SSGCID: Target Page

BuceA.10100.b

Serine peptidase, subfamily S1B

CENTER ID:	BuceA.10100.b
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.10100.b.B2.GE37393	Mature protein( BuceA.10100.b )		31	526

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.10100.b.B2.PS01990	Mature protein( BuceA.10100.b )		31	526	unavailable

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.5471
RefSeq:	YP_002234307.1
UniProt:	B4EKU5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MVCQTLARAV LRTALIGGVL AGILPPPAAI AGTVAPPAAP PAMKARPPAA PYAMPVDFPA IVERYGPAVV TIMAATPDPQ TGGPAFAMLD PDDPLAAFFR HGAPPSAPGP QAPAPDTAPR AVSGSGSGFI VSADGLILTS AHVVDEATDV TVRLTDRREF KATVLAVDPQ SDVAVLRVDA TKLPFVRVGD SSKVRAGEPV MTIGAPDGSG NTVTAGIVSA TSRRLPDGSA FPFFETDIAP NPDNSGGPVF NRAGDVIGIA VQVYTGADRY ASTTFAIPIA FATKVRAQLR AQQQAQVQAQ QAPPPAAEAS SGNAFGVDVQ DVGVGLAAAF GLPRAAGALV NGVAPGSPAA AAGLKPGDVI VKVGDKTIGH SAELNDLAGA LPPGGKARLR VIRNRAPMML TITGADDAGS AVATVGMAAP KAAARPGPGS GAGDAGDHGD RLGLTMHALS DDERRSTGLA VGMMVDAVHG PAARAGIQPG DVVLELNDTL LETPDDVPTL EASGGSVIAV LIQRNNARKF VSVRAR

NT Sequence

GTGGTCTGCC AGACGCTTGC TCGCGCCGTG CTTCGCACCG CGTTGATCGG GGGCGTGCTT GCCGGAATCT TGCCGCCGCC CGCCGCCATC GCCGGCACCG TCGCACCGCC CGCCGCACCG CCCGCGATGA AGGCGCGGCC GCCCGCCGCG CCTTACGCGA TGCCGGTCGA CTTCCCGGCG ATCGTCGAGC GCTACGGCCC GGCGGTCGTG ACCATCATGG CCGCCACGCC CGACCCGCAA ACCGGCGGCC CGGCCTTTGC CATGCTCGAT CCCGACGATC CGCTCGCCGC GTTCTTCCGC CATGGCGCAC CGCCGTCCGC GCCGGGGCCG CAGGCACCGG CGCCCGATAC CGCGCCGCGT GCGGTGTCCG GCAGCGGCTC GGGTTTCATC GTCAGCGCCG ATGGCCTGAT CCTCACGTCC GCTCATGTGG TCGACGAGGC GACCGACGTG ACGGTGCGGC TCACCGACCG GCGCGAATTC AAGGCGACGG TACTGGCCGT CGATCCGCAA AGCGACGTGG CCGTGCTGCG CGTCGACGCG ACGAAGCTGC CGTTCGTGCG CGTCGGCGAT TCGTCGAAAG TGCGCGCGGG CGAACCGGTG ATGACGATCG GCGCGCCGGA CGGCTCGGGC AACACGGTCA CGGCCGGCAT CGTCAGCGCG ACGTCGCGCC GGCTGCCGGA CGGCAGCGCG TTTCCGTTCT TCGAAACCGA CATCGCACCG AACCCCGACA ACTCGGGCGG CCCGGTGTTC AATCGCGCGG GTGACGTGAT CGGCATCGCG GTGCAGGTCT ATACGGGCGC CGACCGCTAC GCGAGCACGA CATTCGCCAT CCCGATCGCG TTCGCGACGA AGGTGCGCGC CCAGTTGCGT GCGCAACAGC AGGCGCAAGT GCAGGCGCAG CAGGCTCCGC CGCCCGCGGC GGAGGCGTCG TCGGGCAATG CGTTCGGCGT CGACGTGCAG GACGTGGGCG TCGGCCTGGC CGCGGCGTTC GGACTGCCGC GTGCGGCGGG CGCACTCGTC AACGGGGTCG CGCCCGGCTC GCCGGCCGCC GCGGCCGGCC TGAAACCCGG CGACGTGATC GTGAAGGTGG GCGACAAGAC GATCGGCCAC TCGGCCGAAC TGAACGATCT GGCCGGCGCG CTGCCACCGG GCGGCAAGGC GCGGCTGCGC GTGATCCGCA ACCGTGCGCC GATGATGCTG ACGATCACCG GCGCCGACGA CGCGGGTAGC GCGGTCGCCA CGGTCGGCAT GGCCGCGCCG AAGGCCGCCG CGCGGCCGGG GCCGGGTTCG GGGGCGGGCG ATGCAGGCGA CCACGGCGAC CGCCTCGGCC TGACGATGCA CGCGTTGAGC GACGACGAGC GCCGTTCGAC CGGGCTCGCG GTCGGGATGA TGGTGGATGC GGTGCATGGC CCGGCCGCGC GCGCCGGTAT CCAGCCGGGC GACGTGGTGC TGGAGCTCAA CGACACGCTG CTCGAGACGC CGGACGACGT ACCGACGCTT GAAGCGAGCG GCGGCAGCGT GATCGCCGTG CTGATCCAGC GCAACAACGC GCGGAAGTTC GTGTCGGTGC GCGCGCGT

Details for BuceA.10100.b.B2.GE37393

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/3/2013
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Validated AA Sequence

MKARPPAAPY AMPVDFPAIV ERYGPAVVTI MAATPDPQTG GPAFAMLDPD DPLAAFFRHG APPSAPGPQA PAPDTAPRAV SGSGSGFIVS ADGLILTSAH VVDEATDVTV RLTDRREFKA TVLAVDPQSD VAVLRVDATK LPFVRVGDSS KVRAGEPVMT IGAPDGSGNT VTAGIVSATS RRLPDGSAFP FFETDIAPNP DNSGGPVFNR AGDVIGIAVQ VYTGADRYAS TTFAIPIAFA TKVRAQLRAQ QQAQVQAQQA PPPAAEASSG NAFGVDVQDV GVGLAAAFGL PRAAGALVNG VAPGSPAAAA GLKPGDVIVK VGDKTIGHSA ELNDLAGALP PGGKARLRVI RNRAPMMLTI TGADDAGSAV ATVGMAAPKA AARPGPGSGA GDAGDHGDRL GLTMHALSDD ERRSTGLAVG MMVDAVHGPA ARAGIQPGDV VLELNDTLLE TPDDVPTLEA SGGSVIAVLI QRNNA

Validated NT Sequence

cgcgcgttgt tgcgctggat cagcacggcg atcacgctgc cgccgctcgc ttcaagcgtc ggtacgtcgt ccggcgtctc gagcagcgtg tcgttgagct ccagcaccac gtcgcccggc tggataccgg cgcgcgcggc cgggccatgc accgcatcca ccatcatccc gaccgcgagc ccggtcgaac ggcgctcgtc gtcgctcaac gcgtgcatcg tcaggccgag gcggtcgccg tggtcgcctg catcgcccgc ccccgaaccc ggccccggcc gcgcggcggc cttcggcgcg gccatgccga ccgtggcgac cgcgctaccc gcgtcgtcgg cgccggtgat cgtcagcatc atcggcgcac ggttgcggat cacgcgcagc cgcgccttgc cgcccggtgg cagcgcgccg gccagatcgt tcagttcggc cgagtggccg atcgtcttgt cgcccacctt cacgatcacg tcgccgggtt tcaggccggc cgcggcggcc ggcgagccgg gcgcgacccc gttgacgagt gcgcccgccg cacgcggcag tccgaacgcc gcggccaggc cgacgcccac gtcctgcacg tcgacgccga acgcattgcc cgacgacgcc tccgccgcgg gcggcggagc ctgctgcgcc tgcacttgcg cctgctgttg cgcacgcaac tgggcgcgca ccttcgtcgc gaacgcgatc gggatggcga atgtcgtgct cgcgtagcgg tcggcgcccg tatagacctg caccgcgatg ccgatcacgt cacccgcgcg attgaacacc gggccgcccg agttgtcggg gttcggtgcg atgtcggttt cgaagaacgg aaacgcgctg ccgtccggca gccggcgcga cgtcgcgctg acgatgccgg ccgtgaccgt gttgcccgag ccgtccggcg cgccgatcgt catcaccggt tcgcccgcgc gcactttcga cgaatcgccg acgcgcacga acggcagctt cgtcgcgtcg acgcgcagca cggccacgtc gctttgcgga tcgacggcca gtaccgtcgc cttgaattcg cgccggtcgg tgagccgcac cgtcacgtcg gtcgcctcgt cgaccacatg agcggacgtg aggatcaggc catcggcgct gacgatgaaa cccgagccgc tgccggacac cgcacgcggc gcggtatcgg gcgccggtgc ctgcggcccc ggcgcggacg gcggtgcgcc atggcggaag aacgcggcga gcggatcgtc gggatcgagc atggcaaagg ccgggccgcc ggtttgcggg tcgggcgtgg cggccatgat ggtcacgacc gccgggccgt agcgctcgac gatcgccggg aagtcgaccg gcatcgcgta aggcgcggcg ggcggccgcg ccttcatcgc gggcggtgcg gcgggcggtg cgacggtgcc ggcc

Expected Protein Sequence

MAHHHHHHAG TVAPPAAPPA MKARPPAAPY AMPVDFPAIV ERYGPAVVTI MAATPDPQTG GPAFAMLDPD DPLAAFFRHG APPSAPGPQA PAPDTAPRAV SGSGSGFIVS ADGLILTSAH VVDEATDVTV RLTDRREFKA TVLAVDPQSD VAVLRVDATK LPFVRVGDSS KVRAGEPVMT IGAPDGSGNT VTAGIVSATS RRLPDGSAFP FFETDIAPNP DNSGGPVFNR AGDVIGIAVQ VYTGADRYAS TTFAIPIAFA TKVRAQLRAQ QQAQVQAQQA PPPAAEASSG NAFGVDVQDV GVGLAAAFGL PRAAGALVNG VAPGSPAAAA GLKPGDVIVK VGDKTIGHSA ELNDLAGALP PGGKARLRVI RNRAPMMLTI TGADDAGSAV ATVGMAAPKA AARPGPGSGA GDAGDHGDRL GLTMHALSDD ERRSTGLAVG MMVDAVHGPA ARAGIQPGDV VLELNDTLLE TPDDVPTLEA SGGSVIAVLI QRNNARKFVS VRAR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgccggca ccgtcgcacc gcccgccgca ccgcccgcga tgaaggcgcg gccgcccgcc gcgccttacg cgatgccggt cgacttcccg gcgatcgtcg agcgctacgg cccggcggtc gtgaccatca tggccgccac gcccgacccg caaaccggcg gcccggcctt tgccatgctc gatcccgacg atccgctcgc cgcgttcttc cgccatggcg caccgccgtc cgcgccgggg ccgcaggcac cggcgcccga taccgcgccg cgtgcggtgt ccggcagcgg ctcgggtttc atcgtcagcg ccgatggcct gatcctcacg tccgctcatg tggtcgacga ggcgaccgac gtgacggtgc ggctcaccga ccggcgcgaa ttcaaggcga cggtactggc cgtcgatccg caaagcgacg tggccgtgct gcgcgtcgac gcgacgaagc tgccgttcgt gcgcgtcggc gattcgtcga aagtgcgcgc gggcgaaccg gtgatgacga tcggcgcgcc ggacggctcg ggcaacacgg tcacggccgg catcgtcagc gcgacgtcgc gccggctgcc ggacggcagc gcgtttccgt tcttcgaaac cgacatcgca ccgaaccccg acaactcggg cggcccggtg ttcaatcgcg cgggtgacgt gatcggcatc gcggtgcagg tctatacggg cgccgaccgc tacgcgagca cgacattcgc catcccgatc gcgttcgcga cgaaggtgcg cgcccagttg cgtgcgcaac agcaggcgca agtgcaggcg cagcaggctc cgccgcccgc ggcggaggcg tcgtcgggca atgcgttcgg cgtcgacgtg caggacgtgg gcgtcggcct ggccgcggcg ttcggactgc cgcgtgcggc gggcgcactc gtcaacgggg tcgcgcccgg ctcgccggcc gccgcggccg gcctgaaacc cggcgacgtg atcgtgaagg tgggcgacaa gacgatcggc cactcggccg aactgaacga tctggccggc gcgctgccac cgggcggcaa ggcgcggctg cgcgtgatcc gcaaccgtgc gccgatgatg ctgacgatca ccggcgccga cgacgcgggt agcgcggtcg ccacggtcgg catggccgcg ccgaaggccg ccgcgcggcc ggggccgggt tcgggggcgg gcgatgcagg cgaccacggc gaccgcctcg gcctgacgat gcacgcgttg agcgacgacg agcgccgttc gaccgggctc gcggtcggga tgatggtgga tgcggtgcat ggcccggccg cgcgcgccgg tatccagccg ggcgacgtgg tgctggagct caacgacacg ctgctcgaga cgccggacga cgtaccgacg cttgaagcga gcggcggcag cgtgatcgcc gtgctgatcc agcgcaacaa cgcgcggaag ttcgtgtcgg tgcgcgcgcg ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BuceA.10100.b.B2.PS01990

PURIFICATION DATe:	2/5/2014
CONCENTRATION:	11.72mg/ml
OBSERVED MW:	60kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	data unavailable
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	94

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGCCGGCA CCGTCGCACC GCCCGCCGCA CCGCCCGCGA TGAAGGCGCG GCCGCCCGCC GCGCCTTACG CGATGCCGGT CGACTTCCCG GCGATCGTCG AGCGCTACGG CCCGGCGGTC GTGACCATCA TGGCCGCCAC GCCCGACCCG CAAACCGGCG GCCCGGCCTT TGCCATGCTC GATCCCGACG ATCCGCTCGC CGCGTTCTTC CGCCATGGCG CACCGCCGTC CGCGCCGGGG CCGCAGGCAC CGGCGCCCGA TACCGCGCCG CGTGCGGTGT CCGGCAGCGG CTCGGGTTTC ATCGTCAGCG CCGATGGCCT GATCCTCACG TCCGCTCATG TGGTCGACGA GGCGACCGAC GTGACGGTGC GGCTCACCGA CCGGCGCGAA TTCAAGGCGA CGGTACTGGC CGTCGATCCG CAAAGCGACG TGGCCGTGCT GCGCGTCGAC GCGACGAAGC TGCCGTTCGT GCGCGTCGGC GATTCGTCGA AAGTGCGCGC GGGCGAACCG GTGATGACGA TCGGCGCGCC GGACGGCTCG GGCAACACGG TCACGGCCGG CATCGTCAGC GCGACGTCGC GCCGGCTGCC GGACGGCAGC GCGTTTCCGT TCTTCGAAAC CGACATCGCA CCGAACCCCG ACAACTCGGG CGGCCCGGTG TTCAATCGCG CGGGTGACGT GATCGGCATC GCGGTGCAGG TCTATACGGG CGCCGACCGC TACGCGAGCA CGACATTCGC CATCCCGATC GCGTTCGCGA CGAAGGTGCG CGCCCAGTTG CGTGCGCAAC AGCAGGCGCA AGTGCAGGCG CAGCAGGCTC CGCCGCCCGC GGCGGAGGCG TCGTCGGGCA ATGCGTTCGG CGTCGACGTG CAGGACGTGG GCGTCGGCCT GGCCGCGGCG TTCGGACTGC CGCGTGCGGC GGGCGCACTC GTCAACGGGG TCGCGCCCGG CTCGCCGGCC GCCGCGGCCG GCCTGAAACC CGGCGACGTG ATCGTGAAGG TGGGCGACAA GACGATCGGC CACTCGGCCG AACTGAACGA TCTGGCCGGC GCGCTGCCAC CGGGCGGCAA GGCGCGGCTG CGCGTGATCC GCAACCGTGC GCCGATGATG CTGACGATCA CCGGCGCCGA CGACGCGGGT AGCGCGGTCG CCACGGTCGG CATGGCCGCG CCGAAGGCCG CCGCGCGGCC GGGGCCGGGT TCGGGGGCGG GCGATGCAGG CGACCACGGC GACCGCCTCG GCCTGACGAT GCACGCGTTG AGCGACGACG AGCGCCGTTC GACCGGGCTC GCGGTCGGGA TGATGGTGGA TGCGGTGCAT GGCCCGGCCG CGCGCGCCGG TATCCAGCCG GGCGACGTGG TGCTGGAGCT CAACGACACG CTGCTCGAGA CGCCGGACGA CGTACCGACG CTTGAAGCGA GCGGCGGCAG CGTGATCGCC GTGCTGATCC AGCGCAACAA CGCGCGGAAG TTCGTGTCGG TGCGCGCGCG TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT