SSGCID: Target Page

BuceA.01673.g

Efflux system outer membrane protein

CENTER ID:	BuceA.01673.g
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01673.g.A2.GE34650	Mature protein( BuceA.01673.g )		18	463

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2221
RefSeq:	YP_002231263.1
UniProt:	B4ED58

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLGSAALLAG CSLAPTYRAP DVPVPAAFRE TGPWIDAAPA DRLPRDGWWR VYGDAALDRL EPLVARANPD LAAAVARHDE AAALFDEAHA ALLPTVGIGA ETDRDRQSAR RPLRGSGQPD VYESNTLAAG IGYDVDLWGK VRNTVAAGRD DAQAAADDLA SVQLSLQAGV ASTYFDLAGL DREADTLTQT IDTYRRAWQL TVSRHRGGLA SGLDVARAQT QLALAQARAS DIAARRALDE HAIAALVGAS ASTFTLPSAA ALPGVPPIPT GLASTLLERR PDVAAAERRV AAANARIGVA RAAFFPDLSL GLDAGFQSDT FSPWLAAPNE IWSVGPRLAM TLFDGGLRAA GVRHARAQFA EQGARYRSVV LLAFREVEDD LALLHRLGDE ADQDDAALVA ARQSLALAMS RYRDGAVSYL DVVTAQTTEL DTQIAALDVD ARRLQASVGL VRALGGGWRG ARS

NT Sequence

TTGCTGGGCA GCGCCGCACT GCTGGCCGGC TGTTCGCTGG CGCCGACGTA TCGCGCGCCC GACGTTCCGG TGCCGGCCGC GTTTCGTGAA ACGGGCCCGT GGATCGACGC CGCGCCGGCC GACCGCCTGC CGCGCGACGG CTGGTGGCGC GTGTACGGCG ACGCGGCGCT CGACCGGCTC GAACCGCTCG TCGCGCGCGC GAATCCCGAT CTCGCCGCCG CCGTCGCGCG GCACGACGAA GCCGCCGCGC TGTTCGACGA GGCCCATGCG GCGCTGCTGC CGACCGTGGG CATCGGGGCC GAGACCGACC GCGACCGGCA GTCGGCGCGC CGGCCGTTGC GCGGCAGCGG TCAGCCTGAC GTCTACGAAT CGAACACGCT CGCGGCCGGC ATCGGCTACG ACGTCGATCT GTGGGGCAAG GTCCGCAATA CGGTCGCGGC CGGCCGCGAC GACGCGCAGG CGGCCGCCGA CGATCTCGCG TCGGTCCAGC TGAGCCTGCA GGCCGGCGTC GCGAGTACCT ACTTCGATCT CGCGGGTCTC GACCGCGAAG CCGACACGCT CACGCAAACC ATCGACACGT ACCGCCGCGC GTGGCAACTG ACCGTCAGCC GCCATCGCGG CGGCCTCGCT TCGGGGCTCG ACGTGGCGCG TGCGCAGACG CAACTCGCGC TCGCCCAGGC CCGCGCGTCC GACATCGCCG CCCGCCGCGC GCTCGACGAG CACGCGATCG CGGCGCTCGT CGGGGCGTCG GCATCGACGT TCACGCTGCC GTCCGCCGCG GCACTGCCCG GCGTGCCACC GATCCCGACG GGACTTGCCT CGACGCTGCT CGAACGCCGG CCGGACGTCG CCGCCGCCGA GCGCCGCGTC GCGGCCGCGA ACGCGCGAAT CGGCGTCGCG CGCGCGGCTT TTTTCCCGGA CCTGTCGCTC GGCCTCGACG CGGGCTTCCA GAGCGATACG TTCTCGCCGT GGCTCGCCGC GCCGAACGAG ATCTGGTCGG TCGGGCCGCG TCTCGCGATG ACGCTGTTCG ACGGCGGCCT GCGCGCGGCC GGCGTGCGCC ACGCCCGTGC GCAGTTCGCC GAACAGGGGG CGCGCTACCG GTCCGTCGTG CTGCTGGCGT TCCGCGAAGT CGAGGACGAT CTCGCGCTGC TGCACCGCCT CGGCGACGAA GCCGACCAGG ACGACGCGGC GCTCGTCGCG GCGCGGCAGT CGCTGGCGCT CGCGATGTCG CGCTATCGCG ACGGCGCGGT CAGCTATCTC GACGTGGTCA CCGCGCAGAC GACCGAACTC GATACGCAGA TCGCGGCGCT CGATGTCGAC GCGCGGCGCC TGCAGGCGTC CGTCGGGCTC GTACGCGCGC TCGGCGGCGG CTGGCGCGGC GCGCGTTCA

Details for BuceA.01673.g.A2.GE34650

HARVESTED ON:	1/31/2012
SEQUENCED ON:	3/16/2012
EXPECTED MW:	49kDa
OBSERVED MW:	47kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	92
PERCENT COVERAGE:	73

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMRAPDVPVP AAFRETGPWI DAAPADRLPR DGWWRVYGDA ALDRLEPLVA RANPDLAAAV ARHDEAAALF DEAHAALLPT VGIGAETDRD RQSARRPLRG SGQPDVYESN TLAAGIGYDV DLWGKVRNTV AAGRDDAQAA ADDLASVQLS LQAGVASTYF DLAGLDREAD TLTQTIDTYR RAWQLTVSRH RGGLASGLDV ARAQTQLALA QARASDIAAR RALDEHAIAA LVGASASTFT LPSAAALPGV PPIPTGLAST LLERRPDVAA AERRVAAANA RIGVARAAFF XXXSLXXDXA XXXIXXXXGX XXXXIWXVXX VXX

Validated NT Sequence

ttgtttactt taagaaggag atataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atgcgcgcgc ccgacgttcc ggtgccggcc gcgtttcgtg aaacgggccc gtggatcgac gccgcgccgg ccgaccgcct gccgcgcgac ggctggtggc gcgtgtacgg cgacgcggcg ctcgaccggc tcgaaccgct cgtcgcgcgc gcgaatcccg atctcgccgc cgccgtcgcg cggcacgacg aagccgccgc gctgttcgac gaggcccatg cggcgctgct gccgaccgtg ggcatcgggg ccgagaccga ccgcgaccgg cagtcggcgc gccggccgtt gcgcggcagc ggtcagcctg acgtctacga atcgaacacg ctcgcggccg gcatcggcta cgacgtcgat ctgtggggca aggtccgcaa tacggtcgcg gccggccgcg acgacgcgca ggcggccgcc gacgatctcg cgtcggtcca gctgagcctg caggccggcg tcgcgagtac ctacttcgat ctcgcgggtc tcgaccgcga agccgacacg ctcacgcaaa ccatcgacac gtaccgccgc gcgtggcaac tgaccgtcag ccgccatcgc ggcggcctcg cttcggggct cgacgtggcg cgtgcgcaga cgcaactcgc gctcgcccag gcccgcgcgt ccgacatcgc cgcccgccgc gcgctcgacg agcacgcgat cgcggcgctc gtcggggcgt cggcatcgac gttcacgctg ccgtccgccg cggcactgcc cggcgtgcca ccgatcccga cgggacttgc ctcgacgctg ctcgaacgcc ggccggacgt cgccgccgcc gagcgccgcg tcgcggcngc gaacgcgcga atcggcgtcg cgcgcgcngc ttttttccnn nacntgtcgc tnngnnnnga cncggcttnc ngancgatac ntnnnnnnnn nggcncncnc nnnaannaga tctggncngt cngnnnngtc tnnnnatgac nctnnnnang nnnngnnnnn nnnnnnnnnn nnccnnnnnn annnncnann ggggnnnnnc tncnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnannnnnn nnnnnnnnnc nnnnnnnnnn nnnnnnnann nnnnng

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcgcgccc gacgttccgg tgccggccgc gtttcgtgaa acgggcccgt ggatcgacgc cgcgccggcc gaccgcctgc cgcgcgacgg ctggtggcgc gtgtacggcg acgcggcgct cgaccggctc gaaccgctcg tcgcgcgcgc gaatcccgat ctcgccgccg ccgtcgcgcg gcacgacgaa gccgccgcgc tgttcgacga ggcccatgcg gcgctgctgc cgaccgtggg catcggggcc gagaccgacc gcgaccggca gtcggcgcgc cggccgttgc gcggcagcgg tcagcctgac gtctacgaat cgaacacgct cgcggccggc atcggctacg acgtcgatct gtggggcaag gtccgcaata cggtcgcggc cggccgcgac gacgcgcagg cggccgccga cgatctcgcg tcggtccagc tgagcctgca ggccggcgtc gcgagtacct acttcgatct cgcgggtctc gaccgcgaag ccgacacgct cacgcaaacc atcgacacgt accgccgcgc gtggcaactg accgtcagcc gccatcgcgg cggcctcgct tcggggctcg acgtggcgcg tgcgcagacg caactcgcgc tcgcccaggc ccgcgcgtcc gacatcgccg cccgccgcgc gctcgacgag cacgcgatcg cggcgctcgt cggggcgtcg gcatcgacgt tcacgctgcc gtccgccgcg gcactgcccg gcgtgccacc gatcccgacg ggacttgcct cgacgctgct cgaacgccgg ccggacgtcg ccgccgccga gcgccgcgtc gcggccgcga acgcgcgaat cggcgtcgcg cgcgcggctt ttttcccgga cctgtcgctc ggcctcgacg cgggcttcca gagcgatacg ttctcgccgt ggctcgccgc gccgaacgag atctggtcgg tcgggccgcg tctcgcgatg acgctgttcg acggcggcct gcgcgcggcc ggcgtgcgcc acgcccgtgc gcagttcgcc gaacaggggg cgcgctaccg gtccgtcgtg ctgctggcgt tccgcgaagt cgaggacgat ctcgcgctgc tgcaccgcct cggcgacgaa gccgaccagg acgacgcggc gctcgtcgcg gcgcggcagt cgctggcgct cgcgatgtcg cgctatcgcg acggcgcggt cagctatctc gacgtggtca ccgcgcagac gaccgaactc gatacgcaga tcgcggcgct cgatgtcgac gcgcggcgcc tgcaggcgtc cgtcgggctc gtacgcgcgc tcggcggcgg ctggcgcggc gcgcgttcat aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa