BuceA.12245.b
Putative amidohydrolase/peptidase
| CENTER ID: | BuceA.12245.b |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.12245.b.B1.GE37732 | Full length( BuceA.12245.b ) | 1 | 416 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.12245.b.B1.PS02058 | Full length( BuceA.12245.b ) | 1 | 416 |
Structures
4WJB
DEPOSITED: 9/29/2014
DETERMINATION: XRay
CLONE: BuceA.12245.b.B1.GE37732
PROTEIN: BuceA.12245.b.B1.PS02058
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216591.5.peg.4950 |
| RefSeq: | YP_002233837.1 |
| UniProt: | B4EHA1 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRDAPASMPR VDGDRLWASL ERMAQIGATP KGGVCRLALT DLDRESRDLF VQWAREAGCT VRVDRMGNVF ARRAGRRPDA APVMTGSHAD SQPTGGRYDG IYGVLGGLEV VRALNDAAIE TERPVDVVIW TNEEGSRFAP AMVSAGVFSG VYTLEYGLSR TDGAGRTIGE ELERIGYAGA EPVGGYPVHA AYELHIEQGA ILERAGKTIG VVTAGQGQRW YEVTLTGVDA HAGTTPMAFR RDALVGAARM ISFVEVLGRR YAPDARATVG MIEARPNSRN TVPGGCFFTV EFRHPDDAVL DELDAALRAE LARVADETGL GAQIEQIFTY APVPFAPRCI DTVRDAARSL GLSHMDIVSG AGHDACYVAR VAPTGMIFVP CVDGLSHNEA EAITPEWATA GADVLLRAVL QSAQEA
NT Sequence
ATGCGTGACG CTCCCGCAAG CATGCCGCGC GTCGACGGCG ACCGCCTGTG GGCGTCGCTC GAACGGATGG CGCAGATCGG CGCGACGCCG AAGGGCGGCG TGTGCCGTCT CGCGCTGACC GACCTCGACC GCGAGTCGCG CGACCTGTTC GTGCAGTGGG CGCGTGAAGC CGGCTGCACG GTGCGCGTCG ATCGGATGGG CAACGTGTTC GCGCGCCGCG CCGGTCGCCG TCCCGACGCC GCACCGGTGA TGACGGGCTC GCATGCCGAT TCCCAGCCGA CCGGCGGGCG TTACGACGGC ATCTACGGCG TGCTCGGCGG GCTCGAAGTG GTGCGTGCGC TGAACGACGC GGCCATCGAG ACCGAGCGTC CGGTCGACGT CGTGATCTGG ACCAACGAGG AAGGCTCGCG CTTCGCGCCG GCGATGGTGT CGGCCGGCGT GTTCTCGGGC GTCTATACGC TCGAATATGG GCTGTCGCGC ACCGACGGCG CGGGCCGGAC GATCGGCGAG GAGCTCGAAC GGATCGGCTA CGCGGGCGCG GAGCCGGTCG GCGGCTATCC GGTGCACGCG GCATATGAGC TGCACATCGA GCAGGGCGCG ATTCTCGAAC GGGCCGGCAA GACGATCGGC GTCGTGACGG CCGGGCAGGG GCAGCGCTGG TACGAGGTGA CGCTCACCGG TGTCGACGCG CACGCGGGCA CGACGCCGAT GGCGTTCCGC CGCGACGCGC TGGTCGGCGC GGCGCGGATG ATCTCGTTCG TCGAAGTGCT CGGCCGCCGC TACGCACCGG ATGCGCGCGC AACGGTCGGG ATGATCGAGG CGCGGCCGAA TTCGCGCAAC ACGGTGCCGG GCGGCTGCTT TTTCACGGTG GAATTCCGGC ATCCCGACGA TGCGGTGCTC GACGAACTGG ACGCGGCGTT GCGCGCGGAG CTCGCGCGCG TGGCCGACGA AACCGGCCTC GGCGCGCAGA TCGAGCAGAT CTTCACGTAT GCGCCGGTGC CGTTCGCGCC ACGCTGCATC GACACGGTGC GCGACGCGGC GCGCTCGCTC GGGCTGTCGC ACATGGACAT CGTGTCCGGC GCGGGCCACG ACGCGTGCTA TGTCGCGCGT GTCGCGCCGA CCGGCATGAT CTTCGTGCCG TGCGTCGACG GGCTGAGCCA CAACGAGGCC GAAGCGATTA CGCCCGAATG GGCGACGGCC GGCGCCGACG TGCTGTTGCG CGCGGTGCTG CAGAGCGCGC AGGAAGCG
Details for BuceA.12245.b.B1.GE37732
| HARVESTED ON: | 2/5/2014 |
| SEQUENCED ON: | 2/6/2014 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Validated AA Sequence
MPRVDGDRLW ASLERMAQIG ATPKGGVCRL ALTDLDRESR DLFVQWAREA GCTVRVDRMG NVFARRAGRR PDAAPVMTGS HADSQPTGGR YDGIYGVLGG LEVVRALNDA AIETERPVDV VIWTNEEGSR FAPAMVSAGV FSGVYTLEYG LSRTDGAGRT IGEELERIGY AGAEPVGGYP VHAAYELHIE QGAILERAGK TIGVVTAGQG QRWYEVTLTG VDAHAGTTPM AFRRDALVGA ARMISFVEVL GRRYAPDARA TVGMIEARPN SRNTVPGGCF FTVEFRHPDD AVLDELDAAL RAELARVADE TGLGAQIEQI FTYAPVPFAP RCIDTVRDAA RSLGLSHMDI VSGAGHDACY VARVAPTGMI FVPCVDGLSH NEAEAITPEW ATAGADVLL
Validated NT Sequence
cgcaacagca cgtcggcgcc ggccgtcgcc cattcgggcg taatcgcttc ggcctcgttg tggctcagcc cgtcgacgca cggcacgaag atcatgccgg tcggcgcgac acgcgcgaca tagcacgcgt cgtggcccgc gccggacacg atgtccatgt gcgacagccc gagcgagcgc gccgcgtcgc gcaccgtgtc gatgcagcgt ggcgcgaacg gcaccggcgc atacgtgaag atctgctcga tctgcgcgcc gaggccggtt tcgtcggcca cgcgcgcgag ctccgcgcgc aacgccgcgt ccagttcgtc gagcaccgca tcgtcgggat gccggaattc caccgtgaaa aagcagccgc ccggcaccgt gttgcgcgaa ttcggccgcg cctcgatcat cccgaccgtt gcgcgcgcat ccggtgcgta gcggcggccg agcacttcga cgaacgagat catccgcgcc gcgccgacca gcgcgtcgcg gcggaacgcc atcggcgtcg tgcccgcgtg cgcgtcgaca ccggtgagcg tcacctcgta ccagcgctgc ccctgcccgg ccgtcacgac gccgatcgtc ttgccggccc gttcgagaat cgcgccctgc tcgatgtgca gctcatatgc cgcgtgcacc ggatagccgc cgaccggctc cgcgcccgcg tagccgatcc gttcgagctc ctcgccgatc gtccggcccg cgccgtcggt gcgcgacagc ccatattcga gcgtatagac gcccgagaac acgccggccg acaccatcgc cggcgcgaag cgcgagcctt cctcgttggt ccagatcacg acgtcgaccg gacgctcggt ctcgatggcc gcgtcgttca gcgcacgcac cacttcgagc ccgccgagca cgccgtagat gccgtcgtaa cgcccgccgg tcggctggga atcggcatgc gagcccgtca tcaccggtgc ggcgtcggga cggcgaccgg cgcggcgcgc gaacacgttg cccatccgat cgacgcgcac cgtgcagccg gcttcacgcg cccactgcac gaacaggtcg cgcgactcgc ggtcgaggtc ggtcagcgcg agacggcaca cgccgccctt cggcgtcgcg ccgatctgcg ccatccgttc gagcgacgcc cacaggcggt cgccgtcgac gcgcggcatg cttgcgggag cgtc
Expected Protein Sequence
MAHHHHHHMR DAPASMPRVD GDRLWASLER MAQIGATPKG GVCRLALTDL DRESRDLFVQ WAREAGCTVR VDRMGNVFAR RAGRRPDAAP VMTGSHADSQ PTGGRYDGIY GVLGGLEVVR ALNDAAIETE RPVDVVIWTN EEGSRFAPAM VSAGVFSGVY TLEYGLSRTD GAGRTIGEEL ERIGYAGAEP VGGYPVHAAY ELHIEQGAIL ERAGKTIGVV TAGQGQRWYE VTLTGVDAHA GTTPMAFRRD ALVGAARMIS FVEVLGRRYA PDARATVGMI EARPNSRNTV PGGCFFTVEF RHPDDAVLDE LDAALRAELA RVADETGLGA QIEQIFTYAP VPFAPRCIDT VRDAARSLGL SHMDIVSGAG HDACYVARVA PTGMIFVPCV DGLSHNEAEA ITPEWATAGA DVLLRAVLQS AQEA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgtg acgctcccgc aagcatgccg cgcgtcgacg gcgaccgcct gtgggcgtcg ctcgaacgga tggcgcagat cggcgcgacg ccgaagggcg gcgtgtgccg tctcgcgctg accgacctcg accgcgagtc gcgcgacctg ttcgtgcagt gggcgcgtga agccggctgc acggtgcgcg tcgatcggat gggcaacgtg ttcgcgcgcc gcgccggtcg ccgtcccgac gccgcaccgg tgatgacggg ctcgcatgcc gattcccagc cgaccggcgg gcgttacgac ggcatctacg gcgtgctcgg cgggctcgaa gtggtgcgtg cgctgaacga cgcggccatc gagaccgagc gtccggtcga cgtcgtgatc tggaccaacg aggaaggctc gcgcttcgcg ccggcgatgg tgtcggccgg cgtgttctcg ggcgtctata cgctcgaata tgggctgtcg cgcaccgacg gcgcgggccg gacgatcggc gaggagctcg aacggatcgg ctacgcgggc gcggagccgg tcggcggcta tccggtgcac gcggcatatg agctgcacat cgagcagggc gcgattctcg aacgggccgg caagacgatc ggcgtcgtga cggccgggca ggggcagcgc tggtacgagg tgacgctcac cggtgtcgac gcgcacgcgg gcacgacgcc gatggcgttc cgccgcgacg cgctggtcgg cgcggcgcgg atgatctcgt tcgtcgaagt gctcggccgc cgctacgcac cggatgcgcg cgcaacggtc gggatgatcg aggcgcggcc gaattcgcgc aacacggtgc cgggcggctg ctttttcacg gtggaattcc ggcatcccga cgatgcggtg ctcgacgaac tggacgcggc gttgcgcgcg gagctcgcgc gcgtggccga cgaaaccggc ctcggcgcgc agatcgagca gatcttcacg tatgcgccgg tgccgttcgc gccacgctgc atcgacacgg tgcgcgacgc ggcgcgctcg ctcgggctgt cgcacatgga catcgtgtcc ggcgcgggcc acgacgcgtg ctatgtcgcg cgtgtcgcgc cgaccggcat gatcttcgtg ccgtgcgtcg acgggctgag ccacaacgag gccgaagcga ttacgcccga atgggcgacg gccggcgccg acgtgctgtt gcgcgcggtg ctgcagagcg cgcaggaagc gtgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuceA.12245.b.B1.PS02058
| PURIFICATION DATe: | 5/21/2014 |
| CONCENTRATION: | 53.48mg/ml |
| OBSERVED MW: | 45kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 94 |
Protocol Notes
notes unavailable
Validated AA Sequence
MPRVDGDRLW ASLERMAQIG ATPKGGVCRL ALTDLDRESR DLFVQWAREA GCTVRVDRMG NVFARRAGRR PDAAPVMTGS HADSQPTGGR YDGIYGVLGG LEVVRALNDA AIETERPVDV VIWTNEEGSR FAPAMVSAGV FSGVYTLEYG LSRTDGAGRT IGEELERIGY AGAEPVGGYP VHAAYELHIE QGAILERAGK TIGVVTAGQG QRWYEVTLTG VDAHAGTTPM AFRRDALVGA ARMISFVEVL GRRYAPDARA TVGMIEARPN SRNTVPGGCF FTVEFRHPDD AVLDELDAAL RAELARVADE TGLGAQIEQI FTYAPVPFAP RCIDTVRDAA RSLGLSHMDI VSGAGHDACY VARVAPTGMI FVPCVDGLSH NEAEAITPEW ATAGADVLL
Validated NT Sequence
cgcaacagca cgtcggcgcc ggccgtcgcc cattcgggcg taatcgcttc ggcctcgttg tggctcagcc cgtcgacgca cggcacgaag atcatgccgg tcggcgcgac acgcgcgaca tagcacgcgt cgtggcccgc gccggacacg atgtccatgt gcgacagccc gagcgagcgc gccgcgtcgc gcaccgtgtc gatgcagcgt ggcgcgaacg gcaccggcgc atacgtgaag atctgctcga tctgcgcgcc gaggccggtt tcgtcggcca cgcgcgcgag ctccgcgcgc aacgccgcgt ccagttcgtc gagcaccgca tcgtcgggat gccggaattc caccgtgaaa aagcagccgc ccggcaccgt gttgcgcgaa ttcggccgcg cctcgatcat cccgaccgtt gcgcgcgcat ccggtgcgta gcggcggccg agcacttcga cgaacgagat catccgcgcc gcgccgacca gcgcgtcgcg gcggaacgcc atcggcgtcg tgcccgcgtg cgcgtcgaca ccggtgagcg tcacctcgta ccagcgctgc ccctgcccgg ccgtcacgac gccgatcgtc ttgccggccc gttcgagaat cgcgccctgc tcgatgtgca gctcatatgc cgcgtgcacc ggatagccgc cgaccggctc cgcgcccgcg tagccgatcc gttcgagctc ctcgccgatc gtccggcccg cgccgtcggt gcgcgacagc ccatattcga gcgtatagac gcccgagaac acgccggccg acaccatcgc cggcgcgaag cgcgagcctt cctcgttggt ccagatcacg acgtcgaccg gacgctcggt ctcgatggcc gcgtcgttca gcgcacgcac cacttcgagc ccgccgagca cgccgtagat gccgtcgtaa cgcccgccgg tcggctggga atcggcatgc gagcccgtca tcaccggtgc ggcgtcggga cggcgaccgg cgcggcgcgc gaacacgttg cccatccgat cgacgcgcac cgtgcagccg gcttcacgcg cccactgcac gaacaggtcg cgcgactcgc ggtcgaggtc ggtcagcgcg agacggcaca cgccgccctt cggcgtcgcg ccgatctgcg ccatccgttc gagcgacgcc cacaggcggt cgccgtcgac gcgcggcatg cttgcgggag cgtc
Expressed Protein Sequence
MAHHHHHHMR DAPASMPRVD GDRLWASLER MAQIGATPKG GVCRLALTDL DRESRDLFVQ WAREAGCTVR VDRMGNVFAR RAGRRPDAAP VMTGSHADSQ PTGGRYDGIY GVLGGLEVVR ALNDAAIETE RPVDVVIWTN EEGSRFAPAM VSAGVFSGVY TLEYGLSRTD GAGRTIGEEL ERIGYAGAEP VGGYPVHAAY ELHIEQGAIL ERAGKTIGVV TAGQGQRWYE VTLTGVDAHA GTTPMAFRRD ALVGAARMIS FVEVLGRRYA PDARATVGMI EARPNSRNTV PGGCFFTVEF RHPDDAVLDE LDAALRAELA RVADETGLGA QIEQIFTYAP VPFAPRCIDT VRDAARSLGL SHMDIVSGAG HDACYVARVA PTGMIFVPCV DGLSHNEAEA ITPEWATAGA DVLLRAVLQS AQEA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCGTG ACGCTCCCGC AAGCATGCCG CGCGTCGACG GCGACCGCCT GTGGGCGTCG CTCGAACGGA TGGCGCAGAT CGGCGCGACG CCGAAGGGCG GCGTGTGCCG TCTCGCGCTG ACCGACCTCG ACCGCGAGTC GCGCGACCTG TTCGTGCAGT GGGCGCGTGA AGCCGGCTGC ACGGTGCGCG TCGATCGGAT GGGCAACGTG TTCGCGCGCC GCGCCGGTCG CCGTCCCGAC GCCGCACCGG TGATGACGGG CTCGCATGCC GATTCCCAGC CGACCGGCGG GCGTTACGAC GGCATCTACG GCGTGCTCGG CGGGCTCGAA GTGGTGCGTG CGCTGAACGA CGCGGCCATC GAGACCGAGC GTCCGGTCGA CGTCGTGATC TGGACCAACG AGGAAGGCTC GCGCTTCGCG CCGGCGATGG TGTCGGCCGG CGTGTTCTCG GGCGTCTATA CGCTCGAATA TGGGCTGTCG CGCACCGACG GCGCGGGCCG GACGATCGGC GAGGAGCTCG AACGGATCGG CTACGCGGGC GCGGAGCCGG TCGGCGGCTA TCCGGTGCAC GCGGCATATG AGCTGCACAT CGAGCAGGGC GCGATTCTCG AACGGGCCGG CAAGACGATC GGCGTCGTGA CGGCCGGGCA GGGGCAGCGC TGGTACGAGG TGACGCTCAC CGGTGTCGAC GCGCACGCGG GCACGACGCC GATGGCGTTC CGCCGCGACG CGCTGGTCGG CGCGGCGCGG ATGATCTCGT TCGTCGAAGT GCTCGGCCGC CGCTACGCAC CGGATGCGCG CGCAACGGTC GGGATGATCG AGGCGCGGCC GAATTCGCGC AACACGGTGC CGGGCGGCTG CTTTTTCACG GTGGAATTCC GGCATCCCGA CGATGCGGTG CTCGACGAAC TGGACGCGGC GTTGCGCGCG GAGCTCGCGC GCGTGGCCGA CGAAACCGGC CTCGGCGCGC AGATCGAGCA GATCTTCACG TATGCGCCGG TGCCGTTCGC GCCACGCTGC ATCGACACGG TGCGCGACGC GGCGCGCTCG CTCGGGCTGT CGCACATGGA CATCGTGTCC GGCGCGGGCC ACGACGCGTG CTATGTCGCG CGTGTCGCGC CGACCGGCAT GATCTTCGTG CCGTGCGTCG ACGGGCTGAG CCACAACGAG GCCGAAGCGA TTACGCCCGA ATGGGCGACG GCCGGCGCCG ACGTGCTGTT GCGCGCGGTG CTGCAGAGCG CGCAGGAAGC GTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT