BuceA.01673.t

Efflux system outer membrane protein

CENTER ID: BuceA.01673.t
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01673.t.A2.GE34652 Mature protein( BuceA.01673.t ) 37 513
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.7289
RefSeq: YP_002153968.1
UniProt: B4ENY0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRLPHPLPFA RRIVALGAVA ALTACSTLPR YSPPAVAVPA HYAGAPAAPA EQTGWHVAMP ADAAARGAWW TVFDDADLNA LEARVDVSNQ TVKKAVADLQ QARAMVDYQH AGFLPTITAG AAQSRARVSQ NRLGSSLAGK TTPDYQVGVA ASWEPDLFGR VRDAVTGAQA NAAASAADLQ AVKLSVTAEL ATDYFALRSL DTQKQLLDDT VHAYADALTL LKQQLAAGAI DASAVAQAET QLESTRTQDT DIDASRAQLQ HAIATLVGES ASTFALPPHV QPFHVPAIPA GVPSQLLERR PDIAAAERRV AAANAQIGEA RAAFFPDLVL SASAGLESSF FAPWLAAPSL FWSLGPQLAG TLFDGGRRSA SLRGAHAQYD GAVADYRQTV LVAFQQVEDQ LSALDALASE AGSQQRATDA ADLSLRLTTN RFNAGAVSYL DVVTAQTIAL TNRRTADQID ARRIEAEVGL LKALGGGWQV SVSAGSDAES NTDTHEHAAA ARHEPAVTQP AAG
NT Sequence
ATGCGTCTTC CTCATCCGCT CCCGTTCGCT CGCCGCATCG TCGCGCTCGG CGCCGTCGCC GCACTGACCG CCTGCTCGAC GCTGCCGCGC TATTCGCCGC CCGCCGTCGC GGTGCCCGCG CACTATGCAG GCGCACCGGC AGCACCTGCC GAACAAACCG GCTGGCACGT CGCGATGCCG GCCGACGCCG CCGCGCGCGG CGCCTGGTGG ACTGTCTTCG ACGATGCCGA TCTGAACGCG CTCGAAGCGC GCGTCGACGT GTCGAACCAG ACGGTGAAGA AAGCCGTCGC CGATCTGCAG CAAGCCCGCG CGATGGTCGA CTATCAGCAC GCCGGGTTCC TGCCGACGAT CACGGCCGGT GCCGCGCAGA GCCGGGCGCG CGTGTCGCAG AACCGGCTCG GCTCGTCGCT CGCCGGCAAG ACGACGCCCG ACTACCAGGT CGGCGTGGCC GCGAGCTGGG AGCCCGACCT GTTCGGCCGC GTGCGCGACG CGGTGACCGG CGCGCAGGCG AACGCCGCCG CGAGCGCGGC CGACCTGCAG GCCGTGAAGC TGTCGGTCAC GGCCGAACTC GCGACCGACT ATTTCGCGCT GCGCTCGCTC GACACGCAGA AGCAACTGCT CGACGACACG GTGCATGCGT ACGCGGACGC GCTGACGCTG CTCAAGCAGC AACTCGCGGC CGGTGCGATC GACGCGTCGG CGGTCGCGCA AGCCGAGACG CAGCTCGAAT CGACCCGCAC GCAGGACACC GACATCGATG CGTCGCGTGC GCAGTTGCAG CACGCGATCG CGACGCTCGT CGGCGAAAGC GCGTCGACGT TCGCGCTGCC GCCGCACGTA CAGCCGTTCC ACGTGCCGGC CATTCCGGCC GGCGTGCCGT CGCAACTGCT GGAGCGGCGG CCCGACATCG CGGCGGCCGA GCGTCGCGTC GCGGCCGCGA ACGCGCAGAT CGGCGAGGCG CGGGCCGCGT TCTTCCCGGA TCTCGTGCTG TCGGCGAGCG CCGGCCTCGA AAGTTCGTTC TTCGCGCCGT GGCTCGCCGC GCCGAGCCTG TTCTGGTCGC TCGGCCCGCA ACTCGCCGGC ACGCTGTTCG ACGGCGGCCG CCGCAGCGCG TCGCTGCGCG GCGCGCATGC GCAATACGAC GGTGCGGTCG CCGACTACCG GCAAACCGTG CTCGTCGCGT TCCAGCAGGT CGAGGATCAG TTGTCCGCGC TCGATGCGCT CGCGTCCGAG GCCGGCAGCC AGCAGCGCGC GACCGACGCG GCCGACCTGT CACTGCGGCT GACGACGAAT CGCTTCAACG CGGGCGCCGT CAGCTATCTG GACGTGGTGA CCGCGCAGAC GATCGCGCTG ACGAACCGGC GCACGGCCGA TCAGATCGAC GCGCGCCGGA TCGAGGCCGA AGTCGGGTTG CTGAAGGCGC TGGGTGGTGG CTGGCAGGTA AGCGTAAGCG CCGGCTCAGA CGCGGAATCG AACACCGACA CGCACGAACA CGCGGCCGCC GCGCGCCACG AACCCGCCGT CACGCAGCCG GCGGCCGGT
Details for BuceA.01673.t.A2.GE34652
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 83
PERCENT COVERAGE: 76
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAVPAHYAG APAAPAEQTG WHVAMPADAA ARGAWWTVFD DADLNALEAR VDVSNQTVKK AVADLQQARA MVDYQHAGFL PTITAGAAQS RARVSQNRLG SSLAGKTTPD YQVGVAASWE PDLFGRVRDA VTGAQANAAA SAADLQAVKL SVTAELATDY FALRSLDTQK QLLDDTVHAY ADALTLLKQQ LAAGAIDASA VAQAETQLES TRTQDTDIDA SRAQLQHAIA TLVGESASTF ALPPHVQPFH VPAIPAGVPS QLLERRPDIA AAERRVAAAN AQIGEARAXF FPDLXLXASA XXXKFXLRXX XXXXPXXXAX XXXXXXXSXX XXXXLXXXHX XXXXXXXXXX XXXXXXXXXX
Validated NT Sequence
attttgtnnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggcgg tgcccgcgca ctatgcaggc gcaccggcag cacctgccga acaaaccggc tggcacgtcg cgatgccggc cgacgccgcc gcgcgcggcg cctggtggac tgtcttcgac gatgccgatc tgaacgcgct cgaagcgcgc gtcgacgtgt cgaaccagac ggtgaagaaa gccgtcgccg atctgcagca agcccgcgcg atggtcgact atcagcacgc cgggttcctg ccgacgatca cggccggtgc cgcgcagagc cgggcgcgcg tgtcgcagaa ccggctcggc tcgtcgctcg ccggcaagac gacgcccgac taccaggtcg gcgtggccgc gagctgggag cccgacctgt tcggccgcgt gcgcgacgcg gtgaccggcg cgcaggcgaa cgccgccgcg agcgcggccg acctgcaggc cgtgaagctg tcggtcacgg ccgaactcgc gaccgactat ttcgcgctgc gctcgctcga cacgcagaag caactgctcg acgacacggt gcatgcgtac gcggacgcgc tgacgctgct caagcagcaa ctcgcggccg gtgcgatcga cgcgtcggcg gtcgcgcaag ccgagacgca gctcgaatcg acccgcacgc aggacaccga catcgatgcg tcgcgtgcgc agttgcagca cgcgatcgcg acgctcgtcg gcgaaagcgc gtcgacgttc gcgctgccgc cgcacgtaca gccgttccac gtgccggcca ttccggccgg cgtgccgtcg caactgctgg agcggcggcc cgacatcgcg gcggccgagc gtcgcgtcgc ggccgcgaac gcgcagatcg gcgaggcgcg ggcnncgttc ttcccggatc tcgngctgtn ggcnagcgcc ngncntcnaa agttcnttct tcgcnncnnn nncnnnnnnc anccnnnnnn nnnngctnng nnnnnantcn nnnnnnnctn ntcgangnnn nnnannnnnn cnctnnnnnn nnnncatnnn nanncnnnng nngnnnnnnt nnnnnnannn nnnnnnntnn nnnnnnnnnn nnnnnngnnn naa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAVPAHYAG APAAPAEQTG WHVAMPADAA ARGAWWTVFD DADLNALEAR VDVSNQTVKK AVADLQQARA MVDYQHAGFL PTITAGAAQS RARVSQNRLG SSLAGKTTPD YQVGVAASWE PDLFGRVRDA VTGAQANAAA SAADLQAVKL SVTAELATDY FALRSLDTQK QLLDDTVHAY ADALTLLKQQ LAAGAIDASA VAQAETQLES TRTQDTDIDA SRAQLQHAIA TLVGESASTF ALPPHVQPFH VPAIPAGVPS QLLERRPDIA AAERRVAAAN AQIGEARAAF FPDLVLSASA GLESSFFAPW LAAPSLFWSL GPQLAGTLFD GGRRSASLRG AHAQYDGAVA DYRQTVLVAF QQVEDQLSAL DALASEAGSQ QRATDAADLS LRLTTNRFNA GAVSYLDVVT AQTIALTNRR TADQIDARRI EAEVGLLKAL GGGWQVSVSA GSDAESNTDT HEHAAAARHE PAVTQPAAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcggtgccc gcgcactatg caggcgcacc ggcagcacct gccgaacaaa ccggctggca cgtcgcgatg ccggccgacg ccgccgcgcg cggcgcctgg tggactgtct tcgacgatgc cgatctgaac gcgctcgaag cgcgcgtcga cgtgtcgaac cagacggtga agaaagccgt cgccgatctg cagcaagccc gcgcgatggt cgactatcag cacgccgggt tcctgccgac gatcacggcc ggtgccgcgc agagccgggc gcgcgtgtcg cagaaccggc tcggctcgtc gctcgccggc aagacgacgc ccgactacca ggtcggcgtg gccgcgagct gggagcccga cctgttcggc cgcgtgcgcg acgcggtgac cggcgcgcag gcgaacgccg ccgcgagcgc ggccgacctg caggccgtga agctgtcggt cacggccgaa ctcgcgaccg actatttcgc gctgcgctcg ctcgacacgc agaagcaact gctcgacgac acggtgcatg cgtacgcgga cgcgctgacg ctgctcaagc agcaactcgc ggccggtgcg atcgacgcgt cggcggtcgc gcaagccgag acgcagctcg aatcgacccg cacgcaggac accgacatcg atgcgtcgcg tgcgcagttg cagcacgcga tcgcgacgct cgtcggcgaa agcgcgtcga cgttcgcgct gccgccgcac gtacagccgt tccacgtgcc ggccattccg gccggcgtgc cgtcgcaact gctggagcgg cggcccgaca tcgcggcggc cgagcgtcgc gtcgcggccg cgaacgcgca gatcggcgag gcgcgggccg cgttcttccc ggatctcgtg ctgtcggcga gcgccggcct cgaaagttcg ttcttcgcgc cgtggctcgc cgcgccgagc ctgttctggt cgctcggccc gcaactcgcc ggcacgctgt tcgacggcgg ccgccgcagc gcgtcgctgc gcggcgcgca tgcgcaatac gacggtgcgg tcgccgacta ccggcaaacc gtgctcgtcg cgttccagca ggtcgaggat cagttgtccg cgctcgatgc gctcgcgtcc gaggccggca gccagcagcg cgcgaccgac gcggccgacc tgtcactgcg gctgacgacg aatcgcttca acgcgggcgc cgtcagctat ctggacgtgg tgaccgcgca gacgatcgcg ctgacgaacc ggcgcacggc cgatcagatc gacgcgcgcc ggatcgaggc cgaagtcggg ttgctgaagg cgctgggtgg tggctggcag gtaagcgtaa gcgccggctc agacgcggaa tcgaacaccg acacgcacga acacgcggcc gccgcgcgcc acgaacccgc cgtcacgcag ccggcggccg gttaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa