SSGCID: Target Page

BuceA.01643.a

UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-dia minopimelate ligase (EC 6.3.2.13)

CENTER ID:	BuceA.01643.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01643.a.B1.GE35038	full length		1	512

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01643.a.B1.PW35653	full length		1	512

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.3601
RefSeq:	YP_002232569.1
UniProt:	B4E6J7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSAARSSHPA HQQIAAALAW LRQHVAPAAQ LHADTRSLQA GDVFVAYAVD GADNRAFMAD AVARGAAAVL YQPDGLTDAP AVPVALAVPA LDQLAGEIAS GWYGDPSDSL LAVGVTGTNG KTSCTQWIAT ALTALHQPCA VIGTLGTGMP GQLVPTGFTT PDAPQLQRSL AQLRAAGAQA VAMEVSSHAL HQGRVNGTAF DIAVFTNLTQ DHLDYHGTFD AYEAAKAKLF AWRGLRAAVL NRDDAAGRRL LEKLAGRVRT IAYGIGDAPA PDADRELVAL DVRATATGTA FRLRSSWGDA DVEVGTLGTF NVSNLLAVLG SLLAADVPFD AALAEIERLE SVNGRMQRLG GRLQNDEPLV VIDYAHTPDA LDKTLDALRP IAAARGGRLV CMFGCGGDRD ATKRPLMGAI AERLADEVVV TSDNPRSEDP QHIIDQVIAG MTAPERARRI EDRASAILQA VRGAAREDVV VLAGKGHEAT QEIMGKKRTF SDQDHARLAL AARATHGKGG AE

NT Sequence

atgagcgccg cccgcagttc ccatccggcg catcagcaga tcgcagccgc gctcgcgtgg ctgcgtcagc atgtggcccc cgcggcgcaa ctgcatgccg acacgcgcag cctccaggct ggcgacgtgt tcgtcgcgta tgcggtcgac ggggcggata accgcgcttt catggccgac gccgtcgcgc gcggtgcggc cgccgtgctg tatcagccgg acgggctgac cgatgcgccg gccgtccccg tcgcgctcgc ggtgccggcg ctcgaccagc tcgcgggcga gatcgccagc ggctggtacg gcgatccgag cgacagcctg ctcgcggtcg gtgtcacggg tacgaacggc aagacgtcgt gcacgcagtg gatcgccacc gcgctgacgg cgctgcatca gccgtgcgcg gtgatcggca cgctcggcac cgggatgccc ggccagctcg tgccgaccgg cttcacgacg cccgacgcgc cgcaactgca gcgcagcctc gcgcaattgc gtgcggcggg cgcgcaggcc gtggcgatgg aagtgtcgtc gcatgcgctg caccaggggc gcgtgaacgg gaccgcgttc gacatcgcgg tgtttacgaa cctcacgcag gatcacctcg actatcacgg cacgttcgac gcgtacgagg ccgcgaaggc gaagctgttc gcgtggcgcg gtctgcgcgc ggcggtgctc aaccgcgacg atgcggctgg ccgccgcctg ctcgagaagc tggccggccg cgtgcgcacg atcgcgtacg ggatcggcga cgcaccggcg cccgacgccg atcgcgaact ggtcgcgctc gacgtgcgcg ccaccgcgac gggtaccgcc ttccgcctgc gttcgtcgtg gggcgacgcg gacgtcgagg ttggcacgct cggcacgttc aacgtcagca atctgctcgc cgtgctcggc tcgctgctcg cggccgacgt gccgttcgac gcggccctcg ccgagatcga gcggctcgag tcggtcaatg gccggatgca gcggctcggc ggccggctgc agaacgacga accgctcgtc gtgatcgatt acgcacatac gcccgacgcg ctcgacaaga cgctcgacgc gctgcgcccg atcgccgcgg cgcgcggcgg ccggctcgtc tgcatgttcg gctgcggcgg cgatcgcgat gcgacgaagc gcccgctgat gggcgcgatc gcggagcggc tcgccgacga agtcgtcgtc acgagcgaca acccgcgcag cgaagatccg cagcacatca tcgaccaggt catcgcgggc atgaccgcgc ccgagcgtgc acgccgtatc gaggatcgcg cgagcgcgat cctgcaggcg gtgcgcggcg ccgcacgcga ggatgtcgtc gtgctggccg gcaagggcca cgaggctacg caggaaatca tgggcaagaa gcgcaccttc tccgaccagg atcacgcgcg gctcgcgctt gcagcgcgcg ccacgcacgg caagggaggc gccgaatga

Details for BuceA.01643.a.B1.GE35038

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	54kDa
OBSERVED MW:	54kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	99
PERCENT COVERAGE:	85

Validated AA Sequence

MADAVARGAA AVLYQPDGLT DAPAVPVALA VPALDQLAGE IASGWYGDPS DSLLAVGVTG TNGXTSCTQW IATALTALHQ PCAVIGTLGT GXPGQLVPTG FTTPDAPQLQ RSLAQLRAAG AQAVAMEVSS HALHQGRVNG TAFDIAVFTN LTXDHLDYHG TFDAYEAAKA KLFAWRGLRA AVLNRDDAAG RRLLEKLAGR VRTIAYGIGD APAPDADREL VALDVRATAT GTAFRLRSSW GDADVEVGTL GTFNVSNLLA VLGSLLAADV PFDAALAEIE RLESVNGRMQ RLGGRLQNDE PLVVIDYAHT PDALDKTLDA LRPIAAARGG RLVCMFGCGG DRDATKRPLM GAIAERLADE VVVTSDNPRS EDPQHIIDQV IAGMTAPERA RRIEDRASAI LQAVRGAARE DVVVLAGKGH EATQEIMGKK RTFSDQDHAR LALA

Validated NT Sequence

cgctgcaagc gcgagccgcg cgtgatcctg gtcggagaag gtgcgcttct tgcccatgat ttcctgcgta gcctcgtggc ccttgccggc cagcacgacg acatcctcgc gtgcggcgcc gcgcaccgcc tgcaggatcg cgctcgcgcg atcctcgata cggcgtgcac gctcgggcgc ggtcatgccc gcgatgacct ggtcgatgat gtgctgcgga tcttcgctgc gcgggttgtc gctcgtgacg acgacttcgt cggcgagccg ctccgcgatc gcgcccatca gcgggcgctt cgtcgcatcg cgatcgccgc cgcagccgaa catgcagacg agccggccgc cgcgcgccgc ggcgatcggg cgcagcgcgt cgagcgtctt gtcgagcgcg tcgggcgtat gtgcgtaatc gatcacgacg agcggttcgt cgttctgcag ccggccgccg agccgctgca tccggccatt gaccgactcg agccgctcga tctcggcgag ggccgcgtcg aacggcacgt cggccgcgag cagcgagccg agcacggcga gcagattgct gacgttgaac gtgccgagcg tgccaacctc gacgtccgcg tcgccccacg acgaacgcag gcggaaggcg gtacccgtcg cggtggcgcg cacgtcgagc gcgaccagtt cgcgatcggc gtcgggcgcc ggtgcgtcgc cgatcccgta cgcgatcgtg cgcacgcggc cggccagctt ctcgagcagg cggcggccag ccgcatcgtc gcggttgagc accgccgcgc gcagaccgcg ccacgcgaac agcttcgcct tcgcggcctc gtacgcgtcg aacgtgccgt gatagtcgag gtgatcnngc gtgaggttcg taaacaccgc gatgtcgaac gcggtcccgt tcacgcgccc ctggtgcagc gcatgcgacg acacttccat cgccacggcc tgcgcgcccg ccgcacgcaa ttgcgcgagg ctgcgctgca gttgcggcgc gtcgggcgtc gtgaagccgg tcggcacgag ctggccgggc ancccggtgc cgagcgtgcc gatcaccgcg cacggctgat gcagcgccgt cagcgcggtg gcgatccact gcgtgcacga cgtctngccg ttcgtacccg tgacaccgac cgcgagcagg ctgtcgctcg gatcgccgta ccagccgctg gcgatctcgc ccgcgagctg gtcgagcgcc ggcaccgcga gcgcgacggg gacggccggc gcatcggtca gcccgtccgg ctgatacagc acggcggccg caccgcgcgc gacggcgtcg gccatgaaag cgcggttatc cgccccgtcg accgcatacg cgangaacac gtcgccagcc tggaggntgc gcgtgtcggc atgcagttgc gccgcggggg ccacatgctg acgcagccac gcgagcgcgg ctgcgatnng ntgatgcgcc ggatgggaac tgcgggcggc gctc

Expected Protein Sequence

MAHHHHHHMS AARSSHPAHQ QIAAALAWLR QHVAPAAQLH ADTRSLQAGD VFVAYAVDGA DNRAFMADAV ARGAAAVLYQ PDGLTDAPAV PVALAVPALD QLAGEIASGW YGDPSDSLLA VGVTGTNGKT SCTQWIATAL TALHQPCAVI GTLGTGMPGQ LVPTGFTTPD APQLQRSLAQ LRAAGAQAVA MEVSSHALHQ GRVNGTAFDI AVFTNLTQDH LDYHGTFDAY EAAKAKLFAW RGLRAAVLNR DDAAGRRLLE KLAGRVRTIA YGIGDAPAPD ADRELVALDV RATATGTAFR LRSSWGDADV EVGTLGTFNV SNLLAVLGSL LAADVPFDAA LAEIERLESV NGRMQRLGGR LQNDEPLVVI DYAHTPDALD KTLDALRPIA AARGGRLVCM FGCGGDRDAT KRPLMGAIAE RLADEVVVTS DNPRSEDPQH IIDQVIAGMT APERARRIED RASAILQAVR GAAREDVVVL AGKGHEATQE IMGKKRTFSD QDHARLALAA RATHGKGGAE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcg ccgcccgcag ttcccatccg gcgcatcagc agatcgcagc cgcgctcgcg tggctgcgtc agcatgtggc ccccgcggcg caactgcatg ccgacacgcg cagcctccag gctggcgacg tgttcgtcgc gtatgcggtc gacggggcgg ataaccgcgc tttcatggcc gacgccgtcg cgcgcggtgc ggccgccgtg ctgtatcagc cggacgggct gaccgatgcg ccggccgtcc ccgtcgcgct cgcggtgccg gcgctcgacc agctcgcggg cgagatcgcc agcggctggt acggcgatcc gagcgacagc ctgctcgcgg tcggtgtcac gggtacgaac ggcaagacgt cgtgcacgca gtggatcgcc accgcgctga cggcgctgca tcagccgtgc gcggtgatcg gcacgctcgg caccgggatg cccggccagc tcgtgccgac cggcttcacg acgcccgacg cgccgcaact gcagcgcagc ctcgcgcaat tgcgtgcggc gggcgcgcag gccgtggcga tggaagtgtc gtcgcatgcg ctgcaccagg ggcgcgtgaa cgggaccgcg ttcgacatcg cggtgtttac gaacctcacg caggatcacc tcgactatca cggcacgttc gacgcgtacg aggccgcgaa ggcgaagctg ttcgcgtggc gcggtctgcg cgcggcggtg ctcaaccgcg acgatgcggc tggccgccgc ctgctcgaga agctggccgg ccgcgtgcgc acgatcgcgt acgggatcgg cgacgcaccg gcgcccgacg ccgatcgcga actggtcgcg ctcgacgtgc gcgccaccgc gacgggtacc gccttccgcc tgcgttcgtc gtggggcgac gcggacgtcg aggttggcac gctcggcacg ttcaacgtca gcaatctgct cgccgtgctc ggctcgctgc tcgcggccga cgtgccgttc gacgcggccc tcgccgagat cgagcggctc gagtcggtca atggccggat gcagcggctc ggcggccggc tgcagaacga cgaaccgctc gtcgtgatcg attacgcaca tacgcccgac gcgctcgaca agacgctcga cgcgctgcgc ccgatcgccg cggcgcgcgg cggccggctc gtctgcatgt tcggctgcgg cggcgatcgc gatgcgacga agcgcccgct gatgggcgcg atcgcggagc ggctcgccga cgaagtcgtc gtcacgagcg acaacccgcg cagcgaagat ccgcagcaca tcatcgacca ggtcatcgcg ggcatgaccg cgcccgagcg tgcacgccgt atcgaggatc gcgcgagcgc gatcctgcag gcggtgcgcg gcgccgcacg cgaggatgtc gtcgtgctgg ccggcaaggg ccacgaggct acgcaggaaa tcatgggcaa gaagcgcacc ttctccgacc aggatcacgc gcggctcgcg cttgcagcgc gcgccacgca cggcaaggga ggcgccgaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BuceA.01643.a.B1.PW35653

PURIFICATION DATe:	4/24/2012
CONCENTRATION:	39.06mg/ml
OBSERVED MW:	56kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	15
VIAL VOLUME:	200µl
PERCENT IDENTITY:	99
PERCENT COVERAGE:	85

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCG CCGCCCGCAG TTCCCATCCG GCGCATCAGC AGATCGCAGC CGCGCTCGCG TGGCTGCGTC AGCATGTGGC CCCCGCGGCG CAACTGCATG CCGACACGCG CAGCCTCCAG GCTGGCGACG TGTTCGTCGC GTATGCGGTC GACGGGGCGG ATAACCGCGC TTTCATGGCC GACGCCGTCG CGCGCGGTGC GGCCGCCGTG CTGTATCAGC CGGACGGGCT GACCGATGCG CCGGCCGTCC CCGTCGCGCT CGCGGTGCCG GCGCTCGACC AGCTCGCGGG CGAGATCGCC AGCGGCTGGT ACGGCGATCC GAGCGACAGC CTGCTCGCGG TCGGTGTCAC GGGTACGAAC GGCAAGACGT CGTGCACGCA GTGGATCGCC ACCGCGCTGA CGGCGCTGCA TCAGCCGTGC GCGGTGATCG GCACGCTCGG CACCGGGATG CCCGGCCAGC TCGTGCCGAC CGGCTTCACG ACGCCCGACG CGCCGCAACT GCAGCGCAGC CTCGCGCAAT TGCGTGCGGC GGGCGCGCAG GCCGTGGCGA TGGAAGTGTC GTCGCATGCG CTGCACCAGG GGCGCGTGAA CGGGACCGCG TTCGACATCG CGGTGTTTAC GAACCTCACG CAGGATCACC TCGACTATCA CGGCACGTTC GACGCGTACG AGGCCGCGAA GGCGAAGCTG TTCGCGTGGC GCGGTCTGCG CGCGGCGGTG CTCAACCGCG ACGATGCGGC TGGCCGCCGC CTGCTCGAGA AGCTGGCCGG CCGCGTGCGC ACGATCGCGT ACGGGATCGG CGACGCACCG GCGCCCGACG CCGATCGCGA ACTGGTCGCG CTCGACGTGC GCGCCACCGC GACGGGTACC GCCTTCCGCC TGCGTTCGTC GTGGGGCGAC GCGGACGTCG AGGTTGGCAC GCTCGGCACG TTCAACGTCA GCAATCTGCT CGCCGTGCTC GGCTCGCTGC TCGCGGCCGA CGTGCCGTTC GACGCGGCCC TCGCCGAGAT CGAGCGGCTC GAGTCGGTCA ATGGCCGGAT GCAGCGGCTC GGCGGCCGGC TGCAGAACGA CGAACCGCTC GTCGTGATCG ATTACGCACA TACGCCCGAC GCGCTCGACA AGACGCTCGA CGCGCTGCGC CCGATCGCCG CGGCGCGCGG CGGCCGGCTC GTCTGCATGT TCGGCTGCGG CGGCGATCGC GATGCGACGA AGCGCCCGCT GATGGGCGCG ATCGCGGAGC GGCTCGCCGA CGAAGTCGTC GTCACGAGCG ACAACCCGCG CAGCGAAGAT CCGCAGCACA TCATCGACCA GGTCATCGCG GGCATGACCG CGCCCGAGCG TGCACGCCGT ATCGAGGATC GCGCGAGCGC GATCCTGCAG GCGGTGCGCG GCGCCGCACG CGAGGATGTC GTCGTGCTGG CCGGCAAGGG CCACGAGGCT ACGCAGGAAA TCATGGGCAA GAAGCGCACC TTCTCCGACC AGGATCACGC GCGGCTCGCG CTTGCAGCGC GCGCCACGCA CGGCAAGGGA GGCGCCGAAT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT