SSGCID: Target Page

BuceA.11416.a

Chromosome partitioning protein ParA

CENTER ID:	BuceA.11416.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.11416.a.B1.GE37730	Full length( BuceA.11416.a )		1	259

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.30
RefSeq:	YP_002229190.1
UniProt:	B4E583

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MAKIFCVANQ KGGVGKTTTS VNLAASLAAQ EQRVLLIDLD PQGNATMGSG IDKAACEATV YEVLVDGVSV TDARVRPEGV TYDVLPANRE LSGAEIELIS IDNRERRLKA ALERVADDYD FVLIDCPPTL SLLTLNGLCA AHGVVIPMQC EYFALEGLSD LVNTIKQVHA NMNRDLKIIG LLRVMFDPRI TLQQQVSDQL KAHFGDKVFD AVIPRNVRLA EAPSYGLPGV VFDRSSRGAQ AYIQFGAEMI ERVRAFEVS

NT Sequence

ATGGCAAAGA TCTTCTGCGT TGCGAACCAG AAGGGGGGCG TCGGCAAGAC GACGACATCG GTCAATCTCG CCGCAAGCCT TGCAGCGCAG GAGCAACGAG TCCTGTTGAT CGATCTCGAC CCGCAGGGCA ACGCGACGAT GGGCAGCGGG ATCGACAAGG CCGCCTGCGA AGCGACCGTG TATGAAGTGC TGGTCGATGG CGTGTCGGTG ACCGACGCGC GCGTGCGTCC GGAAGGCGTC ACCTACGACG TGCTTCCCGC GAACCGCGAG CTGTCCGGCG CCGAGATCGA GCTGATCAGC ATCGACAATC GCGAGCGCCG GCTGAAGGCC GCGCTCGAGC GCGTGGCCGA CGACTACGAT TTCGTGCTGA TCGATTGCCC GCCGACGCTG TCGCTGCTGA CGCTGAACGG CCTGTGCGCG GCGCACGGCG TCGTGATCCC GATGCAGTGC GAGTACTTCG CGCTGGAAGG GCTGTCGGAC CTCGTCAATA CGATCAAGCA GGTCCACGCG AACATGAATC GCGACCTGAA GATCATCGGC CTGCTGCGCG TGATGTTCGA TCCGCGCATC ACGCTGCAGC AGCAAGTCTC CGATCAACTG AAAGCGCACT TCGGCGACAA GGTGTTCGAC GCGGTGATTC CGCGCAACGT GCGTCTGGCG GAAGCGCCGA GTTACGGGTT GCCGGGCGTC GTGTTCGATC GCAGCTCGCG CGGTGCGCAG GCGTATATCC AGTTCGGTGC CGAGATGATC GAACGCGTGC GCGCGTTCGA GGTGTCG

Details for BuceA.11416.a.B1.GE37730

HARVESTED ON:	2/5/2014
SEQUENCED ON:	2/6/2014
EXPECTED MW:	29kDa
OBSERVED MW:	29kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMA KIFCVANQKG GVGKTTTSVN LAASLAAQEQ RVLLIDLDPQ GNATMGSGID KAACEATVYE VLVDGVSVTD ARVRPEGVTY DVLPANRELS GAEIELISID NRERRLKAAL ERVADDYDFV LIDCPPTLSL LTLNGLCAAH GVVIPMQCEY FALEGLSDLV NTIKQVHANM NRDLKIIGLL RVMFDPRITL QQQVSDQLKA HFGDKVFDAV IPRNVRLAEA PSYGLPGVVF DRSSRGAQAY IQFGAEMIER VRAFEVS

Validated NT Sequence

atcttactca cttacgacac ctcgaacgcg cgcacgcgtt cgatcatctc ggcaccgaac tggatatacg cctgcgcacc gcgcgagctg cgatcgaaca cgacgcccgg caacccgtaa ctcggcgctt ccgccagacg cacgttgcgc ggaatcaccg cgtcgaacac cttgtcgccg aagtgcgctt tcagttgatc ggagacttgc tgctgcagcg tgatgcgcgg atcgaacatc acgcgcagca ggccgatgat cttcaggtcg cgattcatgt tcgcgtggac ctgcttgatc gtattgacga ggtccgacag cccttccagc gcgaagtact cgcactgcat cgggatcacg acgccgtgcg ccgcgcacag gccgttcagc gtcagcagcg acagcgtcgg cgggcaatcg atcagcacga aatcgtagtc gtcggccacg cgctcgagcg cggccttcag ccggcgctcg cgattgtcga tgctgatcag ctcgatctcg gcgccggaca gctcgcggtt cgcgggaagc acgtcgtagg tgacgccttc cggacgcacg cgcgcgtcgg tcaccgacac gccatcgacc agcacttcat acacggtcgc ttcgcaggcg gccttgtcga tcccgctgcc catcgtcgcg ttgccctgcg ggtcgagatc gatcaacagg actcgttgct cctgcgctgc aaggcttgcg gcgagattga ccgatgtcgt cgtcttgccg acgcccccct tctggttcgc aacgcagaag atctttgcca tatggtggtg gtggtggtga gccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggcaa agatcttctg cgttgcgaac cagaaggggg gcgtcggcaa gacgacgaca tcggtcaatc tcgccgcaag ccttgcagcg caggagcaac gagtcctgtt gatcgatctc gacccgcagg gcaacgcgac gatgggcagc gggatcgaca aggccgcctg cgaagcgacc gtgtatgaag tgctggtcga tggcgtgtcg gtgaccgacg cgcgcgtgcg tccggaaggc gtcacctacg acgtgcttcc cgcgaaccgc gagctgtccg gcgccgagat cgagctgatc agcatcgaca atcgcgagcg ccggctgaag gccgcgctcg agcgcgtggc cgacgactac gatttcgtgc tgatcgattg cccgccgacg ctgtcgctgc tgacgctgaa cggcctgtgc gcggcgcacg gcgtcgtgat cccgatgcag tgcgagtact tcgcgctgga agggctgtcg gacctcgtca atacgatcaa gcaggtccac gcgaacatga atcgcgacct gaagatcatc ggcctgctgc gcgtgatgtt cgatccgcgc atcacgctgc agcagcaagt ctccgatcaa ctgaaagcgc acttcggcga caaggtgttc gacgcggtga ttccgcgcaa cgtgcgtctg gcggaagcgc cgagttacgg gttgccgggc gtcgtgttcg atcgcagctc gcgcggtgcg caggcgtata tccagttcgg tgccgagatg atcgaacgcg tgcgcgcgtt cgaggtgtcg tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat