcgc aagccggacg ccgcgacgcc cggagtcaca tcct
Expected Protein Sequence
MAHHHHHHMQ VQDVTPGVAA SGLRVDGARL WDSLMRLARI GATDKGGVCR LALTDLDRQA RDLFVAWAKD IGCTVRVDAI GNIFARRAGE RDDLPPVATG SHIDTQPTGG KFDGNYGVLA GLEVLRTLDA AGVRTRAPLE VAVWTNEEGS RFVPVMMGSG VFAGAFTLDH ALAQCDRDGV SVRDALAAIG YAGSADDGRG AHPVGAYFEA HIEQGPVLEA HDTTIGVVEG ALGQRWYDVT VHGMEAHAGP TPMELRRDAL LVAADLVRAV NGIARAHPPH GRGTVGWIDV HPNSRNVIPG RVTLTVDLRA ADDATLTAMD AQLRAACADL GAAAGMRIDI EPVVYFPPQP FDPALVEQVR AGARALGLSS MNVISGAGHD AVYLARVAPT AMIFVPCKDG ISHNEIEDAD PAHLEAGCNV LLHAMLGAAG IAGGQAR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcaag tacaggatgt gactccgggc gtcgcggcgt ccggcttgcg cgtcgatggc gcgcggctgt gggacagcct gatgcggctc gcgcggatcg gcgcgaccga caagggcggc gtgtgccgcc tcgcgctcac ggacctcgat cgccaggcgc gcgacctgtt cgtcgcgtgg gcgaaggaca tcggttgcac ggtgcgcgtc gatgcgatcg gcaatatctt cgcgcggcgc gcgggcgagc gggacgacct gccgcccgtg gcgacgggca gccatatcga cacgcagccg accggcggca agttcgacgg caactacggc gtgctggccg gcctcgaggt gctgcgtacg ctcgacgccg ccggggtgcg cacgcgcgcg ccgctcgagg tggccgtctg gaccaacgaa gaagggtcgc gcttcgtccc ggtgatgatg ggctccggcg tattcgcggg cgcgttcacg ctcgaccatg cgctcgcgca atgcgatcgc gatggcgtgt cggtgcgcga tgcgctggcc gcgatcgggt acgcgggcag tgcggacgac gggcgcggcg cgcatcccgt cggcgcgtat ttcgaagcgc atatcgagca ggggccggtg ctggaggcgc acgacacgac gatcggcgtg gtcgagggcg cgctcggaca acgctggtac gacgtgaccg tgcacgggat ggaggcgcat gccggcccca cgccgatgga actgcgccgc gatgcgctgc tggtcgcggc cgatctcgtg cgcgccgtga acggcatcgc gcgcgcgcat ccgccgcacg gacgcggcac ggtcggctgg atcgacgtgc acccgaattc gcgcaacgtg attcccggcc gcgtgacgct gaccgtcgac ctgcgcgcgg ccgacgacgc gacgctgacg gcgatggacg cgcaactgcg cgcggcatgc gccgacctgg gcgcggcggc cgggatgcgg atcgacatcg agccggtcgt gtattttccg ccgcagccgt tcgatccggc gctggtcgag caggtccgcg ccggcgcgcg cgcgctcggg ctgtcgtcga tgaacgtgat cagcggcgcg ggccacgatg cggtgtatct cgcgcgcgtg gcgccgaccg cgatgatctt cgtgccgtgc aaggacggca tcagccacaa cgagatcgaa gatgccgatc cggcgcatct ggaagccggc tgcaacgtgc tgctgcacgc gatgctgggc gccgccggga tcgcgggggg ccaagcgcga tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for BuceA.12245.d.B1.PS02048
PURIFICATION DATe: 4/29/2014
CONCENTRATION: 51.29mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 24
PERCENT COVERAGE: 91
Protocol Notes
notes unavailable
Validated AA Sequence
MRRIGIFDLV VADAVLARHE DHRGRRHARE IHRIVARAAD HVHRRQPERA RAGADLLDQR RIERLRRKIH DRLDVDPHPG RRAQVGACRA QLRVHRRQRR VVGRAQVDGQ RHAAGNHVAR IRVHVDPADR AASVRRMRAR DAVHGAHEIG RDQQRIAAQF HRRGAGMRLH PVHGHVVPAL SERALDHADR RVVRLQHRPL LDMRFEIRAD GMRAAPVVRT ARVPDRGQRI AHRHAIAIAL RERMVERERA REYAGAHHHR DEARPFFVGP DGHLERRARA HPGGVERTQH LEAGQHAVVA VELAAGRLRV DMAARRHGRQ VVPLARAPRE DIADRIDAHR ATDVLRPRDE QVARLAIEVR EREAAHAALV GRADPREPHQ AVPQPRAIDA QAGRRDARSH I
Validated NT Sequence
cggcggcgcc cagcatcgcg tgcagcagca cgttgcagcc ggcttccaga tgcgccggat cggcatcttc gatctcgttg tggctgatgc cgtccttgca cggcacgaag atcatcgcgg tcggcgccac gcgcgcgaga tacaccgcat cgtggcccgc gccgctgatc acgttcatcg acgacagccc gagcgcgcgc gcgccggcgc ggacctgctc gaccagcgcc ggatcgaacg gctgcggcgg aaaatacacg accggctcga tgtcgatccg catcccggcc gccgcgccca ggtcggcgca tgccgcgcgc agttgcgcgt ccatcgccgt cagcgtcgcg tcgtcggccg cgcgcaggtc gacggtcagc gtcacgcggc cgggaatcac gttgcgcgaa ttcgggtgca cgtcgatcca gccgaccgtg ccgcgtccgt gcggcggatg cgcgcgcgcg atgccgttca cggcgcgcac gagatcggcc gcgaccagca gcgcatcgcg gcgcagttcc atcggcgtgg ggccggcatg cgcctccatc ccgtgcacgg tcacgtcgta ccagcgttgt ccgagcgcgc cctcgaccac gccgatcgtc gtgtcgtgcg cctccagcac cggcccctgc tcgatatgcg cttcgaaata cgcgccgacg ggatgcgcgc cgcgcccgtc gtccgcactg cccgcgtacc cgatcgcggc cagcgcatcg cgcaccgaca cgccatcgcg atcgcattgc gcgagcgcat ggtcgagcgt gaacgcgccc gcgaatacgc cggagcccat catcaccggg acgaagcgcg acccttcttc gttggtccag acggccacct cgagcggcgc gcgcgtgcgc accccggcgg cgtcgagcgt acgcagcacc tcgaggccgg ccagcacgcc gtagttgccg tcgaacttgc cgccggtcgg ctgcgtgtcg atatggctgc ccgtcgccac gggcggcagg tcgtcccgct cgcccgcgcg ccgcgcgaag atattgccga tcgcatcgac gcgcaccgtg caaccgatgt ccttcgccca cgcgacgaac aggtcgcgcg cctggcgatc gaggtccgtg agcgcgaggc ggcacacgcc gcccttgtcg gtcgcgccga tccgcgcgag ccgcatcagg ctgtcccaca gccgcgcgcc atcgacgcgc aagccggacg ccgcgacgcc cggagtcaca tcct
Expressed Protein Sequence
MAHHHHHHMQ VQDVTPGVAA SGLRVDGARL WDSLMRLARI GATDKGGVCR LALTDLDRQA RDLFVAWAKD IGCTVRVDAI GNIFARRAGE RDDLPPVATG SHIDTQPTGG KFDGNYGVLA GLEVLRTLDA AGVRTRAPLE VAVWTNEEGS RFVPVMMGSG VFAGAFTLDH ALAQCDRDGV SVRDALAAIG YAGSADDGRG AHPVGAYFEA HIEQGPVLEA HDTTIGVVEG ALGQRWYDVT VHGMEAHAGP TPMELRRDAL LVAADLVRAV NGIARAHPPH GRGTVGWIDV HPNSRNVIPG RVTLTVDLRA ADDATLTAMD AQLRAACADL GAAAGMRIDI EPVVYFPPQP FDPALVEQVR AGARALGLSS MNVISGAGHD AVYLARVAPT AMIFVPCKDG ISHNEIEDAD PAHLEAGCNV LLHAMLGAAG IAGGQAR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCAAG TACAGGATGT GACTCCGGGC GTCGCGGCGT CCGGCTTGCG CGTCGATGGC GCGCGGCTGT GGGACAGCCT GATGCGGCTC GCGCGGATCG GCGCGACCGA CAAGGGCGGC GTGTGCCGCC TCGCGCTCAC GGACCTCGAT CGCCAGGCGC GCGACCTGTT CGTCGCGTGG GCGAAGGACA TCGGTTGCAC GGTGCGCGTC GATGCGATCG GCAATATCTT CGCGCGGCGC GCGGGCGAGC GGGACGACCT GCCGCCCGTG GCGACGGGCA GCCATATCGA CACGCAGCCG ACCGGCGGCA AGTTCGACGG CAACTACGGC GTGCTGGCCG GCCTCGAGGT GCTGCGTACG CTCGACGCCG CCGGGGTGCG CACGCGCGCG CCGCTCGAGG TGGCCGTCTG GACCAACGAA GAAGGGTCGC GCTTCGTCCC GGTGATGATG GGCTCCGGCG TATTCGCGGG CGCGTTCACG CTCGACCATG CGCTCGCGCA ATGCGATCGC GATGGCGTGT CGGTGCGCGA TGCGCTGGCC GCGATCGGGT ACGCGGGCAG TGCGGACGAC GGGCGCGGCG CGCATCCCGT CGGCGCGTAT TTCGAAGCGC ATATCGAGCA GGGGCCGGTG CTGGAGGCGC ACGACACGAC GATCGGCGTG GTCGAGGGCG CGCTCGGACA ACGCTGGTAC GACGTGACCG TGCACGGGAT GGAGGCGCAT GCCGGCCCCA CGCCGATGGA ACTGCGCCGC GATGCGCTGC TGGTCGCGGC CGATCTCGTG CGCGCCGTGA ACGGCATCGC GCGCGCGCAT CCGCCGCACG GACGCGGCAC GGTCGGCTGG ATCGACGTGC ACCCGAATTC GCGCAACGTG ATTCCCGGCC GCGTGACGCT GACCGTCGAC CTGCGCGCGG CCGACGACGC GACGCTGACG GCGATGGACG CGCAACTGCG CGCGGCATGC GCCGACCTGG GCGCGGCGGC CGGGATGCGG ATCGACATCG AGCCGGTCGT GTATTTTCCG CCGCAGCCGT TCGATCCGGC GCTGGTCGAG CAGGTCCGCG CCGGCGCGCG CGCGCTCGGG CTGTCGTCGA TGAACGTGAT CAGCGGCGCG GGCCACGATG CGGTGTATCT CGCGCGCGTG GCGCCGACCG CGATGATCTT CGTGCCGTGC AAGGACGGCA TCAGCCACAA CGAGATCGAA GATGCCGATC CGGCGCATCT GGAAGCCGGC TGCAACGTGC TGCTGCACGC GATGCTGGGC GCCGCCGGGA TCGCGGGGGG CCAAGCGCGA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT