BuceA.01673.e

Efflux system outer membrane protein

CENTER ID: BuceA.01673.e
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01673.e.A2.GE34654 Mature protein( BuceA.01673.e ) 21 507
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01673.e.A2.PW35264 Mature protein( BuceA.01673.e ) 21 507

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2919
RefSeq: YP_002231922.1
UniProt: B4EA04

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMQKHALTAI AVALFATGCT MAPHYKRPDA PVAQAYPASG VYATQPGAAG ARSANGQAAT AIGWREFFVD PRLQRLIEIA LKNNRDLRVS VLNIEAARAQ YQITRAGLFP TLDGTGTGNR QRLPNSLTAV PGRNITTTYN VGLSASWELD LFGRVQSLKD QALAQYLSTS YARQASEISL VSQVADQYLT LLSTDDLLKV TEDTLKTAQA SYDLTKLQFD NGTGSELELR QAQTVVETAL ANQQAQARAR AQALNALVLL IGEPLPDDLP AGMPLDAQNL LTDVPAGLPS DLLTRRPDVM QAEQTLLAAN ANIGAARAAF FPKISLTGAF GTASPTLGGL FKAGTAAWSF APSIALPIFE GGQNIANLDL ANVQKRIEIA NYEKAIQSAF REVSDGLAAR GTYDQQIAAL ERNEHAQQRR FDLSDLRYKN GVDSYLSVLT AQTDLYAAQQ SLISARLARW TNLVDLYRAL GGGWIQRAGE TPRAPDAPVD YDKAAAPAPA SATATNG
NT Sequence
ATGATGCAAA AACACGCTTT GACTGCAATC GCAGTCGCGC TCTTTGCCAC GGGCTGCACG ATGGCGCCGC ACTACAAGCG GCCCGACGCA CCCGTCGCGC AGGCGTACCC GGCCAGCGGC GTCTACGCGA CGCAGCCGGG CGCGGCCGGC GCGCGCAGCG CGAATGGCCA GGCGGCGACT GCCATCGGCT GGCGCGAATT CTTCGTCGAT CCGCGCCTGC AGCGGCTGAT CGAGATCGCG CTGAAAAACA ACCGAGACCT GCGCGTATCG GTGCTCAATA TCGAGGCCGC GCGTGCGCAG TACCAGATCA CGCGTGCGGG GCTGTTCCCG ACGCTCGACG GTACGGGTAC GGGCAACCGT CAGCGTTTGC CGAATTCGCT GACGGCCGTG CCGGGCCGGA ACATCACGAC GACCTACAAC GTCGGGCTGT CCGCGTCATG GGAACTCGAC CTGTTCGGCC GCGTGCAGAG CCTGAAGGAT CAGGCGCTCG CGCAATACCT GTCGACGTCG TACGCGCGGC AGGCGTCCGA GATCTCGCTG GTGTCGCAGG TCGCGGATCA ATATCTGACG CTGCTGTCGA CCGATGATCT GCTGAAGGTC ACGGAGGACA CGCTGAAGAC CGCGCAGGCA TCGTACGACC TGACCAAGCT GCAGTTCGAC AACGGCACGG GCTCCGAGCT CGAGCTGCGT CAGGCGCAGA CGGTGGTCGA GACGGCGCTC GCGAACCAGC AGGCGCAGGC GCGAGCCCGC GCGCAGGCGC TGAACGCGCT GGTGCTGCTG ATCGGCGAGC CGCTGCCGGA CGATCTGCCG GCGGGCATGC CGCTCGATGC GCAGAACCTG CTGACGGACG TGCCGGCCGG GTTGCCGTCG GACCTGCTGA CGCGTCGTCC TGACGTGATG CAGGCCGAGC AGACGCTGCT GGCCGCGAAT GCGAACATCG GCGCGGCGCG CGCGGCGTTC TTCCCGAAGA TTTCGCTGAC GGGCGCGTTC GGCACCGCGA GCCCGACGCT GGGCGGCCTG TTCAAGGCCG GCACGGCGGC GTGGTCGTTC GCGCCGAGCA TCGCGCTGCC GATCTTCGAA GGCGGGCAGA ACATCGCGAA CCTCGATCTC GCGAACGTGC AGAAGCGCAT CGAGATCGCG AACTACGAGA AGGCGATCCA GTCGGCGTTC CGCGAAGTGT CGGATGGTCT GGCCGCACGC GGCACGTACG ACCAGCAGAT CGCGGCGCTC GAGCGCAACG AGCACGCGCA GCAGCGACGC TTCGATCTGT CGGACCTGCG CTACAAGAAC GGTGTCGACA GCTATCTGTC GGTGCTGACC GCGCAGACGG ATCTGTACGC CGCGCAGCAG TCGCTGATCA GCGCACGTCT GGCACGCTGG ACGAATCTGG TCGACCTGTA CCGCGCACTG GGCGGCGGGT GGATCCAGCG CGCCGGCGAG ACGCCGCGCG CGCCGGATGC GCCGGTCGAT TACGACAAGG CGGCTGCGCC GGCGCCCGCG TCGGCAACCG CGACGAACGG G
Details for BuceA.01673.e.A2.GE34654
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 54kDa
OBSERVED MW: 54kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Validated AA Sequence
MAPHYKRPDA PVAQAYPASG VYATQPGAAG ARSANGQAAT AIGWREFFVD PRLQRLIEIA LKNNRDLRVS VLNIEAARAQ YQITRAGLFP TLDGTGTGNR QRLPNSLTAV PGRNITTTYN VGLSASWELD LFGRVQSLKD QALAQYLSTS YARQASEISL VSQVADQYLT LLSTDXLLKV TEDTLKTAQA SYDLTKLQFD NGTGSELELR QAQTVVETAL ANQQAQARAR AQALNALVLL IGEPLPDDLP AGMPLDAQNL LTDVPAGLPS DLLTRRPDVM QAEQTLLAAN ANIGAARAAF FPKISLTGAF GTASPTLGGL FKAGTAAWSF APSIALPIFE GGQNIANLDL ANVQKRIEIA NYEKAIQSAF REVSDGLAAR GTYDQQIAAL ERNEHAQQRR FDLSDLRYKN GVDSYLSVLT AQTDLYAAQQ SLISARLARW TNLVDLYRAL GGGWIQRAGE TPRAPDAPVD YDKAAAPAPA SATAT
Validated NT Sequence
tcgtcgcggt tgccgacgcg ggcgccggcg cagccgcctt gtcgtaatcg accggcgcat ccggcgcgcg cggcgtctcg ccggcgcgct ggatccaccc gccgcccagt gcgcggtaca ggtcgaccag attcgtccag cgtgccagac gtgcgctgat cagcgactgc tgcgcggcgt acagatccgt ctgcgcggtc agcaccgaca gatagctgtc gacaccgttc ttgtagcgca ggtccgacag atcgaagcgt cgctgctgcg cgtgctcgtt gcgctcgagc gccgcgatct gctggtcgta cgtgccgcgt gcggccagac catccgacac ttcgcggaac gccgactgga tcgccttctc gtagttcgcg atctcgatgc gcttctgcac gttcgcgaga tcgaggttcg cgatgttctg cccgccttcg aagatcggca gcgcgatgct cggcgcgaac gaccacgccg ccgtgccggc cttgaacagg ccgcccagcg tcgggctcgc ggtgccgaac gcgcccgtca gcgaaatctt cgggaagaac gccgcgcgcg ccgcgccgat gttcgcattc gcggccagca gcgtctgctc ggcctgcatc acgtcaggac gacgcgtcag caggtccgac ggcaacccgg ccggcacgtc cgtcagcagg ttctgcgcat cgagcggcat gcccgccggc agatcgtccg gcagcggctc gccgatcagc agcaccagcg cgttcagcgc ctgcgcgcgg gctcgcgcct gcgcctgctg gttcgcgagc gccgtctcga ccaccgtctg cgcctgacgc agctcgagct cggagcccgt gccgttgtcg aactgcagct tggtcaggtc gtacgatgcc tgcgcggtct tcagcgtgtc ctccgtgacc ttcagcagnt catcggtcga cagcagcgtc agatattgat ccgcgacctg cgacaccagc gagatctcgg acgcctgccg cgcgtacgac gtcgacaggt attgcgcgag cgcctgatcc ttcaggctct gcacgcggcc gaacaggtcg agttcccatg acgcggacag cccgacgttg taggtcgtcg tgatgttccg gcccggcacg gccgtcagcg aattcggcaa acgctgacgg ttgcccgtac ccgtaccgtc gagcgtcggg aacagccccg cacgcgtgat ctggtactgc gcacgcgcgg cctcgatatt gagcaccgat acgcgcaggt ctcggttgtt tttcagcgcg atctcgatca gccgctgcag gcgcggatcg acgaagaatt cgcgccagcc gatggcagtc gccgcctggc cattcgcgct gcgcgcgccg gccgcgcccg gctgcgtcgc gtagacgccg ctggccgggt acgcctgcgc gacgggtgcg tcgggccgct tgtagtgcgg cgccatcgaa ccaggaccct gggtctgagc ttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPHYKRPD APVAQAYPAS GVYATQPGAA GARSANGQAA TAIGWREFFV DPRLQRLIEI ALKNNRDLRV SVLNIEAARA QYQITRAGLF PTLDGTGTGN RQRLPNSLTA VPGRNITTTY NVGLSASWEL DLFGRVQSLK DQALAQYLST SYARQASEIS LVSQVADQYL TLLSTDDLLK VTEDTLKTAQ ASYDLTKLQF DNGTGSELEL RQAQTVVETA LANQQAQARA RAQALNALVL LIGEPLPDDL PAGMPLDAQN LLTDVPAGLP SDLLTRRPDV MQAEQTLLAA NANIGAARAA FFPKISLTGA FGTASPTLGG LFKAGTAAWS FAPSIALPIF EGGQNIANLD LANVQKRIEI ANYEKAIQSA FREVSDGLAA RGTYDQQIAA LERNEHAQQR RFDLSDLRYK NGVDSYLSVL TAQTDLYAAQ QSLISARLAR WTNLVDLYRA LGGGWIQRAG ETPRAPDAPV DYDKAAAPAP ASATATNG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgccgcac tacaagcggc ccgacgcacc cgtcgcgcag gcgtacccgg ccagcggcgt ctacgcgacg cagccgggcg cggccggcgc gcgcagcgcg aatggccagg cggcgactgc catcggctgg cgcgaattct tcgtcgatcc gcgcctgcag cggctgatcg agatcgcgct gaaaaacaac cgagacctgc gcgtatcggt gctcaatatc gaggccgcgc gtgcgcagta ccagatcacg cgtgcggggc tgttcccgac gctcgacggt acgggtacgg gcaaccgtca gcgtttgccg aattcgctga cggccgtgcc gggccggaac atcacgacga cctacaacgt cgggctgtcc gcgtcatggg aactcgacct gttcggccgc gtgcagagcc tgaaggatca ggcgctcgcg caatacctgt cgacgtcgta cgcgcggcag gcgtccgaga tctcgctggt gtcgcaggtc gcggatcaat atctgacgct gctgtcgacc gatgatctgc tgaaggtcac ggaggacacg ctgaagaccg cgcaggcatc gtacgacctg accaagctgc agttcgacaa cggcacgggc tccgagctcg agctgcgtca ggcgcagacg gtggtcgaga cggcgctcgc gaaccagcag gcgcaggcgc gagcccgcgc gcaggcgctg aacgcgctgg tgctgctgat cggcgagccg ctgccggacg atctgccggc gggcatgccg ctcgatgcgc agaacctgct gacggacgtg ccggccgggt tgccgtcgga cctgctgacg cgtcgtcctg acgtgatgca ggccgagcag acgctgctgg ccgcgaatgc gaacatcggc gcggcgcgcg cggcgttctt cccgaagatt tcgctgacgg gcgcgttcgg caccgcgagc ccgacgctgg gcggcctgtt caaggccggc acggcggcgt ggtcgttcgc gccgagcatc gcgctgccga tcttcgaagg cgggcagaac atcgcgaacc tcgatctcgc gaacgtgcag aagcgcatcg agatcgcgaa ctacgagaag gcgatccagt cggcgttccg cgaagtgtcg gatggtctgg ccgcacgcgg cacgtacgac cagcagatcg cggcgctcga gcgcaacgag cacgcgcagc agcgacgctt cgatctgtcg gacctgcgct acaagaacgg tgtcgacagc tatctgtcgg tgctgaccgc gcagacggat ctgtacgccg cgcagcagtc gctgatcagc gcacgtctgg cacgctggac gaatctggtc gacctgtacc gcgcactggg cggcgggtgg atccagcgcg ccggcgagac gccgcgcgcg ccggatgcgc cggtcgatta cgacaaggcg gctgcgccgg cgcccgcgtc ggcaaccgcg acgaacgggt aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa
Details for BuceA.01673.e.A2.PW35264
PURIFICATION DATe: 4/13/2012
CONCENTRATION: 24.95mg/ml
OBSERVED MW: 50kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 15
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MAPHYKRPDA PVAQAYPASG VYATQPGAAG ARSANGQAAT AIGWREFFVD PRLQRLIEIA LKNNRDLRVS VLNIEAARAQ YQITRAGLFP TLDGTGTGNR QRLPNSLTAV PGRNITTTYN VGLSASWELD LFGRVQSLKD QALAQYLSTS YARQASEISL VSQVADQYLT LLSTDXLLKV TEDTLKTAQA SYDLTKLQFD NGTGSELELR QAQTVVETAL ANQQAQARAR AQALNALVLL IGEPLPDDLP AGMPLDAQNL LTDVPAGLPS DLLTRRPDVM QAEQTLLAAN ANIGAARAAF FPKISLTGAF GTASPTLGGL FKAGTAAWSF APSIALPIFE GGQNIANLDL ANVQKRIEIA NYEKAIQSAF REVSDGLAAR GTYDQQIAAL ERNEHAQQRR FDLSDLRYKN GVDSYLSVLT AQTDLYAAQQ SLISARLARW TNLVDLYRAL GGGWIQRAGE TPRAPDAPVD YDKAAAPAPA SATAT
Validated NT Sequence
tcgtcgcggt tgccgacgcg ggcgccggcg cagccgcctt gtcgtaatcg accggcgcat ccggcgcgcg cggcgtctcg ccggcgcgct ggatccaccc gccgcccagt gcgcggtaca ggtcgaccag attcgtccag cgtgccagac gtgcgctgat cagcgactgc tgcgcggcgt acagatccgt ctgcgcggtc agcaccgaca gatagctgtc gacaccgttc ttgtagcgca ggtccgacag atcgaagcgt cgctgctgcg cgtgctcgtt gcgctcgagc gccgcgatct gctggtcgta cgtgccgcgt gcggccagac catccgacac ttcgcggaac gccgactgga tcgccttctc gtagttcgcg atctcgatgc gcttctgcac gttcgcgaga tcgaggttcg cgatgttctg cccgccttcg aagatcggca gcgcgatgct cggcgcgaac gaccacgccg ccgtgccggc cttgaacagg ccgcccagcg tcgggctcgc ggtgccgaac gcgcccgtca gcgaaatctt cgggaagaac gccgcgcgcg ccgcgccgat gttcgcattc gcggccagca gcgtctgctc ggcctgcatc acgtcaggac gacgcgtcag caggtccgac ggcaacccgg ccggcacgtc cgtcagcagg ttctgcgcat cgagcggcat gcccgccggc agatcgtccg gcagcggctc gccgatcagc agcaccagcg cgttcagcgc ctgcgcgcgg gctcgcgcct gcgcctgctg gttcgcgagc gccgtctcga ccaccgtctg cgcctgacgc agctcgagct cggagcccgt gccgttgtcg aactgcagct tggtcaggtc gtacgatgcc tgcgcggtct tcagcgtgtc ctccgtgacc ttcagcagnt catcggtcga cagcagcgtc agatattgat ccgcgacctg cgacaccagc gagatctcgg acgcctgccg cgcgtacgac gtcgacaggt attgcgcgag cgcctgatcc ttcaggctct gcacgcggcc gaacaggtcg agttcccatg acgcggacag cccgacgttg taggtcgtcg tgatgttccg gcccggcacg gccgtcagcg aattcggcaa acgctgacgg ttgcccgtac ccgtaccgtc gagcgtcggg aacagccccg cacgcgtgat ctggtactgc gcacgcgcgg cctcgatatt gagcaccgat acgcgcaggt ctcggttgtt tttcagcgcg atctcgatca gccgctgcag gcgcggatcg acgaagaatt cgcgccagcc gatggcagtc gccgcctggc cattcgcgct gcgcgcgccg gccgcgcccg gctgcgtcgc gtagacgccg ctggccgggt acgcctgcgc gacgggtgcg tcgggccgct tgtagtgcgg cgccatcgaa ccaggaccct gggtctgagc ttccag
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAPHYKRPD APVAQAYPAS GVYATQPGAA GARSANGQAA TAIGWREFFV DPRLQRLIEI ALKNNRDLRV SVLNIEAARA QYQITRAGLF PTLDGTGTGN RQRLPNSLTA VPGRNITTTY NVGLSASWEL DLFGRVQSLK DQALAQYLST SYARQASEIS LVSQVADQYL TLLSTDDLLK VTEDTLKTAQ ASYDLTKLQF DNGTGSELEL RQAQTVVETA LANQQAQARA RAQALNALVL LIGEPLPDDL PAGMPLDAQN LLTDVPAGLP SDLLTRRPDV MQAEQTLLAA NANIGAARAA FFPKISLTGA FGTASPTLGG LFKAGTAAWS FAPSIALPIF EGGQNIANLD LANVQKRIEI ANYEKAIQSA FREVSDGLAA RGTYDQQIAA LERNEHAQQR RFDLSDLRYK NGVDSYLSVL TAQTDLYAAQ QSLISARLAR WTNLVDLYRA LGGGWIQRAG ETPRAPDAPV DYDKAAAPAP ASATATNG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGCGCCGCAC TACAAGCGGC CCGACGCACC CGTCGCGCAG GCGTACCCGG CCAGCGGCGT CTACGCGACG CAGCCGGGCG CGGCCGGCGC GCGCAGCGCG AATGGCCAGG CGGCGACTGC CATCGGCTGG CGCGAATTCT TCGTCGATCC GCGCCTGCAG CGGCTGATCG AGATCGCGCT GAAAAACAAC CGAGACCTGC GCGTATCGGT GCTCAATATC GAGGCCGCGC GTGCGCAGTA CCAGATCACG CGTGCGGGGC TGTTCCCGAC GCTCGACGGT ACGGGTACGG GCAACCGTCA GCGTTTGCCG AATTCGCTGA CGGCCGTGCC GGGCCGGAAC ATCACGACGA CCTACAACGT CGGGCTGTCC GCGTCATGGG AACTCGACCT GTTCGGCCGC GTGCAGAGCC TGAAGGATCA GGCGCTCGCG CAATACCTGT CGACGTCGTA CGCGCGGCAG GCGTCCGAGA TCTCGCTGGT GTCGCAGGTC GCGGATCAAT ATCTGACGCT GCTGTCGACC GATGATCTGC TGAAGGTCAC GGAGGACACG CTGAAGACCG CGCAGGCATC GTACGACCTG ACCAAGCTGC AGTTCGACAA CGGCACGGGC TCCGAGCTCG AGCTGCGTCA GGCGCAGACG GTGGTCGAGA CGGCGCTCGC GAACCAGCAG GCGCAGGCGC GAGCCCGCGC GCAGGCGCTG AACGCGCTGG TGCTGCTGAT CGGCGAGCCG CTGCCGGACG ATCTGCCGGC GGGCATGCCG CTCGATGCGC AGAACCTGCT GACGGACGTG CCGGCCGGGT TGCCGTCGGA CCTGCTGACG CGTCGTCCTG ACGTGATGCA GGCCGAGCAG ACGCTGCTGG CCGCGAATGC GAACATCGGC GCGGCGCGCG CGGCGTTCTT CCCGAAGATT TCGCTGACGG GCGCGTTCGG CACCGCGAGC CCGACGCTGG GCGGCCTGTT CAAGGCCGGC ACGGCGGCGT GGTCGTTCGC GCCGAGCATC GCGCTGCCGA TCTTCGAAGG CGGGCAGAAC ATCGCGAACC TCGATCTCGC GAACGTGCAG AAGCGCATCG AGATCGCGAA CTACGAGAAG GCGATCCAGT CGGCGTTCCG CGAAGTGTCG GATGGTCTGG CCGCACGCGG CACGTACGAC CAGCAGATCG CGGCGCTCGA GCGCAACGAG CACGCGCAGC AGCGACGCTT CGATCTGTCG GACCTGCGCT ACAAGAACGG TGTCGACAGC TATCTGTCGG TGCTGACCGC GCAGACGGAT CTGTACGCCG CGCAGCAGTC GCTGATCAGC GCACGTCTGG CACGCTGGAC GAATCTGGTC GACCTGTACC GCGCACTGGG CGGCGGGTGG ATCCAGCGCG CCGGCGAGAC GCCGCGCGCG CCGGATGCGC CGGTCGATTA CGACAAGGCG GCTGCGCCGG CGCCCGCGTC GGCAACCGCG ACGAACGGGT AAACAGCACG AACAAGTTCT GCAGCCAAGC TTCTCGAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AA