BuceA.01673.d

Multidrug efflux system outer membrane protein

CENTER ID: BuceA.01673.d
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: crystallized
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01673.d.A2.GE34651 Mature protein( BuceA.01673.d ) 28 487
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01673.d.A2.PS01498 Mature protein( BuceA.01673.d ) 28 487
BuceA.01673.d.A2.PS01499 Mature protein( BuceA.01673.d ) 28 487

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1733
RefSeq: YP_002230802.1
UniProt: B4E8U8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MMFALNARGA LRVQVALAAA LALAGCSLAP RYERPAAPVP ATYAPVDAGT ASAAEPAAAQ DAALLDDWHA YFTDPALQAW IDAALANNRD LRIAAGRLDE ARALYGVQRA DRMPSVDANL GYERARQYDP VVRESAISGL YRAGVGVSAY ELDLFGRVRN LSDAALAEYF ATADAQRTVR IGVIAEVAGA YVSERSLHEQ LALAQRTLEA RERMAALTQR RYAAGTSDAI ELRSAEMLVA SARASQAALQ REHAQAIRAL QLLAGDFARN VPDDATALDT LSIAPVAPGA PSQLLERRPD IRQAEARLKA ANANIGAARA AFFPRIALTT DYGSVSDAFS NLFAAGTSVW TFAPRITLPI FAGGRNRANL DVAHARKDIA VAEYEKAVQT AFREVADAFA ARDWIDRQLA AQQDVYAADG ARLKLAERRY AGGVATYLEL LDAQRSTYES GQELIRLRQL RLANAIALYR ALGGGWAPAT AEAAASV
NT Sequence
ATGATGTTTG CGCTGAATGC ACGCGGCGCG CTGCGGGTTC AGGTCGCGCT GGCCGCCGCG CTGGCGCTTG CCGGCTGTTC GCTCGCGCCG CGTTACGAGC GTCCGGCCGC GCCGGTGCCG GCGACCTATG CGCCGGTCGA CGCCGGCACG GCATCGGCGG CCGAACCGGC CGCGGCGCAG GATGCCGCGT TGCTCGACGA CTGGCATGCG TATTTCACCG ATCCGGCGCT GCAGGCGTGG ATCGACGCGG CGCTCGCGAA CAACCGCGAT CTGCGGATCG CGGCCGGCCG GCTCGACGAA GCGCGCGCGC TGTATGGCGT GCAGCGCGCG GACCGGATGC CGTCGGTCGA TGCGAATCTC GGCTATGAAC GCGCGCGCCA GTACGACCCG GTCGTGCGCG AAAGCGCGAT CAGCGGGCTG TATCGCGCGG GCGTTGGTGT CAGTGCGTAC GAGCTCGACC TGTTCGGCCG CGTGCGCAAC CTGTCCGACG CGGCGCTCGC CGAATACTTC GCGACGGCCG ACGCGCAGCG CACGGTCCGC ATCGGCGTGA TCGCCGAAGT GGCGGGCGCG TATGTTTCCG AGCGATCGTT GCACGAGCAA CTGGCGCTCG CTCAGCGTAC GCTCGAAGCC CGCGAGCGGA TGGCCGCGCT CACGCAACGC CGCTATGCCG CGGGCACGAG CGACGCGATC GAGCTGCGCT CGGCCGAGAT GCTGGTGGCG TCCGCGCGCG CGTCGCAGGC CGCGCTGCAG CGCGAGCATG CGCAGGCCAT ACGGGCGCTG CAATTGCTGG CGGGCGATTT CGCGCGCAAC GTGCCCGACG ACGCGACCGC GCTCGACACG CTGTCGATCG CGCCCGTGGC GCCGGGCGCA CCGAGCCAAT TGCTCGAACG ACGGCCGGAC ATCCGGCAGG CCGAGGCACG GCTGAAGGCC GCGAACGCGA ACATCGGCGC GGCGCGTGCG GCGTTCTTCC CGCGCATCGC GCTGACGACC GACTACGGCT CGGTGAGCGA CGCGTTCTCG AACCTGTTCG CGGCCGGCAC GAGCGTGTGG ACGTTCGCGC CGCGCATCAC GCTGCCGATC TTCGCGGGCG GACGCAATCG CGCGAACCTC GACGTCGCGC ACGCGCGCAA GGACATCGCG GTCGCCGAAT ACGAGAAGGC CGTACAGACC GCGTTTCGCG AAGTGGCCGA TGCGTTCGCC GCGCGCGACT GGATCGACCG GCAGCTTGCC GCGCAGCAGG ACGTGTATGC GGCGGACGGC GCGCGGCTGA AGCTCGCGGA GCGTCGCTAT GCGGGCGGCG TCGCGACGTA TCTCGAACTG CTCGACGCGC AGCGCAGCAC GTACGAGTCG GGGCAGGAGC TGATCCGGCT CAGGCAGCTC AGGCTCGCGA ACGCGATCGC GCTGTACCGT GCGCTCGGCG GCGGCTGGGC GCCGGCAACG GCGGAGGCCG CGGCTTCCGT G
Details for BuceA.01673.d.A2.GE34651
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 51kDa
OBSERVED MW: 51kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MLAPRYERPA APVPATYAPV DAGTASAAEP AAAQDAALLD DWHAYFTDPA LQAWIDAALA NNRDLRIAAG RLDEARALYG VQRADRMPSV DANLGYERAR QYDPVVRESA ISGLYRAGVG VSAYELDLFG RVRNLSDAAL AEYFATADAQ RTVRIGVIAE VAGAYVSERS LHEQLALAQR TLEARERMAA LTQRRYAAGT SDAIELRSAE MLVASARASQ AALQREHAQA IRALQLLAGD FARNVPDDAT ALDTLSIAPV APGAPSQLLE RRPDIRQAEA RLKAANANIG AARAAFFPRI ALTTDYGSVS DAFSNLFAAG TSVWTFAPRI TLPIFAGGRN RANLDVAHAR KDIAVAEYEK AVQTAFREVA DAFAARDWID RQLAAQQDVY AADGARLKLA ERRYAGGVAT YLELLDAQRS TYESGQELIR LRQLRLANAI ALYRALGGGW APATAEAAAS
Validated NT Sequence
cacggaagcc gcggcctccg ccgttgccgg cgcccagccg ccgccgagcg cacggtacag cgcgatcgcg ttcgcgagcc tgagctgcct gagccggatc agctcctgcc ccgactcgta cgtgctgcgc tgcgcgtcga gcagttcgag atacgtcgcg acgccgcccg catagcgacg ctccgcgagc ttcagccgcg cgccgtccgc cgcatacacg tcctgctgcg cggcaagctg ccggtcgatc cagtcgcgcg cggcgaacgc atcggccact tcgcgaaacg cggtctgtac ggccttctcg tattcggcga ccgcgatgtc cttgcgcgcg tgcgcgacgt cgaggttcgc gcgattgcgt ccgcccgcga agatcggcag cgtgatgcgc ggcgcgaacg tccacacgct cgtgccggcc gcgaacaggt tcgagaacgc gtcgctcacc gagccgtagt cggtcgtcag cgcgatgcgc gggaagaacg ccgcacgcgc cgcgccgatg ttcgcgttcg cggccttcag ccgtgcctcg gcctgccgga tgtccggccg tcgttcgagc aattggctcg gtgcgcccgg cgccacgggc gcgatcgaca gcgtgtcgag cgcggtcgcg tcgtcgggca cgttgcgcgc gaaatcgccc gccagcaatt gcagcgcccg tatggcctgc gcatgctcgc gctgcagcgc ggcctgcgac gcgcgcgcgg acgccaccag catctcggcc gagcgcagct cgatcgcgtc gctcgtgccc gcggcatagc ggcgttgcgt gagcgcggcc atccgctcgc gggcttcgag cgtacgctga gcgagcgcca gttgctcgtg caacgatcgc tcggaaacat acgcgcccgc cacttcggcg atcacgccga tgcggaccgt gcgctgcgcg tcggccgtcg cgaagtattc ggcgagcgcc gcgtcggaca ggttgcgcac gcggccgaac aggtcgagct cgtacgcact gacaccaacg cccgcgcgat acagcccgct gatcgcgctt tcgcgcacga ccgggtcgta ctggcgcgcg cgttcatagc cgagattcgc atcgaccgac ggcatccggt ccgcgcgctg cacgccatac agcgcgcgcg cttcgtcgag ccggccggcc gcgatccgca gatcgcggtt gttcgcgagc gccgcgtcga tccacgcctg cagcgccgga tcggtgaaat acgcatgcca gtcgtcgagc aacgcggcat cctgcgccgc ggccggttcg gccgccgatg ccgtgccggc gtcgaccggc gcataggtcg ccggcaccgg cgcggccgga cgctcgtaac gcggcgcgag catcgaacca ggaccctggg tctgagcttc cagg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLAPRYERP AAPVPATYAP VDAGTASAAE PAAAQDAALL DDWHAYFTDP ALQAWIDAAL ANNRDLRIAA GRLDEARALY GVQRADRMPS VDANLGYERA RQYDPVVRES AISGLYRAGV GVSAYELDLF GRVRNLSDAA LAEYFATADA QRTVRIGVIA EVAGAYVSER SLHEQLALAQ RTLEARERMA ALTQRRYAAG TSDAIELRSA EMLVASARAS QAALQREHAQ AIRALQLLAG DFARNVPDDA TALDTLSIAP VAPGAPSQLL ERRPDIRQAE ARLKAANANI GAARAAFFPR IALTTDYGSV SDAFSNLFAA GTSVWTFAPR ITLPIFAGGR NRANLDVAHA RKDIAVAEYE KAVQTAFREV ADAFAARDWI DRQLAAQQDV YAADGARLKL AERRYAGGVA TYLELLDAQR STYESGQELI RLRQLRLANA IALYRALGGG WAPATAEAAA SV
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gctcgcgccg cgttacgagc gtccggccgc gccggtgccg gcgacctatg cgccggtcga cgccggcacg gcatcggcgg ccgaaccggc cgcggcgcag gatgccgcgt tgctcgacga ctggcatgcg tatttcaccg atccggcgct gcaggcgtgg atcgacgcgg cgctcgcgaa caaccgcgat ctgcggatcg cggccggccg gctcgacgaa gcgcgcgcgc tgtatggcgt gcagcgcgcg gaccggatgc cgtcggtcga tgcgaatctc ggctatgaac gcgcgcgcca gtacgacccg gtcgtgcgcg aaagcgcgat cagcgggctg tatcgcgcgg gcgttggtgt cagtgcgtac gagctcgacc tgttcggccg cgtgcgcaac ctgtccgacg cggcgctcgc cgaatacttc gcgacggccg acgcgcagcg cacggtccgc atcggcgtga tcgccgaagt ggcgggcgcg tatgtttccg agcgatcgtt gcacgagcaa ctggcgctcg ctcagcgtac gctcgaagcc cgcgagcgga tggccgcgct cacgcaacgc cgctatgccg cgggcacgag cgacgcgatc gagctgcgct cggccgagat gctggtggcg tccgcgcgcg cgtcgcaggc cgcgctgcag cgcgagcatg cgcaggccat acgggcgctg caattgctgg cgggcgattt cgcgcgcaac gtgcccgacg acgcgaccgc gctcgacacg ctgtcgatcg cgcccgtggc gccgggcgca ccgagccaat tgctcgaacg acggccggac atccggcagg ccgaggcacg gctgaaggcc gcgaacgcga acatcggcgc ggcgcgtgcg gcgttcttcc cgcgcatcgc gctgacgacc gactacggct cggtgagcga cgcgttctcg aacctgttcg cggccggcac gagcgtgtgg acgttcgcgc cgcgcatcac gctgccgatc ttcgcgggcg gacgcaatcg cgcgaacctc gacgtcgcgc acgcgcgcaa ggacatcgcg gtcgccgaat acgagaaggc cgtacagacc gcgtttcgcg aagtggccga tgcgttcgcc gcgcgcgact ggatcgaccg gcagcttgcc gcgcagcagg acgtgtatgc ggcggacggc gcgcggctga agctcgcgga gcgtcgctat gcgggcggcg tcgcgacgta tctcgaactg ctcgacgcgc agcgcagcac gtacgagtcg gggcaggagc tgatccggct caggcagctc aggctcgcga acgcgatcgc gctgtaccgt gcgctcggcg gcggctgggc gccggcaacg gcggaggccg cggcttccgt gtaaacagca cgaacaagtt ctgcagccaa gcttctcgag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaa
Details for BuceA.01673.d.A2.PS01498
PURIFICATION DATe: 5/17/2012
CONCENTRATION: 6.83mg/ml
OBSERVED MW: 50kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MLAPRYERPA APVPATYAPV DAGTASAAEP AAAQDAALLD DWHAYFTDPA LQAWIDAALA NNRDLRIAAG RLDEARALYG VQRADRMPSV DANLGYERAR QYDPVVRESA ISGLYRAGVG VSAYELDLFG RVRNLSDAAL AEYFATADAQ RTVRIGVIAE VAGAYVSERS LHEQLALAQR TLEARERMAA LTQRRYAAGT SDAIELRSAE MLVASARASQ AALQREHAQA IRALQLLAGD FARNVPDDAT ALDTLSIAPV APGAPSQLLE RRPDIRQAEA RLKAANANIG AARAAFFPRI ALTTDYGSVS DAFSNLFAAG TSVWTFAPRI TLPIFAGGRN RANLDVAHAR KDIAVAEYEK AVQTAFREVA DAFAARDWID RQLAAQQDVY AADGARLKLA ERRYAGGVAT YLELLDAQRS TYESGQELIR LRQLRLANAI ALYRALGGGW APATAEAAAS
Validated NT Sequence
cacggaagcc gcggcctccg ccgttgccgg cgcccagccg ccgccgagcg cacggtacag cgcgatcgcg ttcgcgagcc tgagctgcct gagccggatc agctcctgcc ccgactcgta cgtgctgcgc tgcgcgtcga gcagttcgag atacgtcgcg acgccgcccg catagcgacg ctccgcgagc ttcagccgcg cgccgtccgc cgcatacacg tcctgctgcg cggcaagctg ccggtcgatc cagtcgcgcg cggcgaacgc atcggccact tcgcgaaacg cggtctgtac ggccttctcg tattcggcga ccgcgatgtc cttgcgcgcg tgcgcgacgt cgaggttcgc gcgattgcgt ccgcccgcga agatcggcag cgtgatgcgc ggcgcgaacg tccacacgct cgtgccggcc gcgaacaggt tcgagaacgc gtcgctcacc gagccgtagt cggtcgtcag cgcgatgcgc gggaagaacg ccgcacgcgc cgcgccgatg ttcgcgttcg cggccttcag ccgtgcctcg gcctgccgga tgtccggccg tcgttcgagc aattggctcg gtgcgcccgg cgccacgggc gcgatcgaca gcgtgtcgag cgcggtcgcg tcgtcgggca cgttgcgcgc gaaatcgccc gccagcaatt gcagcgcccg tatggcctgc gcatgctcgc gctgcagcgc ggcctgcgac gcgcgcgcgg acgccaccag catctcggcc gagcgcagct cgatcgcgtc gctcgtgccc gcggcatagc ggcgttgcgt gagcgcggcc atccgctcgc gggcttcgag cgtacgctga gcgagcgcca gttgctcgtg caacgatcgc tcggaaacat acgcgcccgc cacttcggcg atcacgccga tgcggaccgt gcgctgcgcg tcggccgtcg cgaagtattc ggcgagcgcc gcgtcggaca ggttgcgcac gcggccgaac aggtcgagct cgtacgcact gacaccaacg cccgcgcgat acagcccgct gatcgcgctt tcgcgcacga ccgggtcgta ctggcgcgcg cgttcatagc cgagattcgc atcgaccgac ggcatccggt ccgcgcgctg cacgccatac agcgcgcgcg cttcgtcgag ccggccggcc gcgatccgca gatcgcggtt gttcgcgagc gccgcgtcga tccacgcctg cagcgccgga tcggtgaaat acgcatgcca gtcgtcgagc aacgcggcat cctgcgccgc ggccggttcg gccgccgatg ccgtgccggc gtcgaccggc gcataggtcg ccggcaccgg cgcggccgga cgctcgtaac gcggcgcgag catcgaacca ggaccctggg tctgagcttc cagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLAPRYERP AAPVPATYAP VDAGTASAAE PAAAQDAALL DDWHAYFTDP ALQAWIDAAL ANNRDLRIAA GRLDEARALY GVQRADRMPS VDANLGYERA RQYDPVVRES AISGLYRAGV GVSAYELDLF GRVRNLSDAA LAEYFATADA QRTVRIGVIA EVAGAYVSER SLHEQLALAQ RTLEARERMA ALTQRRYAAG TSDAIELRSA EMLVASARAS QAALQREHAQ AIRALQLLAG DFARNVPDDA TALDTLSIAP VAPGAPSQLL ERRPDIRQAE ARLKAANANI GAARAAFFPR IALTTDYGSV SDAFSNLFAA GTSVWTFAPR ITLPIFAGGR NRANLDVAHA RKDIAVAEYE KAVQTAFREV ADAFAARDWI DRQLAAQQDV YAADGARLKL AERRYAGGVA TYLELLDAQR STYESGQELI RLRQLRLANA IALYRALGGG WAPATAEAAA SV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTCGCGCCG CGTTACGAGC GTCCGGCCGC GCCGGTGCCG GCGACCTATG CGCCGGTCGA CGCCGGCACG GCATCGGCGG CCGAACCGGC CGCGGCGCAG GATGCCGCGT TGCTCGACGA CTGGCATGCG TATTTCACCG ATCCGGCGCT GCAGGCGTGG ATCGACGCGG CGCTCGCGAA CAACCGCGAT CTGCGGATCG CGGCCGGCCG GCTCGACGAA GCGCGCGCGC TGTATGGCGT GCAGCGCGCG GACCGGATGC CGTCGGTCGA TGCGAATCTC GGCTATGAAC GCGCGCGCCA GTACGACCCG GTCGTGCGCG AAAGCGCGAT CAGCGGGCTG TATCGCGCGG GCGTTGGTGT CAGTGCGTAC GAGCTCGACC TGTTCGGCCG CGTGCGCAAC CTGTCCGACG CGGCGCTCGC CGAATACTTC GCGACGGCCG ACGCGCAGCG CACGGTCCGC ATCGGCGTGA TCGCCGAAGT GGCGGGCGCG TATGTTTCCG AGCGATCGTT GCACGAGCAA CTGGCGCTCG CTCAGCGTAC GCTCGAAGCC CGCGAGCGGA TGGCCGCGCT CACGCAACGC CGCTATGCCG CGGGCACGAG CGACGCGATC GAGCTGCGCT CGGCCGAGAT GCTGGTGGCG TCCGCGCGCG CGTCGCAGGC CGCGCTGCAG CGCGAGCATG CGCAGGCCAT ACGGGCGCTG CAATTGCTGG CGGGCGATTT CGCGCGCAAC GTGCCCGACG ACGCGACCGC GCTCGACACG CTGTCGATCG CGCCCGTGGC GCCGGGCGCA CCGAGCCAAT TGCTCGAACG ACGGCCGGAC ATCCGGCAGG CCGAGGCACG GCTGAAGGCC GCGAACGCGA ACATCGGCGC GGCGCGTGCG GCGTTCTTCC CGCGCATCGC GCTGACGACC GACTACGGCT CGGTGAGCGA CGCGTTCTCG AACCTGTTCG CGGCCGGCAC GAGCGTGTGG ACGTTCGCGC CGCGCATCAC GCTGCCGATC TTCGCGGGCG GACGCAATCG CGCGAACCTC GACGTCGCGC ACGCGCGCAA GGACATCGCG GTCGCCGAAT ACGAGAAGGC CGTACAGACC GCGTTTCGCG AAGTGGCCGA TGCGTTCGCC GCGCGCGACT GGATCGACCG GCAGCTTGCC GCGCAGCAGG ACGTGTATGC GGCGGACGGC GCGCGGCTGA AGCTCGCGGA GCGTCGCTAT GCGGGCGGCG TCGCGACGTA TCTCGAACTG CTCGACGCGC AGCGCAGCAC GTACGAGTCG GGGCAGGAGC TGATCCGGCT CAGGCAGCTC AGGCTCGCGA ACGCGATCGC GCTGTACCGT GCGCTCGGCG GCGGCTGGGC GCCGGCAACG GCGGAGGCCG CGGCTTCCGT GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA
Details for BuceA.01673.d.A2.PS01499
PURIFICATION DATe: 5/17/2012
CONCENTRATION: 7.7mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: 110µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MLAPRYERPA APVPATYAPV DAGTASAAEP AAAQDAALLD DWHAYFTDPA LQAWIDAALA NNRDLRIAAG RLDEARALYG VQRADRMPSV DANLGYERAR QYDPVVRESA ISGLYRAGVG VSAYELDLFG RVRNLSDAAL AEYFATADAQ RTVRIGVIAE VAGAYVSERS LHEQLALAQR TLEARERMAA LTQRRYAAGT SDAIELRSAE MLVASARASQ AALQREHAQA IRALQLLAGD FARNVPDDAT ALDTLSIAPV APGAPSQLLE RRPDIRQAEA RLKAANANIG AARAAFFPRI ALTTDYGSVS DAFSNLFAAG TSVWTFAPRI TLPIFAGGRN RANLDVAHAR KDIAVAEYEK AVQTAFREVA DAFAARDWID RQLAAQQDVY AADGARLKLA ERRYAGGVAT YLELLDAQRS TYESGQELIR LRQLRLANAI ALYRALGGGW APATAEAAAS
Validated NT Sequence
cacggaagcc gcggcctccg ccgttgccgg cgcccagccg ccgccgagcg cacggtacag cgcgatcgcg ttcgcgagcc tgagctgcct gagccggatc agctcctgcc ccgactcgta cgtgctgcgc tgcgcgtcga gcagttcgag atacgtcgcg acgccgcccg catagcgacg ctccgcgagc ttcagccgcg cgccgtccgc cgcatacacg tcctgctgcg cggcaagctg ccggtcgatc cagtcgcgcg cggcgaacgc atcggccact tcgcgaaacg cggtctgtac ggccttctcg tattcggcga ccgcgatgtc cttgcgcgcg tgcgcgacgt cgaggttcgc gcgattgcgt ccgcccgcga agatcggcag cgtgatgcgc ggcgcgaacg tccacacgct cgtgccggcc gcgaacaggt tcgagaacgc gtcgctcacc gagccgtagt cggtcgtcag cgcgatgcgc gggaagaacg ccgcacgcgc cgcgccgatg ttcgcgttcg cggccttcag ccgtgcctcg gcctgccgga tgtccggccg tcgttcgagc aattggctcg gtgcgcccgg cgccacgggc gcgatcgaca gcgtgtcgag cgcggtcgcg tcgtcgggca cgttgcgcgc gaaatcgccc gccagcaatt gcagcgcccg tatggcctgc gcatgctcgc gctgcagcgc ggcctgcgac gcgcgcgcgg acgccaccag catctcggcc gagcgcagct cgatcgcgtc gctcgtgccc gcggcatagc ggcgttgcgt gagcgcggcc atccgctcgc gggcttcgag cgtacgctga gcgagcgcca gttgctcgtg caacgatcgc tcggaaacat acgcgcccgc cacttcggcg atcacgccga tgcggaccgt gcgctgcgcg tcggccgtcg cgaagtattc ggcgagcgcc gcgtcggaca ggttgcgcac gcggccgaac aggtcgagct cgtacgcact gacaccaacg cccgcgcgat acagcccgct gatcgcgctt tcgcgcacga ccgggtcgta ctggcgcgcg cgttcatagc cgagattcgc atcgaccgac ggcatccggt ccgcgcgctg cacgccatac agcgcgcgcg cttcgtcgag ccggccggcc gcgatccgca gatcgcggtt gttcgcgagc gccgcgtcga tccacgcctg cagcgccgga tcggtgaaat acgcatgcca gtcgtcgagc aacgcggcat cctgcgccgc ggccggttcg gccgccgatg ccgtgccggc gtcgaccggc gcataggtcg ccggcaccgg cgcggccgga cgctcgtaac gcggcgcgag catcgaacca ggaccctggg tctgagcttc cagg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMLAPRYERP AAPVPATYAP VDAGTASAAE PAAAQDAALL DDWHAYFTDP ALQAWIDAAL ANNRDLRIAA GRLDEARALY GVQRADRMPS VDANLGYERA RQYDPVVRES AISGLYRAGV GVSAYELDLF GRVRNLSDAA LAEYFATADA QRTVRIGVIA EVAGAYVSER SLHEQLALAQ RTLEARERMA ALTQRRYAAG TSDAIELRSA EMLVASARAS QAALQREHAQ AIRALQLLAG DFARNVPDDA TALDTLSIAP VAPGAPSQLL ERRPDIRQAE ARLKAANANI GAARAAFFPR IALTTDYGSV SDAFSNLFAA GTSVWTFAPR ITLPIFAGGR NRANLDVAHA RKDIAVAEYE KAVQTAFREV ADAFAARDWI DRQLAAQQDV YAADGARLKL AERRYAGGVA TYLELLDAQR STYESGQELI RLRQLRLANA IALYRALGGG WAPATAEAAA SV
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCTCGCGCCG CGTTACGAGC GTCCGGCCGC GCCGGTGCCG GCGACCTATG CGCCGGTCGA CGCCGGCACG GCATCGGCGG CCGAACCGGC CGCGGCGCAG GATGCCGCGT TGCTCGACGA CTGGCATGCG TATTTCACCG ATCCGGCGCT GCAGGCGTGG ATCGACGCGG CGCTCGCGAA CAACCGCGAT CTGCGGATCG CGGCCGGCCG GCTCGACGAA GCGCGCGCGC TGTATGGCGT GCAGCGCGCG GACCGGATGC CGTCGGTCGA TGCGAATCTC GGCTATGAAC GCGCGCGCCA GTACGACCCG GTCGTGCGCG AAAGCGCGAT CAGCGGGCTG TATCGCGCGG GCGTTGGTGT CAGTGCGTAC GAGCTCGACC TGTTCGGCCG CGTGCGCAAC CTGTCCGACG CGGCGCTCGC CGAATACTTC GCGACGGCCG ACGCGCAGCG CACGGTCCGC ATCGGCGTGA TCGCCGAAGT GGCGGGCGCG TATGTTTCCG AGCGATCGTT GCACGAGCAA CTGGCGCTCG CTCAGCGTAC GCTCGAAGCC CGCGAGCGGA TGGCCGCGCT CACGCAACGC CGCTATGCCG CGGGCACGAG CGACGCGATC GAGCTGCGCT CGGCCGAGAT GCTGGTGGCG TCCGCGCGCG CGTCGCAGGC CGCGCTGCAG CGCGAGCATG CGCAGGCCAT ACGGGCGCTG CAATTGCTGG CGGGCGATTT CGCGCGCAAC GTGCCCGACG ACGCGACCGC GCTCGACACG CTGTCGATCG CGCCCGTGGC GCCGGGCGCA CCGAGCCAAT TGCTCGAACG ACGGCCGGAC ATCCGGCAGG CCGAGGCACG GCTGAAGGCC GCGAACGCGA ACATCGGCGC GGCGCGTGCG GCGTTCTTCC CGCGCATCGC GCTGACGACC GACTACGGCT CGGTGAGCGA CGCGTTCTCG AACCTGTTCG CGGCCGGCAC GAGCGTGTGG ACGTTCGCGC CGCGCATCAC GCTGCCGATC TTCGCGGGCG GACGCAATCG CGCGAACCTC GACGTCGCGC ACGCGCGCAA GGACATCGCG GTCGCCGAAT ACGAGAAGGC CGTACAGACC GCGTTTCGCG AAGTGGCCGA TGCGTTCGCC GCGCGCGACT GGATCGACCG GCAGCTTGCC GCGCAGCAGG ACGTGTATGC GGCGGACGGC GCGCGGCTGA AGCTCGCGGA GCGTCGCTAT GCGGGCGGCG TCGCGACGTA TCTCGAACTG CTCGACGCGC AGCGCAGCAC GTACGAGTCG GGGCAGGAGC TGATCCGGCT CAGGCAGCTC AGGCTCGCGA ACGCGATCGC GCTGTACCGT GCGCTCGGCG GCGGCTGGGC GCCGGCAACG GCGGAGGCCG CGGCTTCCGT GTAAACAGCA CGAACAAGTT CTGCAGCCAA GCTTCTCGAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAA