BuceA.01673.q

Outer membrane efflux protein

CENTER ID: BuceA.01673.q
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01673.q.A2.GE31847 Mature protein( BuceA.01673.q ) 21 480
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.4499
RefSeq: YP_002233410.1
UniProt: B4EMJ2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKLLVALAV SAFAAGCAVQ PAQHPALNES VQTLAPTVWD TDVPRADVDA AAWWAQFHDP VLDRLIATVL DGNLDLQAAA ERVKQAQALT VQKRAVLLPE LDATAHAADA RQNTPPPLGY VRQAGAGLAL SWSPDVFGGE RLDLLAAQAE LVGQKHAEDA MRLALAADAA SAYVDLRWAQ QELKILQDNA KIRQHALELT RKRQAFGLST ELDVTRAQNQ LDALEARIPP TQAQISHQLN LIAVYSGRTP ESVDRLVLAQ AGEIPAPPIG APGTLPSQAL LRRPDVLTAY AQVERRAAQV GVAKAERYPK FSLNLTDGIL AASYLGLPTL TDNLFSAALS ATSPIFNAGR ITADISQSES RMRESELGLR QTMLQALREV EDARANLVSS DEATRRLGSA LAASDKALGL ANQLYKGGAT DFLDVLSAQE VYLRDSDSLN QVKREHALAA VALYRSLGGG WSRNEAAGGP RAETEAVAKN
NT Sequence
ATGAAGAAGC TACTCGTCGC GCTGGCCGTC AGCGCATTCG CCGCCGGGTG CGCGGTGCAG CCCGCGCAGC ATCCGGCGCT GAACGAATCC GTTCAGACGC TCGCGCCCAC CGTGTGGGAT ACCGACGTGC CGCGCGCGGA CGTCGATGCG GCCGCCTGGT GGGCGCAGTT CCACGACCCC GTGCTCGACC GGCTGATCGC GACCGTGCTC GACGGGAACC TCGATCTGCA AGCCGCCGCC GAACGCGTGA AGCAGGCGCA GGCGCTGACC GTGCAGAAAC GCGCGGTGCT GCTGCCCGAA CTCGACGCGA CCGCGCATGC GGCCGACGCG CGGCAGAACA CGCCGCCGCC GCTCGGTTAT GTGCGGCAGG CTGGGGCCGG GCTCGCGCTG AGCTGGTCGC CCGATGTGTT CGGCGGTGAA CGGCTCGATT TGCTGGCCGC TCAAGCGGAG TTGGTCGGGC AAAAGCACGC GGAAGATGCG ATGCGCCTTG CGCTTGCCGC CGATGCGGCA TCCGCGTATG TCGATCTGCG CTGGGCGCAG CAGGAACTGA AGATCCTGCA GGACAACGCG AAGATCCGGC AGCATGCGCT CGAACTCACA CGCAAGCGGC AGGCGTTCGG GCTGTCGACG GAACTCGACG TGACGCGTGC GCAGAACCAG CTCGACGCGC TCGAGGCGCG GATTCCGCCG ACGCAGGCGC AGATTTCGCA TCAGCTCAAT CTGATCGCCG TGTATTCGGG GCGGACGCCG GAATCGGTCG ATCGGCTCGT GCTGGCGCAG GCCGGGGAGA TTCCCGCGCC GCCGATTGGC GCGCCGGGGA CGCTGCCTTC GCAGGCGTTG TTGCGGCGGC CGGATGTGCT GACTGCCTAT GCGCAGGTCG AGCGACGGGC AGCGCAAGTG GGCGTGGCAA AGGCCGAGCG GTATCCGAAG TTTTCGTTGA ACCTGACGGA CGGGATTCTG GCGGCTTCCT ATCTCGGGCT GCCGACGTTG ACCGATAACC TGTTCAGCGC GGCACTGAGC GCGACGAGCC CGATCTTCAA CGCGGGGCGG ATTACCGCCG ATATCTCGCA GAGCGAAAGC CGGATGCGCG AGTCGGAACT CGGGTTACGG CAGACGATGC TGCAAGCACT GCGGGAAGTC GAGGATGCGC GTGCGAATCT CGTCAGTTCC GATGAGGCGA CGCGAAGGCT GGGTAGCGCA TTGGCGGCTT CCGATAAAGC GCTCGGGCTG GCGAATCAGC TCTATAAGGG CGGCGCGACG GATTTTCTGG ATGTGCTGTC GGCGCAGGAG GTTTATCTGC GGGATTCGGA TTCGCTCAAT CAGGTCAAGC GCGAACATGC GCTCGCGGCG GTGGCGTTGT ATCGGTCACT TGGGGGTGGG TGGAGCAGGA ATGAGGCGGC GGGTGGGCCG CGGGCGGAAA CCGAAGCTGT GGCAAAGAAC
Details for BuceA.01673.q.A2.GE31847
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 96
PERCENT COVERAGE: 53
Validated AA Sequence
MRXALAXDAX SAYVDLRWAQ QXLKXXQDNA KXRQHALEXT RXRXAFGLST ELDVTRAQNQ LDALEARIPP TQAQISHQLN LIAVYSGRTP ESVDRLVLAQ AGEIPAPPIG APGTLPSQAL LRRPDVLTAY AQVERRAAQV GVAKAERYPK FSLNLTDGIL AASYLGLPTL TDNLFSAALS ATSPIFNAGR ITADISQSES RMRESELGLR QTMLQALREV EDARANLVSS DEATRRLGSA LAASDKALGL ANQLYKGG
Validated NT Sequence
gcgccgccct tatagagctg attcgccagc ccgagcgctt tatcggaagc cgccaatgcg ctacccagcc ttcgcgtcgc ctcatcggaa ctgacgagat tcgcacgcgc atcctcgact tcccgcagtg cttgcagcat cgtctgccgt aacccgagtt ccgactcgcg catccggctt tcgctctgcg agatatcggc ggtaatccgc cccgcgttga agatcgggct cgtcgcgctc agtgccgcgc tgaacaggtt atcggtcaac gtcggcagcc cgagatagga agccgccaga atcccgtccg tcaggttcaa cgaaaacttc ggataccgct cggcctttgc cacgcccact tgcgctgccc gtcgctcgac ctgcgcatag gcagtcagca catccggccg ccgcaacaac gcctgcgaag gcagcgtccc cggcgcgcca atcggcggcg cgggaatctc cccggcctgc gccagcacga gccgatcgac cgattccggc gtccgccccg aatacacggc gatcagattg agctgatgcg aaatctgcgc ctgcgtcggc ggaatccgcg cctcgagcgc gtcnagctgg ttctgcgcac gcgtcacgtc nagttccgtc gacagcccga acgccngccg ctngcgtgtg anttcgagcg catgctgncg gancttcgcg ttgtcctgcn nnatcttcag ttnctgctgc gcccagcgca gatcgacata cgcggannnc gcatcgncgg caagcgcang ncgcatcgcn tnntccgngt gntttnnccn gaccaactnc gcnngagcgg ccancnnann gannnnntca ccncngaaca catcgngcga ccagctcagc gcgagcccgg ccccagcctn ccgcacataa ccgagcggng gcggcgtgtt ntgccgcgcg tcggccgcat gcgcggtcgc gtcgagttcg ggcagcagca ccgcgcgttt ntgcacggtc agcgcctgcg cctgcttcac gcgttcggcg gcggcttgca gatcgaggtt cccgtcgagc acggtcnnga tcagccgg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPAQHPALN ESVQTLAPTV WDTDVPRADV DAAAWWAQFH DPVLDRLIAT VLDGNLDLQA AAERVKQAQA LTVQKRAVLL PELDATAHAA DARQNTPPPL GYVRQAGAGL ALSWSPDVFG GERLDLLAAQ AELVGQKHAE DAMRLALAAD AASAYVDLRW AQQELKILQD NAKIRQHALE LTRKRQAFGL STELDVTRAQ NQLDALEARI PPTQAQISHQ LNLIAVYSGR TPESVDRLVL AQAGEIPAPP IGAPGTLPSQ ALLRRPDVLT AYAQVERRAA QVGVAKAERY PKFSLNLTDG ILAASYLGLP TLTDNLFSAA LSATSPIFNA GRITADISQS ESRMRESELG LRQTMLQALR EVEDARANLV SSDEATRRLG SALAASDKAL GLANQLYKGG ATDFLDVLSA QEVYLRDSDS LNQVKREHAL AAVALYRSLG GGWSRNEAAG GPRAETEAVA KN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcccgcgcag catccggcgc tgaacgaatc cgttcagacg ctcgcgccca ccgtgtggga taccgacgtg ccgcgcgcgg acgtcgatgc ggccgcctgg tgggcgcagt tccacgaccc cgtgctcgac cggctgatcg cgaccgtgct cgacgggaac ctcgatctgc aagccgccgc cgaacgcgtg aagcaggcgc aggcgctgac cgtgcagaaa cgcgcggtgc tgctgcccga actcgacgcg accgcgcatg cggccgacgc gcggcagaac acgccgccgc cgctcggtta tgtgcggcag gctggggccg ggctcgcgct gagctggtcg cccgatgtgt tcggcggtga acggctcgat ttgctggccg ctcaagcgga gttggtcggg caaaagcacg cggaagatgc gatgcgcctt gcgcttgccg ccgatgcggc atccgcgtat gtcgatctgc gctgggcgca gcaggaactg aagatcctgc aggacaacgc gaagatccgg cagcatgcgc tcgaactcac acgcaagcgg caggcgttcg ggctgtcgac ggaactcgac gtgacgcgtg cgcagaacca gctcgacgcg ctcgaggcgc ggattccgcc gacgcaggcg cagatttcgc atcagctcaa tctgatcgcc gtgtattcgg ggcggacgcc ggaatcggtc gatcggctcg tgctggcgca ggccggggag attcccgcgc cgccgattgg cgcgccgggg acgctgcctt cgcaggcgtt gttgcggcgg ccggatgtgc tgactgccta tgcgcaggtc gagcgacggg cagcgcaagt gggcgtggca aaggccgagc ggtatccgaa gttttcgttg aacctgacgg acgggattct ggcggcttcc tatctcgggc tgccgacgtt gaccgataac ctgttcagcg cggcactgag cgcgacgagc ccgatcttca acgcggggcg gattaccgcc gatatctcgc agagcgaaag ccggatgcgc gagtcggaac tcgggttacg gcagacgatg ctgcaagcac tgcgggaagt cgaggatgcg cgtgcgaatc tcgtcagttc cgatgaggcg acgcgaaggc tgggtagcgc attggcggct tccgataaag cgctcgggct ggcgaatcag ctctataagg gcggcgcgac ggattttctg gatgtgctgt cggcgcagga ggtttatctg cgggattcgg attcgctcaa tcaggtcaag cgcgaacatg cgctcgcggc ggtggcgttg tatcggtcac ttgggggtgg gtggagcagg aatgaggcgg cgggtgggcc gcgggcggaa accgaagctg tggcaaagaa ctaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa