BuceA.10478.a
Putative D-amino acid aminotransferase
| CENTER ID: | BuceA.10478.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | False |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.10478.a.B1.GE37396 | Full length( BuceA.10478.a ) | 1 | 308 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.10478.a.B1.PS01958 | Full length( BuceA.10478.a ) | 1 | 308 |
Structures
4PBC
DEPOSITED: 4/12/2014
DETERMINATION: XRay
CLONE: BuceA.10478.a.B1.GE37396
PROTEIN: BuceA.10478.a.B1.PS01958
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| BV-BRC: | fig|216591.5.peg.740 |
| RefSeq: | YP_002229863.1 |
| UniProt: | B4E9Q5 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQAEFEPIV YLSVSAQEEL VPLSEARVPV LDRGFIFGDG VYEVVPVYAH DGAHVPFRIE QHLDRLARSL KKIGIDNPHD AAGWRALIER VVAANAEGLG DGNALVYLQV TRGVAKRGHA FPANAVPTVF AMTSPLRLPS EEERAKGVRC VTAEDRRWLH CDIKSISLLG NVLMAQHAAE RDAFETIQLR DENVTEGSSS NVWIVKNGEL FAPPRSNKIL EGIRYALVEQ LADECGIRFV AREISEVELR SADEIMLTSA TKEILPVTSL DDLPVQGGKP GPVFAALYDA YQRAKAREFE QFDLTRRK
NT Sequence
ATGAGCCAAG CCGAATTCGA ACCGATCGTC TACCTCAGCG TCTCGGCGCA GGAGGAACTG GTGCCGCTGT CCGAAGCCCG TGTGCCGGTG CTCGACCGCG GATTCATCTT CGGCGACGGC GTGTATGAAG TCGTCCCCGT TTATGCGCAC GACGGCGCGC ACGTGCCGTT CCGGATCGAA CAGCATCTGG ACCGGCTCGC GCGCAGCCTG AAGAAGATCG GCATCGACAA CCCGCACGAT GCGGCGGGCT GGCGCGCGTT GATCGAGCGG GTGGTCGCGG CGAACGCCGA AGGCCTCGGC GACGGCAACG CGCTCGTCTA CCTGCAGGTG ACGCGCGGCG TCGCGAAGCG CGGCCACGCG TTCCCGGCGA ACGCGGTGCC GACGGTGTTC GCGATGACGA GCCCGCTGCG CCTGCCGTCG GAAGAGGAGC GCGCGAAGGG CGTGCGCTGC GTGACGGCCG AGGACCGTCG CTGGCTGCAT TGCGACATCA AGTCGATCTC GCTGCTCGGC AACGTGTTGA TGGCGCAGCA TGCGGCCGAG CGCGATGCGT TCGAGACGAT CCAGCTGCGC GACGAAAACG TGACGGAAGG CTCGTCGTCG AACGTGTGGA TCGTGAAGAA CGGCGAACTG TTCGCGCCGC CGCGCAGCAA CAAGATCCTC GAAGGGATCC GCTACGCGCT GGTCGAGCAG CTGGCCGACG AATGCGGGAT TCGGTTCGTC GCGCGCGAGA TCAGCGAGGT CGAACTGCGC TCGGCCGACG AGATCATGCT GACGTCGGCC ACGAAGGAAA TCCTGCCTGT GACGTCGCTC GACGATCTGC CCGTGCAGGG CGGCAAGCCG GGCCCCGTGT TCGCGGCGCT GTATGACGCG TACCAGCGTG CGAAGGCACG CGAGTTTGAA CAGTTTGACC TAACCCGGAG AAAA
Details for BuceA.10478.a.B1.GE37396
| HARVESTED ON: | 9/27/2013 |
| SEQUENCED ON: | 10/3/2013 |
| EXPECTED MW: | 35kDa |
| OBSERVED MW: | 36kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMS QAEFEPIVYL SVSAQEELVP LSEARVPVLD RGFIFGDGVY EVVPVYAHDG AHVPFRIEQH LDRLARSLKK IGIDNPHDAA GWRALIERVV AANAEGLGDG NALVYLQVTR GVAKRGHAFP ANAVPTVFAM TSPLRLPSEE ERAKGVRCVT AEDRRWLHCD IKSISLLGNV LMAQHAAERD AFETIQLRDE NVTEGSSSNV WIVKNGELFA PPRSNKILEG IRYALVEQLA DECGIRFVAR EISEVELRSA DEIMLTSATK EILPVTSLDD LPVQGGKPGP VFAALYDAYQ RAKAREF
Validated NT Sequence
tcaaactcgc gtgccttcgc acgctggtac gcgtcataca gcgccgcgaa cacggggccc ggcttgccgc cctgcacggg cagatcgtcg agcgacgtca caggcaggat ttccttcgtg gccgacgtca gcatgatctc gtcggccgag cgcagttcga cctcgctgat ctcgcgcgcg acgaaccgaa tcccgcattc gtcggccagc tgctcgacca gcgcgtagcg gatcccttcg aggatcttgt tgctgcgcgg cggcgcgaac agttcgccgt tcttcacgat ccacacgttc gacgacgagc cttccgtcac gttttcgtcg cgcagctgga tcgtctcgaa cgcatcgcgc tcggccgcat gctgcgccat caacacgttg ccgagcagcg agatcgactt gatgtcgcaa tgcagccagc gacggtcctc ggccgtcacg cagcgcacgc ccttcgcgcg ctcctcttcc gacggcaggc gcagcgggct cgtcatcgcg aacaccgtcg gcaccgcgtt cgccgggaac gcgtggccgc gcttcgcgac gccgcgcgtc acctgcaggt agacgagcgc gttgccgtcg ccgaggcctt cggcgttcgc cgcgaccacc cgctcgatca acgcgcgcca gcccgccgca tcgtgcgggt tgtcgatgcc gatcttcttc aggctgcgcg cgagccggtc cagatgctgt tcgatccgga acggcacgtg cgcgccgtcg tgcgcataaa cggggacgac ttcatacacg ccgtcgccga agatgaatcc gcggtcgagc accggcacac gggcttcgga cagcggcacc agttcctcct gcgccgagac gctgaggtag acgatcggtt cgaattcggc ttggctcata tggtggtggt ggtggtgagc cat
Expected Protein Sequence
MAHHHHHHMS QAEFEPIVYL SVSAQEELVP LSEARVPVLD RGFIFGDGVY EVVPVYAHDG AHVPFRIEQH LDRLARSLKK IGIDNPHDAA GWRALIERVV AANAEGLGDG NALVYLQVTR GVAKRGHAFP ANAVPTVFAM TSPLRLPSEE ERAKGVRCVT AEDRRWLHCD IKSISLLGNV LMAQHAAERD AFETIQLRDE NVTEGSSSNV WIVKNGELFA PPRSNKILEG IRYALVEQLA DECGIRFVAR EISEVELRSA DEIMLTSATK EILPVTSLDD LPVQGGKPGP VFAALYDAYQ RAKAREFEQF DLTRRK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc aagccgaatt cgaaccgatc gtctacctca gcgtctcggc gcaggaggaa ctggtgccgc tgtccgaagc ccgtgtgccg gtgctcgacc gcggattcat cttcggcgac ggcgtgtatg aagtcgtccc cgtttatgcg cacgacggcg cgcacgtgcc gttccggatc gaacagcatc tggaccggct cgcgcgcagc ctgaagaaga tcggcatcga caacccgcac gatgcggcgg gctggcgcgc gttgatcgag cgggtggtcg cggcgaacgc cgaaggcctc ggcgacggca acgcgctcgt ctacctgcag gtgacgcgcg gcgtcgcgaa gcgcggccac gcgttcccgg cgaacgcggt gccgacggtg ttcgcgatga cgagcccgct gcgcctgccg tcggaagagg agcgcgcgaa gggcgtgcgc tgcgtgacgg ccgaggaccg tcgctggctg cattgcgaca tcaagtcgat ctcgctgctc ggcaacgtgt tgatggcgca gcatgcggcc gagcgcgatg cgttcgagac gatccagctg cgcgacgaaa acgtgacgga aggctcgtcg tcgaacgtgt ggatcgtgaa gaacggcgaa ctgttcgcgc cgccgcgcag caacaagatc ctcgaaggga tccgctacgc gctggtcgag cagctggccg acgaatgcgg gattcggttc gtcgcgcgcg agatcagcga ggtcgaactg cgctcggccg acgagatcat gctgacgtcg gccacgaagg aaatcctgcc tgtgacgtcg ctcgacgatc tgcccgtgca gggcggcaag ccgggccccg tgttcgcggc gctgtatgac gcgtaccagc gtgcgaaggc acgcgagttt gaacagtttg acctaacccg gagaaaatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for BuceA.10478.a.B1.PS01958
| PURIFICATION DATe: | 1/7/2014 |
| CONCENTRATION: | 31mg/ml |
| OBSERVED MW: | 35kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 15 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS QAEFEPIVYL SVSAQEELVP LSEARVPVLD RGFIFGDGVY EVVPVYAHDG AHVPFRIEQH LDRLARSLKK IGIDNPHDAA GWRALIERVV AANAEGLGDG NALVYLQVTR GVAKRGHAFP ANAVPTVFAM TSPLRLPSEE ERAKGVRCVT AEDRRWLHCD IKSISLLGNV LMAQHAAERD AFETIQLRDE NVTEGSSSNV WIVKNGELFA PPRSNKILEG IRYALVEQLA DECGIRFVAR EISEVELRSA DEIMLTSATK EILPVTSLDD LPVQGGKPGP VFAALYDAYQ RAKAREF
Validated NT Sequence
tcaaactcgc gtgccttcgc acgctggtac gcgtcataca gcgccgcgaa cacggggccc ggcttgccgc cctgcacggg cagatcgtcg agcgacgtca caggcaggat ttccttcgtg gccgacgtca gcatgatctc gtcggccgag cgcagttcga cctcgctgat ctcgcgcgcg acgaaccgaa tcccgcattc gtcggccagc tgctcgacca gcgcgtagcg gatcccttcg aggatcttgt tgctgcgcgg cggcgcgaac agttcgccgt tcttcacgat ccacacgttc gacgacgagc cttccgtcac gttttcgtcg cgcagctgga tcgtctcgaa cgcatcgcgc tcggccgcat gctgcgccat caacacgttg ccgagcagcg agatcgactt gatgtcgcaa tgcagccagc gacggtcctc ggccgtcacg cagcgcacgc ccttcgcgcg ctcctcttcc gacggcaggc gcagcgggct cgtcatcgcg aacaccgtcg gcaccgcgtt cgccgggaac gcgtggccgc gcttcgcgac gccgcgcgtc acctgcaggt agacgagcgc gttgccgtcg ccgaggcctt cggcgttcgc cgcgaccacc cgctcgatca acgcgcgcca gcccgccgca tcgtgcgggt tgtcgatgcc gatcttcttc aggctgcgcg cgagccggtc cagatgctgt tcgatccgga acggcacgtg cgcgccgtcg tgcgcataaa cggggacgac ttcatacacg ccgtcgccga agatgaatcc gcggtcgagc accggcacac gggcttcgga cagcggcacc agttcctcct gcgccgagac gctgaggtag acgatcggtt cgaattcggc ttggctcata tggtggtggt ggtggtgagc cat
Expressed Protein Sequence
MAHHHHHHMS QAEFEPIVYL SVSAQEELVP LSEARVPVLD RGFIFGDGVY EVVPVYAHDG AHVPFRIEQH LDRLARSLKK IGIDNPHDAA GWRALIERVV AANAEGLGDG NALVYLQVTR GVAKRGHAFP ANAVPTVFAM TSPLRLPSEE ERAKGVRCVT AEDRRWLHCD IKSISLLGNV LMAQHAAERD AFETIQLRDE NVTEGSSSNV WIVKNGELFA PPRSNKILEG IRYALVEQLA DECGIRFVAR EISEVELRSA DEIMLTSATK EILPVTSLDD LPVQGGKPGP VFAALYDAYQ RAKAREFEQF DLTRRK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCC AAGCCGAATT CGAACCGATC GTCTACCTCA GCGTCTCGGC GCAGGAGGAA CTGGTGCCGC TGTCCGAAGC CCGTGTGCCG GTGCTCGACC GCGGATTCAT CTTCGGCGAC GGCGTGTATG AAGTCGTCCC CGTTTATGCG CACGACGGCG CGCACGTGCC GTTCCGGATC GAACAGCATC TGGACCGGCT CGCGCGCAGC CTGAAGAAGA TCGGCATCGA CAACCCGCAC GATGCGGCGG GCTGGCGCGC GTTGATCGAG CGGGTGGTCG CGGCGAACGC CGAAGGCCTC GGCGACGGCA ACGCGCTCGT CTACCTGCAG GTGACGCGCG GCGTCGCGAA GCGCGGCCAC GCGTTCCCGG CGAACGCGGT GCCGACGGTG TTCGCGATGA CGAGCCCGCT GCGCCTGCCG TCGGAAGAGG AGCGCGCGAA GGGCGTGCGC TGCGTGACGG CCGAGGACCG TCGCTGGCTG CATTGCGACA TCAAGTCGAT CTCGCTGCTC GGCAACGTGT TGATGGCGCA GCATGCGGCC GAGCGCGATG CGTTCGAGAC GATCCAGCTG CGCGACGAAA ACGTGACGGA AGGCTCGTCG TCGAACGTGT GGATCGTGAA GAACGGCGAA CTGTTCGCGC CGCCGCGCAG CAACAAGATC CTCGAAGGGA TCCGCTACGC GCTGGTCGAG CAGCTGGCCG ACGAATGCGG GATTCGGTTC GTCGCGCGCG AGATCAGCGA GGTCGAACTG CGCTCGGCCG ACGAGATCAT GCTGACGTCG GCCACGAAGG AAATCCTGCC TGTGACGTCG CTCGACGATC TGCCCGTGCA GGGCGGCAAG CCGGGCCCCG TGTTCGCGGC GCTGTATGAC GCGTACCAGC GTGCGAAGGC ACGCGAGTTT GAACAGTTTG ACCTAACCCG GAGAAAATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT