BuceA.12245.a

Metallo peptidase, family M20 unassigned

CENTER ID: BuceA.12245.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12245.a.B1.GE37731 Full length( BuceA.12245.a ) 1 425
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.12245.a.B1.PS02038 Full length( BuceA.12245.a ) 1 425

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1268
RefSeq: YP_002230362.1
UniProt: B4EEG1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHMNDLLEID GARLWQSLAD MARVGATPRG GVRRLALTDD DRRGRDLFAQ WCRDAGMTVS VDTVGNLFAR RDGADAHAAP VLIGSHLDTQ PEGGRFDGVY GVLAALELVR TLNDAGIGTD KPLEIVSWTN EEGARFAPAM LGSAVFTGAL PLDDALAKQD ADGVTLGAAL DACGYRGTRA TGGAVDAYFE AHIEQGPVLE ANGTTIGIVT GGQAIRWLDV TVTGVAAHAG TTPMPYRKDA YFASAQIALE LERIVAAYAP RGLATIGQVG IRNASRNTIA GDLTFTVDLR HHDDAEVDAM ERDLRDACAR VAAARGVQVA VENCWRSPAT PFDRACVGLV AQAAAAFGYT HERIVSGAGH DAILLARRFP TAMVFIPCVD GLSHNEAEDA LPDDVTRGTN VLLHAVLARA GIAARVDAAA AAHGA
NT Sequence
ATGCATATGA ACGATCTGCT GGAAATCGAC GGCGCCCGCC TGTGGCAGAG CCTGGCCGAC ATGGCGCGCG TCGGCGCGAC GCCGCGCGGC GGCGTGCGGC GTCTCGCCCT CACCGATGAC GACCGGCGCG GGCGCGACCT GTTCGCGCAA TGGTGCCGCG ACGCGGGGAT GACGGTGAGC GTCGACACGG TCGGCAACCT GTTCGCCCGG CGTGACGGCG CTGATGCGCA CGCCGCGCCG GTGCTGATCG GCAGCCATCT CGACACGCAG CCCGAAGGCG GCCGCTTCGA CGGCGTCTAT GGCGTGCTCG CCGCGCTCGA ACTCGTGCGC ACGCTGAACG ACGCCGGCAT CGGGACCGAC AAGCCGCTCG AGATCGTGTC GTGGACCAAC GAGGAAGGGG CGCGCTTCGC GCCCGCGATG CTCGGTTCGG CGGTGTTCAC GGGCGCGTTG CCGCTCGACG ACGCGCTCGC GAAGCAGGAC GCCGACGGCG TCACGCTCGG CGCGGCGCTC GACGCGTGCG GCTATCGCGG CACGCGTGCG ACGGGCGGTG CGGTCGACGC GTATTTCGAG GCGCATATCG AACAGGGCCC CGTGCTCGAG GCGAACGGCA CGACGATCGG CATCGTCACG GGCGGCCAGG CGATCCGCTG GCTCGACGTG ACGGTGACGG GCGTGGCCGC GCACGCGGGC ACGACGCCGA TGCCGTATCG CAAGGACGCG TATTTCGCGA GCGCGCAGAT CGCGCTCGAA CTCGAACGGA TCGTCGCCGC TTATGCGCCG CGCGGGCTCG CGACGATCGG CCAGGTCGGC ATCCGCAATG CGTCGCGCAA CACGATTGCC GGCGACCTGA CGTTCACGGT CGACCTGCGT CATCACGACG ATGCGGAAGT CGATGCGATG GAGCGCGACC TGCGCGACGC CTGCGCACGC GTCGCCGCCG CGCGCGGCGT GCAGGTGGCG GTCGAAAACT GCTGGCGCAG CCCGGCGACG CCGTTCGACC GCGCTTGCGT CGGGCTGGTC GCGCAGGCGG CGGCCGCATT CGGCTACACG CACGAACGGA TCGTGAGCGG CGCCGGCCAC GACGCGATCC TGCTCGCACG CCGCTTCCCG ACCGCGATGG TGTTCATTCC GTGCGTCGAC GGCCTGTCGC ACAACGAAGC CGAGGATGCG TTGCCCGACG ACGTGACGCG CGGCACGAAC GTGCTGCTCC ATGCGGTGCT GGCGCGGGCC GGCATCGCGG CGCGCGTCGA CGCGGCGGCT GCCGCGCACG GCGCC
Details for BuceA.12245.a.B1.GE37731
HARVESTED ON: 2/5/2014
SEQUENCED ON: 2/6/2014
EXPECTED MW: 46kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 26
PERCENT COVERAGE: 92
Validated AA Sequence
MEQHVRAARH VVGQRILGFV VRQAVDARNE HHRGREAACE QDRVVAGAAH DPFVRVAECG RRLRDQPDAS AVERRRRAAP AVFDRHLHAA RGGDACAGVA QVALHRIDFR IVVMTQVDRE RQVAGNRVAR RIADADLADR REPARRISGD DPFEFERDLR AREIRVLAIR HRRRARVRGH ARHRHVEPAD RLAARDDADR RAVRLEHGAL FDMRLEIRVD RTARRTRAAI AARVERRAER DAVGVLLRER VVERQRAREH RRTEHRGREA RPFLVGPRHD LERLVGPDAG VVQRAHEFER GEHAIDAVEA AAFGLRVEMA ADQHRRGVRI SAVTPGEQVA DRVDAHRHPR VAAPLREQVA PAPVVIGEGE TPHAAARRRA DARHVGQALP QAGAVDFQQI V
Validated NT Sequence
cgacgcgcgc cgcgatgccg gcccgcgcca gcaccgcatg gagcagcacg ttcgtgccgc gcgtcacgtc gtcgggcaac gcatcctcgg cttcgttgtg cgacaggccg tcgacgcacg gaatgaacac catcgcggtc gggaagcggc gtgcgagcag gatcgcgtcg tggccggcgc cgctcacgat ccgttcgtgc gtgtagccga atgcggccgc cgcctgcgcg accagcccga cgcaagcgcg gtcgaacggc gtcgccgggc tgcgccagca gttttcgacc gccacctgca cgccgcgcgc ggcggcgacg cgtgcgcagg cgtcgcgcag gtcgcgctcc atcgcatcga cttccgcatc gtcgtgatga cgcaggtcga ccgtgaacgt caggtcgccg gcaatcgtgt tgcgcgacgc attgcggatg ccgacctggc cgatcgtcgc gagcccgcgc ggcgcataag cggcgacgat ccgttcgagt tcgagcgcga tctgcgcgct cgcgaaatac gcgtccttgc gatacggcat cggcgtcgtg cccgcgtgcg cggccacgcc cgtcaccgtc acgtcgagcc agcggatcgc ctggccgccc gtgacgatgc cgatcgtcgt gccgttcgcc tcgagcacgg ggccctgttc gatatgcgcc tcgaaatacg cgtcgaccgc accgcccgtc gcacgcgtgc cgcgatagcc gcacgcgtcg agcgccgcgc cgagcgtgac gccgtcggcg tcctgcttcg cgagcgcgtc gtcgagcggc aacgcgcccg tgaacaccgc cgaaccgagc atcgcgggcg cgaagcgcgc cccttcctcg ttggtccacg acacgatctc gagcggcttg tcggtcccga tgccggcgtc gttcagcgtg cgcacgagtt cgagcgcggc gagcacgcca tagacgccgt cgaagcggcc gccttcgggc tgcgtgtcga gatggctgcc gatcagcacc ggcgcggcgt gcgcatcagc gccgtcacgc cgggcgaaca ggttgccgac cgtgtcgacg ctcaccgtca tccccgcgtc gcggcaccat tgcgcgaaca ggtcgcgccc gcgccggtcg tcatcggtga gggcgagacg ccgcacgccg ccgcgcggcg tcgcgccgac gcgcgccatg tcggccaggc tctgccacag gcgggcgccg tcgatttcca gcagatcgtt c
Expected Protein Sequence
MAHHHHHHMH MNDLLEIDGA RLWQSLADMA RVGATPRGGV RRLALTDDDR RGRDLFAQWC RDAGMTVSVD TVGNLFARRD GADAHAAPVL IGSHLDTQPE GGRFDGVYGV LAALELVRTL NDAGIGTDKP LEIVSWTNEE GARFAPAMLG SAVFTGALPL DDALAKQDAD GVTLGAALDA CGYRGTRATG GAVDAYFEAH IEQGPVLEAN GTTIGIVTGG QAIRWLDVTV TGVAAHAGTT PMPYRKDAYF ASAQIALELE RIVAAYAPRG LATIGQVGIR NASRNTIAGD LTFTVDLRHH DDAEVDAMER DLRDACARVA AARGVQVAVE NCWRSPATPF DRACVGLVAQ AAAAFGYTHE RIVSGAGHDA ILLARRFPTA MVFIPCVDGL SHNEAEDALP DDVTRGTNVL LHAVLARAGI AARVDAAAAA HGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcata tgaacgatct gctggaaatc gacggcgccc gcctgtggca gagcctggcc gacatggcgc gcgtcggcgc gacgccgcgc ggcggcgtgc ggcgtctcgc cctcaccgat gacgaccggc gcgggcgcga cctgttcgcg caatggtgcc gcgacgcggg gatgacggtg agcgtcgaca cggtcggcaa cctgttcgcc cggcgtgacg gcgctgatgc gcacgccgcg ccggtgctga tcggcagcca tctcgacacg cagcccgaag gcggccgctt cgacggcgtc tatggcgtgc tcgccgcgct cgaactcgtg cgcacgctga acgacgccgg catcgggacc gacaagccgc tcgagatcgt gtcgtggacc aacgaggaag gggcgcgctt cgcgcccgcg atgctcggtt cggcggtgtt cacgggcgcg ttgccgctcg acgacgcgct cgcgaagcag gacgccgacg gcgtcacgct cggcgcggcg ctcgacgcgt gcggctatcg cggcacgcgt gcgacgggcg gtgcggtcga cgcgtatttc gaggcgcata tcgaacaggg ccccgtgctc gaggcgaacg gcacgacgat cggcatcgtc acgggcggcc aggcgatccg ctggctcgac gtgacggtga cgggcgtggc cgcgcacgcg ggcacgacgc cgatgccgta tcgcaaggac gcgtatttcg cgagcgcgca gatcgcgctc gaactcgaac ggatcgtcgc cgcttatgcg ccgcgcgggc tcgcgacgat cggccaggtc ggcatccgca atgcgtcgcg caacacgatt gccggcgacc tgacgttcac ggtcgacctg cgtcatcacg acgatgcgga agtcgatgcg atggagcgcg acctgcgcga cgcctgcgca cgcgtcgccg ccgcgcgcgg cgtgcaggtg gcggtcgaaa actgctggcg cagcccggcg acgccgttcg accgcgcttg cgtcgggctg gtcgcgcagg cggcggccgc attcggctac acgcacgaac ggatcgtgag cggcgccggc cacgacgcga tcctgctcgc acgccgcttc ccgaccgcga tggtgttcat tccgtgcgtc gacggcctgt cgcacaacga agccgaggat gcgttgcccg acgacgtgac gcgcggcacg aacgtgctgc tccatgcggt gctggcgcgg gccggcatcg cggcgcgcgt cgacgcggcg gctgccgcgc acggcgcctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuceA.12245.a.B1.PS02038
PURIFICATION DATe: 4/9/2014
CONCENTRATION: 29.6mg/ml
OBSERVED MW: 47kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 2
VIAL VOLUME: 110µl
PERCENT IDENTITY: 26
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MEQHVRAARH VVGQRILGFV VRQAVDARNE HHRGREAACE QDRVVAGAAH DPFVRVAECG RRLRDQPDAS AVERRRRAAP AVFDRHLHAA RGGDACAGVA QVALHRIDFR IVVMTQVDRE RQVAGNRVAR RIADADLADR REPARRISGD DPFEFERDLR AREIRVLAIR HRRRARVRGH ARHRHVEPAD RLAARDDADR RAVRLEHGAL FDMRLEIRVD RTARRTRAAI AARVERRAER DAVGVLLRER VVERQRAREH RRTEHRGREA RPFLVGPRHD LERLVGPDAG VVQRAHEFER GEHAIDAVEA AAFGLRVEMA ADQHRRGVRI SAVTPGEQVA DRVDAHRHPR VAAPLREQVA PAPVVIGEGE TPHAAARRRA DARHVGQALP QAGAVDFQQI V
Validated NT Sequence
cgacgcgcgc cgcgatgccg gcccgcgcca gcaccgcatg gagcagcacg ttcgtgccgc gcgtcacgtc gtcgggcaac gcatcctcgg cttcgttgtg cgacaggccg tcgacgcacg gaatgaacac catcgcggtc gggaagcggc gtgcgagcag gatcgcgtcg tggccggcgc cgctcacgat ccgttcgtgc gtgtagccga atgcggccgc cgcctgcgcg accagcccga cgcaagcgcg gtcgaacggc gtcgccgggc tgcgccagca gttttcgacc gccacctgca cgccgcgcgc ggcggcgacg cgtgcgcagg cgtcgcgcag gtcgcgctcc atcgcatcga cttccgcatc gtcgtgatga cgcaggtcga ccgtgaacgt caggtcgccg gcaatcgtgt tgcgcgacgc attgcggatg ccgacctggc cgatcgtcgc gagcccgcgc ggcgcataag cggcgacgat ccgttcgagt tcgagcgcga tctgcgcgct cgcgaaatac gcgtccttgc gatacggcat cggcgtcgtg cccgcgtgcg cggccacgcc cgtcaccgtc acgtcgagcc agcggatcgc ctggccgccc gtgacgatgc cgatcgtcgt gccgttcgcc tcgagcacgg ggccctgttc gatatgcgcc tcgaaatacg cgtcgaccgc accgcccgtc gcacgcgtgc cgcgatagcc gcacgcgtcg agcgccgcgc cgagcgtgac gccgtcggcg tcctgcttcg cgagcgcgtc gtcgagcggc aacgcgcccg tgaacaccgc cgaaccgagc atcgcgggcg cgaagcgcgc cccttcctcg ttggtccacg acacgatctc gagcggcttg tcggtcccga tgccggcgtc gttcagcgtg cgcacgagtt cgagcgcggc gagcacgcca tagacgccgt cgaagcggcc gccttcgggc tgcgtgtcga gatggctgcc gatcagcacc ggcgcggcgt gcgcatcagc gccgtcacgc cgggcgaaca ggttgccgac cgtgtcgacg ctcaccgtca tccccgcgtc gcggcaccat tgcgcgaaca ggtcgcgccc gcgccggtcg tcatcggtga gggcgagacg ccgcacgccg ccgcgcggcg tcgcgccgac gcgcgccatg tcggccaggc tctgccacag gcgggcgccg tcgatttcca gcagatcgtt c
Expressed Protein Sequence
MAHHHHHHMH MNDLLEIDGA RLWQSLADMA RVGATPRGGV RRLALTDDDR RGRDLFAQWC RDAGMTVSVD TVGNLFARRD GADAHAAPVL IGSHLDTQPE GGRFDGVYGV LAALELVRTL NDAGIGTDKP LEIVSWTNEE GARFAPAMLG SAVFTGALPL DDALAKQDAD GVTLGAALDA CGYRGTRATG GAVDAYFEAH IEQGPVLEAN GTTIGIVTGG QAIRWLDVTV TGVAAHAGTT PMPYRKDAYF ASAQIALELE RIVAAYAPRG LATIGQVGIR NASRNTIAGD LTFTVDLRHH DDAEVDAMER DLRDACARVA AARGVQVAVE NCWRSPATPF DRACVGLVAQ AAAAFGYTHE RIVSGAGHDA ILLARRFPTA MVFIPCVDGL SHNEAEDALP DDVTRGTNVL LHAVLARAGI AARVDAAAAA HGA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCATA TGAACGATCT GCTGGAAATC GACGGCGCCC GCCTGTGGCA GAGCCTGGCC GACATGGCGC GCGTCGGCGC GACGCCGCGC GGCGGCGTGC GGCGTCTCGC CCTCACCGAT GACGACCGGC GCGGGCGCGA CCTGTTCGCG CAATGGTGCC GCGACGCGGG GATGACGGTG AGCGTCGACA CGGTCGGCAA CCTGTTCGCC CGGCGTGACG GCGCTGATGC GCACGCCGCG CCGGTGCTGA TCGGCAGCCA TCTCGACACG CAGCCCGAAG GCGGCCGCTT CGACGGCGTC TATGGCGTGC TCGCCGCGCT CGAACTCGTG CGCACGCTGA ACGACGCCGG CATCGGGACC GACAAGCCGC TCGAGATCGT GTCGTGGACC AACGAGGAAG GGGCGCGCTT CGCGCCCGCG ATGCTCGGTT CGGCGGTGTT CACGGGCGCG TTGCCGCTCG ACGACGCGCT CGCGAAGCAG GACGCCGACG GCGTCACGCT CGGCGCGGCG CTCGACGCGT GCGGCTATCG CGGCACGCGT GCGACGGGCG GTGCGGTCGA CGCGTATTTC GAGGCGCATA TCGAACAGGG CCCCGTGCTC GAGGCGAACG GCACGACGAT CGGCATCGTC ACGGGCGGCC AGGCGATCCG CTGGCTCGAC GTGACGGTGA CGGGCGTGGC CGCGCACGCG GGCACGACGC CGATGCCGTA TCGCAAGGAC GCGTATTTCG CGAGCGCGCA GATCGCGCTC GAACTCGAAC GGATCGTCGC CGCTTATGCG CCGCGCGGGC TCGCGACGAT CGGCCAGGTC GGCATCCGCA ATGCGTCGCG CAACACGATT GCCGGCGACC TGACGTTCAC GGTCGACCTG CGTCATCACG ACGATGCGGA AGTCGATGCG ATGGAGCGCG ACCTGCGCGA CGCCTGCGCA CGCGTCGCCG CCGCGCGCGG CGTGCAGGTG GCGGTCGAAA ACTGCTGGCG CAGCCCGGCG ACGCCGTTCG ACCGCGCTTG CGTCGGGCTG GTCGCGCAGG CGGCGGCCGC ATTCGGCTAC ACGCACGAAC GGATCGTGAG CGGCGCCGGC CACGACGCGA TCCTGCTCGC ACGCCGCTTC CCGACCGCGA TGGTGTTCAT TCCGTGCGTC GACGGCCTGT CGCACAACGA AGCCGAGGAT GCGTTGCCCG ACGACGTGAC GCGCGGCACG AACGTGCTGC TCCATGCGGT GCTGGCGCGG GCCGGCATCG CGGCGCGCGT CGACGCGGCG GCTGCCGCGC ACGGCGCCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT