SSGCID: Target Page

BuceA.10154.a

Histone deacetylase family protein

CENTER ID:	BuceA.10154.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.10154.a.B1.GE37394	Full length( BuceA.10154.a )		1	375

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.10154.a.B1.PS01982	Full length( BuceA.10154.a )		1	375

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2081
RefSeq:	YP_002231131.1
UniProt:	B4EBN1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTGHASMNRT AFFTDERTFW HTGGTHALFF PVGGWVQPPS SAGYAESPDS KRRFLSLVQA SGLAAQLDLR GAAPATTADL LRIHPAHYLD AFRALSDANG GDLGDLAPFG KGSYEIAALS AGLAIAAVDA VVGERAANAF SLSRPPGHHC LRDRPMGFCM LANIPIAIEA ARAKHGIERV AVIDWDVHHG NGTQSIYYDD PDTLTISLHQ DRCFPPGYSG ADDRGEGAGI GANLNVPLLA GSGDDAYRYA FERIVLPALA RFRPELIVIA SGLDASAVDP LARMQLHTDS YRFMTRAVKE AAQRHCGGRL VIVHEGGYSE AYVPFCGLAI VEELAGVRTE VADPMLDLAI AQQPGERFVA FQRELLDELA ASFGL

NT Sequence

GTGACAGGTC ACGCCTCCAT GAATCGCACT GCCTTTTTCA CCGACGAACG CACTTTCTGG CATACCGGCG GCACGCACGC GCTGTTCTTC CCGGTCGGCG GCTGGGTCCA GCCGCCGTCT AGCGCCGGCT ACGCGGAATC GCCCGATTCG AAGCGCCGCT TCCTGTCGCT CGTACAGGCG TCGGGCCTCG CCGCGCAGCT CGACCTGCGC GGCGCCGCGC CCGCGACGAC GGCCGACCTG CTGCGCATTC ATCCGGCGCA CTATCTCGAC GCGTTCCGCG CCCTCAGCGA CGCGAACGGC GGCGACCTCG GCGATCTCGC GCCATTCGGC AAGGGCAGCT ACGAGATCGC CGCGCTGTCG GCCGGCCTCG CGATCGCGGC GGTCGATGCG GTGGTCGGCG AGCGCGCGGC GAATGCGTTC TCGCTGTCGC GCCCCCCCGG CCATCACTGC CTGCGCGACC GCCCGATGGG CTTTTGCATG CTGGCGAACA TCCCGATCGC GATCGAGGCC GCACGCGCGA AGCACGGCAT CGAGCGTGTC GCGGTGATCG ACTGGGACGT GCATCACGGC AACGGCACGC AGTCGATCTA CTACGACGAT CCGGATACGC TGACGATCTC GCTGCACCAG GACCGCTGTT TCCCGCCCGG CTACAGCGGC GCGGACGATC GCGGCGAAGG CGCGGGCATC GGCGCGAACC TGAACGTGCC GCTGCTCGCC GGCAGCGGCG ACGACGCGTA CCGGTATGCG TTCGAGCGGA TCGTGCTGCC GGCGCTCGCG CGCTTCCGCC CCGAGCTGAT CGTCATCGCG AGCGGCCTCG ACGCGAGCGC GGTCGATCCG CTCGCGCGCA TGCAGCTGCA TACCGACAGC TACCGGTTCA TGACGCGCGC GGTGAAGGAA GCCGCGCAGC GCCATTGCGG CGGGCGGCTC GTGATCGTTC ACGAAGGCGG TTATTCGGAG GCGTACGTGC CGTTCTGCGG GCTCGCGATC GTCGAGGAAC TGGCAGGCGT GCGCACCGAG GTCGCCGATC CGATGCTCGA CCTGGCGATC GCGCAGCAAC CGGGCGAGCG GTTCGTCGCG TTCCAGCGCG AACTGCTCGA CGAACTGGCC GCGTCGTTCG GGTTG

Details for BuceA.10154.a.B1.GE37394

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/3/2013
EXPECTED MW:	41kDa
OBSERVED MW:	41kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMV TGHASMNRTA FFTDERTFWH TGGTHALFFP VGGWVQPPSS AGYAESPDSK RRFLSLVQAS GLAAQLDLRG AAPATTADLL RIHPAHYLDA FRALSDANGG DLGDLAPFGK GSYEIAALSA GLAIAAVDAV VGERAANAFS LSRPPGHHCL RDRPMGFCML ANIPIAIEAA RAKHGIERVA VIDWDVHHGN GTQSIYYDDP DTLTISLHQD RCFPPGYSGA DDRGEGAGIG ANLNVPLLAG SGDDAYRYAF ERIVLPALAR FRPELIVIAS GLDASAVDPL ARMQLHTDSY RFMTRAVKEA AQRHCGGRLV IVHEGGYSEA YVPFCGLAIV EELAGVRTEV ADPMLDLAIA QQPGERFVAF QRELLDEL

Validated NT Sequence

gccagttcgt cgagcagttc gcgctggaac gcgacgaacc gctcgcccgg ttgctgcgcg atcgccaggt cgagcatcgg atcggcgacc tcggtgcgca cgcctgccag ttcctcgacg atcgcgagcc cgcagaacgg cacgtacgcc tccgaataac cgccttcgtg aacgatcacg agccgcccgc cgcaatggcg ctgcgcggct tccttcaccg cgcgcgtcat gaaccggtag ctgtcggtat gcagctgcat gcgcgcgagc ggatcgaccg cgctcgcgtc gaggccgctc gcgatgacga tcagctcggg gcggaagcgc gcgagcgccg gcagcacgat ccgctcgaac gcataccggt acgcgtcgtc gccgctgccg gcgagcagcg gcacgttcag gttcgcgccg atgcccgcgc cttcgccgcg atcgtccgcg ccgctgtagc cgggcgggaa acagcggtcc tggtgcagcg agatcgtcag cgtatccgga tcgtcgtagt agatcgactg cgtgccgttg ccgtgatgca cgtcccagtc gatcaccgcg acacgctcga tgccgtgctt cgcgcgtgcg gcctcgatcg cgatcgggat gttcgccagc atgcaaaagc ccatcgggcg gtcgcgcagg cagtgatggc cgggggggcg cgacagcgag aacgcattcg ccgcgcgctc gccgaccacc gcatcgaccg ccgcgatcgc gaggccggcc gacagcgcgg cgatctcgta gctgcccttg ccgaatggcg cgagatcgcc gaggtcgccg ccgttcgcgt cgctgagggc gcggaacgcg tcgagatagt gcgccggatg aatgcgcagc aggtcggccg tcgtcgcggg cgcggcgccg cgcaggtcga gctgcgcggc gaggcccgac gcctgtacga gcgacaggaa gcggcgcttc gaatcgggcg attccgcgta gccggcgcta gacggcggct ggacccagcc gccgaccggg aagaacagcg cgtgcgtgcc gccggtatgc cagaaagtgc gttcgtcggt gaaaaaggca gtgcgattca tggaggcgtg acctgtcacc atatggtggt ggtggtggtg agccatgg

Expected Protein Sequence

MAHHHHHHMT GHASMNRTAF FTDERTFWHT GGTHALFFPV GGWVQPPSSA GYAESPDSKR RFLSLVQASG LAAQLDLRGA APATTADLLR IHPAHYLDAF RALSDANGGD LGDLAPFGKG SYEIAALSAG LAIAAVDAVV GERAANAFSL SRPPGHHCLR DRPMGFCMLA NIPIAIEAAR AKHGIERVAV IDWDVHHGNG TQSIYYDDPD TLTISLHQDR CFPPGYSGAD DRGEGAGIGA NLNVPLLAGS GDDAYRYAFE RIVLPALARF RPELIVIASG LDASAVDPLA RMQLHTDSYR FMTRAVKEAA QRHCGGRLVI VHEGGYSEAY VPFCGLAIVE ELAGVRTEVA DPMLDLAIAQ QPGERFVAFQ RELLDELAAS FGL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgtgacag gtcacgcctc catgaatcgc actgcctttt tcaccgacga acgcactttc tggcataccg gcggcacgca cgcgctgttc ttcccggtcg gcggctgggt ccagccgccg tctagcgccg gctacgcgga atcgcccgat tcgaagcgcc gcttcctgtc gctcgtacag gcgtcgggcc tcgccgcgca gctcgacctg cgcggcgccg cgcccgcgac gacggccgac ctgctgcgca ttcatccggc gcactatctc gacgcgttcc gcgccctcag cgacgcgaac ggcggcgacc tcggcgatct cgcgccattc ggcaagggca gctacgagat cgccgcgctg tcggccggcc tcgcgatcgc ggcggtcgat gcggtggtcg gcgagcgcgc ggcgaatgcg ttctcgctgt cgcgcccccc cggccatcac tgcctgcgcg accgcccgat gggcttttgc atgctggcga acatcccgat cgcgatcgag gccgcacgcg cgaagcacgg catcgagcgt gtcgcggtga tcgactggga cgtgcatcac ggcaacggca cgcagtcgat ctactacgac gatccggata cgctgacgat ctcgctgcac caggaccgct gtttcccgcc cggctacagc ggcgcggacg atcgcggcga aggcgcgggc atcggcgcga acctgaacgt gccgctgctc gccggcagcg gcgacgacgc gtaccggtat gcgttcgagc ggatcgtgct gccggcgctc gcgcgcttcc gccccgagct gatcgtcatc gcgagcggcc tcgacgcgag cgcggtcgat ccgctcgcgc gcatgcagct gcataccgac agctaccggt tcatgacgcg cgcggtgaag gaagccgcgc agcgccattg cggcgggcgg ctcgtgatcg ttcacgaagg cggttattcg gaggcgtacg tgccgttctg cgggctcgcg atcgtcgagg aactggcagg cgtgcgcacc gaggtcgccg atccgatgct cgacctggcg atcgcgcagc aaccgggcga gcggttcgtc gcgttccagc gcgaactgct cgacgaactg gccgcgtcgt tcgggttgtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for BuceA.10154.a.B1.PS01982

PURIFICATION DATe:	1/30/2014
CONCENTRATION:	51.3mg/ml
OBSERVED MW:	38kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	6
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGTGACAG GTCACGCCTC CATGAATCGC ACTGCCTTTT TCACCGACGA ACGCACTTTC TGGCATACCG GCGGCACGCA CGCGCTGTTC TTCCCGGTCG GCGGCTGGGT CCAGCCGCCG TCTAGCGCCG GCTACGCGGA ATCGCCCGAT TCGAAGCGCC GCTTCCTGTC GCTCGTACAG GCGTCGGGCC TCGCCGCGCA GCTCGACCTG CGCGGCGCCG CGCCCGCGAC GACGGCCGAC CTGCTGCGCA TTCATCCGGC GCACTATCTC GACGCGTTCC GCGCCCTCAG CGACGCGAAC GGCGGCGACC TCGGCGATCT CGCGCCATTC GGCAAGGGCA GCTACGAGAT CGCCGCGCTG TCGGCCGGCC TCGCGATCGC GGCGGTCGAT GCGGTGGTCG GCGAGCGCGC GGCGAATGCG TTCTCGCTGT CGCGCCCCCC CGGCCATCAC TGCCTGCGCG ACCGCCCGAT GGGCTTTTGC ATGCTGGCGA ACATCCCGAT CGCGATCGAG GCCGCACGCG CGAAGCACGG CATCGAGCGT GTCGCGGTGA TCGACTGGGA CGTGCATCAC GGCAACGGCA CGCAGTCGAT CTACTACGAC GATCCGGATA CGCTGACGAT CTCGCTGCAC CAGGACCGCT GTTTCCCGCC CGGCTACAGC GGCGCGGACG ATCGCGGCGA AGGCGCGGGC ATCGGCGCGA ACCTGAACGT GCCGCTGCTC GCCGGCAGCG GCGACGACGC GTACCGGTAT GCGTTCGAGC GGATCGTGCT GCCGGCGCTC GCGCGCTTCC GCCCCGAGCT GATCGTCATC GCGAGCGGCC TCGACGCGAG CGCGGTCGAT CCGCTCGCGC GCATGCAGCT GCATACCGAC AGCTACCGGT TCATGACGCG CGCGGTGAAG GAAGCCGCGC AGCGCCATTG CGGCGGGCGG CTCGTGATCG TTCACGAAGG CGGTTATTCG GAGGCGTACG TGCCGTTCTG CGGGCTCGCG ATCGTCGAGG AACTGGCAGG CGTGCGCACC GAGGTCGCCG ATCCGATGCT CGACCTGGCG ATCGCGCAGC AACCGGGCGA GCGGTTCGTC GCGTTCCAGC GCGAACTGCT CGACGAACTG GCCGCGTCGT TCGGGTTGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT