BuceA.10611.a

Putative zinc-binding dehydrogenase

CENTER ID: BuceA.10611.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.10611.a.B1.GE37397 Full length( BuceA.10611.a ) 1 373
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3707
RefSeq: YP_002232668.1
UniProt: B4EG21

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRALQWHGPR DVRLVEIDTP HVGPGDVRIA VEYCGICGSD LHEYADGPHA IPVDVPHPLS RRTAPLTLGH EFCGTVVEVG AGVTTLRAGD RVAVEPEYRC SQCAYCRAGS YNLCVSMGFA GLMGDGGMAD FAVVPAYMLH RLPDGVSLEQ AAVMEPAAVA LHALRRGELR LGETCAVFGL GPIGLLLIML AKRQGATTIV AVDVSPERLA AATRFGATHA FDARGLDASA LQDAIRATTG GLGVDASFEA AGLQATFESA MRALRKGGRV VMVGLMPHAG FDAFRAVNDE LTFTASVGYR HAYEDLLRIV ASGALDLTSI VTRTVSLEDA VADGFDALLA DRTQIKILVS PAQRPARRAT TIGSAEHESS VGV
NT Sequence
ATGCGCGCAC TTCAATGGCA TGGCCCGCGC GACGTCCGTC TCGTGGAAAT CGACACGCCG CACGTCGGCC CCGGCGACGT GCGCATCGCG GTCGAATATT GCGGCATCTG CGGCAGCGAC CTGCACGAAT ATGCGGACGG CCCGCACGCG ATTCCGGTCG ACGTGCCGCA TCCGCTGTCG CGCCGCACCG CGCCGCTGAC GCTCGGCCAC GAGTTCTGCG GCACCGTCGT CGAAGTCGGC GCAGGCGTGA CGACGCTGCG CGCGGGCGAC CGCGTCGCGG TCGAGCCCGA GTATCGCTGC AGCCAGTGCG CGTACTGCCG CGCGGGGTCG TACAACCTGT GCGTGTCGAT GGGCTTCGCC GGGCTGATGG GCGACGGCGG GATGGCCGAC TTCGCGGTCG TGCCCGCCTA CATGCTGCAT CGCCTGCCCG ACGGCGTGAG CCTCGAACAG GCCGCGGTGA TGGAGCCGGC CGCCGTTGCG CTGCACGCGC TGCGACGCGG CGAGCTGCGA CTCGGCGAGA CCTGCGCGGT GTTCGGGCTC GGCCCGATCG GCCTGCTGCT GATCATGCTC GCGAAGCGGC AGGGCGCGAC GACGATCGTC GCCGTCGACG TGTCGCCCGA ACGGCTCGCG GCCGCCACGC GCTTCGGCGC GACGCATGCG TTCGATGCGC GCGGACTCGA TGCGTCCGCG CTGCAGGACG CGATCCGCGC GACCACGGGC GGGCTCGGCG TCGATGCGAG CTTCGAGGCC GCCGGATTGC AGGCCACGTT CGAATCAGCG ATGCGGGCAC TGCGCAAGGG CGGCCGCGTC GTGATGGTGG GCCTGATGCC GCACGCGGGT TTCGACGCGT TCCGCGCGGT CAACGACGAA CTGACCTTCA CCGCGAGCGT CGGCTATCGG CATGCGTACG AAGACCTGCT GCGCATCGTC GCGTCGGGCG CGCTCGACCT CACGTCGATC GTCACGCGCA CCGTGTCGCT CGAAGACGCC GTCGCCGACG GCTTCGACGC GCTGCTCGCC GATCGCACGC AGATCAAGAT CCTCGTTTCA CCCGCGCAGC GGCCAGCCCG CCGCGCCACC ACCATCGGGA GTGCCGAACA TGAATCGTCA GTGGGCGTT
Details for BuceA.10611.a.B1.GE37397
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 25
PERCENT COVERAGE: 94
Validated AA Sequence
MAWPARRPSR GNRHAARRPR RRAHRGRILR HLRQRPARIC GRPARDSGRR AASAVAPHRA ADARPRVLRH RRRSRRRRDD AARGRPRRGR ARVSLQPVRV LPRGVVQPVR VDGLRRADGR RRDGRLRGRA RLHAASPARR REPRTGRGDG AGRRCAARAA TRRAATRRDL RGVRARPDRP AADHAREAAG RDDDRRRRRV ARTARGRHAL RRDACVRCAR TRCVRAAGRD PRDHGRARRR CELRGRRIAG HVRISDAGTA QGRPRRDGGP DAARGFRRVP RGQRRTDLHR ERRLSACVRR PAAHRRVGRA RPHVDRHAHR VARRRRRRRL RRAARRSHAD QDPRFTRAAA SPPRHHHREC
Validated NT Sequence
ggcactcccg atggtggtgg cgcggcgggc tggccgctgc gcgggtgaaa cgaggatctt gatctgcgtg cgatcggcga gcagcgcgtc gaagccgtcg gcgacggcgt cttcgagcga cacggtgcgc gtgacgatcg acgtgaggtc gagcgcgccc gacgcgacga tgcgcagcag gtcttcgtac gcatgccgat agccgacgct cgcggtgaag gtcagttcgt cgttgaccgc gcggaacgcg tcgaaacccg cgtgcggcat caggcccacc atcacgacgc ggccgccctt gcgcagtgcc cgcatcgctg attcgaacgt ggcctgcaat ccggcggcct cgaagctcgc atcgacgccg agcccgcccg tggtcgcgcg gatcgcgtcc tgcagcgcgg acgcatcgag tccgcgcgca tcgaacgcat gcgtcgcgcc gaagcgcgtg gcggccgcga gccgttcggg cgacacgtcg acggcgacga tcgtcgtcgc gccctgccgc ttcgcgagca tgatcagcag caggccgatc gggccgagcc cgaacaccgc gcaggtctcg ccgagtcgca gctcgccgcg tcgcagcgcg tgcagcgcaa cggcggccgg ctccatcacc gcggcctgtt cgaggctcac gccgtcgggc aggcgatgca gcatgtaggc gggcacgacc gcgaagtcgg ccatcccgcc gtcgcccatc agcccggcga agcccatcga cacgcacagg ttgtacgacc ccgcgcggca gtacgcgcac tggctgcagc gatactcggg ctcgaccgcg acgcggtcgc ccgcgcgcag cgtcgtcacg cctgcgccga cttcgacgac ggtgccgcag aactcgtggc cgagcgtcag cggcgcggtg cggcgcgaca gcggatgcgg cacgtcgacc ggaatcgcgt gcgggccgtc cgcatattcg tgcaggtcgc tgccgcagat gccgcaatat tcgaccgcga tgcgcacgtc gccggggccg acgtgcggcg tgtcgatttc cacgagacgg acgtcgcgcg ggccatgcca ttgaagtg
Expected Protein Sequence
MAHHHHHHMR ALQWHGPRDV RLVEIDTPHV GPGDVRIAVE YCGICGSDLH EYADGPHAIP VDVPHPLSRR TAPLTLGHEF CGTVVEVGAG VTTLRAGDRV AVEPEYRCSQ CAYCRAGSYN LCVSMGFAGL MGDGGMADFA VVPAYMLHRL PDGVSLEQAA VMEPAAVALH ALRRGELRLG ETCAVFGLGP IGLLLIMLAK RQGATTIVAV DVSPERLAAA TRFGATHAFD ARGLDASALQ DAIRATTGGL GVDASFEAAG LQATFESAMR ALRKGGRVVM VGLMPHAGFD AFRAVNDELT FTASVGYRHA YEDLLRIVAS GALDLTSIVT RTVSLEDAVA DGFDALLADR TQIKILVSPA QRPARRATTI GSAEHESSVG V
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcgcg cacttcaatg gcatggcccg cgcgacgtcc gtctcgtgga aatcgacacg ccgcacgtcg gccccggcga cgtgcgcatc gcggtcgaat attgcggcat ctgcggcagc gacctgcacg aatatgcgga cggcccgcac gcgattccgg tcgacgtgcc gcatccgctg tcgcgccgca ccgcgccgct gacgctcggc cacgagttct gcggcaccgt cgtcgaagtc ggcgcaggcg tgacgacgct gcgcgcgggc gaccgcgtcg cggtcgagcc cgagtatcgc tgcagccagt gcgcgtactg ccgcgcgggg tcgtacaacc tgtgcgtgtc gatgggcttc gccgggctga tgggcgacgg cgggatggcc gacttcgcgg tcgtgcccgc ctacatgctg catcgcctgc ccgacggcgt gagcctcgaa caggccgcgg tgatggagcc ggccgccgtt gcgctgcacg cgctgcgacg cggcgagctg cgactcggcg agacctgcgc ggtgttcggg ctcggcccga tcggcctgct gctgatcatg ctcgcgaagc ggcagggcgc gacgacgatc gtcgccgtcg acgtgtcgcc cgaacggctc gcggccgcca cgcgcttcgg cgcgacgcat gcgttcgatg cgcgcggact cgatgcgtcc gcgctgcagg acgcgatccg cgcgaccacg ggcgggctcg gcgtcgatgc gagcttcgag gccgccggat tgcaggccac gttcgaatca gcgatgcggg cactgcgcaa gggcggccgc gtcgtgatgg tgggcctgat gccgcacgcg ggtttcgacg cgttccgcgc ggtcaacgac gaactgacct tcaccgcgag cgtcggctat cggcatgcgt acgaagacct gctgcgcatc gtcgcgtcgg gcgcgctcga cctcacgtcg atcgtcacgc gcaccgtgtc gctcgaagac gccgtcgccg acggcttcga cgcgctgctc gccgatcgca cgcagatcaa gatcctcgtt tcacccgcgc agcggccagc ccgccgcgcc accaccatcg ggagtgccga acatgaatcg tcagtgggcg tttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat