BuceA.01663.b

Putative haemagglutinin-related autotransporter protein

CENTER ID: BuceA.01663.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01663.b.A2.GE34662 soluble domain 44 864
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01663.b.A2.PS02039 soluble domain 44 864 unavailable

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3913
RefSeq: YP_002232857.1
UniProt: B4EHQ6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNKTYRSIWN AVTNTWTAAA ETAKSHSRGS SRVARQAVIA IALGGAAIGA AAAAEVCTAE DGANGTVDTA GVCQTTTHNL IGPMETTSSI GVMAGGWSGG NGISIWTGTG TAANATNASD IAIGNGASSI AGNGVALGTN ATITSGSAGL AMGAYSTASA SYGIAIGGAT GSGRGAVVEG TGSVAIGTIS HVTSGTSNAV ALGMGSVAHE SNTVSVGAGV PNIDGHTFTR RIVNVSPGIA DTDVVNVSQL APVVTALGGG AAIDPTTGAV TGPTYTLANG GTQTTIGGAL GALDGALTTT NGNVTALTTR MDTAETDISD LQTAVGSGSV GLVQQAGAGA NLTVGKDTDG AAVNFAGTAG NRKLTGVAAG DVSAASTEAV NGSQLHGVSE SVASAIGGGS TVNPDGSISA PSFTVGDGHG GTTTVGTVAD AVSNLDGRTT TNEGAITDLA SQIGSGTLGL VQQANAGDNI TVGASTDGAA VNFAGTAGNR KLTGIAAGDV SAASTEAVNG SQLHGVSESV ASAIGGGSTV NPDGSISAPS FTVGDGSGGT KVVNSVGDAV TNLDGRTTIN EGAITNLAAQ IGSGTLGIVQ QDPASGAITV GAASGGSMVN FAGTGGARVL SGVANGVADD DVVTVSQLRA TGLIDYSGKE VGAVTYDSGL SFDSVTFAGT NGTVLHRVAA GDISATSMDA VNGSQLYALQ QEFAQKYGDL SGRVGDLEAG GGSDGGSGSG VGTGTGGPGS IVVGDGADAS GSNSSAIGQG SVASGNNGSA VGQGAVASGN NGTATGQGAV ASGDNSSAFG QGAVASGGSS TAIGQNANAS GNNSVALGAG SVADRDNAVS VGSAGAERQI TNVAAGTAPT DAVNVQQMNN TVSSARQDAM GGVAAAMAVA GLPQSTQPGR TFVSMAGSTY GGEYGSAMGL SYMTRDGKWT VKAAVNTSSR GEVGGVIGGG FYW
NT Sequence
atgaacaaga cctatcgcag catctggaac gccgtgacca atacatggac ggcggcagcc gaaacggcaa agtcgcattc gagaggttcg tcgcgcgtgg cgcggcaggc tgtgatcgcg atcgcattgg gcggagcggc aatcggcgct gcagccgcag cggaagtgtg tacggcagaa gacggggcga acggtacggt cgatacggct ggcgtgtgtc aaaccacgac tcacaatttg atcggcccga tggaaaccac aagctcgatc ggcgtcatgg ccggcgggtg gagcggcggc aatggaattt cgatctggac gggtaccggt acggcagcca atgccaccaa tgccagcgac atcgcgattg gcaacggagc ctcttcgatc gccggaaacg gtgttgcgct gggaaccaac gcaacaataa ctagcggcag cgccgggctc gcgatgggtg cctattccac cgcttcggca agctacggca ttgccattgg cggcgccaca gggagcggtc gaggtgcggt tgtggaaggc accggttccg tggcaatcgg cacgatttcg catgtcacca gcggtacgag caacgccgtg gctctcggca tgggttccgt tgcacatgaa tccaataccg tctccgtcgg cgcaggggtt cccaacatcg acggtcatac attcacgagg cgaattgtca acgtttcgcc cggtatcgcc gatacggatg tcgtgaacgt ctcgcagctc gcgccggtcg tgaccgcgct cggtggcggc gctgccatcg atccgacgac gggcgccgtt accggcccca cgtacacgct cgccaacggc ggcacgcaaa ccacgattgg cggtgcgctc ggcgccctcg atggcgcgct gacgaccacg aacggcaacg tgactgccct caccacccgc atggataccg ctgaaaccga catcagcgac ctgcagaccg cagtgggctc gggctcggtc ggcctggtgc agcaagccgg tgcaggcgca aacctgaccg tcggcaagga cacggatggc gcggcggtca acttcgccgg cacggccggt aaccgcaagc tgacgggcgt cgcagccggc gacgtgtcgg cagccagcac cgaagccgtg aacggctcgc aactgcacgg tgtgtcggaa agcgtcgcat cggcgatcgg cggcggctcg acggtcaacc cggacggctc gatctcggcg ccgtccttca cggtcggcga cggtcacggc ggcacgacca cggtcggcac ggtcgcggat gcggtctcca acctcgacgg ccgtacgacg accaacgaag gcgcgatcac ggatctcgcc agccagatcg gcagcggcac gctcggcctc gtccagcaag cgaacgccgg cgacaacatc acggtcggcg cgagcaccga cggcgcagcg gtcaacttcg ccggcacggc aggtaaccgt aagctgacag gcatcgcagc cggcgacgtg tcggctgcca gcaccgaagc cgtgaacggc tcgcaactgc atggcgtgtc ggaaagcgtc gcatcggcga tcggcggcgg ctcgacggtc aacccggacg gctcgatctc ggcgccgagc ttcacggtcg gcgacggcag tggcggcacg aaggtcgtca actcggtcgg cgatgccgtg accaacctcg acggtcgtac cacgatcaac gaaggtgcga tcacgaacct cgcggcccag atcggcagcg gcacgctcgg catcgtccag caggacccgg ccagcggcgc gatcacggtc ggcgcggcca gcggtggctc gatggtgaac ttcgccggca ccggcggtgc acgcgtgctg tccggcgtcg ccaacggcgt ggccgacgac gacgtcgtga cggtgtcgca gcttcgtgcg acgggcctga tcgactactc gggcaaggaa gtcggcgcgg tcacgtacga cagcggcttg tcgttcgaca gcgtgacctt cgccggcacg aacggcacgg tcctgcaccg ggttgcggcg ggcgacatca gcgcgacgag catggacgcg gtcaacggca gccagctcta tgccctgcag caggagttcg cgcagaagta cggcgacctg tccggccgcg tcggcgacct cgaggcaggc ggtggctcgg acggtggctc gggcagcggt gtcggcaccg gcacgggcgg ccccggctcg atcgtcgtcg gcgacggcgc ggacgcgtcc ggatcgaaca gctcggccat cggtcagggt tcggtcgcat cgggcaacaa cggttcggcg gtcggtcaag gtgcggttgc ttcgggcaac aacggcacgg caaccggtca gggtgcagtc gcctccggcg acaacagctc ggcattcggc cagggtgcgg tcgcttcggg cggcagcagc acggcaatcg gtcagaacgc gaatgcgtcc ggcaacaact cggtcgcact cggcgcgggc tcggtcgcgg atcgcgataa cgcagtgtct gtcggctccg ccggtgccga acgccagatc acgaacgtcg cggccggtac ggcgccgacc gacgcggtca acgtgcagca gatgaacaac acggtcagca gcgcgcgtca ggacgcaatg ggcggcgttg ccgcggcgat ggcggtggca ggcctgccgc agtcgacgca gccgggccgc acgttcgtgt cgatggccgg cagcacgtac ggcggcgagt acggctcggc gatgggtctg tcgtacatga cccgcgacgg caagtggacg gtcaaggctg cggtcaacac gagcagccgc ggcgaagtcg gcggcgtgat cggcggcggg ttctactggt aa
Details for BuceA.01663.b.A2.GE34662
HARVESTED ON: 1/31/2012
SEQUENCED ON: 3/16/2012
EXPECTED MW: 78kDa
OBSERVED MW: 90kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 22
PERCENT COVERAGE: 40
Validated AA Sequence
MTVDVGNPCA DGDGIGFMCN GTHAESHGVA RTAGDMRNRA DCHGTGAFHN RTSTAPCGAA NGNAVACRSG GIGTHREPGA AASYCCVGSQ RNTVSGDRRG SVANRDVAGI GGIGCRTGTR PDRNSIAAAP PAGHDADRAC GFHRADQIVS RGLTHASRID RTVRPVFCRT HFRCGCSADC RAAAARSDRT LAECRAVVAG GDCTLTGCRA VVARSNRTLT DRRTVVARCD RTLTDGRAVR SXRVRXXXXX XXXGXXXXGA XXXAXXTVXX XXXXXVXXXX XQVAXLXXEL LLXXIXLXXV DXXXXXXXXX XXXXXXXXXA VRXXXXXXXX XXXXVXXXXX XXXX
Validated NT Sequence
cggncannga ngtnnnnnnn nnnnngtnnn acagnnnnnn gtnnnnngnc annnnnacnn nnncnnncac gaacggcang ntcnnnnnnn nnnnnnnnnn nnnnnnnatn annnnnnnnn ncanngannn ggtcaacnnc anccagnnnt atnncctnca gcaggagttc gngcanaagt anggcgacct gtcnnnnngn ntnnnngacc tnnnnncann nnnnnnntnn nacggtggnt nnngcagnnn nntnggcacc gncacnnnnn nccccggntn natnntnntn ngngncnnng cggacgcgtc nggatcgaac agctcgncca tcggtcaggg ttcggtcgca tcgggcaaca acggttcggc ggtcggtcaa ggtgcggttg cttcgggcaa caacggcacg gcaaccggtc agggtgcagt cgcctccggc gacaacagct cggcattcgg ccagggtgcg gtcgcttcgg gcggcagcag cacggcaatc ggcgctgcag ccgcagcgga agtgtgtacg gcagaagacg gggcgaacgg tacggtcgat acggctggcg tgtgtcaaac cacgactcac aatttgatcg gcccgatgga aaccacaagc tcgatcggcg tcatggccgg cgggtggagc ggcggcaatg gaatttcgat ctggacgggt accggtacgg cagccaatgc caccaatgcc agcgacatcg cgattggcaa cggagcctct tcgatcgccg gaaacggtgt tgcgctggga accaacgcaa caataactag cggcagcgcc gggctcgcga tgggtgccta ttccaccgct tcggcaagct acggcattgc cattggcggc gccacaggga gcggtcgagg tgcggttgtg gaaggcaccg gttccgtggc aatcggcacg atttcgcatg tcaccagcgg tacgagcaac gccgtggctc tcggcatggg ttccgttgca catgaatcca ataccgtctc cgtcggcgca ggggttccca acatcgacgg tcatacattc acgaggcgaa ttgtcaacgt ttcgcccggt atcgccgata cggatgtcgt gaacgtctcg cagctcgcgc cggtcgtgac cgcgctcggt ggcggcgctg ccatcgatcc gacgacgggc gccgttaccg gccccacgta cacgctcgcc aacngcggca cgcaaaccac gattggcggt gcgctcngcg ccctcnatgg cgcgctgac
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGGAAIGAA AAAEVCTAED GANGTVDTAG VCQTTTHNLI GPMETTSSIG VMAGGWSGGN GISIWTGTGT AANATNASDI AIGNGASSIA GNGVALGTNA TITSGSAGLA MGAYSTASAS YGIAIGGATG SGRGAVVEGT GSVAIGTISH VTSGTSNAVA LGMGSVAHES NTVSVGAGVP NIDGHTFTRR IVNVSPGIAD TDVVNVSQLA PVVTALGGGA AIDPTTGAVT GPTYTLANGG TQTTIGGALG ALDGALTTTN GNVTALTTRM DTAETDISDL QTAVGSGSVG LVQQAGAGAN LTVGKDTDGA AVNFAGTAGN RKLTGVAAGD VSAASTEAVN GSQLHGVSES VASAIGGGST VNPDGSISAP SFTVGDGHGG TTTVGTVADA VSNLDGRTTT NEGAITDLAS QIGSGTLGLV QQANAGDNIT VGASTDGAAV NFAGTAGNRK LTGIAAGDVS AASTEAVNGS QLHGVSESVA SAIGGGSTVN PDGSISAPSF TVGDGSGGTK VVNSVGDAVT NLDGRTTINE GAITNLAAQI GSGTLGIVQQ DPASGAITVG AASGGSMVNF AGTGGARVLS GVANGVADDD VVTVSQLRAT GLIDYSGKEV GAVTYDSGLS FDSVTFAGTN GTVLHRVAAG DISATSMDAV NGSQLYALQQ EFAQKYGDLS GRVGDLEAGG GSDGGSGSGV GTGTGGPGSI VVGDGADASG SNSSAIGQGS VASGNNGSAV GQGAVASGNN GTATGQGAVA SGDNSSAFGQ GAVASGGSST AIGQNANASG NNSVALGAGS VADRDNAVSV GSAGAERQIT NVAAGTAPTD AVN
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gggcggagcg gcaatcggcg ctgcagccgc agcggaagtg tgtacggcag aagacggggc gaacggtacg gtcgatacgg ctggcgtgtg tcaaaccacg actcacaatt tgatcggccc gatggaaacc acaagctcga tcggcgtcat ggccggcggg tggagcggcg gcaatggaat ttcgatctgg acgggtaccg gtacggcagc caatgccacc aatgccagcg acatcgcgat tggcaacgga gcctcttcga tcgccggaaa cggtgttgcg ctgggaacca acgcaacaat aactagcggc agcgccgggc tcgcgatggg tgcctattcc accgcttcgg caagctacgg cattgccatt ggcggcgcca cagggagcgg tcgaggtgcg gttgtggaag gcaccggttc cgtggcaatc ggcacgattt cgcatgtcac cagcggtacg agcaacgccg tggctctcgg catgggttcc gttgcacatg aatccaatac cgtctccgtc ggcgcagggg ttcccaacat cgacggtcat acattcacga ggcgaattgt caacgtttcg cccggtatcg ccgatacgga tgtcgtgaac gtctcgcagc tcgcgccggt cgtgaccgcg ctcggtggcg gcgctgccat cgatccgacg acgggcgccg ttaccggccc cacgtacacg ctcgccaacg gcggcacgca aaccacgatt ggcggtgcgc tcggcgccct cgatggcgcg ctgacgacca cgaacggcaa cgtgactgcc ctcaccaccc gcatggatac cgctgaaacc gacatcagcg acctgcagac cgcagtgggc tcgggctcgg tcggcctggt gcagcaagcc ggtgcaggcg caaacctgac cgtcggcaag gacacggatg gcgcggcggt caacttcgcc ggcacggccg gtaaccgcaa gctgacgggc gtcgcagccg gcgacgtgtc ggcagccagc accgaagccg tgaacggctc gcaactgcac ggtgtgtcgg aaagcgtcgc atcggcgatc ggcggcggct cgacggtcaa cccggacggc tcgatctcgg cgccgtcctt cacggtcggc gacggtcacg gcggcacgac cacggtcggc acggtcgcgg atgcggtctc caacctcgac ggccgtacga cgaccaacga aggcgcgatc acggatctcg ccagccagat cggcagcggc acgctcggcc tcgtccagca agcgaacgcc ggcgacaaca tcacggtcgg cgcgagcacc gacggcgcag cggtcaactt cgccggcacg gcaggtaacc gtaagctgac aggcatcgca gccggcgacg tgtcggctgc cagcaccgaa gccgtgaacg gctcgcaact gcatggcgtg tcggaaagcg tcgcatcggc gatcggcggc ggctcgacgg tcaacccgga cggctcgatc tcggcgccga gcttcacggt cggcgacggc agtggcggca cgaaggtcgt caactcggtc ggcgatgccg tgaccaacct cgacggtcgt accacgatca acgaaggtgc gatcacgaac ctcgcggccc agatcggcag cggcacgctc ggcatcgtcc agcaggaccc ggccagcggc gcgatcacgg tcggcgcggc cagcggtggc tcgatggtga acttcgccgg caccggcggt gcacgcgtgc tgtccggcgt cgccaacggc gtggccgacg acgacgtcgt gacggtgtcg cagcttcgtg cgacgggcct gatcgactac tcgggcaagg aagtcggcgc ggtcacgtac gacagcggct tgtcgttcga cagcgtgacc ttcgccggca cgaacggcac ggtcctgcac cgggttgcgg cgggcgacat cagcgcgacg agcatggacg cggtcaacgg cagccagctc tatgccctgc agcaggagtt cgcgcagaag tacggcgacc tgtccggccg cgtcggcgac ctcgaggcag gcggtggctc ggacggtggc tcgggcagcg gtgtcggcac cggcacgggc ggccccggct cgatcgtcgt cggcgacggc gcggacgcgt ccggatcgaa cagctcggcc atcggtcagg gttcggtcgc atcgggcaac aacggttcgg cggtcggtca aggtgcggtt gcttcgggca acaacggcac ggcaaccggt cagggtgcag tcgcctccgg cgacaacagc tcggcattcg gccagggtgc ggtcgcttcg ggcggcagca gcacggcaat cggtcagaac gcgaatgcgt ccggcaacaa ctcggtcgca ctcggcgcgg gctcggtcgc ggatcgcgat aacgcagtgt ctgtcggctc cgccggtgcc gaacgccaga tcacgaacgt cgcggccggt acggcgccga ccgacgcggt caactaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa
Details for BuceA.01663.b.A2.PS02039
PURIFICATION DATe: 4/10/2014
CONCENTRATION: 18.66mg/ml
OBSERVED MW: 90kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): data unavailable
VIAL VOLUME: 110µl
PERCENT IDENTITY: 22
PERCENT COVERAGE: 40
Protocol Notes
notes unavailable
Validated AA Sequence
MTVDVGNPCA DGDGIGFMCN GTHAESHGVA RTAGDMRNRA DCHGTGAFHN RTSTAPCGAA NGNAVACRSG GIGTHREPGA AASYCCVGSQ RNTVSGDRRG SVANRDVAGI GGIGCRTGTR PDRNSIAAAP PAGHDADRAC GFHRADQIVS RGLTHASRID RTVRPVFCRT HFRCGCSADC RAAAARSDRT LAECRAVVAG GDCTLTGCRA VVARSNRTLT DRRTVVARCD RTLTDGRAVR SXRVRXXXXX XXXGXXXXGA XXXAXXTVXX XXXXXVXXXX XQVAXLXXEL LLXXIXLXXV DXXXXXXXXX XXXXXXXXXA VRXXXXXXXX XXXXVXXXXX XXXX
Validated NT Sequence
cggncannga ngtnnnnnnn nnnnngtnnn acagnnnnnn gtnnnnngnc annnnnacnn nnncnnncac gaacggcang ntcnnnnnnn nnnnnnnnnn nnnnnnnatn annnnnnnnn ncanngannn ggtcaacnnc anccagnnnt atnncctnca gcaggagttc gngcanaagt anggcgacct gtcnnnnngn ntnnnngacc tnnnnncann nnnnnnntnn nacggtggnt nnngcagnnn nntnggcacc gncacnnnnn nccccggntn natnntnntn ngngncnnng cggacgcgtc nggatcgaac agctcgncca tcggtcaggg ttcggtcgca tcgggcaaca acggttcggc ggtcggtcaa ggtgcggttg cttcgggcaa caacggcacg gcaaccggtc agggtgcagt cgcctccggc gacaacagct cggcattcgg ccagggtgcg gtcgcttcgg gcggcagcag cacggcaatc ggcgctgcag ccgcagcgga agtgtgtacg gcagaagacg gggcgaacgg tacggtcgat acggctggcg tgtgtcaaac cacgactcac aatttgatcg gcccgatgga aaccacaagc tcgatcggcg tcatggccgg cgggtggagc ggcggcaatg gaatttcgat ctggacgggt accggtacgg cagccaatgc caccaatgcc agcgacatcg cgattggcaa cggagcctct tcgatcgccg gaaacggtgt tgcgctggga accaacgcaa caataactag cggcagcgcc gggctcgcga tgggtgccta ttccaccgct tcggcaagct acggcattgc cattggcggc gccacaggga gcggtcgagg tgcggttgtg gaaggcaccg gttccgtggc aatcggcacg atttcgcatg tcaccagcgg tacgagcaac gccgtggctc tcggcatggg ttccgttgca catgaatcca ataccgtctc cgtcggcgca ggggttccca acatcgacgg tcatacattc acgaggcgaa ttgtcaacgt ttcgcccggt atcgccgata cggatgtcgt gaacgtctcg cagctcgcgc cggtcgtgac cgcgctcggt ggcggcgctg ccatcgatcc gacgacgggc gccgttaccg gccccacgta cacgctcgcc aacngcggca cgcaaaccac gattggcggt gcgctcngcg ccctcnatgg cgcgctgac
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMGGAAIGAA AAAEVCTAED GANGTVDTAG VCQTTTHNLI GPMETTSSIG VMAGGWSGGN GISIWTGTGT AANATNASDI AIGNGASSIA GNGVALGTNA TITSGSAGLA MGAYSTASAS YGIAIGGATG SGRGAVVEGT GSVAIGTISH VTSGTSNAVA LGMGSVAHES NTVSVGAGVP NIDGHTFTRR IVNVSPGIAD TDVVNVSQLA PVVTALGGGA AIDPTTGAVT GPTYTLANGG TQTTIGGALG ALDGALTTTN GNVTALTTRM DTAETDISDL QTAVGSGSVG LVQQAGAGAN LTVGKDTDGA AVNFAGTAGN RKLTGVAAGD VSAASTEAVN GSQLHGVSES VASAIGGGST VNPDGSISAP SFTVGDGHGG TTTVGTVADA VSNLDGRTTT NEGAITDLAS QIGSGTLGLV QQANAGDNIT VGASTDGAAV NFAGTAGNRK LTGIAAGDVS AASTEAVNGS QLHGVSESVA SAIGGGSTVN PDGSISAPSF TVGDGSGGTK VVNSVGDAVT NLDGRTTINE GAITNLAAQI GSGTLGIVQQ DPASGAITVG AASGGSMVNF AGTGGARVLS GVANGVADDD VVTVSQLRAT GLIDYSGKEV GAVTYDSGLS FDSVTFAGTN GTVLHRVAAG DISATSMDAV NGSQLYALQQ EFAQKYGDLS GRVGDLEAGG GSDGGSGSGV GTGTGGPGSI VVGDGADASG SNSSAIGQGS VASGNNGSAV GQGAVASGNN GTATGQGAVA SGDNSSAFGQ GAVASGGSST AIGQNANASG NNSVALGAGS VADRDNAVSV GSAGAERQIT NVAAGTAPTD AVN
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GGGCGGAGCG GCAATCGGCG CTGCAGCCGC AGCGGAAGTG TGTACGGCAG AAGACGGGGC GAACGGTACG GTCGATACGG CTGGCGTGTG TCAAACCACG ACTCACAATT TGATCGGCCC GATGGAAACC ACAAGCTCGA TCGGCGTCAT GGCCGGCGGG TGGAGCGGCG GCAATGGAAT TTCGATCTGG ACGGGTACCG GTACGGCAGC CAATGCCACC AATGCCAGCG ACATCGCGAT TGGCAACGGA GCCTCTTCGA TCGCCGGAAA CGGTGTTGCG CTGGGAACCA ACGCAACAAT AACTAGCGGC AGCGCCGGGC TCGCGATGGG TGCCTATTCC ACCGCTTCGG CAAGCTACGG CATTGCCATT GGCGGCGCCA CAGGGAGCGG TCGAGGTGCG GTTGTGGAAG GCACCGGTTC CGTGGCAATC GGCACGATTT CGCATGTCAC CAGCGGTACG AGCAACGCCG TGGCTCTCGG CATGGGTTCC GTTGCACATG AATCCAATAC CGTCTCCGTC GGCGCAGGGG TTCCCAACAT CGACGGTCAT ACATTCACGA GGCGAATTGT CAACGTTTCG CCCGGTATCG CCGATACGGA TGTCGTGAAC GTCTCGCAGC TCGCGCCGGT CGTGACCGCG CTCGGTGGCG GCGCTGCCAT CGATCCGACG ACGGGCGCCG TTACCGGCCC CACGTACACG CTCGCCAACG GCGGCACGCA AACCACGATT GGCGGTGCGC TCGGCGCCCT CGATGGCGCG CTGACGACCA CGAACGGCAA CGTGACTGCC CTCACCACCC GCATGGATAC CGCTGAAACC GACATCAGCG ACCTGCAGAC CGCAGTGGGC TCGGGCTCGG TCGGCCTGGT GCAGCAAGCC GGTGCAGGCG CAAACCTGAC CGTCGGCAAG GACACGGATG GCGCGGCGGT CAACTTCGCC GGCACGGCCG GTAACCGCAA GCTGACGGGC GTCGCAGCCG GCGACGTGTC GGCAGCCAGC ACCGAAGCCG TGAACGGCTC GCAACTGCAC GGTGTGTCGG AAAGCGTCGC ATCGGCGATC GGCGGCGGCT CGACGGTCAA CCCGGACGGC TCGATCTCGG CGCCGTCCTT CACGGTCGGC GACGGTCACG GCGGCACGAC CACGGTCGGC ACGGTCGCGG ATGCGGTCTC CAACCTCGAC GGCCGTACGA CGACCAACGA AGGCGCGATC ACGGATCTCG CCAGCCAGAT CGGCAGCGGC ACGCTCGGCC TCGTCCAGCA AGCGAACGCC GGCGACAACA TCACGGTCGG CGCGAGCACC GACGGCGCAG CGGTCAACTT CGCCGGCACG GCAGGTAACC GTAAGCTGAC AGGCATCGCA GCCGGCGACG TGTCGGCTGC CAGCACCGAA GCCGTGAACG GCTCGCAACT GCATGGCGTG TCGGAAAGCG TCGCATCGGC GATCGGCGGC GGCTCGACGG TCAACCCGGA CGGCTCGATC TCGGCGCCGA GCTTCACGGT CGGCGACGGC AGTGGCGGCA CGAAGGTCGT CAACTCGGTC GGCGATGCCG TGACCAACCT CGACGGTCGT ACCACGATCA ACGAAGGTGC GATCACGAAC CTCGCGGCCC AGATCGGCAG CGGCACGCTC GGCATCGTCC AGCAGGACCC GGCCAGCGGC GCGATCACGG TCGGCGCGGC CAGCGGTGGC TCGATGGTGA ACTTCGCCGG CACCGGCGGT GCACGCGTGC TGTCCGGCGT CGCCAACGGC GTGGCCGACG ACGACGTCGT GACGGTGTCG CAGCTTCGTG CGACGGGCCT GATCGACTAC TCGGGCAAGG AAGTCGGCGC GGTCACGTAC GACAGCGGCT TGTCGTTCGA CAGCGTGACC TTCGCCGGCA CGAACGGCAC GGTCCTGCAC CGGGTTGCGG CGGGCGACAT CAGCGCGACG AGCATGGACG CGGTCAACGG CAGCCAGCTC TATGCCCTGC AGCAGGAGTT CGCGCAGAAG TACGGCGACC TGTCCGGCCG CGTCGGCGAC CTCGAGGCAG GCGGTGGCTC GGACGGTGGC TCGGGCAGCG GTGTCGGCAC CGGCACGGGC GGCCCCGGCT CGATCGTCGT CGGCGACGGC GCGGACGCGT CCGGATCGAA CAGCTCGGCC ATCGGTCAGG GTTCGGTCGC ATCGGGCAAC AACGGTTCGG CGGTCGGTCA AGGTGCGGTT GCTTCGGGCA ACAACGGCAC GGCAACCGGT CAGGGTGCAG TCGCCTCCGG CGACAACAGC TCGGCATTCG GCCAGGGTGC GGTCGCTTCG GGCGGCAGCA GCACGGCAAT CGGTCAGAAC GCGAATGCGT CCGGCAACAA CTCGGTCGCA CTCGGCGCGG GCTCGGTCGC GGATCGCGAT AACGCAGTGT CTGTCGGCTC CGCCGGTGCC GAACGCCAGA TCACGAACGT CGCGGCCGGT ACGGCGCCGA CCGACGCGGT CAACTAAACA GCACGAACAA GTTCTGCAGC CAAGCTTCTC GAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAA