SSGCID: Target Page

BuceA.01673.r

Efflux system outer membrane protein

CENTER ID:	BuceA.01673.r
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01673.r.A2.GE34655	Mature protein( BuceA.01673.r )		19	507

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01673.r.A2.PW35265	Mature protein( BuceA.01673.r )		19	507

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.5704
RefSeq:	YP_002234553.1
UniProt:	B4EMS8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAAFRLSAL AMSVTLAACA VGPDFKRPDT ATAARFARDA HPATQHDAAP ADTAPETPPD ASAAADATFW RGFGDPTLTQ LIDAALAANQ DLQVAVSRYD ASNALLSQAT FDRYPTITAS GQIGHRLMSK DQAFGAPRSQ RDAPTSSIGI NAAWELDLFG RVRRSIESQR AETAASAADV RAVRVAIAGE VASTYVDLRG SQERLRIARE NADNQKQTLA LINARVGAGR GSELDAARAR AQYESTTSRI AVYEAAIGVD EHRLAVLTGQ TPDALIGRFD LPGTAASTGT PVLLPALAAD IDPGAPGELL RRRPDVAAAE ARLHAATARV GVATADLFPR FTLSGLLGSA TSSYGFFRAG SDTNLIALGI DWSFLDVGRV RARIAASDAE AAGQLAQYRQ TVLGALEETE NALLRVARTR DETAHLVRAA DDSARAAQLA QARFSAGAID YYEVLDAQRT LLQAQDAAAD GRMRSAASTV ALYKALAGGW PSGTGQGAPG PLARSGQ

NT Sequence

ATGAGCGCCG CGTTCCGTCT TTCCGCGCTC GCGATGTCGG TGACGCTCGC CGCATGCGCG GTGGGTCCCG ATTTCAAACG GCCCGACACC GCCACCGCCG CGCGGTTCGC GCGCGACGCC CACCCGGCGA CGCAGCACGA TGCGGCGCCG GCCGATACGG CGCCCGAGAC GCCGCCCGAC GCGTCGGCTG CCGCCGATGC CACGTTCTGG CGCGGGTTCG GCGATCCGAC GCTGACGCAG TTGATCGATG CCGCGCTCGC CGCGAACCAG GACCTGCAGG TCGCCGTATC GCGCTACGAC GCATCGAATG CGCTGCTGTC GCAGGCCACG TTCGACCGCT ATCCGACGAT TACCGCGAGC GGCCAGATCG GCCATCGGCT GATGAGCAAG GACCAGGCGT TCGGTGCGCC GCGCAGCCAG CGCGATGCGC CGACGTCGAG CATCGGCATC AATGCCGCGT GGGAACTCGA CCTGTTCGGC CGGGTGCGCC GCTCGATCGA GTCGCAGCGC GCGGAAACGG CGGCCAGCGC GGCCGACGTG CGCGCGGTGC GCGTCGCGAT CGCGGGCGAG GTCGCGAGCA CGTACGTCGA TCTGCGCGGC TCGCAGGAGC GCTTGCGGAT CGCCCGCGAG AACGCCGACA ACCAGAAGCA GACGCTCGCG CTGATCAATG CGCGCGTCGG CGCGGGACGC GGCTCCGAAC TGGATGCGGC GCGCGCGCGT GCGCAGTACG AATCGACGAC CTCGCGGATC GCCGTGTACG AAGCCGCGAT CGGCGTCGAC GAGCATCGGC TCGCGGTGCT GACCGGGCAG ACGCCGGATG CGCTGATCGG CCGGTTCGAC CTGCCGGGGA CGGCCGCGTC GACCGGCACG CCGGTGCTGC TGCCGGCGCT CGCGGCCGAC ATCGATCCCG GCGCGCCGGG CGAACTGCTG CGCCGCCGGC CCGATGTCGC GGCCGCCGAG GCGCGGCTGC ATGCGGCGAC CGCGCGCGTC GGCGTCGCGA CCGCCGACCT GTTCCCGCGC TTTACGCTGT CGGGGCTGCT CGGCAGCGCG ACGAGCAGCT ACGGGTTCTT TCGCGCGGGC AGCGACACGA ACCTGATCGC GCTCGGCATC GACTGGTCGT TCCTCGACGT CGGCCGCGTG CGCGCACGGA TCGCCGCGAG CGACGCGGAA GCGGCCGGCC AGCTTGCGCA GTACCGGCAG ACCGTGCTCG GCGCGCTGGA GGAAACCGAG AACGCGCTGC TGCGTGTCGC CCGCACGCGC GACGAGACCG CGCACCTCGT GCGCGCGGCC GACGACAGCG CGCGCGCCGC GCAGCTCGCG CAGGCCCGGT TCTCGGCCGG CGCGATCGAC TACTACGAAG TGCTCGACGC GCAGCGGACG CTGCTGCAGG CGCAGGACGC GGCGGCCGAC GGGCGGATGC GCAGCGCGGC ATCGACGGTC GCGCTGTACA AGGCGCTCGC GGGCGGCTGG CCGTCGGGCA CGGGGCAGGG CGCGCCCGGT CCGCTCGCGC GGTCGGGGCA G

Details for BuceA.01673.r.A2.GE34655

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	53kDa
OBSERVED MW:	53kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	53

Validated AA Sequence

MGTLEAQTQG PGSMCAVGPD FKRPDTATAA RFARDAHPAT QHDAAPADTA PETPPDASAA ADATFWRGFG DPTLTQLIDA ALAANQDLQV AVSRYDASNA LLSQATFDRY PTITASGQIG HRLMSKDQAF GAPRSQRDAP TSSIGINAAW ELDLFGRVRR SIESQRAETA ASAADVRAVR VAIAGEVAST YVDLRGSQER LRIARENADX QKQTLALINA RVGAGRGSEL DAARARAQYE STTSRIAVYE AAIGVDEHRL AVLTGQTPDA LI

Validated NT Sequence

tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgtgcgc ggtgggtccc gatttcaaac ggcccgacac cgccaccgcc gcgcggttcg cgcgcgacgc ccacccggcg acgcagcacg atgcggcgcc ggccgatacg gcgcccgaga cgccgcccga cgcgtcggct gccgccgatg ccacgttctg gcgcgggttc ggcgatccga cgctgacgca gttgatcgat gccgcgctcg ccgcgaacca ggacctgcag gtcgccgtat cgcgctacga cgcatcgaat gcgctgctgt cgcaggccac gttcgaccgc tatccgacga ttaccgcgag cggccagatc ggccatcggc tgatgagcaa ggaccaggcg ttcggtgcgc cgcgcagcca gcgcgatgcg ccgacgtcga gcatcggcat caatgccgcg tgggaactcg acctgttcgg ccgggtgcgc cgctcgatcg agtcgcagcg cgcggaaacg gcggccagcg cggccgacgt gcgcgcggtg cgcgtcgcga tcgcgggcga ggtcgcgagc acgtacgtcg atctgcgcgg ctcgcaggag cgcttgcgga tcgcccgcga gaacgccgac naccagaagc agacgctcgc gctgatcaat gcgcgcgtcg gcgcgggacg cggctccgaa ctggatgcgg cgcgcgcgcg tgcgcagtac gaatcgacga cctcgcggat cgccgtgtac gaagccgcga tcggcgtcga cgagcatcgg ctcgcggtgc tgaccgggca gacgccngat gcgctgatcg g

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMCAVGPDFK RPDTATAARF ARDAHPATQH DAAPADTAPE TPPDASAAAD ATFWRGFGDP TLTQLIDAAL AANQDLQVAV SRYDASNALL SQATFDRYPT ITASGQIGHR LMSKDQAFGA PRSQRDAPTS SIGINAAWEL DLFGRVRRSI ESQRAETAAS AADVRAVRVA IAGEVASTYV DLRGSQERLR IARENADNQK QTLALINARV GAGRGSELDA ARARAQYEST TSRIAVYEAA IGVDEHRLAV LTGQTPDALI GRFDLPGTAA STGTPVLLPA LAADIDPGAP GELLRRRPDV AAAEARLHAA TARVGVATAD LFPRFTLSGL LGSATSSYGF FRAGSDTNLI ALGIDWSFLD VGRVRARIAA SDAEAAGQLA QYRQTVLGAL EETENALLRV ARTRDETAHL VRAADDSARA AQLAQARFSA GAIDYYEVLD AQRTLLQAQD AAADGRMRSA ASTVALYKAL AGGWPSGTGQ GAPGPLARSG Q

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcgcggtg ggtcccgatt tcaaacggcc cgacaccgcc accgccgcgc ggttcgcgcg cgacgcccac ccggcgacgc agcacgatgc ggcgccggcc gatacggcgc ccgagacgcc gcccgacgcg tcggctgccg ccgatgccac gttctggcgc gggttcggcg atccgacgct gacgcagttg atcgatgccg cgctcgccgc gaaccaggac ctgcaggtcg ccgtatcgcg ctacgacgca tcgaatgcgc tgctgtcgca ggccacgttc gaccgctatc cgacgattac cgcgagcggc cagatcggcc atcggctgat gagcaaggac caggcgttcg gtgcgccgcg cagccagcgc gatgcgccga cgtcgagcat cggcatcaat gccgcgtggg aactcgacct gttcggccgg gtgcgccgct cgatcgagtc gcagcgcgcg gaaacggcgg ccagcgcggc cgacgtgcgc gcggtgcgcg tcgcgatcgc gggcgaggtc gcgagcacgt acgtcgatct gcgcggctcg caggagcgct tgcggatcgc ccgcgagaac gccgacaacc agaagcagac gctcgcgctg atcaatgcgc gcgtcggcgc gggacgcggc tccgaactgg atgcggcgcg cgcgcgtgcg cagtacgaat cgacgacctc gcggatcgcc gtgtacgaag ccgcgatcgg cgtcgacgag catcggctcg cggtgctgac cgggcagacg ccggatgcgc tgatcggccg gttcgacctg ccggggacgg ccgcgtcgac cggcacgccg gtgctgctgc cggcgctcgc ggccgacatc gatcccggcg cgccgggcga actgctgcgc cgccggcccg atgtcgcggc cgccgaggcg cggctgcatg cggcgaccgc gcgcgtcggc gtcgcgaccg ccgacctgtt cccgcgcttt acgctgtcgg ggctgctcgg cagcgcgacg agcagctacg ggttctttcg cgcgggcagc gacacgaacc tgatcgcgct cggcatcgac tggtcgttcc tcgacgtcgg ccgcgtgcgc gcacggatcg ccgcgagcga cgcggaagcg gccggccagc ttgcgcagta ccggcagacc gtgctcggcg cgctggagga aaccgagaac gcgctgctgc gtgtcgcccg cacgcgcgac gagaccgcgc acctcgtgcg cgcggccgac gacagcgcgc gcgccgcgca gctcgcgcag gcccggttct cggccggcgc gatcgactac tacgaagtgc tcgacgcgca gcggacgctg ctgcaggcgc aggacgcggc ggccgacggg cggatgcgca gcgcggcatc gacggtcgcg ctgtacaagg cgctcgcggg cggctggccg tcgggcacgg ggcagggcgc gcccggtccg ctcgcgcggt cggggcagta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a

Details for BuceA.01673.r.A2.PW35265

PURIFICATION DATe:	5/17/2012
CONCENTRATION:	7.66mg/ml
OBSERVED MW:	56kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	4
VIAL VOLUME:	110µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	53

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTGCGCGGTG GGTCCCGATT TCAAACGGCC CGACACCGCC ACCGCCGCGC GGTTCGCGCG CGACGCCCAC CCGGCGACGC AGCACGATGC GGCGCCGGCC GATACGGCGC CCGAGACGCC GCCCGACGCG TCGGCTGCCG CCGATGCCAC GTTCTGGCGC GGGTTCGGCG ATCCGACGCT GACGCAGTTG ATCGATGCCG CGCTCGCCGC GAACCAGGAC CTGCAGGTCG CCGTATCGCG CTACGACGCA TCGAATGCGC TGCTGTCGCA GGCCACGTTC GACCGCTATC CGACGATTAC CGCGAGCGGC CAGATCGGCC ATCGGCTGAT GAGCAAGGAC CAGGCGTTCG GTGCGCCGCG CAGCCAGCGC GATGCGCCGA CGTCGAGCAT CGGCATCAAT GCCGCGTGGG AACTCGACCT GTTCGGCCGG GTGCGCCGCT CGATCGAGTC GCAGCGCGCG GAAACGGCGG CCAGCGCGGC CGACGTGCGC GCGGTGCGCG TCGCGATCGC GGGCGAGGTC GCGAGCACGT ACGTCGATCT GCGCGGCTCG CAGGAGCGCT TGCGGATCGC CCGCGAGAAC GCCGACAACC AGAAGCAGAC GCTCGCGCTG ATCAATGCGC GCGTCGGCGC GGGACGCGGC TCCGAACTGG ATGCGGCGCG CGCGCGTGCG CAGTACGAAT CGACGACCTC GCGGATCGCC GTGTACGAAG CCGCGATCGG CGTCGACGAG CATCGGCTCG CGGTGCTGAC CGGGCAGACG CCGGATGCGC TGATCGGCCG GTTCGACCTG CCGGGGACGG CCGCGTCGAC CGGCACGCCG GTGCTGCTGC CGGCGCTCGC GGCCGACATC GATCCCGGCG CGCCGGGCGA ACTGCTGCGC CGCCGGCCCG ATGTCGCGGC CGCCGAGGCG CGGCTGCATG CGGCGACCGC GCGCGTCGGC GTCGCGACCG CCGACCTGTT CCCGCGCTTT ACGCTGTCGG GGCTGCTCGG CAGCGCGACG AGCAGCTACG GGTTCTTTCG CGCGGGCAGC GACACGAACC TGATCGCGCT CGGCATCGAC TGGTCGTTCC TCGACGTCGG CCGCGTGCGC GCACGGATCG CCGCGAGCGA CGCGGAAGCG GCCGGCCAGC TTGCGCAGTA CCGGCAGACC GTGCTCGGCG CGCTGGAGGA AACCGAGAAC GCGCTGCTGC GTGTCGCCCG CACGCGCGAC GAGACCGCGC ACCTCGTGCG CGCGGCCGAC GACAGCGCGC GCGCCGCGCA GCTCGCGCAG GCCCGGTTCT CGGCCGGCGC GATCGACTAC TACGAAGTGC TCGACGCGCA GCGGACGCTG CTGCAGGCGC AGGACGCGGC GGCCGACGGG CGGATGCGCA GCGCGGCATC GACGGTCGCG CTGTACAAGG CGCTCGCGGG CGGCTGGCCG TCGGGCACGG GGCAGGGCGC GCCCGGTCCG CTCGCGCGGT CGGGGCAGTA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A