SSGCID: Target Page

BuceA.01673.v

Multidrug efflux system outer membrane protein

CENTER ID:	BuceA.01673.v
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01673.v.A2.GE31852	Mature protein( BuceA.01673.v )		24	500
BuceA.01673.v.AE2.GE43218	Mature protein( BuceA.01673.v )		24	500

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01673.v.A2.PW31765	Mature protein( BuceA.01673.v )		24	500

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.7470
RefSeq:	YP_002154135.1
UniProt:	B4EPF2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MQKHALTAAA VALAAALFAT GCTMAPHYKR PDAPVAQAYP AGGVYATQPG AAGARSANGQ AATAIGWREF FVDPRLQRLI EIALKNNRDL RVSVLNIEAA RAQYQITRAG LFPTLDGTGT GNRQRLPNSL TAVPGRNITT TYNVGLSASW ELDLFGRVQS LKDQALAQYL STSYARQASE ISLVSQVADQ YLTLLSTDDL LKVTEDTLKT AQASYDLTKL QFDNGTGSEL ELRQAQTVVE TALANQQAQA RARAQALNAL VLLIGEPLPD DLPAGMPLDA QNLLTDVPAG LPSDLLTRRP DVMQAEQTLL AANANIGAAR AAFFPKISLT GAFGTASPTL GGLFKAGTAA WSFAPSIALP IFEGGQNIAN LDLANVQKRI EIANYEKAIQ SAFREVSDGL AARGTYDQQI AALERNEHAQ QRRFDLSDLR YKNGVDSYLS VLTAQTDLYA AQQSLISARL ARWTNLVDLY RALGGGWIQR AGETPRAPDA PVDYQTATNG

NT Sequence

ATGCAAAAAC ATGCTTTGAC TGCAGCCGCG GTCGCACTGG CTGCCGCGCT GTTCGCCACG GGCTGCACGA TGGCGCCGCA CTACAAGCGG CCCGACGCAC CCGTCGCGCA GGCGTACCCG GCCGGCGGCG TCTACGCGAC GCAGCCGGGC GCGGCCGGCG CGCGCAGCGC GAATGGCCAG GCGGCGACTG CCATCGGCTG GCGCGAGTTC TTCGTCGATC CGCGCCTGCA GCGGCTGATC GAGATCGCGC TGAAAAACAA CCGCGACCTG CGCGTGTCGG TGCTCAATAT CGAGGCCGCG CGTGCGCAGT ACCAGATCAC GCGTGCGGGG CTGTTCCCGA CGCTCGACGG TACGGGTACG GGCAACCGTC AGCGTTTGCC GAATTCGCTG ACGGCCGTGC CGGGCCGGAA CATCACGACG ACCTACAACG TCGGGCTGTC CGCGTCATGG GAACTCGACC TGTTCGGCCG CGTGCAGAGC CTGAAGGACC AGGCGCTCGC GCAATACCTG TCGACGTCGT ACGCGCGACA GGCGTCCGAG ATCTCGCTGG TGTCGCAGGT CGCGGATCAA TATCTGACGC TGCTGTCGAC CGATGACCTG CTGAAGGTCA CGGAGGACAC GCTGAAGACC GCGCAGGCAT CGTACGACCT GACCAAGCTG CAGTTCGACA ACGGCACGGG CTCCGAGCTC GAGCTGCGTC AGGCGCAGAC GGTGGTCGAG ACGGCGCTCG CGAACCAGCA GGCGCAGGCG CGAGCCCGCG CGCAGGCGCT GAACGCGCTG GTGCTGCTGA TCGGCGAGCC GCTGCCGGAC GATCTGCCGG CGGGCATGCC GCTCGATGCG CAGAACCTGC TGACGGACGT GCCGGCCGGG TTGCCGTCGG ATCTGCTGAC GCGTCGTCCT GACGTGATGC AGGCCGAGCA GACGCTGCTG GCCGCGAACG CCAATATCGG CGCGGCGCGC GCGGCGTTCT TCCCGAAGAT TTCGCTGACG GGCGCGTTCG GCACCGCGAG CCCGACGCTG GGCGGCCTGT TCAAGGCCGG CACGGCGGCG TGGTCGTTCG CGCCGAGCAT CGCGCTGCCG ATCTTCGAAG GCGGGCAGAA TATCGCGAAC CTCGATCTCG CGAACGTGCA GAAGCGCATC GAGATCGCGA ACTACGAGAA GGCGATCCAG TCGGCGTTCC GCGAAGTGTC GGATGGTCTG GCCGCACGCG GCACGTACGA CCAGCAGATC GCGGCGCTCG AGCGCAACGA GCACGCGCAG CAGCGACGCT TCGATCTGTC GGACCTGCGC TACAAGAACG GTGTCGACAG CTACCTGTCG GTGCTGACCG CGCAGACGGA TCTGTACGCC GCGCAGCAGT CGCTGATCAG CGCACGTCTG GCACGCTGGA CGAACCTGGT CGACCTGTAC CGCGCACTGG GCGGCGGCTG GATTCAGCGC GCCGGCGAGA CGCCGCGCGC GCCGGATGCG CCGGTCGATT ACCAGACAGC GACGAACGGA

Details for BuceA.01673.v.A2.GE31852

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	53kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	98
PERCENT COVERAGE:	87

Validated AA Sequence

GPGSMAPHYK RPDAPVAQAY PAGGVYATQP GAAGARSANX QAATAIGWRE FFXDPRLQRL IEIALKNNRD LRVSVLNIEA ARAQYQITRA GLFPTLDGTG TGNRQRLPNS LTAVPGRNIT TTYNVGLSAX XELDLFGRVQ SLKDQALAQY LSTSYXRQAS EISLVSXVAD QYLTLLSTDD LLKVTEDTLK TAQASYDLTK LQFDNGTGSE LELXQAQTVV ETALANQQAQ ARARAQALNA LVLLIGEPLP DDLPAGMPLD AQNLLTDVPA GLPSDLLTRR PDVMQAEQTL LAANANIGAA RAAFFPKISL TGAFGTASPT LGGLFKAGTA AWSFAPSIAL PIFEGGQNIA NLDLANVQKR IEIANYEKAI QSAFREVSDG LAARGTYDQQ IAALERNEHA QQRRFDLSDL RYKNGVDSYL SVXNTXXXRE TTXXSL

Validated NT Sequence

ggtcctggtt cgatggcgcc gcactacaag cggcccgacg cacccgtcgc gcaggcgtac ccggccggcg gcgtctacgc gacgcagccg ggcgcggccg gcgcgcgcag cgcgaatggn caggcggcga ctgccatcgg ctggcgcgag ttcttcntcg atccgcgcct gcagcggctg atcgagatcg cgctgaaaaa caaccgcgac ctgcgcgtgt cggtgctcaa tatcgaggcc gcgcgtgcgc agtaccagat cacgcgtgcg gggctgttcc cgacgctcga cggtacgggt acgggcaacc gtcagcgttt gccgaattcg ctgacggccg tgccgggccg gaacatcacg acgacctaca acgtcgggct gtccgcgnnn ngggaactcg acctgttcgg ccgcgtgcag agcctgaagg accaggcgct cgcgcaatac ctgtcgacgt cgtacncgcg acaggcgtcc gagatctcgc tggtgtcgca ngtcgcggat caatatctga cgctgctgtc gaccgatgac ctgctgaagg tcacggagga cacgctgaag accgcgcagg catcgtacga cctgaccaag ctgcagttcg acaacggcac gggctccgag ctcgagctgc ntcaggcgca gacggtggtc gagacggcgc tcgcgaacca gcaggcgcag gcgcgagccc gcgcgcaggc gctgaacgcg ctggtgctgc tgatcggcga gccgctgccg gacgatctgc cggcgggcat gccgctcgat gcgcagaacc tgctgacgga cgtgccggcc gggttgccgt cggatctgct gacgcgtcgt cctgacgtga tgcaggccga gcagacgctg ctggccgcga acgccaatat cggcgcggcg cgcgcggcgt tcttcccgaa gatttcgctg acgggcgcgt tcggcaccgc gagcccgacg ctgggcggcc tgttcaaggc cggcacggcg gcgtggtcgt tcgcgccgag catcgcgctg ccgatcttcg aaggcgggca gaatatcgcg aacctcgatc tcgcgaacgt gcagaagcgc atcgagatcg cgaactacga gaaggcgatc cagtcggcgt tccgcgaagt gtcggatggt ctggccgcac gcggcacgta cgaccagcag atcgcggcgc tcgagcgcaa cgagcacgcg cagcagcgac gcttcgatct gtcggacctg cgctacaaga acggtgtcga cagctacctg tcggtgnnta atacgannnn nnatagggag accacannnn tntccctcta gaaataattt tgnnnnnctt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc cnggaagctc agacccaggg tcnngnttcg atgtctgccc cgaanntcgg gattcgggtc gcataataaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaancccgaa

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMAPHYKRPD APVAQAYPAG GVYATQPGAA GARSANGQAA TAIGWREFFV DPRLQRLIEI ALKNNRDLRV SVLNIEAARA QYQITRAGLF PTLDGTGTGN RQRLPNSLTA VPGRNITTTY NVGLSASWEL DLFGRVQSLK DQALAQYLST SYARQASEIS LVSQVADQYL TLLSTDDLLK VTEDTLKTAQ ASYDLTKLQF DNGTGSELEL RQAQTVVETA LANQQAQARA RAQALNALVL LIGEPLPDDL PAGMPLDAQN LLTDVPAGLP SDLLTRRPDV MQAEQTLLAA NANIGAARAA FFPKISLTGA FGTASPTLGG LFKAGTAAWS FAPSIALPIF EGGQNIANLD LANVQKRIEI ANYEKAIQSA FREVSDGLAA RGTYDQQIAA LERNEHAQQR RFDLSDLRYK NGVDSYLSVL TAQTDLYAAQ QSLISARLAR WTNLVDLYRA LGGGWIQRAG ETPRAPDAPV DYQTATNG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgccgcac tacaagcggc ccgacgcacc cgtcgcgcag gcgtacccgg ccggcggcgt ctacgcgacg cagccgggcg cggccggcgc gcgcagcgcg aatggccagg cggcgactgc catcggctgg cgcgagttct tcgtcgatcc gcgcctgcag cggctgatcg agatcgcgct gaaaaacaac cgcgacctgc gcgtgtcggt gctcaatatc gaggccgcgc gtgcgcagta ccagatcacg cgtgcggggc tgttcccgac gctcgacggt acgggtacgg gcaaccgtca gcgtttgccg aattcgctga cggccgtgcc gggccggaac atcacgacga cctacaacgt cgggctgtcc gcgtcatggg aactcgacct gttcggccgc gtgcagagcc tgaaggacca ggcgctcgcg caatacctgt cgacgtcgta cgcgcgacag gcgtccgaga tctcgctggt gtcgcaggtc gcggatcaat atctgacgct gctgtcgacc gatgacctgc tgaaggtcac ggaggacacg ctgaagaccg cgcaggcatc gtacgacctg accaagctgc agttcgacaa cggcacgggc tccgagctcg agctgcgtca ggcgcagacg gtggtcgaga cggcgctcgc gaaccagcag gcgcaggcgc gagcccgcgc gcaggcgctg aacgcgctgg tgctgctgat cggcgagccg ctgccggacg atctgccggc gggcatgccg ctcgatgcgc agaacctgct gacggacgtg ccggccgggt tgccgtcgga tctgctgacg cgtcgtcctg acgtgatgca ggccgagcag acgctgctgg ccgcgaacgc caatatcggc gcggcgcgcg cggcgttctt cccgaagatt tcgctgacgg gcgcgttcgg caccgcgagc ccgacgctgg gcggcctgtt caaggccggc acggcggcgt ggtcgttcgc gccgagcatc gcgctgccga tcttcgaagg cgggcagaat atcgcgaacc tcgatctcgc gaacgtgcag aagcgcatcg agatcgcgaa ctacgagaag gcgatccagt cggcgttccg cgaagtgtcg gatggtctgg ccgcacgcgg cacgtacgac cagcagatcg cggcgctcga gcgcaacgag cacgcgcagc agcgacgctt cgatctgtcg gacctgcgct acaagaacgg tgtcgacagc tacctgtcgg tgctgaccgc gcagacggat ctgtacgccg cgcagcagtc gctgatcagc gcacgtctgg cacgctggac gaacctggtc gacctgtacc gcgcactggg cggcggctgg attcagcgcg ccggcgagac gccgcgcgcg ccggatgcgc cggtcgatta ccagacagcg acgaacggat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa

Details for BuceA.01673.v.AE2.GE43218

HARVESTED ON:	6/12/2019
SEQUENCED ON:	6/18/2019
EXPECTED MW:	52kDa
OBSERVED MW:	52kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	61
PERCENT COVERAGE:	100

Validated AA Sequence

MMAPHYKRPD APVAQAYPAG GVYATQPGAA GARSANGQAA TAIGWREFFV DPRLQRLIEI ALKNNRDLRV SVLNIEAARA QYQITRAGLF PTLDGTGTGN RQRLPNSLTA VPGRNITTTY NVGLSASWEL DLFGRVXSLK DXALAQYLST SYARQASXIS LVSQVADQYL TLLSTDXLLX VXEDXLKXAX ASYDLTKLQF DNGTGSXLEL RQXQTVVXTX LANQQAQARA RAQALNALVL LIGXPLPDDL XAGMPLDAXX LLXGRAGRVA VGSADASSXX DAXRADAAXR ERQYXRGARG VLPEDFADGR VRHREPDAGR PVQGRHGGVV VRXEHRAADL RRRAEYREPR SRERAEAHRD RELREGDPVG VPRSVGWSGR TRHVRPADRG ARAQRARAAA TXRSVGPALQ ERCRQLPVGA DRADGSVRRA AVADQRTSGT LDEPGRPVPR TGRRLDSARR RDAARAGCAG RLPDSDERSW APPSSSLT

Validated NT Sequence

tccgttaatg atgatgatgg tggtgcccag ctccgttcgt cgctgtctgg taatcgaccg gcgcatccgg cgcgcgcggc gtctcgccgg cgcgctgaat ccagccgccg cccagtgcgc ggtacaggtc gaccaggttc gtccagcgtg ccagacgtgc gctgatcagc gactgctgcg cggcgtacag atccgtctgc gcggtcagca ccgacnggta gctgtcgaca ccgttcttgt agcgcaggtc cgacagatcg aancgtcgct gctgcgcgtg ctcgttgcgc tcgagcgccg cgatctgctg gtcgtacgtg ccgcgtgcgg ccagaccatc cgacacttcg cggaacgccg actggatcgc cttctcgtag ttcgcgatct cgatgcgctt ctgcacgttc gcgagatcga ggttcgcgat attctgcccg ccttcgaaga tcggcagcgc gatgctcggn gcgaacgacc acgccgccgt gccggccttg aacaggncgc ccagcgtcgg gctcgcggtg ccgaacgcgc ccgtcngcga aatcttcggg aagaacgccg cgcgcgccgc gccnatattg gcgttcgcgg ncagcagcgt ctgctcggnc tgcatcnngn caggacgacg cgtcagcaga tccgacggca acccggccgg cacgtccnnt cagcagnntn tgcgcatcga gcggcatgcc cgccgncaga tcgtccggna gcggnncgcc gatcagcagc accagcgcgt tcagcgcctg cgcgcgggct cgcgcctgcg cctgctggtt cgcgagcncc gtcncgacca ccgtctgcnc ctgacgcagc tcgagctngg agcccgtgcc gttgtcgaac tgcagcttgg tcaggtcgta cgatgcntgc gcgntcttca gcgngtcctc cgngacnttc agcagntcat cggtcgacag cagcgtcaga tattgatccg cgacctgcga caccagcgag atntcggacg cctgtcgcgc gtacgacgtc gacaggtatt gcgcgagcgc ntggtccttc aggctntgca cgcggccgaa caggtcgagt tcccatgacg cggacagccc gacgttgtag gtcgtcgtga tgttccggcc cggcacggcc gtcagcgaat tcggcaaacg ctgacggttg cccgtacccg taccgtcgag cgtcgggaac agccccgcac gcgtgatctg gtactgcgca cgcgcggcct cgatattgag caccgacacg cgcaggtcgc ggttgttttt cagcgcgatc tcgatcagcc gctgcaggcg cggatcgacg aagaactcgc gccagccgat ggcagtcgcc gcctggccat tcgcgctgcg cgcgccggcc gcgcccggct gcgtcgcgta gacgccgccg gccgggtacg cctgcgcgac gggtgcgtcg ggccgcttgt agtgcggcgc catcatggga gaga

Expected Protein Sequence

MAPHYKRPDA PVAQAYPAGG VYATQPGAAG ARSANGQAAT AIGWREFFVD PRLQRLIEIA LKNNRDLRVS VLNIEAARAQ YQITRAGLFP TLDGTGTGNR QRLPNSLTAV PGRNITTTYN VGLSASWELD LFGRVQSLKD QALAQYLSTS YARQASEISL VSQVADQYLT LLSTDDLLKV TEDTLKTAQA SYDLTKLQFD NGTGSELELR QAQTVVETAL ANQQAQARAR AQALNALVLL IGEPLPDDLP AGMPLDAQNL LTDVPAGLPS DLLTRRPDVM QAEQTLLAAN ANIGAARAAF FPKISLTGAF GTASPTLGGL FKAGTAAWSF APSIALPIFE GGQNIANLDL ANVQKRIEIA NYEKAIQSAF REVSDGLAAR GTYDQQIAAL ERNEHAQQRR FDLSDLRYKN GVDSYLSVLT AQTDLYAAQQ SLISARLARW TNLVDLYRAL GGGWIQRAGE TPRAPDAPVD YQTATNGGHH HHHH

Full NT Sequence (Expression Vector + Insert)

tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atttgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag tctctcccat ggcgccgcac tacaagcggc ccgacgcacc cgtcgcgcag gcgtacccgg ccggcggcgt ctacgcgacg cagccgggcg cggccggcgc gcgcagcgcg aatggccagg cggcgactgc catcggctgg cgcgagttct tcgtcgatcc gcgcctgcag cggctgatcg agatcgcgct gaaaaacaac cgcgacctgc gcgtgtcggt gctcaatatc gaggccgcgc gtgcgcagta ccagatcacg cgtgcggggc tgttcccgac gctcgacggt acgggtacgg gcaaccgtca gcgtttgccg aattcgctga cggccgtgcc gggccggaac atcacgacga cctacaacgt cgggctgtcc gcgtcatggg aactcgacct gttcggccgc gtgcagagcc tgaaggacca ggcgctcgcg caatacctgt cgacgtcgta cgcgcgacag gcgtccgaga tctcgctggt gtcgcaggtc gcggatcaat atctgacgct gctgtcgacc gatgacctgc tgaaggtcac ggaggacacg ctgaagaccg cgcaggcatc gtacgacctg accaagctgc agttcgacaa cggcacgggc tccgagctcg agctgcgtca ggcgcagacg gtggtcgaga cggcgctcgc gaaccagcag gcgcaggcgc gagcccgcgc gcaggcgctg aacgcgctgg tgctgctgat cggcgagccg ctgccggacg atctgccggc gggcatgccg ctcgatgcgc agaacctgct gacggacgtg ccggccgggt tgccgtcgga tctgctgacg cgtcgtcctg acgtgatgca ggccgagcag acgctgctgg ccgcgaacgc caatatcggc gcggcgcgcg cggcgttctt cccgaagatt tcgctgacgg gcgcgttcgg caccgcgagc ccgacgctgg gcggcctgtt caaggccggc acggcggcgt ggtcgttcgc gccgagcatc gcgctgccga tcttcgaagg cgggcagaat atcgcgaacc tcgatctcgc gaacgtgcag aagcgcatcg agatcgcgaa ctacgagaag gcgatccagt cggcgttccg cgaagtgtcg gatggtctgg ccgcacgcgg cacgtacgac cagcagatcg cggcgctcga gcgcaacgag cacgcgcagc agcgacgctt cgatctgtcg gacctgcgct acaagaacgg tgtcgacagc tacctgtcgg tgctgaccgc gcagacggat ctgtacgccg cgcagcagtc gctgatcagc gcacgtctgg cacgctggac gaacctggtc gacctgtacc gcgcactggg cggcggctgg attcagcgcg ccggcgagac gccgcgcgcg ccggatgcgc cggtcgatta ccagacagcg acgaacggag ggcaccacca tcatcatcat taacggatcc gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggat

Details for BuceA.01673.v.A2.PW31765

PURIFICATION DATe:	5/4/2011
CONCENTRATION:	25.05mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	18
VIAL VOLUME:	200µl
PERCENT IDENTITY:	98
PERCENT COVERAGE:	87

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCCGCAC TACAAGCGGC CCGACGCACC CGTCGCGCAG GCGTACCCGG CCGGCGGCGT CTACGCGACG CAGCCGGGCG CGGCCGGCGC GCGCAGCGCG AATGGCCAGG CGGCGACTGC CATCGGCTGG CGCGAGTTCT TCGTCGATCC GCGCCTGCAG CGGCTGATCG AGATCGCGCT GAAAAACAAC CGCGACCTGC GCGTGTCGGT GCTCAATATC GAGGCCGCGC GTGCGCAGTA CCAGATCACG CGTGCGGGGC TGTTCCCGAC GCTCGACGGT ACGGGTACGG GCAACCGTCA GCGTTTGCCG AATTCGCTGA CGGCCGTGCC GGGCCGGAAC ATCACGACGA CCTACAACGT CGGGCTGTCC GCGTCATGGG AACTCGACCT GTTCGGCCGC GTGCAGAGCC TGAAGGACCA GGCGCTCGCG CAATACCTGT CGACGTCGTA CGCGCGACAG GCGTCCGAGA TCTCGCTGGT GTCGCAGGTC GCGGATCAAT ATCTGACGCT GCTGTCGACC GATGACCTGC TGAAGGTCAC GGAGGACACG CTGAAGACCG CGCAGGCATC GTACGACCTG ACCAAGCTGC AGTTCGACAA CGGCACGGGC TCCGAGCTCG AGCTGCGTCA GGCGCAGACG GTGGTCGAGA CGGCGCTCGC GAACCAGCAG GCGCAGGCGC GAGCCCGCGC GCAGGCGCTG AACGCGCTGG TGCTGCTGAT CGGCGAGCCG CTGCCGGACG ATCTGCCGGC GGGCATGCCG CTCGATGCGC AGAACCTGCT GACGGACGTG CCGGCCGGGT TGCCGTCGGA TCTGCTGACG CGTCGTCCTG ACGTGATGCA GGCCGAGCAG ACGCTGCTGG CCGCGAACGC CAATATCGGC GCGGCGCGCG CGGCGTTCTT CCCGAAGATT TCGCTGACGG GCGCGTTCGG CACCGCGAGC CCGACGCTGG GCGGCCTGTT CAAGGCCGGC ACGGCGGCGT GGTCGTTCGC GCCGAGCATC GCGCTGCCGA TCTTCGAAGG CGGGCAGAAT ATCGCGAACC TCGATCTCGC GAACGTGCAG AAGCGCATCG AGATCGCGAA CTACGAGAAG GCGATCCAGT CGGCGTTCCG CGAAGTGTCG GATGGTCTGG CCGCACGCGG CACGTACGAC CAGCAGATCG CGGCGCTCGA GCGCAACGAG CACGCGCAGC AGCGACGCTT CGATCTGTCG GACCTGCGCT ACAAGAACGG TGTCGACAGC TACCTGTCGG TGCTGACCGC GCAGACGGAT CTGTACGCCG CGCAGCAGTC GCTGATCAGC GCACGTCTGG CACGCTGGAC GAACCTGGTC GACCTGTACC GCGCACTGGG CGGCGGCTGG ATTCAGCGCG CCGGCGAGAC GCCGCGCGCG CCGGATGCGC CGGTCGATTA CCAGACAGCG ACGAACGGAT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA