SSGCID: Target Page

BuceA.11726.a

Putative N-acetylmuramoyl-L-alanine amidase

CENTER ID:	BuceA.11726.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.11726.a.A2.GE33070	Mature protein( BuceA.11726.a )		44	504

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.11726.a.A2.PS01314	Mature protein( BuceA.11726.a )		44	504

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2874
RefSeq:	YP_002231879.1
UniProt:	B4E9H6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLIKPFRSIE SAATATHNWR RRQILCAGAS TLVLGLVAPR LAHASSVLGV RVWPARDYTR VTIESDQPLQ NSQQLLQGPD RLVVDLSGLD LDQALRDLVS KIAPNDPQIQ SVRVGQYQPH VVRMVFDLKG SVKPQVFTLP PVGTYKYRLV FDLYPAVAPD PLSDLIAQTE RKEQALNDTA RAQQMQPPST LAGPGTPPPG AGDNSDAFFQ RFAQNTPTTP HTPPAAGTPS TPAKPAVKPP PVIARRDDGD DDGDTYKFTA PKSGKGGTVR LLTVAIDPGH GGEDPGAIGG NGTYEKHIAL DIAKKLRAKI DAAPNMRAMM TRDADFFVPL NVRVQKARRV GADLFVSIHA DAFTTPSARG SSVFALSDHG ATSAAARWMA NKENSSDLIG GINIKTADAA VNRALFDMST TAQIRDSLRY GNYVLKEVGG INKLHKGSVE QAGFAVLKAP DIPSILVETA FISNPDEERR LNDDSYRDEM ADAIFRGIKR YFAANPPLAK SRMA

NT Sequence

ATGTTGATCA AACCGTTCCG CTCGATCGAA TCGGCGGCCA CCGCGACGCA CAACTGGCGG CGCCGCCAGA TCCTGTGCGC GGGCGCGTCG ACGCTGGTGC TCGGCCTCGT CGCGCCGCGG CTCGCGCACG CGTCGTCGGT GCTCGGCGTG CGCGTGTGGC CCGCACGCGA TTACACGCGC GTCACGATCG AATCCGACCA GCCGCTGCAG AATAGCCAAC AACTGCTGCA GGGTCCCGAC CGTCTCGTCG TCGACCTGAG CGGCCTCGAC CTCGACCAGG CGCTGCGCGA CCTCGTCTCG AAGATCGCGC CGAACGATCC GCAGATCCAG TCGGTGCGCG TCGGCCAGTA TCAGCCGCAC GTCGTGCGGA TGGTGTTCGA CCTGAAAGGA TCGGTGAAGC CGCAGGTGTT CACGCTGCCG CCCGTCGGCA CGTACAAGTA CCGGCTGGTG TTCGACCTGT ATCCGGCCGT CGCGCCCGAT CCGCTGTCCG ACCTGATCGC GCAGACGGAA CGCAAGGAAC AGGCGCTCAA CGACACCGCG CGCGCGCAGC AGATGCAGCC GCCGTCCACG CTGGCGGGCC CCGGCACCCC GCCGCCGGGC GCGGGCGACA ACAGCGACGC ATTCTTCCAG CGTTTCGCGC AGAACACGCC AACTACGCCG CACACGCCGC CGGCGGCCGG CACGCCGTCG ACGCCCGCGA AGCCGGCCGT CAAGCCGCCG CCCGTCATCG CGCGCCGCGA CGACGGCGAC GACGACGGCG ACACCTACAA GTTCACCGCG CCGAAATCCG GCAAGGGCGG CACCGTGCGT CTGCTGACGG TCGCGATCGA CCCGGGCCAC GGCGGCGAGG ATCCGGGCGC GATCGGCGGC AACGGCACGT ACGAGAAGCA CATCGCGCTC GACATCGCGA AGAAGCTGCG CGCGAAGATC GACGCCGCGC CGAACATGCG CGCGATGATG ACGCGCGACG CCGACTTCTT CGTGCCGCTG AACGTGCGCG TGCAGAAGGC GCGCCGCGTC GGCGCGGATC TCTTCGTGTC GATCCACGCG GATGCGTTCA CGACGCCGTC CGCGCGCGGC TCGTCGGTGT TCGCGCTGTC CGACCACGGC GCGACGAGCG CCGCGGCGCG CTGGATGGCG AACAAGGAGA ATTCGTCGGA CCTGATCGGC GGCATCAACA TCAAGACGGC GGACGCGGCG GTGAACCGCG CGCTGTTCGA CATGTCGACG ACCGCGCAGA TCCGCGACTC GCTGCGCTAC GGCAACTACG TGCTGAAGGA AGTGGGCGGC ATCAACAAGC TGCACAAGGG CTCGGTCGAG CAGGCCGGGT TCGCGGTGCT GAAGGCGCCC GACATTCCGT CGATCCTCGT CGAGACCGCA TTCATCAGCA ACCCGGACGA AGAGCGCCGG CTCAACGACG ACAGCTATCG CGACGAGATG GCCGACGCGA TCTTCCGCGG CATCAAGCGT TATTTCGCCG CGAATCCGCC GCTCGCGAAG AGCCGGATGG CC

Details for BuceA.11726.a.A2.GE33070

HARVESTED ON:	8/19/2011
SEQUENCED ON:	8/31/2011
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	71

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMASSVLGVR VWPARDYTRV TIESDQPLQN SQQLLQGPDR LVVDLSGLDL DQALRDLVSK IAPNDPQIQS VRVGQYQPHV VRMVFDLKGS VKPQVFTLPP VGTYKYRLVF DLYPAVAPDP LSDLIAQTER KEQALNDTAR AQQMQPPSTL AGPGTPPPGA GDNSDAFFQR FAQNTPTTPH TPPAAGTPST PAKPAVKPPP VIARRDDXDD DGDTYXFTAP KSGKGGTVRL LTVAIDPGHG GEDPGAIGGN GTYEKHIALD IAKKLRAKID AAPNMRAMMT RDADFFVPLN VRVQKARRVG ADLFVSIHAD AFTTPSARGS SVX

Validated NT Sequence

tanacaccga cgagccgcgc gcggacggcg tcgtgaacgc atccgcgtgg atcgacacga agagatccgc gccgacgcgg cgcgccttct gcacgcgcac gttcagcggc acgaagaagt cggcgtcgcg cgtcatcatc gcgcgcatgt tcggcgcggc gtcgatcttc gcgcgcagct tcttcgcgat gtcgagcgcg atgtgcttct cgtacgtgcc gttgccgccg atcgcgcccg gatcctcgcc gccgtggccc gggtcgatcg cgaccgtcag cagacgcacg gtgccgccct tgccggattt cggcgcggtg aactngtagg tgtcgccgtc gtcgtcgcng tcgtcgcggc gcgcgatgac gggcggcggc ttgacggccg gcttcgcggg cgtcgacggc gtgccggccg ccggcggcgt gtgcggcgta gttggcgtgt tctgcgcgaa acgctggaag aatgcgtcgc tgttgtcgcc cgcgcccggc ggcggggtgc cggggcccgc cagcgtggac ggcggctgca tctgctgcgc gcgcgcggtg tcgttgagcg cctgttcctt gcgttccgtc tgcgcgatca ggtcggacag cggatcgggc gcgacggccg gatacaggtc gaacaccagc cggtacttgt acgtgccgac gggcggcagc gtgaacacct gcggcttcac cgatcctttc aggtcgaaca ccatccgcac gacgtgcggc tgatactggc cgacgcgcac cgactggatc tgcggatcgt tcggcgcgat cttcgagacg aggtcgcgca gcgcctggtc gaggtcgagg ccgctcaggt cgacgacgag acggtcggga ccctgcagca gttgttggct attctgcagc ggctggtcgg attcgatcgt gacgcgcgtg taatcgcgtg cgggccacac gcgcacgccg agcaccgacg acgccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMASSVLGVR VWPARDYTRV TIESDQPLQN SQQLLQGPDR LVVDLSGLDL DQALRDLVSK IAPNDPQIQS VRVGQYQPHV VRMVFDLKGS VKPQVFTLPP VGTYKYRLVF DLYPAVAPDP LSDLIAQTER KEQALNDTAR AQQMQPPSTL AGPGTPPPGA GDNSDAFFQR FAQNTPTTPH TPPAAGTPST PAKPAVKPPP VIARRDDGDD DGDTYKFTAP KSGKGGTVRL LTVAIDPGHG GEDPGAIGGN GTYEKHIALD IAKKLRAKID AAPNMRAMMT RDADFFVPLN VRVQKARRVG ADLFVSIHAD AFTTPSARGS SVFALSDHGA TSAAARWMAN KENSSDLIGG INIKTADAAV NRALFDMSTT AQIRDSLRYG NYVLKEVGGI NKLHKGSVEQ AGFAVLKAPD IPSILVETAF ISNPDEERRL NDDSYRDEMA DAIFRGIKRY FAANPPLAKS RMA

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgtcgtcg gtgctcggcg tgcgcgtgtg gcccgcacgc gattacacgc gcgtcacgat cgaatccgac cagccgctgc agaatagcca acaactgctg cagggtcccg accgtctcgt cgtcgacctg agcggcctcg acctcgacca ggcgctgcgc gacctcgtct cgaagatcgc gccgaacgat ccgcagatcc agtcggtgcg cgtcggccag tatcagccgc acgtcgtgcg gatggtgttc gacctgaaag gatcggtgaa gccgcaggtg ttcacgctgc cgcccgtcgg cacgtacaag taccggctgg tgttcgacct gtatccggcc gtcgcgcccg atccgctgtc cgacctgatc gcgcagacgg aacgcaagga acaggcgctc aacgacaccg cgcgcgcgca gcagatgcag ccgccgtcca cgctggcggg ccccggcacc ccgccgccgg gcgcgggcga caacagcgac gcattcttcc agcgtttcgc gcagaacacg ccaactacgc cgcacacgcc gccggcggcc ggcacgccgt cgacgcccgc gaagccggcc gtcaagccgc cgcccgtcat cgcgcgccgc gacgacggcg acgacgacgg cgacacctac aagttcaccg cgccgaaatc cggcaagggc ggcaccgtgc gtctgctgac ggtcgcgatc gacccgggcc acggcggcga ggatccgggc gcgatcggcg gcaacggcac gtacgagaag cacatcgcgc tcgacatcgc gaagaagctg cgcgcgaaga tcgacgccgc gccgaacatg cgcgcgatga tgacgcgcga cgccgacttc ttcgtgccgc tgaacgtgcg cgtgcagaag gcgcgccgcg tcggcgcgga tctcttcgtg tcgatccacg cggatgcgtt cacgacgccg tccgcgcgcg gctcgtcggt gttcgcgctg tccgaccacg gcgcgacgag cgccgcggcg cgctggatgg cgaacaagga gaattcgtcg gacctgatcg gcggcatcaa catcaagacg gcggacgcgg cggtgaaccg cgcgctgttc gacatgtcga cgaccgcgca gatccgcgac tcgctgcgct acggcaacta cgtgctgaag gaagtgggcg gcatcaacaa gctgcacaag ggctcggtcg agcaggccgg gttcgcggtg ctgaaggcgc ccgacattcc gtcgatcctc gtcgagaccg cattcatcag caacccggac gaagagcgcc ggctcaacga cgacagctat cgcgacgaga tggccgacgc gatcttccgc ggcatcaagc gttatttcgc cgcgaatccg ccgctcgcga agagccggat ggcctaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa

Details for BuceA.11726.a.A2.PS01314

PURIFICATION DATe:	12/20/2011
CONCENTRATION:	42.66mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	3
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	71

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMASSVLGVR VWPARDYTRV TIESDQPLQN SQQLLQGPDR LVVDLSGLDL DQALRDLVSK IAPNDPQIQS VRVGQYQPHV VRMVFDLKGS VKPQVFTLPP VGTYKYRLVF DLYPAVAPDP LSDLIAQTER KEQALNDTAR AQQMQPPSTL AGPGTPPPGA GDNSDAFFQR FAQNTPTTPH TPPAAGTPST PAKPAVKPPP VIARRDDGDD DGDTYKFTAP KSGKGGTVRL LTVAIDPGHG GEDPGAIGGN GTYEKHIALD IAKKLRAKID AAPNMRAMMT RDADFFVPLN VRVQKARRVG ADLFVSIHAD AFTTPSARGS SVFALSDHGA TSAAARWMAN KENSSDLIGG INIKTADAAV NRALFDMSTT AQIRDSLRYG NYVLKEVGGI NKLHKGSVEQ AGFAVLKAPD IPSILVETAF ISNPDEERRL NDDSYRDEMA DAIFRGIKRY FAANPPLAKS RMA

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGTCGTCG GTGCTCGGCG TGCGCGTGTG GCCCGCACGC GATTACACGC GCGTCACGAT CGAATCCGAC CAGCCGCTGC AGAATAGCCA ACAACTGCTG CAGGGTCCCG ACCGTCTCGT CGTCGACCTG AGCGGCCTCG ACCTCGACCA GGCGCTGCGC GACCTCGTCT CGAAGATCGC GCCGAACGAT CCGCAGATCC AGTCGGTGCG CGTCGGCCAG TATCAGCCGC ACGTCGTGCG GATGGTGTTC GACCTGAAAG GATCGGTGAA GCCGCAGGTG TTCACGCTGC CGCCCGTCGG CACGTACAAG TACCGGCTGG TGTTCGACCT GTATCCGGCC GTCGCGCCCG ATCCGCTGTC CGACCTGATC GCGCAGACGG AACGCAAGGA ACAGGCGCTC AACGACACCG CGCGCGCGCA GCAGATGCAG CCGCCGTCCA CGCTGGCGGG CCCCGGCACC CCGCCGCCGG GCGCGGGCGA CAACAGCGAC GCATTCTTCC AGCGTTTCGC GCAGAACACG CCAACTACGC CGCACACGCC GCCGGCGGCC GGCACGCCGT CGACGCCCGC GAAGCCGGCC GTCAAGCCGC CGCCCGTCAT CGCGCGCCGC GACGACGGCG ACGACGACGG CGACACCTAC AAGTTCACCG CGCCGAAATC CGGCAAGGGC GGCACCGTGC GTCTGCTGAC GGTCGCGATC GACCCGGGCC ACGGCGGCGA GGATCCGGGC GCGATCGGCG GCAACGGCAC GTACGAGAAG CACATCGCGC TCGACATCGC GAAGAAGCTG CGCGCGAAGA TCGACGCCGC GCCGAACATG CGCGCGATGA TGACGCGCGA CGCCGACTTC TTCGTGCCGC TGAACGTGCG CGTGCAGAAG GCGCGCCGCG TCGGCGCGGA TCTCTTCGTG TCGATCCACG CGGATGCGTT CACGACGCCG TCCGCGCGCG GCTCGTCGGT GTTCGCGCTG TCCGACCACG GCGCGACGAG CGCCGCGGCG CGCTGGATGG CGAACAAGGA GAATTCGTCG GACCTGATCG GCGGCATCAA CATCAAGACG GCGGACGCGG CGGTGAACCG CGCGCTGTTC GACATGTCGA CGACCGCGCA GATCCGCGAC TCGCTGCGCT ACGGCAACTA CGTGCTGAAG GAAGTGGGCG GCATCAACAA GCTGCACAAG GGCTCGGTCG AGCAGGCCGG GTTCGCGGTG CTGAAGGCGC CCGACATTCC GTCGATCCTC GTCGAGACCG CATTCATCAG CAACCCGGAC GAAGAGCGCC GGCTCAACGA CGACAGCTAT CGCGACGAGA TGGCCGACGC GATCTTCCGC GGCATCAAGC GTTATTTCGC CGCGAATCCG CCGCTCGCGA AGAGCCGGAT GGCCTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA