BuceA.01673.h

Putative fusaric acid resistance outer membrane efflux protein

CENTER ID: BuceA.01673.h
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01673.h.A2.GE31838 Mature protein( BuceA.01673.h ) 21 486
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1225
RefSeq: YP_002230317.1
UniProt: B4EDQ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTVARFFGLI AILYFAVLSG CADMRGIGPL SNLQDADELD AGSAIRTARA PSPLPTGGWW RAYGDTQLNR LIDTALAHNP SLSAAHDRVR VAQAMARAAH ANELPKVDGS LRLLRQHWPD DAYYGPGALG NSNTWNNTGG MNLAWHIDLW GRDKNIASSA RDRFRAREAD ELAARLQLQT NIVYAYIDLA LNIALLANEQ ANTELQQKTV ELVRKRLKAG IATQLELSEA ESPLPGYVKR ITAWKAAVEL NQHQLAALIG EGPGAGTTIQ RPQIVFEKPI GLPSTLPSEL VGRRPDVIAA RWEVESRARG VDVARAAFYP NIDLLASIGS FGAAAGFIDF LRAANGGWSA GPALTLPIFE GGRLRARLAA ATAEYDEAVD RYNRAIIEAI KDIADNVVRV RSLATQMDAD RRSISVAARS LDLARISQRR GLVNYKNVLV AQANLLQAQN SAIRTEVDLL KAHAALMASL GGSDPTPRRA NTDASR
NT Sequence
ATGACCGTGG CCCGATTTTT CGGCTTAATC GCTATCTTAT ACTTCGCAGT ACTTAGTGGA TGCGCTGACA TGCGTGGAAT CGGACCATTA TCGAATCTGC AGGACGCCGA CGAGCTGGAC GCGGGAAGCG CGATCCGCAC GGCTCGCGCC CCCTCGCCCC TACCCACCGG CGGGTGGTGG CGGGCTTATG GTGACACTCA ACTCAATCGG CTGATCGATA CGGCACTTGC GCACAATCCC TCGCTTTCAG CTGCCCACGA CCGCGTGCGT GTCGCACAGG CCATGGCTCG CGCAGCGCAC GCCAACGAAC TGCCGAAGGT GGACGGATCT CTCAGGCTAT TGCGGCAGCA TTGGCCCGAC GATGCCTACT ACGGACCTGG CGCGCTCGGC AACAGCAATA CTTGGAACAA CACGGGTGGG ATGAACCTCG CCTGGCACAT CGACCTATGG GGTCGCGACA AAAACATCGC TTCAAGCGCA CGCGACCGTT TTCGCGCGAG AGAAGCGGAC GAGCTGGCGG CACGGCTTCA GTTGCAGACG AACATAGTCT ATGCCTATAT CGATCTGGCT CTTAATATCG CTTTGCTCGC CAATGAACAG GCAAATACTG AACTCCAGCA GAAGACCGTG GAGCTGGTTC GCAAGCGTCT GAAGGCCGGT ATCGCCACGC AACTCGAACT GAGCGAAGCC GAGTCGCCAC TTCCCGGCTA CGTCAAACGG ATCACGGCCT GGAAAGCTGC CGTCGAACTG AACCAACATC AGCTCGCCGC GCTCATCGGC GAAGGGCCCG GCGCAGGGAC GACGATCCAG CGTCCGCAGA TCGTGTTTGA GAAGCCAATT GGGCTGCCTT CGACGCTGCC GAGCGAACTG GTCGGCCGCC GGCCCGACGT CATTGCCGCT CGCTGGGAAG TCGAGTCGAG AGCGCGAGGT GTTGATGTCG CGAGGGCGGC GTTCTATCCA AACATCGACC TGCTGGCTTC AATCGGTAGC TTTGGCGCAG CCGCCGGTTT CATCGATTTC CTGCGCGCCG CGAACGGCGG ATGGTCGGCC GGACCGGCGC TGACACTGCC GATCTTCGAG GGCGGCCGCC TGCGTGCGCG GCTCGCTGCC GCGACTGCGG AATACGACGA AGCCGTTGAC CGGTATAACC GGGCAATTAT CGAGGCGATC AAGGACATCG CCGACAACGT CGTTCGCGTG CGCTCGCTCG CAACGCAGAT GGACGCCGAC AGAAGGTCCA TCTCCGTCGC CGCGCGTTCG TTGGATCTGG CGCGCATCAG TCAGCGCCGT GGACTGGTCA ACTACAAGAA TGTTTTGGTC GCCCAGGCCA ATCTGCTCCA GGCACAGAAC AGTGCGATCC GGACCGAAGT CGACCTGCTG AAAGCTCACG CCGCTTTGAT GGCCTCGCTC GGAGGCAGCG ACCCGACGCC TCGACGTGCA AACACCGACG CTAGTCGT
Details for BuceA.01673.h.A2.GE31838
HARVESTED ON: 3/22/2011
SEQUENCED ON: 3/25/2011
EXPECTED MW: 52kDa
OBSERVED MW: 52kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 20
PERCENT COVERAGE: 40
Validated AA Sequence
MFXXTXXXXX XXXXXXXXXX XXXLXXXAXX XXXXDHYRIC XTPTSWTREA RSXXLAPPRP XXPXGGGXXM VXXTQSADXY GTCAXSLAXX CPRPRACRTG HGSRSARQRT AEGGRISQAI AAALARRCLL RTWRARQQQY LEQHGWDEPR LAHRPMGSRQ KHRFKRTRPF SRERSGRAGG TASVADEHXL CLYRSGS
Validated NT Sequence
gcnnnncagc catgtttann nnnactcnnn nnannnanan nnnnnnacan nnnnnnnnnn nnannnnnnn nnnncnggnc ctggnnnnnn gngcncngnn nngcnngnnn ncngaccatt atcgaatctg cnngacgccn acgagctgga cgcgggaagc gcgatcnnnn nggctcgcgc cccctcgccc ctncncaccn nnnggtggtg gcngacnnat ggtgcnanna actcaatcgg ctgatcnata cggcacttgc gcacnatccc tcgctttnnn ntgcccacga ccgcgtgcgt gtcgcacagg ccatggctcg cgcagcgcac gccaacgaac tgccgaaggt ggacggatct ctcaggctat tgcggcagca ttggcccgac gatgcctact acggacctgg cgcgctcggc aacagcaata cttggaacaa cacgggtggg atgaacctcg cctggcacat cgacctatgg ggtcgcgaca aaaacatcgc ttcaagcgca cgcgaccgtt ttcgcgcgag agaagcggac gagctggcgg cacggcttcn gttgcagacg aacatagnct atgcctatat cgatctggct cttaatatcg cttttgctcg ccaatgnnca ggcaaatact gaactccann nnnnnanngn ggagntgnnn cgcaagcntc nganggccgg tatcnnnncg cnanctcgaa ctgagcgaan ncnagtcnnc acntcccggc tacntcaaan ggancacggc cctnganagc tgccgncnaa ctgaanccaa cnncnnntcg nnncgnnnan ncgnncnann nggcccggcg cagggnnnnn nnnnnnnntc cgcaaatnnn nnnnnaaaaa nccaattggn ctggccttcc na
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMCADMRGIG PLSNLQDADE LDAGSAIRTA RAPSPLPTGG WWRAYGDTQL NRLIDTALAH NPSLSAAHDR VRVAQAMARA AHANELPKVD GSLRLLRQHW PDDAYYGPGA LGNSNTWNNT GGMNLAWHID LWGRDKNIAS SARDRFRARE ADELAARLQL QTNIVYAYID LALNIALLAN EQANTELQQK TVELVRKRLK AGIATQLELS EAESPLPGYV KRITAWKAAV ELNQHQLAAL IGEGPGAGTT IQRPQIVFEK PIGLPSTLPS ELVGRRPDVI AARWEVESRA RGVDVARAAF YPNIDLLASI GSFGAAAGFI DFLRAANGGW SAGPALTLPI FEGGRLRARL AAATAEYDEA VDRYNRAIIE AIKDIADNVV RVRSLATQMD ADRRSISVAA RSLDLARISQ RRGLVNYKNV LVAQANLLQA QNSAIRTEVD LLKAHAALMA SLGGSDPTPR RANTDASR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtgcgctgac atgcgtggaa tcggaccatt atcgaatctg caggacgccg acgagctgga cgcgggaagc gcgatccgca cggctcgcgc cccctcgccc ctacccaccg gcgggtggtg gcgggcttat ggtgacactc aactcaatcg gctgatcgat acggcacttg cgcacaatcc ctcgctttca gctgcccacg accgcgtgcg tgtcgcacag gccatggctc gcgcagcgca cgccaacgaa ctgccgaagg tggacggatc tctcaggcta ttgcggcagc attggcccga cgatgcctac tacggacctg gcgcgctcgg caacagcaat acttggaaca acacgggtgg gatgaacctc gcctggcaca tcgacctatg gggtcgcgac aaaaacatcg cttcaagcgc acgcgaccgt tttcgcgcga gagaagcgga cgagctggcg gcacggcttc agttgcagac gaacatagtc tatgcctata tcgatctggc tcttaatatc gctttgctcg ccaatgaaca ggcaaatact gaactccagc agaagaccgt ggagctggtt cgcaagcgtc tgaaggccgg tatcgccacg caactcgaac tgagcgaagc cgagtcgcca cttcccggct acgtcaaacg gatcacggcc tggaaagctg ccgtcgaact gaaccaacat cagctcgccg cgctcatcgg cgaagggccc ggcgcaggga cgacgatcca gcgtccgcag atcgtgtttg agaagccaat tgggctgcct tcgacgctgc cgagcgaact ggtcggccgc cggcccgacg tcattgccgc tcgctgggaa gtcgagtcga gagcgcgagg tgttgatgtc gcgagggcgg cgttctatcc aaacatcgac ctgctggctt caatcggtag ctttggcgca gccgccggtt tcatcgattt cctgcgcgcc gcgaacggcg gatggtcggc cggaccggcg ctgacactgc cgatcttcga gggcggccgc ctgcgtgcgc ggctcgctgc cgcgactgcg gaatacgacg aagccgttga ccggtataac cgggcaatta tcgaggcgat caaggacatc gccgacaacg tcgttcgcgt gcgctcgctc gcaacgcaga tggacgccga cagaaggtcc atctccgtcg ccgcgcgttc gttggatctg gcgcgcatca gtcagcgccg tggactggtc aactacaaga atgttttggt cgcccaggcc aatctgctcc aggcacagaa cagtgcgatc cggaccgaag tcgacctgct gaaagctcac gccgctttga tggcctcgct cggaggcagc gacccgacgc ctcgacgtgc aaacaccgac gctagtcgtt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa