BuceA.01673.u

Efflux system outer membrane protein

CENTER ID: BuceA.01673.u
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01673.u.A2.GE31851 Mature protein( BuceA.01673.u ) 19 475
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.7300
RefSeq: YP_002153975.1
UniProt: B4ENY7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRTLIALVC TGMLAGCTLD PTYQRPAAPV DNTWSTVPSR SQPKAAQSTA AAPLGADLGW RDFFKDPQLQ KLIELALVNN RDMRVAALNV AQYEAQYRIA RANVGPAINA SGSITRERVA GSTGAYGSSS ASVGLTSWEI DFFGRLRSLK RQALEQYLAT DAARTSTQIS LIATVATDYL QLRSDETSLQ IAQNTVDADQ HTYDLTVNMM KMGSASLQDV RQAENSLASA RSSLASYTRA VAQDRNNLVA EIGAPLPDDL PQGPSLMESE HTFADIGEGV PSDLLARRPD IVEAEHTLKA ANANIGAARA AFFPKIELTA SAGTTSGSLS NLFKAGTGAW TFAPTLSVPL FDFGYNRATL DVAKVEKDID VANYEKAIQT AFKEVSNALA GRTTYVTQVA ADRDYVESAR QYYDLAQARY RSGTDSFLTL LDAQRTLYAA QQQLVTDMLA KQSNLITLYK VLGGGWRETS AVAQR
NT Sequence
ATGAAACGCA CACTGATCGC CCTTGTCTGC ACGGGCATGC TGGCAGGCTG CACGCTGGAC CCGACGTACC AAAGGCCGGC CGCACCGGTC GACAATACCT GGTCCACCGT GCCCTCGCGG TCGCAGCCGA AGGCCGCGCA GAGCACGGCT GCGGCACCGC TGGGAGCCGA CCTCGGCTGG CGCGACTTCT TCAAGGATCC GCAACTGCAA AAGCTGATCG AACTCGCGCT CGTGAACAAC CGCGACATGC GCGTGGCCGC GCTCAACGTG GCGCAGTACG AAGCGCAGTA CCGCATTGCG CGCGCCAACG TCGGACCGGC CATCAACGCA TCCGGCTCGA TCACGCGCGA ACGCGTGGCC GGCTCCACGG GCGCATACGG TTCGAGCAGC GCGAGCGTGG GCCTCACATC GTGGGAAATC GACTTCTTCG GCCGCCTGCG CAGCCTCAAG CGCCAGGCGC TGGAGCAGTA CCTCGCCACG GACGCCGCGC GCACCAGCAC GCAGATCAGC CTGATCGCGA CCGTCGCCAC CGACTATCTG CAACTGCGCT CCGACGAGAC TTCGTTGCAG ATCGCGCAGA ACACCGTGGA TGCGGACCAG CACACCTACG ATCTCACGGT GAACATGATG AAAATGGGCA GCGCGTCGCT GCAGGACGTG CGCCAGGCCG AAAATTCGCT GGCGAGCGCG CGGTCGAGTC TCGCCTCGTA CACGCGTGCG GTCGCGCAGG ACCGCAACAA CCTGGTGGCC GAAATCGGTG CGCCGCTGCC CGACGATCTG CCGCAGGGCC CTTCGCTGAT GGAAAGCGAG CACACGTTCG CGGACATTGG CGAAGGCGTG CCGTCGGATC TGCTCGCGCG CCGGCCCGAC ATCGTCGAAG CCGAGCACAC GCTGAAGGCC GCCAACGCCA ACATCGGTGC GGCGCGCGCC GCGTTCTTTC CGAAGATCGA GCTGACGGCT TCCGCGGGCA CCACGAGCGG CAGCCTCTCG AACCTGTTCA AGGCGGGCAC GGGGGCGTGG ACGTTTGCGC CGACCCTCTC GGTGCCGCTC TTCGATTTCG GCTACAACCG CGCCACGCTG GACGTGGCGA AAGTCGAGAA GGACATCGAC GTCGCCAACT ATGAAAAGGC CATCCAGACG GCGTTCAAGG AGGTGTCGAA TGCACTCGCG GGACGCACCA CGTACGTCAC CCAGGTCGCC GCGGATCGCG ACTACGTCGA GTCCGCGCGG CAATACTACG ACCTCGCGCA GGCACGCTAC CGCAGCGGCA CCGACAGCTT CCTGACCCTG CTCGACGCGC AGCGCACGCT CTACGCCGCG CAACAGCAAC TGGTCACGGA CATGCTCGCC AAGCAGTCCA ATCTCATCAC GCTGTACAAG GTGCTGGGCG GCGGCTGGCG GGAAACGAGC GCGGTGGCGC AGCGG
Details for BuceA.01673.u.A2.GE31851
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 62
PERCENT COVERAGE: 83
Validated AA Sequence
MLDPTYQRPA APVDNTWSTV PSRSQPKAAQ STAAAPLGAD LGWRDFFKDP QLQKLIELAL VNNRDMRVAA LNVAQYEAQY RIARANVGPA INASGSITRE RVAGSTGAYG SSSASVGLTS WEIDFFGRLR SLKRQALEQY LATDAARTST QISLIATVAT DYLQLRSDET SLQIAQNTVD ADQHTYDLTV NMMKMXSASL QDVRQAENSL ASARSSLASY TRAXAXDRNX PGGRNRCAAA RRSAAGPFXX GKXXXXRXXL GEGVXSXXXS RXGPTSSXPX XTLKAANXQX RXRXXXXFFX XXRSXTXXXG XPXAXXPXXE XXXXRXGGGX XNXFXPXXXX XXXXXXXNXX XXXXXXXXXG XXXXXXXXXT PXXXXXXXXX XXXXXGXXXX XXXXXXXXX
Validated NT Sequence
gcnnnncagc catgtttann nnnactnctn tngnnnannn nnnnnnaann annannngnn nncnnnnnna gctcanacnn nnggtcctgg ttcgatgctg gacccgacgt accaaaggcc ggccgcaccg gtcgacaata cctggtccac cgtgccctcg cggtcgcagc cgaaggccgc gcagagcacg gctgcggcac cgctgggagc cgacctcggc tggcgcgact tcttcaagga tccgcaactg caaaagctga tcgaactcgc gctcgtgaac aaccgcgaca tgcgcgtggc cgcgctcaac gtggcgcagt acgaagcgca gtaccgcatt gcgcgcgcca acgtcggacc ggccatcaac gcatccggct cgatcacgcg cgaacgcgtg gccggctcca cgggcgcata cggttcgagc agcgcgagcg tgggcctcac atcgtgggaa atcgacttct tcggccgcct gcgcagcctc aagcgccagg cgctggagca gtacctcgcc acggacgccg cgcgcaccag cacgcagatc agcctgatcg cgaccgtcgc caccgactat ctgcaactgc gctccgacga gacttcgttg cagatcgcgc agaacaccgt ggatgcggac cagcacacct acgatctcac ggtgaacatg atgaaaatgn gcagcgcgtc gctgcaggac gtgcgccagg ccgaaaattc gctggcgagc gcgcggtcga gtctcgcctc gtacacgcgt gcggncgcgc angaccgcaa cnancctggt ggccgaaatc ggtgcgccgc tgcccgacga tctgccgcag ggcccttcnn nnnnggaaag nnagnnnnnn ttcgnnggan nttgggcgaa ggcgtgncgt cggnnncnng ctcgcgcgnc ggcccgacnt cntcgaancc naancanacg ctgaaggccg cnaacnncca anntcgggng cggnnnnnnn ccngnttctt tcnnaannat cgaagcnnna cgnnttncnn cgggcnncca nangcggnca nncctnncnn cgaancnnnn nttcnaaggg nngggggggg nnnnnngaac nnttttnnnc ccnanncccn nnnnnnggnn ngcnnnnnnn ttnnnaattt nngggnntnn nnnncnnnnn nccnnnngcn tggnnnnnnn nggnnnnaan nntnnngnna aaccccnnna nnntnnnnnn annnnnnnnt nnnnnnnnnn ngnnnnnnng gggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLDPTYQRP AAPVDNTWST VPSRSQPKAA QSTAAAPLGA DLGWRDFFKD PQLQKLIELA LVNNRDMRVA ALNVAQYEAQ YRIARANVGP AINASGSITR ERVAGSTGAY GSSSASVGLT SWEIDFFGRL RSLKRQALEQ YLATDAARTS TQISLIATVA TDYLQLRSDE TSLQIAQNTV DADQHTYDLT VNMMKMGSAS LQDVRQAENS LASARSSLAS YTRAVAQDRN NLVAEIGAPL PDDLPQGPSL MESEHTFADI GEGVPSDLLA RRPDIVEAEH TLKAANANIG AARAAFFPKI ELTASAGTTS GSLSNLFKAG TGAWTFAPTL SVPLFDFGYN RATLDVAKVE KDIDVANYEK AIQTAFKEVS NALAGRTTYV TQVAADRDYV ESARQYYDLA QARYRSGTDS FLTLLDAQRT LYAAQQQLVT DMLAKQSNLI TLYKVLGGGW RETSAVAQR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctggacccg acgtaccaaa ggccggccgc accggtcgac aatacctggt ccaccgtgcc ctcgcggtcg cagccgaagg ccgcgcagag cacggctgcg gcaccgctgg gagccgacct cggctggcgc gacttcttca aggatccgca actgcaaaag ctgatcgaac tcgcgctcgt gaacaaccgc gacatgcgcg tggccgcgct caacgtggcg cagtacgaag cgcagtaccg cattgcgcgc gccaacgtcg gaccggccat caacgcatcc ggctcgatca cgcgcgaacg cgtggccggc tccacgggcg catacggttc gagcagcgcg agcgtgggcc tcacatcgtg ggaaatcgac ttcttcggcc gcctgcgcag cctcaagcgc caggcgctgg agcagtacct cgccacggac gccgcgcgca ccagcacgca gatcagcctg atcgcgaccg tcgccaccga ctatctgcaa ctgcgctccg acgagacttc gttgcagatc gcgcagaaca ccgtggatgc ggaccagcac acctacgatc tcacggtgaa catgatgaaa atgggcagcg cgtcgctgca ggacgtgcgc caggccgaaa attcgctggc gagcgcgcgg tcgagtctcg cctcgtacac gcgtgcggtc gcgcaggacc gcaacaacct ggtggccgaa atcggtgcgc cgctgcccga cgatctgccg cagggccctt cgctgatgga aagcgagcac acgttcgcgg acattggcga aggcgtgccg tcggatctgc tcgcgcgccg gcccgacatc gtcgaagccg agcacacgct gaaggccgcc aacgccaaca tcggtgcggc gcgcgccgcg ttctttccga agatcgagct gacggcttcc gcgggcacca cgagcggcag cctctcgaac ctgttcaagg cgggcacggg ggcgtggacg tttgcgccga ccctctcggt gccgctcttc gatttcggct acaaccgcgc cacgctggac gtggcgaaag tcgagaagga catcgacgtc gccaactatg aaaaggccat ccagacggcg ttcaaggagg tgtcgaatgc actcgcggga cgcaccacgt acgtcaccca ggtcgccgcg gatcgcgact acgtcgagtc cgcgcggcaa tactacgacc tcgcgcaggc acgctaccgc agcggcaccg acagcttcct gaccctgctc gacgcgcagc gcacgctcta cgccgcgcaa cagcaactgg tcacggacat gctcgccaag cagtccaatc tcatcacgct gtacaaggtg ctgggcggcg gctggcggga aacgagcgcg gtggcgcagc ggtaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa