BuceA.12245.c

Metallo peptidase, family M20 unassigned

CENTER ID: BuceA.12245.c
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12245.c.B1.GE37733 Full length( BuceA.12245.c ) 1 426
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.7438
RefSeq: YP_002154106.1
UniProt: B4EPC3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNAVSETVKR AAFDTSIKVD GKRLWDSLME VAKIGATPKG GVCRLALTDL DKAGRDLIVG WAKAAGCTVT VDTMGNVFMR RAGRVADAAP VVTGSHADSQ PTGGRFDGIY GVLGGLEVIR SLNDHGIETE HPVEVVIWTN EEGSRFAPAM VASGVFAGAF PLEYGLSRKD VDGKTIGEEL ARIGYAGDVP CGGRKLHAAF ELHIEQGPIL EAECKTIGVV TDAQGQRWYE ITFTGQEAHA GPTPMPRRRD ALLGASRVVD LVNRIGLDHA PFGCATVGMM QVHPNSRNVI PGRVFFTVDF RHPDDAVLAK MDAALRDGVA RIAADIGLDT ELEQIFYYKP VAFDPACVAA VRGAADRFGY SHRDIVSGAG HDACYLAQVA PTSMVFVPCV DGISHNEIED ATPAWIEAGA NVLLHAMLSR ACEPAS
NT Sequence
ATGAACGCGG TATCGGAAAC AGTGAAGCGG GCAGCTTTCG ACACGTCGAT CAAGGTCGAC GGCAAGCGGT TGTGGGACAG CCTGATGGAA GTCGCGAAAA TCGGCGCGAC GCCGAAAGGC GGCGTGTGCC GCCTCGCGCT GACCGATCTC GACAAGGCCG GTCGCGACCT GATCGTCGGC TGGGCGAAGG CGGCCGGCTG CACGGTGACG GTCGATACGA TGGGCAACGT GTTCATGCGC CGCGCGGGCC GCGTGGCCGA CGCCGCGCCG GTCGTCACGG GCTCGCACGC GGATTCGCAG CCGACCGGCG GACGCTTCGA CGGCATCTAC GGCGTGCTCG GCGGCCTCGA AGTGATTCGC AGTCTCAACG ATCACGGCAT CGAGACCGAG CATCCCGTCG AGGTCGTGAT CTGGACCAAT GAAGAGGGTT CGCGCTTCGC GCCCGCGATG GTCGCATCGG GCGTGTTCGC GGGCGCGTTC CCGCTCGAAT ACGGGCTGTC GCGCAAGGAC GTCGACGGCA AGACGATCGG CGAGGAACTG GCGCGCATCG GTTATGCGGG CGACGTGCCG TGCGGCGGGC GCAAGCTGCA CGCGGCGTTC GAGCTGCATA TCGAGCAGGG GCCGATCCTC GAAGCGGAAT GCAAGACGAT CGGCGTCGTG ACCGACGCGC AGGGGCAGCG CTGGTACGAG ATCACGTTCA CCGGCCAGGA AGCGCATGCG GGGCCGACGC CGATGCCGCG TCGCCGCGAC GCGCTGCTTG GTGCGTCGCG CGTGGTCGAT CTCGTCAACC GGATCGGCCT CGATCATGCG CCGTTCGGCT GTGCGACGGT CGGCATGATG CAGGTCCACC CGAACTCGCG CAACGTGATT CCGGGCCGCG TGTTCTTCAC CGTCGATTTC CGTCATCCGG ACGACGCCGT GCTCGCGAAG ATGGATGCGG CGCTGCGCGA CGGCGTCGCG CGCATCGCGG CCGACATCGG GCTCGACACC GAGCTCGAGC AGATTTTCTA CTACAAGCCG GTCGCGTTCG ATCCCGCGTG CGTGGCGGCC GTGCGCGGCG CGGCCGACCG CTTCGGCTAT TCGCATCGCG ACATCGTGTC GGGCGCGGGC CACGACGCGT GCTATCTCGC GCAGGTCGCG CCGACGTCGA TGGTGTTCGT GCCCTGCGTC GACGGCATCA GCCACAACGA GATCGAGGAC GCGACGCCCG CATGGATCGA GGCCGGCGCG AACGTGCTGC TGCACGCGAT GCTGTCGCGC GCATGCGAGC CGGCTTCA
Details for BuceA.12245.c.B1.GE37733
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 46kDa
OBSERVED MW: 46kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 89
Validated AA Sequence
MEVAKIGATP KGGVCRLALT DLDKAGRDLI VGWAKAAGCT VTVDTMGNVF MRRAGRVADA APVVTGSHAD SQPTGGRFDG IYGVLGGLEV IRSLNDHGIE TEHPVEVVIW TNEEGSRFAP AMVASGVFAG AFPLEYGLSR KDVDGKTIGE ELARIGYAGD VPCGGRKLHA AFELHIEQGP ILEAECKTIG VVTDAQGQRW YEITFTGQEA HAGPTPMPRR RDALLGASRV VDLVNRIGLD HAPFGCATVG MMQVHPNSRN VIPGRVFFTV DFRHPDDAVL AKMDAALRDG VARIAADIGL DTELEQIFYY KPVAFDPACV AAVRGAADRF GYSHRDIVSG AGHDACYLAQ VAPTSMVFVP CVDGISHNEI EDATPAWIEA GANVLLHA
Validated NT Sequence
tcgcgtgcag cagcacgttc gcgccggcct cgatccatgc gggcgtcgcg tcctcgatct cgttgtggct gatgccgtcg acgcagggca cgaacaccat cgacgtcggc gcgacctgcg cgagatagca cgcgtcgtgg cccgcgcccg acacgatgtc gcgatgcgaa tagccgaagc ggtcggccgc gccgcgcacg gccgccacgc acgcgggatc gaacgcgacc ggcttgtagt agaaaatctg ctcgagctcg gtgtcgagcc cgatgtcggc cgcgatgcgc gcgacgccgt cgcgcagcgc cgcatccatc ttcgcgagca cggcgtcgtc cggatgacgg aaatcgacgg tgaagaacac gcggcccgga atcacgttgc gcgagttcgg gtggacctgc atcatgccga ccgtcgcaca gccgaacggc gcatgatcga ggccgatccg gttgacgaga tcgaccacgc gcgacgcacc aagcagcgcg tcgcggcgac gcggcatcgg cgtcggcccc gcatgcgctt cctggccggt gaacgtgatc tcgtaccagc gctgcccctg cgcgtcggtc acgacgccga tcgtcttgca ttccgcttcg aggatcggcc cctgctcgat atgcagctcg aacgccgcgt gcagcttgcg cccgccgcac ggcacgtcgc ccgcataacc gatgcgcgcc agttcctcgc cgatcgtctt gccgtcgacg tccttgcgcg acagcccgta ttcgagcggg aacgcgcccg cgaacacgcc cgatgcgacc atcgcgggcg cgaagcgcga accctcttca ttggtccaga tcacgacctc gacgggatgc tcggtctcga tgccgtgatc gttgagactg cgaatcactt cgaggccgcc gagcacgccg tagatgccgt cgaagcgtcc gccggtcggc tgcgaatccg cgtgcgagcc cgtgacgacc ggcgcggcgt cggccacgcg gcccgcgcgg cgcatgaaca cgttgcccat cgtatcgacc gtcaccgtgc agccggccgc cttcgcccag ccgacgatca ggtcgcgacc ggccttgtcg agatcggtca gcgcgaggcg gcacacgccg cctttcggcg tcgcgccgat tttcgcgact tccatcaggc tgtcccacaa ccgcttgccg tcgaccttga tcgacgtgtc gaaagctgcc cgcttcactg tttccgatac cgc
Expected Protein Sequence
MAHHHHHHMN AVSETVKRAA FDTSIKVDGK RLWDSLMEVA KIGATPKGGV CRLALTDLDK AGRDLIVGWA KAAGCTVTVD TMGNVFMRRA GRVADAAPVV TGSHADSQPT GGRFDGIYGV LGGLEVIRSL NDHGIETEHP VEVVIWTNEE GSRFAPAMVA SGVFAGAFPL EYGLSRKDVD GKTIGEELAR IGYAGDVPCG GRKLHAAFEL HIEQGPILEA ECKTIGVVTD AQGQRWYEIT FTGQEAHAGP TPMPRRRDAL LGASRVVDLV NRIGLDHAPF GCATVGMMQV HPNSRNVIPG RVFFTVDFRH PDDAVLAKMD AALRDGVARI AADIGLDTEL EQIFYYKPVA FDPACVAAVR GAADRFGYSH RDIVSGAGHD ACYLAQVAPT SMVFVPCVDG ISHNEIEDAT PAWIEAGANV LLHAMLSRAC EPAS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg cggtatcgga aacagtgaag cgggcagctt tcgacacgtc gatcaaggtc gacggcaagc ggttgtggga cagcctgatg gaagtcgcga aaatcggcgc gacgccgaaa ggcggcgtgt gccgcctcgc gctgaccgat ctcgacaagg ccggtcgcga cctgatcgtc ggctgggcga aggcggccgg ctgcacggtg acggtcgata cgatgggcaa cgtgttcatg cgccgcgcgg gccgcgtggc cgacgccgcg ccggtcgtca cgggctcgca cgcggattcg cagccgaccg gcggacgctt cgacggcatc tacggcgtgc tcggcggcct cgaagtgatt cgcagtctca acgatcacgg catcgagacc gagcatcccg tcgaggtcgt gatctggacc aatgaagagg gttcgcgctt cgcgcccgcg atggtcgcat cgggcgtgtt cgcgggcgcg ttcccgctcg aatacgggct gtcgcgcaag gacgtcgacg gcaagacgat cggcgaggaa ctggcgcgca tcggttatgc gggcgacgtg ccgtgcggcg ggcgcaagct gcacgcggcg ttcgagctgc atatcgagca ggggccgatc ctcgaagcgg aatgcaagac gatcggcgtc gtgaccgacg cgcaggggca gcgctggtac gagatcacgt tcaccggcca ggaagcgcat gcggggccga cgccgatgcc gcgtcgccgc gacgcgctgc ttggtgcgtc gcgcgtggtc gatctcgtca accggatcgg cctcgatcat gcgccgttcg gctgtgcgac ggtcggcatg atgcaggtcc acccgaactc gcgcaacgtg attccgggcc gcgtgttctt caccgtcgat ttccgtcatc cggacgacgc cgtgctcgcg aagatggatg cggcgctgcg cgacggcgtc gcgcgcatcg cggccgacat cgggctcgac accgagctcg agcagatttt ctactacaag ccggtcgcgt tcgatcccgc gtgcgtggcg gccgtgcgcg gcgcggccga ccgcttcggc tattcgcatc gcgacatcgt gtcgggcgcg ggccacgacg cgtgctatct cgcgcaggtc gcgccgacgt cgatggtgtt cgtgccctgc gtcgacggca tcagccacaa cgagatcgag gacgcgacgc ccgcatggat cgaggccggc gcgaacgtgc tgctgcacgc gatgctgtcg cgcgcatgcg agccggcttc atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat