BuceA.01666.c

Putative nitrogen regulation protein NR(II)

CENTER ID: BuceA.01666.c
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01666.c.A1.GE31827 Full length( BuceA.01666.c ) 1 380
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2314
RefSeq: YP_002231349.1
UniProt: B4EDV2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVLKNLIKAK TGQPERLTDD ERLSRSGLLA GLEALPTVVI VLDRKTLRIA FANPSAEAML DISRRQLAQR PWGEIFPNAN ELASTITAIG EERFHATHLD TVLDRPGREP LHVHAIVGFL ENAPDFVLVE LFENERQSRT DREERIHDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELGERERGEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ERVRAVMLAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAQAL RERIAQGDAK IELRTRIARK VTIAKRLYRL ALDLHVIDNG PGIPEEIRDR IFYPLVSGRE DGSGLGLTLA QTFVQQHDGM IEVESRPGRT EFQILLPLDH
NT Sequence
ATGGTTCTGA AGAATCTGAT CAAGGCGAAA ACGGGGCAGC CCGAACGACT GACGGACGAC GAGCGGCTCT CGCGCTCGGG TCTGCTGGCG GGGCTCGAAG CGCTGCCGAC GGTCGTGATC GTGCTCGACC GCAAGACGCT GCGGATCGCG TTCGCGAACC CGTCCGCGGA GGCGATGCTC GACATCTCGC GGCGGCAGCT CGCGCAGCGG CCGTGGGGCG AGATTTTTCC GAACGCGAAC GAGCTGGCCT CGACGATCAC CGCGATCGGC GAGGAACGCT TTCACGCGAC GCATCTCGAT ACCGTGCTCG ACCGGCCCGG CCGCGAACCG CTGCACGTGC ATGCGATCGT CGGCTTCCTC GAGAACGCGC CCGATTTCGT GCTCGTCGAG CTGTTCGAGA ACGAACGGCA GTCGCGCACC GATCGGGAAG AGCGCATCCA CGACCTGACC GCGGTCAACA AGCAACTGAT CCGCAACCTC GCGCACGAGA TCAAGAACCC GCTCGGCGGC ATTCGCGGCG CAGCCCAGCT GCTCGAATTC GAGCTGGGCG AGCGCGAGCG CGGCGAGTTG CGCGAATACA CGCAGGTGAT CATCAAGGAG TCCGACCGCC TGCAGACGCT CGTCGACCGG TTGCTGGAGC CGCACCGGCA TCCGCACATC GTCGGCGACG TGAACATTCA TGAAGTGTGC GAACGCGTGC GCGCGGTGAT GCTCGCGGAG TTCCCGCGCG GGCTCACGAT CGAGCGCGAC TACGACGTGA GCGTGCCGGA CCTGCGCGGC GACAAGGAGC AGTTGATCCA GGCGCTGCTC AACATCGTGC GCAACGCCGC GCAGGCGCTG CGCGAACGGA TCGCGCAGGG CGACGCGAAG ATCGAGCTGC GCACGCGCAT CGCGCGCAAG GTGACGATCG CCAAGCGCCT GTACCGGCTG GCACTGGACT TGCACGTGAT CGACAACGGG CCCGGCATTC CGGAAGAGAT CCGCGACCGG ATCTTCTACC CGCTCGTGTC CGGACGCGAA GACGGCAGCG GCCTCGGCCT CACGCTCGCG CAGACGTTCG TACAGCAGCA CGACGGGATG ATCGAGGTCG AAAGCCGGCC CGGACGTACC GAATTTCAGA TCCTGCTGCC GCTCGACCAT
Details for BuceA.01666.c.A1.GE31827
HARVESTED ON: 3/8/2011
SEQUENCED ON: 3/14/2011
EXPECTED MW: 45kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MVLKNLIKAK TGQPERLTDD ERLSRSGLLA GLEALPTVVI VLDRKTLRIA FANPSAEAML DISRRQLAQR PWGEIFPNAN ELASTITAIG EERFHATHLD TVLDRPGREP LHVHAIVGFL ENAPDFVLVE LFENERQSRT DREERIHDLT AVNKQLIRNL AHEIKNPLGG IRGAAQLLEF ELGERERGEL REYTQVIIKE SDRLQTLVDR LLEPHRHPHI VGDVNIHEVC ERVRAVMLAE FPRGLTIERD YDVSVPDLRG DKEQLIQALL NIVRNAAQAL RERIAQGDAK IELRTRIARK VTIAKRLYRL ALDLHVIDNG PGIPEEIRDR IFYPLVSGRE DGSGLGLTLA QTFVQQHDGM IEVESRPGRT EFQILLPL
Validated NT Sequence
cgagcggcag caggatctga aattcggtac gtccgggccg gctttcgacc tcgatcatcc cgtcgtgctg ctgtacgaac gtctgcgcga gcgtgaggcc gaggccgctg ccgtcttcgc gtccggacac gagcgggtag aagatccggt cgcggatctc ttccggaatg ccgggcccgt tgtcgatcac gtgcaagtcc agtgccagcc ggtacaggcg cttggcgatc gtcaccttgc gcgcgatgcg cgtgcgcagc tcgatcttcg cgtcgccctg cgcgatccgt tcgcgcagcg cctgcgcggc gttgcgcacg atgttgagca gcgcctggat caactgctcc ttgtcgccgc gcaggtccgg cacgctcacg tcgtagtcgc gctcgatcgt gagcccgcgc gggaactccg cgagcatcac cgcgcgcacg cgttcgcaca cttcatgaat gttcacgtcg ccgacgatgt gcggatgccg gtgcggctcc agcaaccggt cgacgagcgt ctgcaggcgg tcggactcct tgatgatcac ctgcgtgtat tcgcgcaact cgccgcgctc gcgctcgccc agctcgaatt cgagcagctg ggctgcgccg cgaatgccgc cgagcgggtt cttgatctcg tgcgcgaggt tgcggatcag ttgcttgttg accgcggtca ggtcgtggat gcgctcttcc cgatcggtgc gcgactgccg ttcgttctcg aacagctcga cgagcacgaa atcgggcgcg ttctcgagga agccgacgat cgcatgcacg tgcagcggtt cgcggccggg ccggtcgagc acggtatcga gatgcgtcgc gtgaaagcgt tcctcgccga tcgcggtgat cgtcgaggcc agctcgttcg cgttcggaaa aatctcgccc cacggccgct gcgcgagctg ccgccgcgag atgtcgagca tcgcctccgc ggacgggttc gcgaacgcga tccgcagcgt cttgcggtcg agcacgatca cgaccgtcgg cagcgcttcg agccccgcca gcagacccga gcgcgagagc cgctcgtcgt ccgtcagtcg ttcgggctgc cccgttttcg ccttgatcag attcttcaga accatcgaac caggacccng ggtctgagct tcc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVLKNLIKA KTGQPERLTD DERLSRSGLL AGLEALPTVV IVLDRKTLRI AFANPSAEAM LDISRRQLAQ RPWGEIFPNA NELASTITAI GEERFHATHL DTVLDRPGRE PLHVHAIVGF LENAPDFVLV ELFENERQSR TDREERIHDL TAVNKQLIRN LAHEIKNPLG GIRGAAQLLE FELGERERGE LREYTQVIIK ESDRLQTLVD RLLEPHRHPH IVGDVNIHEV CERVRAVMLA EFPRGLTIER DYDVSVPDLR GDKEQLIQAL LNIVRNAAQA LRERIAQGDA KIELRTRIAR KVTIAKRLYR LALDLHVIDN GPGIPEEIRD RIFYPLVSGR EDGSGLGLTL AQTFVQQHDG MIEVESRPGR TEFQILLPLD H
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggttctgaag aatctgatca aggcgaaaac ggggcagccc gaacgactga cggacgacga gcggctctcg cgctcgggtc tgctggcggg gctcgaagcg ctgccgacgg tcgtgatcgt gctcgaccgc aagacgctgc ggatcgcgtt cgcgaacccg tccgcggagg cgatgctcga catctcgcgg cggcagctcg cgcagcggcc gtggggcgag atttttccga acgcgaacga gctggcctcg acgatcaccg cgatcggcga ggaacgcttt cacgcgacgc atctcgatac cgtgctcgac cggcccggcc gcgaaccgct gcacgtgcat gcgatcgtcg gcttcctcga gaacgcgccc gatttcgtgc tcgtcgagct gttcgagaac gaacggcagt cgcgcaccga tcgggaagag cgcatccacg acctgaccgc ggtcaacaag caactgatcc gcaacctcgc gcacgagatc aagaacccgc tcggcggcat tcgcggcgca gcccagctgc tcgaattcga gctgggcgag cgcgagcgcg gcgagttgcg cgaatacacg caggtgatca tcaaggagtc cgaccgcctg cagacgctcg tcgaccggtt gctggagccg caccggcatc cgcacatcgt cggcgacgtg aacattcatg aagtgtgcga acgcgtgcgc gcggtgatgc tcgcggagtt cccgcgcggg ctcacgatcg agcgcgacta cgacgtgagc gtgccggacc tgcgcggcga caaggagcag ttgatccagg cgctgctcaa catcgtgcgc aacgccgcgc aggcgctgcg cgaacggatc gcgcagggcg acgcgaagat cgagctgcgc acgcgcatcg cgcgcaaggt gacgatcgcc aagcgcctgt accggctggc actggacttg cacgtgatcg acaacgggcc cggcattccg gaagagatcc gcgaccggat cttctacccg ctcgtgtccg gacgcgaaga cggcagcggc ctcggcctca cgctcgcgca gacgttcgta cagcagcacg acgggatgat cgaggtcgaa agccggcccg gacgtaccga atttcagatc ctgctgccgc tcgaccatta aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a