SSGCID: Target Page

BuceA.01666.e

Two-component regulatory system, response regulator protein

CENTER ID:	BuceA.01666.e
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01666.e.A1.GE33210	full length		1	225

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.3635
RefSeq:	YP_002232598.1
UniProt:	B4E6M6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MKLLLVEDNA ELAHWIVNLL RGEDFAVDCV GDGERADTVL KTERYDAVLL DMRLPGISGK EVLARLRRRN DNVPVLMLTA HGSVDDKVDC FGAGADDYVV KPFESRELVA RIRALIRRQA GVGTTQLVCG DLVYAFGTRE FRCGDAVLAL RRREHAILET LMLQQNKTVS KARLMDSVFA LDDEPSADAI DIYIHRLRKH LAGSTAEIIT LRGLGYILRM KSAHD

NT Sequence

atgaaacttt tgctggtcga agacaacgcc gagctcgcgc actggatcgt gaacctgctg cgcggggagg atttcgcggt cgactgcgtc ggcgacggcg aacgggccga cacggtgctc aagaccgaac gctacgacgc ggtgctgctc gacatgcgcc tgcccggcat cagcggcaag gaagtgctcg cacggctgcg gcgccgcaac gacaacgtgc cggtgctgat gctgaccgcg cacggctcgg tcgacgacaa ggtcgactgc ttcggcgcgg gcgccgacga ttacgtcgtg aagccgttcg agtcgcgcga gctggtggcg cgcatccgtg cgctgatccg ccggcaggcc ggcgtcggca cgacgcagct cgtgtgcggc gacctcgtct atgcgttcgg tacgcgcgaa ttccgctgcg gcgacgcggt gctcgcgctg cgccgccgcg agcacgcgat cctcgaaacg ctgatgctgc agcagaacaa gacggtgtcg aaggcgcggc tgatggacag cgtgttcgcg ctcgacgacg agcccagcgc cgacgcgatc gacatctaca tccaccggct gcgcaagcat ctcgccggca gcacggccga gatcatcacg ctgcgcgggc tcggttacat cctgcgcatg aagagcgcgc acgattga

Details for BuceA.01666.e.A1.GE33210

HARVESTED ON:	8/30/2011
SEQUENCED ON:	9/2/2011
EXPECTED MW:	27kDa
OBSERVED MW:	27kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMKLLLVEDN AELAHWIVNL LRGEDFAVDC VGDGERADTV LKTERYDAVL LDMRLPGISG KEVLARLRRR NDNVPVLMLT AHGSVDDKVD CFGAGADDYV VKPFESRELV ARIRALIRRQ AGVGTTQLVC GDLVYAFGTR EFRCGDAVLA LRRREHAILE TLMLQQNKTV SKARLMDSVF ALDDEPSADA IDIYIHRLRK HLAGSTAEII TLRGLGYILR MKSAHD

Validated NT Sequence

tnnntttaan aaggagatat accatggctc atcaccatca ccatcatatg ggtaccctgg aagctcagac ccagggtcct ggttcgatga aacttttgct ggtcgaagac aacgccgagc tcgcgcactg gatcgtgaac ctgctgcgcg gggaggattt cgcggtcgac tgcgtcggcg acggcgaacg ggccgacacg gtgctcaaga ccgaacgcta cgacgcggtg ctgctcgaca tgcgcctgcc cggcatcagc ggcaaggaag tgctcgcacg gctgcggcgc cgcaacgaca acgtgccggt gctgatgctg accgcgcacg gctcggtcga cgacaaggtc gactgcttcg gcgcgggcgc cgacgattac gtcgtgaagc cgttcgagtc gcgcgagctg gtggcgcgca tccgtgcgct gatccgccgg caggccggcg tcggcacgac gcagctcgtg tgcggcgacc tcgtctatgc gttcggtacg cgcgaattcc gctgcggcga cgcggtgctc gcgctgcgcc gccgcgagca cgcgatcctc gaaacgctga tgctgcagca gaacaagacg gtgtcgaagg cgcggctgat ggacagcgtg ttcgcgctcg acgacgagcc cagcgccgac gcgatcgaca tctacatcca ccggctgcgc aagcatctcg ccggcagcac ggccgagatc atcacgctgc gcgggctcgg ttacatcctg cgcatgaaga gcgcgcacga ttaataaaca gcacgaacaa gttctgcagc ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccncnnnnnn nnaaaaannn nnnaaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaacttttg ctggtcgaag acaacgccga gctcgcgcac tggatcgtga acctgctgcg cggggaggat ttcgcggtcg actgcgtcgg cgacggcgaa cgggccgaca cggtgctcaa gaccgaacgc tacgacgcgg tgctgctcga catgcgcctg cccggcatca gcggcaagga agtgctcgca cggctgcggc gccgcaacga caacgtgccg gtgctgatgc tgaccgcgca cggctcggtc gacgacaagg tcgactgctt cggcgcgggc gccgacgatt acgtcgtgaa gccgttcgag tcgcgcgagc tggtggcgcg catccgtgcg ctgatccgcc ggcaggccgg cgtcggcacg acgcagctcg tgtgcggcga cctcgtctat gcgttcggta cgcgcgaatt ccgctgcggc gacgcggtgc tcgcgctgcg ccgccgcgag cacgcgatcc tcgaaacgct gatgctgcag cagaacaaga cggtgtcgaa ggcgcggctg atggacagcg tgttcgcgct cgacgacgag cccagcgccg acgcgatcga catctacatc caccggctgc gcaagcatct cgccggcagc acggccgaga tcatcacgct gcgcgggctc ggttacatcc tgcgcatgaa gagcgcgcac gattaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa