SSGCID: Target Page

BuceA.01673.k

Outer membrane efflux protein

CENTER ID:	BuceA.01673.k
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01673.k.A2.GE31841	Mature protein( BuceA.01673.k )		27	459

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01673.k.A2.PW31754	Mature protein( BuceA.01673.k )		27	459

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.5967
RefSeq:	YP_002234788.1
UniProt:	B4EFY5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTITHSSPLR HLGAFACAAA LLAGCAGTRH DPLPPVAMPA NWAAPVTADA PPAARDWWRS FGDPQLDALI DDALRANNDL AIAAIRVYRA QLQAGLVDTN LTPNVTLGAN GAVSRTLDTH RTSQSSGLNG SLSYEIDLWG RLAALRDAAH WEADATAADL EAARLSLIGT TAALYWQIGY LNRQIALGDA NIAYAVRTLA LVRSRHAAGA VSGLDLAQAE QSLAAQRAAQ TQLIQQRTEN RHALAILFDR PPQQLAAEPA ALPDAAPPDV AAGLPASLLG RRPDLRAAEF RLRESLAQVD ATRTSFYPTF TLTGSAGTTS TSLERVLTNP VGTLGLGLAL PFIQWNTMQL QIKVSKSQYE EAVVGFRQRL YTALAEVENA LSARVQLERE AEQRALSLAQ AQRAERLAAA RFAAGATAVQ PWLDQQQVLR SAQSADELTR LNRLNNQMKL YRALGGGTS

NT Sequence

ATGACCATCA CGCATTCCTC CCCGCTTCGC CACCTCGGCG CATTCGCCTG CGCGGCCGCT TTGCTCGCCG GCTGCGCGGG TACGCGCCAC GATCCGCTGC CGCCCGTCGC GATGCCCGCG AACTGGGCCG CGCCGGTCAC GGCCGACGCG CCGCCCGCCG CGCGCGACTG GTGGCGCAGC TTCGGCGATC CGCAACTCGA CGCGCTGATC GACGACGCGC TGCGCGCCAA CAACGACCTC GCGATCGCGG CGATCCGCGT GTATCGCGCG CAGTTGCAGG CCGGGCTCGT CGATACGAAC CTGACGCCCA ACGTGACGCT CGGCGCGAAC GGCGCCGTGT CGCGCACGCT CGATACGCAC CGGACGAGCC AGTCGAGCGG CCTCAACGGT TCGCTCAGCT ACGAAATCGA CCTGTGGGGC CGGCTGGCCG CGCTGCGCGA TGCCGCGCAC TGGGAAGCCG ACGCGACGGC CGCCGATCTC GAAGCCGCGC GGCTGTCGCT GATCGGCACG ACCGCGGCGC TGTACTGGCA GATCGGCTAT CTGAACCGGC AGATCGCGCT CGGCGACGCG AACATCGCGT ATGCGGTGCG CACGCTCGCG CTCGTCCGGT CGCGGCACGC GGCCGGCGCC GTGTCGGGGC TCGATCTCGC GCAGGCCGAA CAGAGCCTCG CCGCGCAACG CGCGGCGCAG ACGCAACTGA TCCAGCAGCG CACCGAGAAC CGCCACGCGC TCGCGATCCT GTTCGACCGG CCGCCGCAGC AACTGGCGGC CGAGCCGGCG GCACTGCCGG ATGCCGCACC GCCCGACGTG GCGGCCGGCC TGCCCGCGAG CCTGCTCGGC CGGCGCCCCG ATCTGCGGGC GGCGGAATTC CGGCTGCGCG AATCGCTCGC GCAGGTCGAC GCGACGCGCA CGAGCTTCTA CCCGACCTTC ACGCTGACCG GCAGCGCCGG CACGACGAGC ACGAGCCTCG AGCGCGTGCT GACGAACCCG GTCGGCACGC TCGGGCTCGG CCTCGCGCTG CCGTTCATCC AGTGGAACAC GATGCAGTTG CAGATCAAGG TGTCGAAGTC GCAGTACGAG GAAGCGGTCG TCGGGTTCCG CCAGCGGCTC TACACGGCGC TCGCGGAAGT CGAGAATGCG CTGTCGGCGC GCGTGCAGCT CGAACGCGAG GCCGAACAGC GCGCGCTGTC GCTCGCGCAG GCGCAGCGTG CCGAACGGCT GGCCGCCGCG CGCTTCGCGG CCGGCGCGAC CGCCGTGCAG CCGTGGCTCG ACCAGCAACA GGTGCTGAGG AGCGCGCAGA GCGCCGACGA GCTGACCCGC CTGAACCGGC TCAACAACCA GATGAAGCTC TACCGCGCGC TCGGCGGCGG GACGTCC

Details for BuceA.01673.k.A2.GE31841

HARVESTED ON:	3/22/2011
SEQUENCED ON:	3/25/2011
EXPECTED MW:	48kDa
OBSERVED MW:	48kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	52
PERCENT COVERAGE:	36

Validated AA Sequence

MFXXTPXXXX XXXXXXXXXX SXPGSWFDGY APRSAAARRX XANWAXXVTX XXPXARDWWR SFXXPQLDAL IDDAXXANXD LAIAAIRVYR XQLQXGXXXT NLTPNXTLGA NGAVXRTLDX HRTXQSSGLN GSXXXXIXXG AGWPRCXXXR TGKPTRXXXX XXPRGXX

Validated NT Sequence

gcnnnncagc catgtttann nnnactcctn tnnnnnannn annnnnnann annannnnnn nncanngnng ctcanacccn gggtcctggt tcgatgggta cgcgccacga tccgctgccg cccgtcgcnn nnncgcgaac tgggccgngn cggtcacggn cganncgccn nccgcgcgcg actggtggcg cagcttcngc natccgcaac tcgacgcgct gatcgacgac gcgntgcncg ccaacnacga cctcgcgatc gcggcgatcc gcgtgtatcg cgngcagttg caggncgggn tcntnnanac gaacctgacg cccaacntga cgctcggngc gaacggngcc gtgtnncgca cgctcgatnc ncaccggacg agncagtcga gcggcctcaa cggttcgntc anntncnaaa tcnactgngg ggccggctgg ccgcgctgcn ngnngncncg cactgggaag ccgacgcgac ngncgncnan nnnnnnnccg cgcggctgnn nntgatcnnc acgaccncng cgctgtncng nnngannngn nnnctnaacc ggcngatnnn nnncnnnnac gnnnanntnn nnnnngnggt g

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMGTRHDPLP PVAMPANWAA PVTADAPPAA RDWWRSFGDP QLDALIDDAL RANNDLAIAA IRVYRAQLQA GLVDTNLTPN VTLGANGAVS RTLDTHRTSQ SSGLNGSLSY EIDLWGRLAA LRDAAHWEAD ATAADLEAAR LSLIGTTAAL YWQIGYLNRQ IALGDANIAY AVRTLALVRS RHAAGAVSGL DLAQAEQSLA AQRAAQTQLI QQRTENRHAL AILFDRPPQQ LAAEPAALPD AAPPDVAAGL PASLLGRRPD LRAAEFRLRE SLAQVDATRT SFYPTFTLTG SAGTTSTSLE RVLTNPVGTL GLGLALPFIQ WNTMQLQIKV SKSQYEEAVV GFRQRLYTAL AEVENALSAR VQLEREAEQR ALSLAQAQRA ERLAAARFAA GATAVQPWLD QQQVLRSAQS ADELTRLNRL NNQMKLYRAL GGGTS

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggtacgcgc cacgatccgc tgccgcccgt cgcgatgccc gcgaactggg ccgcgccggt cacggccgac gcgccgcccg ccgcgcgcga ctggtggcgc agcttcggcg atccgcaact cgacgcgctg atcgacgacg cgctgcgcgc caacaacgac ctcgcgatcg cggcgatccg cgtgtatcgc gcgcagttgc aggccgggct cgtcgatacg aacctgacgc ccaacgtgac gctcggcgcg aacggcgccg tgtcgcgcac gctcgatacg caccggacga gccagtcgag cggcctcaac ggttcgctca gctacgaaat cgacctgtgg ggccggctgg ccgcgctgcg cgatgccgcg cactgggaag ccgacgcgac ggccgccgat ctcgaagccg cgcggctgtc gctgatcggc acgaccgcgg cgctgtactg gcagatcggc tatctgaacc ggcagatcgc gctcggcgac gcgaacatcg cgtatgcggt gcgcacgctc gcgctcgtcc ggtcgcggca cgcggccggc gccgtgtcgg ggctcgatct cgcgcaggcc gaacagagcc tcgccgcgca acgcgcggcg cagacgcaac tgatccagca gcgcaccgag aaccgccacg cgctcgcgat cctgttcgac cggccgccgc agcaactggc ggccgagccg gcggcactgc cggatgccgc accgcccgac gtggcggccg gcctgcccgc gagcctgctc ggccggcgcc ccgatctgcg ggcggcggaa ttccggctgc gcgaatcgct cgcgcaggtc gacgcgacgc gcacgagctt ctacccgacc ttcacgctga ccggcagcgc cggcacgacg agcacgagcc tcgagcgcgt gctgacgaac ccggtcggca cgctcgggct cggcctcgcg ctgccgttca tccagtggaa cacgatgcag ttgcagatca aggtgtcgaa gtcgcagtac gaggaagcgg tcgtcgggtt ccgccagcgg ctctacacgg cgctcgcgga agtcgagaat gcgctgtcgg cgcgcgtgca gctcgaacgc gaggccgaac agcgcgcgct gtcgctcgcg caggcgcagc gtgccgaacg gctggccgcc gcgcgcttcg cggccggcgc gaccgccgtg cagccgtggc tcgaccagca acaggtgctg aggagcgcgc agagcgccga cgagctgacc cgcctgaacc ggctcaacaa ccagatgaag ctctaccgcg cgctcggcgg cgggacgtcc taaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa

Details for BuceA.01673.k.A2.PW31754

PURIFICATION DATe:	5/27/2011
CONCENTRATION:	24.35mg/ml
OBSERVED MW:	data unavailable
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	11
VIAL VOLUME:	100µl
PERCENT IDENTITY:	52
PERCENT COVERAGE:	36

Protocol Notes

notes unavailable

Validated AA Sequence

MFXXTPXXXX XXXXXXXXXX SXPGSWFDGY APRSAAARRX XANWAXXVTX XXPXARDWWR SFXXPQLDAL IDDAXXANXD LAIAAIRVYR XQLQXGXXXT NLTPNXTLGA NGAVXRTLDX HRTXQSSGLN GSXXXXIXXG AGWPRCXXXR TGKPTRXXXX XXPRGXX

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGTACGCGC CACGATCCGC TGCCGCCCGT CGCGATGCCC GCGAACTGGG CCGCGCCGGT CACGGCCGAC GCGCCGCCCG CCGCGCGCGA CTGGTGGCGC AGCTTCGGCG ATCCGCAACT CGACGCGCTG ATCGACGACG CGCTGCGCGC CAACAACGAC CTCGCGATCG CGGCGATCCG CGTGTATCGC GCGCAGTTGC AGGCCGGGCT CGTCGATACG AACCTGACGC CCAACGTGAC GCTCGGCGCG AACGGCGCCG TGTCGCGCAC GCTCGATACG CACCGGACGA GCCAGTCGAG CGGCCTCAAC GGTTCGCTCA GCTACGAAAT CGACCTGTGG GGCCGGCTGG CCGCGCTGCG CGATGCCGCG CACTGGGAAG CCGACGCGAC GGCCGCCGAT CTCGAAGCCG CGCGGCTGTC GCTGATCGGC ACGACCGCGG CGCTGTACTG GCAGATCGGC TATCTGAACC GGCAGATCGC GCTCGGCGAC GCGAACATCG CGTATGCGGT GCGCACGCTC GCGCTCGTCC GGTCGCGGCA CGCGGCCGGC GCCGTGTCGG GGCTCGATCT CGCGCAGGCC GAACAGAGCC TCGCCGCGCA ACGCGCGGCG CAGACGCAAC TGATCCAGCA GCGCACCGAG AACCGCCACG CGCTCGCGAT CCTGTTCGAC CGGCCGCCGC AGCAACTGGC GGCCGAGCCG GCGGCACTGC CGGATGCCGC ACCGCCCGAC GTGGCGGCCG GCCTGCCCGC GAGCCTGCTC GGCCGGCGCC CCGATCTGCG GGCGGCGGAA TTCCGGCTGC GCGAATCGCT CGCGCAGGTC GACGCGACGC GCACGAGCTT CTACCCGACC TTCACGCTGA CCGGCAGCGC CGGCACGACG AGCACGAGCC TCGAGCGCGT GCTGACGAAC CCGGTCGGCA CGCTCGGGCT CGGCCTCGCG CTGCCGTTCA TCCAGTGGAA CACGATGCAG TTGCAGATCA AGGTGTCGAA GTCGCAGTAC GAGGAAGCGG TCGTCGGGTT CCGCCAGCGG CTCTACACGG CGCTCGCGGA AGTCGAGAAT GCGCTGTCGG CGCGCGTGCA GCTCGAACGC GAGGCCGAAC AGCGCGCGCT GTCGCTCGCG CAGGCGCAGC GTGCCGAACG GCTGGCCGCC GCGCGCTTCG CGGCCGGCGC GACCGCCGTG CAGCCGTGGC TCGACCAGCA ACAGGTGCTG AGGAGCGCGC AGAGCGCCGA CGAGCTGACC CGCCTGAACC GGCTCAACAA CCAGATGAAG CTCTACCGCG CGCTCGGCGG CGGGACGTCC TAAACAGCAC GAACAAGTTC TGCAGCCAAG CTTCTCGAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAA