BuceA.01545.a

Ribosomal RNA small subunit methyltransferase H (EC 2.1.1.199) (16S rRNA m(4)C1402 methyltransferase) (rRNA (cytosine-N(4)-)-methyltransferase RsmH)

CENTER ID: BuceA.01545.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01545.a.B1.GE35019 full length 1 313
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01545.a.B1.PS01632 full length 1 313

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3604
RefSeq: YP_002232572.1
UniProt: B4E6K0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGNELRHRTV LLDEAVESLV TRPDGVYVDG TFGRGGHSRA VLARLAPAAR LIAFDKDPRA IETAQGIKDA RFSIVHDSFA SMRDALAARG VEKVSGVLLD LGVSSPQVDD PARGFSFRAD GPLDMRMDPT RGESAAEWLA RASVQELTEV IRDYGEERFA FQIAKALVAR RAESDRLGPL DTTGELAQIV GHVVKTREKG KDPATRTFQA IRIHVNQELA DLQVVLDAAL SLLEQGGRLV VISFHSLEDR IVKRFMQAHA SAPAVDRRLP IRAVDLPSPP LKIISRQFPS EAEVAANPRA RSAVMRIAER VTP
NT Sequence
atgggaaatg aattgcggca tcggacggtg ctgttggatg aggcggtcga gtcgctcgtg acgcgaccgg acggcgtgta cgtcgacggc acgttcgggc gcggcggcca tagccgcgcg gtgctcgcgc ggctggcgcc ggccgcgcgg ctgatcgcgt tcgacaagga tccgagggcg atcgagacgg cgcagggcat caaggatgcg cgcttctcga tcgtgcatga cagctttgct tcgatgcgcg acgcgcttgc ggcgcgcggc gtcgagaagg tgtcgggggt gttgctggac ctgggcgtgt cctcgccgca ggtggacgat ccggcgcgcg gcttcagttt ccgtgccgat ggtccgctgg acatgcgaat ggatccgacg cgcggcgagt cggcggccga atggctcgcg cgggcttcgg tgcaggaatt gacggaggtg atacgggatt atggggaaga acggtttgct tttcagattg caaaggcgct tgttgctcgc cgggcagagt ccgaccgtct tgggcctctc gacaccacgg gcgagcttgc ccaaatcgtg ggtcacgtcg tcaaaacccg tgagaagggc aaggatccgg caacccgcac ctttcaggct atacggattc acgtcaatca agagcttgcg gacctgcaag tcgtactaga cgcggcattg tcgttgctgg agcaaggggg gcggctggtg gtcatcagct ttcattcact cgaggaccgg atcgtcaaac gattcatgca ggcgcacgcg agtgcgcctg cggtcgatcg tcgcctgccg atccgcgccg tcgacctccc gagcccgccg ctcaagatca tcagccgcca gtttccgagc gaagcggaag tcgccgcgaa tccgcgcgcc cgctcggccg tgatgcgcat tgcggagcgc gtcacgccat ga
Details for BuceA.01545.a.B1.GE35019
HARVESTED ON: 3/19/2012
SEQUENCED ON: 3/22/2012
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG NELRHRTVLL DEAVESLVTR PDGVYVDGTF GRGGHSRAVL ARLAPAARLI AFDKDPRAIE TAQGIKDARF SIVHDSFASM RDALAARGVE KVSGVLLDLG VSSPQVDDPA RGFSFRADGP LDMRMDPTRG ESAAEWLARA SVQELTEVIR DYGEERFAFQ IAKALVARRA ESDRLGPLDT TGELAQIVGH VVKTREKGKD PATRTFQAIR IHVNQELADL QVVLDAALSL LEQGGRLVVI SFHSLEDRIV KRFMQAHASA PAVDRRLPIR AVDLPSPPLK IISRQFPSEA EVAANPRARS AVMRIAERVT P
Validated NT Sequence
tcttactcac ttatggcgtg acgcgctccg caatgcgcat cacggccgag cgggcgcgcg gattcgcggc gacttccgct tcgctcggaa actggcggct gatgatcttg agcggcgggc tcgggaggtc gacggcgcgg atcggcaggc gacgatcgac cgcaggcgca ctcgcgtgcg cctgcatgaa tcgtttgacg atccggtcct cgagtgaatg aaagctgatg accaccagcc gccccccttg ctccagcaac gacaatgccg cgtctagtac gacttgcagg tccgcaagct cttgattgac gtgaatccgt atagcctgaa aggtgcgggt tgccggatcc ttgcccttct cacgggtttt gacgacgtga cccacgattt gggcaagctc gcccgtggtg tcgagaggcc caagacggtc ggactctgcc cggcgagcaa caagcgcctt tgcaatctga aaagcaaacc gttcttcccc ataatcccgt atcacctccg tcaattcctg caccgaagcc cgcgcgagcc attcggccgc cgactcgccg cgcgtcggat ccattcgcat gtccagcgga ccatcggcac ggaaactgaa gccgcgcgcc ggatcgtcca cctgcggcga ggacacgccc aggtccagca acacccccga caccttctcg acgccgcgcg ccgcaagcgc gtcgcgcatc gaagcaaagc tgtcatgcac gatcgagaag cgcgcatcct tgatgccctg cgccgtctcg atcgccctcg gatccttgtc gaacgcgatc agccgcgcgg ccggcgccag ccgcgcgagc accgcgcggc tatggccgcc gcgcccgaac gtgccgtcga cgtacacgcc gtccggtcgc gtcacgagcg actcgaccgc ctcatccaac agcaccgtcc gatgccgcaa ttcatttccc atatggtggt ggtggtggtg agccatggta tatctccttc ttaa
Expected Protein Sequence
MAHHHHHHMG NELRHRTVLL DEAVESLVTR PDGVYVDGTF GRGGHSRAVL ARLAPAARLI AFDKDPRAIE TAQGIKDARF SIVHDSFASM RDALAARGVE KVSGVLLDLG VSSPQVDDPA RGFSFRADGP LDMRMDPTRG ESAAEWLARA SVQELTEVIR DYGEERFAFQ IAKALVARRA ESDRLGPLDT TGELAQIVGH VVKTREKGKD PATRTFQAIR IHVNQELADL QVVLDAALSL LEQGGRLVVI SFHSLEDRIV KRFMQAHASA PAVDRRLPIR AVDLPSPPLK IISRQFPSEA EVAANPRARS AVMRIAERVT P
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggaa atgaattgcg gcatcggacg gtgctgttgg atgaggcggt cgagtcgctc gtgacgcgac cggacggcgt gtacgtcgac ggcacgttcg ggcgcggcgg ccatagccgc gcggtgctcg cgcggctggc gccggccgcg cggctgatcg cgttcgacaa ggatccgagg gcgatcgaga cggcgcaggg catcaaggat gcgcgcttct cgatcgtgca tgacagcttt gcttcgatgc gcgacgcgct tgcggcgcgc ggcgtcgaga aggtgtcggg ggtgttgctg gacctgggcg tgtcctcgcc gcaggtggac gatccggcgc gcggcttcag tttccgtgcc gatggtccgc tggacatgcg aatggatccg acgcgcggcg agtcggcggc cgaatggctc gcgcgggctt cggtgcagga attgacggag gtgatacggg attatgggga agaacggttt gcttttcaga ttgcaaaggc gcttgttgct cgccgggcag agtccgaccg tcttgggcct ctcgacacca cgggcgagct tgcccaaatc gtgggtcacg tcgtcaaaac ccgtgagaag ggcaaggatc cggcaacccg cacctttcag gctatacgga ttcacgtcaa tcaagagctt gcggacctgc aagtcgtact agacgcggca ttgtcgttgc tggagcaagg ggggcggctg gtggtcatca gctttcattc actcgaggac cggatcgtca aacgattcat gcaggcgcac gcgagtgcgc ctgcggtcga tcgtcgcctg ccgatccgcg ccgtcgacct cccgagcccg ccgctcaaga tcatcagccg ccagtttccg agcgaagcgg aagtcgccgc gaatccgcgc gcccgctcgg ccgtgatgcg cattgcggag cgcgtcacgc catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for BuceA.01545.a.B1.PS01632
PURIFICATION DATe: 9/19/2012
CONCENTRATION: 38mg/ml
OBSERVED MW: 36kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 6
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG NELRHRTVLL DEAVESLVTR PDGVYVDGTF GRGGHSRAVL ARLAPAARLI AFDKDPRAIE TAQGIKDARF SIVHDSFASM RDALAARGVE KVSGVLLDLG VSSPQVDDPA RGFSFRADGP LDMRMDPTRG ESAAEWLARA SVQELTEVIR DYGEERFAFQ IAKALVARRA ESDRLGPLDT TGELAQIVGH VVKTREKGKD PATRTFQAIR IHVNQELADL QVVLDAALSL LEQGGRLVVI SFHSLEDRIV KRFMQAHASA PAVDRRLPIR AVDLPSPPLK IISRQFPSEA EVAANPRARS AVMRIAERVT P
Validated NT Sequence
tcttactcac ttatggcgtg acgcgctccg caatgcgcat cacggccgag cgggcgcgcg gattcgcggc gacttccgct tcgctcggaa actggcggct gatgatcttg agcggcgggc tcgggaggtc gacggcgcgg atcggcaggc gacgatcgac cgcaggcgca ctcgcgtgcg cctgcatgaa tcgtttgacg atccggtcct cgagtgaatg aaagctgatg accaccagcc gccccccttg ctccagcaac gacaatgccg cgtctagtac gacttgcagg tccgcaagct cttgattgac gtgaatccgt atagcctgaa aggtgcgggt tgccggatcc ttgcccttct cacgggtttt gacgacgtga cccacgattt gggcaagctc gcccgtggtg tcgagaggcc caagacggtc ggactctgcc cggcgagcaa caagcgcctt tgcaatctga aaagcaaacc gttcttcccc ataatcccgt atcacctccg tcaattcctg caccgaagcc cgcgcgagcc attcggccgc cgactcgccg cgcgtcggat ccattcgcat gtccagcgga ccatcggcac ggaaactgaa gccgcgcgcc ggatcgtcca cctgcggcga ggacacgccc aggtccagca acacccccga caccttctcg acgccgcgcg ccgcaagcgc gtcgcgcatc gaagcaaagc tgtcatgcac gatcgagaag cgcgcatcct tgatgccctg cgccgtctcg atcgccctcg gatccttgtc gaacgcgatc agccgcgcgg ccggcgccag ccgcgcgagc accgcgcggc tatggccgcc gcgcccgaac gtgccgtcga cgtacacgcc gtccggtcgc gtcacgagcg actcgaccgc ctcatccaac agcaccgtcc gatgccgcaa ttcatttccc atatggtggt ggtggtggtg agccatggta tatctccttc ttaa
Expressed Protein Sequence
MAHHHHHHMG NELRHRTVLL DEAVESLVTR PDGVYVDGTF GRGGHSRAVL ARLAPAARLI AFDKDPRAIE TAQGIKDARF SIVHDSFASM RDALAARGVE KVSGVLLDLG VSSPQVDDPA RGFSFRADGP LDMRMDPTRG ESAAEWLARA SVQELTEVIR DYGEERFAFQ IAKALVARRA ESDRLGPLDT TGELAQIVGH VVKTREKGKD PATRTFQAIR IHVNQELADL QVVLDAALSL LEQGGRLVVI SFHSLEDRIV KRFMQAHASA PAVDRRLPIR AVDLPSPPLK IISRQFPSEA EVAANPRARS AVMRIAERVT P
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGAA ATGAATTGCG GCATCGGACG GTGCTGTTGG ATGAGGCGGT CGAGTCGCTC GTGACGCGAC CGGACGGCGT GTACGTCGAC GGCACGTTCG GGCGCGGCGG CCATAGCCGC GCGGTGCTCG CGCGGCTGGC GCCGGCCGCG CGGCTGATCG CGTTCGACAA GGATCCGAGG GCGATCGAGA CGGCGCAGGG CATCAAGGAT GCGCGCTTCT CGATCGTGCA TGACAGCTTT GCTTCGATGC GCGACGCGCT TGCGGCGCGC GGCGTCGAGA AGGTGTCGGG GGTGTTGCTG GACCTGGGCG TGTCCTCGCC GCAGGTGGAC GATCCGGCGC GCGGCTTCAG TTTCCGTGCC GATGGTCCGC TGGACATGCG AATGGATCCG ACGCGCGGCG AGTCGGCGGC CGAATGGCTC GCGCGGGCTT CGGTGCAGGA ATTGACGGAG GTGATACGGG ATTATGGGGA AGAACGGTTT GCTTTTCAGA TTGCAAAGGC GCTTGTTGCT CGCCGGGCAG AGTCCGACCG TCTTGGGCCT CTCGACACCA CGGGCGAGCT TGCCCAAATC GTGGGTCACG TCGTCAAAAC CCGTGAGAAG GGCAAGGATC CGGCAACCCG CACCTTTCAG GCTATACGGA TTCACGTCAA TCAAGAGCTT GCGGACCTGC AAGTCGTACT AGACGCGGCA TTGTCGTTGC TGGAGCAAGG GGGGCGGCTG GTGGTCATCA GCTTTCATTC ACTCGAGGAC CGGATCGTCA AACGATTCAT GCAGGCGCAC GCGAGTGCGC CTGCGGTCGA TCGTCGCCTG CCGATCCGCG CCGTCGACCT CCCGAGCCCG CCGCTCAAGA TCATCAGCCG CCAGTTTCCG AGCGAAGCGG AAGTCGCCGC GAATCCGCGC GCCCGCTCGG CCGTGATGCG CATTGCGGAG CGCGTCACGC CATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT