BuceA.01471.a
Aromatic amino acid aminotransferase (EC 2.6.1.57)
| CENTER ID: | BuceA.01471.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.01471.a.B1.GE36630 | full length | 1 | 400 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.01471.a.B1.PS01824 | full length | 1 | 400 | ||
| BuceA.01471.a.B1.PS01825 | full length | 1 | 400 |
Structures
4WD2
DEPOSITED: 9/6/2014
DETERMINATION: XRay
CLONE: BuceA.01471.a.B1.GE36630
PROTEIN: BuceA.01471.a.B1.PS01825
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|216591.5.peg.5227 |
| RefSeq: | YP_002234091.1 |
| UniProt: | B4EJ31 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MFEHIDAYPG DPILTLNENF QKDPRDQKVN LSIGIYFDAE GRIPVMGAVR EAETALQRDS GPKPYLPMVG LAAYRDAVQS LVFGADHPAR AAGRIATLQT LGGSGALKVG ADFLKRYFPD SQVWLSDPSW ENHRFIFERA GFTVNTYPYY DEATGGLKFD AMLAAIDALP ARSIVLLHAC CHNPTGVDLD EGQWEKLIDV IEARELLPFV DMAYQGFGAG LDADAFAVRE LARRGVPTLV ANSFSKNFSL YGERVGGLSV VCEDAAAAER VLGQLAGAVR SNYSNPQTYG AKVVAAVLGT PALRKQWEEE LSAMCRRIAR MRQSIHDGLR DHVSGEALTR YVKQRGMFTY TGLTESQVDA LREVHGVYIL RSGRMCVAGL NDSNVGIVAD AIGKVLKSGA
NT Sequence
atgttcgaac acatcgacgc gtacccgggc gacccgatcc tgacgctcaa cgagaacttc cagaaagatc cgcgcgacca gaaggtcaac ctgagcatcg ggatctattt cgacgctgaa ggccggattc cggtgatggg cgcggtacgc gaagccgaga ccgcgctgca gcgcgacagc ggcccgaagc cgtacctgcc gatggtcggc ctggccgcgt atcgcgacgc cgtgcagtcg ctggtgttcg gcgccgatca tccggcccgc gcggccggcc gcatcgcgac gctgcagacg ctcggtggct cgggcgcgct gaaggtcggc gccgatttcc tgaagcgcta ttttccggat tcgcaagtgt ggctgagcga cccgagctgg gaaaaccatc gcttcatctt cgagcgcgcc ggcttcacgg tgaacacgta tccgtactac gacgaggcga cgggcggcct gaagttcgac gcgatgctcg cggcgatcga cgcgctgccg gcgcgcagca tcgtgctgct gcacgcgtgc tgccacaacc cgaccggcgt cgacctcgac gaaggccagt gggagaagct gatcgacgtg atcgaggcgc gcgagctgct gccgttcgtc gacatggcgt accagggctt cggcgcgggc ctcgacgcgg atgcgttcgc ggtgcgcgag ctggcccgcc gcggcgtgcc gacgctcgtc gcgaactcgt tctcgaagaa cttctcgctg tatggcgagc gcgtgggcgg gttgagcgtc gtctgcgaag acgcagccgc ggccgagcgc gtgctcggcc agctggccgg cgccgtgcgc tcgaactaca gcaatccgca gacctacggc gcgaaggtcg tcgcggccgt gctcggcacg ccggcgctgc gcaagcagtg ggaagaggaa ctgtcggcga tgtgccgccg catcgcccgg atgcgccagt cgatccacga cggcctgcgc gaccacgtga gcggcgaagc gctcacgcgc tacgtgaagc agcgcgggat gttcacgtac acgggcctga ccgaatcgca ggtcgacgcg ctgcgcgagg tgcacggcgt gtacatcctg cgctcgggcc ggatgtgcgt ggcgggcctg aacgactcga acgtcggcat cgtcgcggac gcgatcggca aggtgctgaa gagcggcgcc tga
Details for BuceA.01471.a.B1.GE36630
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 2/14/2013 |
| EXPECTED MW: | 45kDa |
| OBSERVED MW: | 45kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 84 |
Validated AA Sequence
MGAVREAETA LQRDSGPKPY LPMVGLAAYR DAVQSLVFGA DHPARAAGRI ATLQTLGGSG ALKVGADFLK RYFPDSQVWL SDPSWENHRF IFERAGFTVN TYPYYDEATG GLKFDAMLAA IDALPARSIV LLHACCHNPT GVDLDEGQWE KLIDVIEARE LLPFVDMAYQ GFGAGLDADA FAVRELARRG VPTLVANSFS KNFSLYGERV GGLSVVCEDA AAAERVLGQL AGAVRSNYSN PQTYGAKVVA AVLGTPALRK QWEEELSAMC RRIARMRQSI HDGLRDHVSG EALTRYVKQR GMFTYTGLTE SQVDALREVH GVYILRSGRM CVAGLNDSNV GIVADA
Validated NT Sequence
gatcgcgtcc gcgacgatgc cgacgttcga gtcgttcagg cccgccacgc acatccggcc cgagcgcagg atgtacacgc cgtgcacctc gcgcagcgcg tcgacctgcg attcggtcag gcccgtgtac gtgaacatcc cgcgctgctt cacgtagcgc gtgagcgctt cgccgctcac gtggtcgcgc aggccgtcgt ggatcgactg gcgcatccgg gcgatgcggc ggcacatcgc cgacagttcc tcttcccact gcttgcgcag cgccggcgtg ccgagcacgg ccgcgacgac cttcgcgccg taggtctgcg gattgctgta gttcgagcgc acggcgccgg ccagctggcc gagcacgcgc tcggccgcgg ctgcgtcttc gcagacgacg ctcaacccgc ccacgcgctc gccatacagc gagaagttct tcgagaacga gttcgcgacg agcgtcggca cgccgcggcg ggccagctcg cgcaccgcga acgcatccgc gtcgaggccc gcgccgaagc cctggtacgc catgtcgacg aacggcagca gctcgcgcgc ctcgatcacg tcgatcagct tctcccactg gccttcgtcg aggtcgacgc cggtcgggtt gtggcagcac gcgtgcagca gcacgatgct gcgcgccggc agcgcgtcga tcgccgcgag catcgcgtcg aacttcaggc cgcccgtcgc ctcgtcgtag tacggatacg tgttcaccgt gaagccggcg cgctcgaaga tgaagcgatg gttttcccag ctcgggtcgc tcagccacac ttgcgaatcc ggaaaatagc gcttcaggaa atcggcgccg accttcagcg cgcccgagcc accgagcgtc tgcagcgtcg cgatgcggcc ggccgcgcgg gccggatgat cggcgccgaa caccagcgac tgcacggcgt cgcgatacgc ggccaggccg accatcggca ggtacggctt cgggccgctg tcgcgctgca gcgcggtctc ggcttcgcgt accgcgccca tcaccggaat ccggccttca gcgtcgaaat agatcccgat gctcaggttg accttctggt cgcgcggatc tttctggaag ttctcgttga gcgtcaggat cgggtcgccc gggtacgcgt cgatgtg
Expected Protein Sequence
MAHHHHHHMF EHIDAYPGDP ILTLNENFQK DPRDQKVNLS IGIYFDAEGR IPVMGAVREA ETALQRDSGP KPYLPMVGLA AYRDAVQSLV FGADHPARAA GRIATLQTLG GSGALKVGAD FLKRYFPDSQ VWLSDPSWEN HRFIFERAGF TVNTYPYYDE ATGGLKFDAM LAAIDALPAR SIVLLHACCH NPTGVDLDEG QWEKLIDVIE ARELLPFVDM AYQGFGAGLD ADAFAVRELA RRGVPTLVAN SFSKNFSLYG ERVGGLSVVC EDAAAAERVL GQLAGAVRSN YSNPQTYGAK VVAAVLGTPA LRKQWEEELS AMCRRIARMR QSIHDGLRDH VSGEALTRYV KQRGMFTYTG LTESQVDALR EVHGVYILRS GRMCVAGLND SNVGIVADAI GKVLKSGA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttcg aacacatcga cgcgtacccg ggcgacccga tcctgacgct caacgagaac ttccagaaag atccgcgcga ccagaaggtc aacctgagca tcgggatcta tttcgacgct gaaggccgga ttccggtgat gggcgcggta cgcgaagccg agaccgcgct gcagcgcgac agcggcccga agccgtacct gccgatggtc ggcctggccg cgtatcgcga cgccgtgcag tcgctggtgt tcggcgccga tcatccggcc cgcgcggccg gccgcatcgc gacgctgcag acgctcggtg gctcgggcgc gctgaaggtc ggcgccgatt tcctgaagcg ctattttccg gattcgcaag tgtggctgag cgacccgagc tgggaaaacc atcgcttcat cttcgagcgc gccggcttca cggtgaacac gtatccgtac tacgacgagg cgacgggcgg cctgaagttc gacgcgatgc tcgcggcgat cgacgcgctg ccggcgcgca gcatcgtgct gctgcacgcg tgctgccaca acccgaccgg cgtcgacctc gacgaaggcc agtgggagaa gctgatcgac gtgatcgagg cgcgcgagct gctgccgttc gtcgacatgg cgtaccaggg cttcggcgcg ggcctcgacg cggatgcgtt cgcggtgcgc gagctggccc gccgcggcgt gccgacgctc gtcgcgaact cgttctcgaa gaacttctcg ctgtatggcg agcgcgtggg cgggttgagc gtcgtctgcg aagacgcagc cgcggccgag cgcgtgctcg gccagctggc cggcgccgtg cgctcgaact acagcaatcc gcagacctac ggcgcgaagg tcgtcgcggc cgtgctcggc acgccggcgc tgcgcaagca gtgggaagag gaactgtcgg cgatgtgccg ccgcatcgcc cggatgcgcc agtcgatcca cgacggcctg cgcgaccacg tgagcggcga agcgctcacg cgctacgtga agcagcgcgg gatgttcacg tacacgggcc tgaccgaatc gcaggtcgac gcgctgcgcg aggtgcacgg cgtgtacatc ctgcgctcgg gccggatgtg cgtggcgggc ctgaacgact cgaacgtcgg catcgtcgcg gacgcgatcg gcaaggtgct gaagagcggc gcctgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for BuceA.01471.a.B1.PS01824
| PURIFICATION DATe: | 5/16/2013 |
| CONCENTRATION: | 56.7mg/ml |
| OBSERVED MW: | 40kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 2 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 84 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGAVREAETA LQRDSGPKPY LPMVGLAAYR DAVQSLVFGA DHPARAAGRI ATLQTLGGSG ALKVGADFLK RYFPDSQVWL SDPSWENHRF IFERAGFTVN TYPYYDEATG GLKFDAMLAA IDALPARSIV LLHACCHNPT GVDLDEGQWE KLIDVIEARE LLPFVDMAYQ GFGAGLDADA FAVRELARRG VPTLVANSFS KNFSLYGERV GGLSVVCEDA AAAERVLGQL AGAVRSNYSN PQTYGAKVVA AVLGTPALRK QWEEELSAMC RRIARMRQSI HDGLRDHVSG EALTRYVKQR GMFTYTGLTE SQVDALREVH GVYILRSGRM CVAGLNDSNV GIVADA
Validated NT Sequence
gatcgcgtcc gcgacgatgc cgacgttcga gtcgttcagg cccgccacgc acatccggcc cgagcgcagg atgtacacgc cgtgcacctc gcgcagcgcg tcgacctgcg attcggtcag gcccgtgtac gtgaacatcc cgcgctgctt cacgtagcgc gtgagcgctt cgccgctcac gtggtcgcgc aggccgtcgt ggatcgactg gcgcatccgg gcgatgcggc ggcacatcgc cgacagttcc tcttcccact gcttgcgcag cgccggcgtg ccgagcacgg ccgcgacgac cttcgcgccg taggtctgcg gattgctgta gttcgagcgc acggcgccgg ccagctggcc gagcacgcgc tcggccgcgg ctgcgtcttc gcagacgacg ctcaacccgc ccacgcgctc gccatacagc gagaagttct tcgagaacga gttcgcgacg agcgtcggca cgccgcggcg ggccagctcg cgcaccgcga acgcatccgc gtcgaggccc gcgccgaagc cctggtacgc catgtcgacg aacggcagca gctcgcgcgc ctcgatcacg tcgatcagct tctcccactg gccttcgtcg aggtcgacgc cggtcgggtt gtggcagcac gcgtgcagca gcacgatgct gcgcgccggc agcgcgtcga tcgccgcgag catcgcgtcg aacttcaggc cgcccgtcgc ctcgtcgtag tacggatacg tgttcaccgt gaagccggcg cgctcgaaga tgaagcgatg gttttcccag ctcgggtcgc tcagccacac ttgcgaatcc ggaaaatagc gcttcaggaa atcggcgccg accttcagcg cgcccgagcc accgagcgtc tgcagcgtcg cgatgcggcc ggccgcgcgg gccggatgat cggcgccgaa caccagcgac tgcacggcgt cgcgatacgc ggccaggccg accatcggca ggtacggctt cgggccgctg tcgcgctgca gcgcggtctc ggcttcgcgt accgcgccca tcaccggaat ccggccttca gcgtcgaaat agatcccgat gctcaggttg accttctggt cgcgcggatc tttctggaag ttctcgttga gcgtcaggat cgggtcgccc gggtacgcgt cgatgtg
Expressed Protein Sequence
MAHHHHHHMF EHIDAYPGDP ILTLNENFQK DPRDQKVNLS IGIYFDAEGR IPVMGAVREA ETALQRDSGP KPYLPMVGLA AYRDAVQSLV FGADHPARAA GRIATLQTLG GSGALKVGAD FLKRYFPDSQ VWLSDPSWEN HRFIFERAGF TVNTYPYYDE ATGGLKFDAM LAAIDALPAR SIVLLHACCH NPTGVDLDEG QWEKLIDVIE ARELLPFVDM AYQGFGAGLD ADAFAVRELA RRGVPTLVAN SFSKNFSLYG ERVGGLSVVC EDAAAAERVL GQLAGAVRSN YSNPQTYGAK VVAAVLGTPA LRKQWEEELS AMCRRIARMR QSIHDGLRDH VSGEALTRYV KQRGMFTYTG LTESQVDALR EVHGVYILRS GRMCVAGLND SNVGIVADAI GKVLKSGA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTCG AACACATCGA CGCGTACCCG GGCGACCCGA TCCTGACGCT CAACGAGAAC TTCCAGAAAG ATCCGCGCGA CCAGAAGGTC AACCTGAGCA TCGGGATCTA TTTCGACGCT GAAGGCCGGA TTCCGGTGAT GGGCGCGGTA CGCGAAGCCG AGACCGCGCT GCAGCGCGAC AGCGGCCCGA AGCCGTACCT GCCGATGGTC GGCCTGGCCG CGTATCGCGA CGCCGTGCAG TCGCTGGTGT TCGGCGCCGA TCATCCGGCC CGCGCGGCCG GCCGCATCGC GACGCTGCAG ACGCTCGGTG GCTCGGGCGC GCTGAAGGTC GGCGCCGATT TCCTGAAGCG CTATTTTCCG GATTCGCAAG TGTGGCTGAG CGACCCGAGC TGGGAAAACC ATCGCTTCAT CTTCGAGCGC GCCGGCTTCA CGGTGAACAC GTATCCGTAC TACGACGAGG CGACGGGCGG CCTGAAGTTC GACGCGATGC TCGCGGCGAT CGACGCGCTG CCGGCGCGCA GCATCGTGCT GCTGCACGCG TGCTGCCACA ACCCGACCGG CGTCGACCTC GACGAAGGCC AGTGGGAGAA GCTGATCGAC GTGATCGAGG CGCGCGAGCT GCTGCCGTTC GTCGACATGG CGTACCAGGG CTTCGGCGCG GGCCTCGACG CGGATGCGTT CGCGGTGCGC GAGCTGGCCC GCCGCGGCGT GCCGACGCTC GTCGCGAACT CGTTCTCGAA GAACTTCTCG CTGTATGGCG AGCGCGTGGG CGGGTTGAGC GTCGTCTGCG AAGACGCAGC CGCGGCCGAG CGCGTGCTCG GCCAGCTGGC CGGCGCCGTG CGCTCGAACT ACAGCAATCC GCAGACCTAC GGCGCGAAGG TCGTCGCGGC CGTGCTCGGC ACGCCGGCGC TGCGCAAGCA GTGGGAAGAG GAACTGTCGG CGATGTGCCG CCGCATCGCC CGGATGCGCC AGTCGATCCA CGACGGCCTG CGCGACCACG TGAGCGGCGA AGCGCTCACG CGCTACGTGA AGCAGCGCGG GATGTTCACG TACACGGGCC TGACCGAATC GCAGGTCGAC GCGCTGCGCG AGGTGCACGG CGTGTACATC CTGCGCTCGG GCCGGATGTG CGTGGCGGGC CTGAACGACT CGAACGTCGG CATCGTCGCG GACGCGATCG GCAAGGTGCT GAAGAGCGGC GCCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT
Details for BuceA.01471.a.B1.PS01825
| PURIFICATION DATe: | 5/16/2013 |
| CONCENTRATION: | 37.2mg/ml |
| OBSERVED MW: | data unavailable |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 1 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 84 |
Protocol Notes
notes unavailable
Validated AA Sequence
MGAVREAETA LQRDSGPKPY LPMVGLAAYR DAVQSLVFGA DHPARAAGRI ATLQTLGGSG ALKVGADFLK RYFPDSQVWL SDPSWENHRF IFERAGFTVN TYPYYDEATG GLKFDAMLAA IDALPARSIV LLHACCHNPT GVDLDEGQWE KLIDVIEARE LLPFVDMAYQ GFGAGLDADA FAVRELARRG VPTLVANSFS KNFSLYGERV GGLSVVCEDA AAAERVLGQL AGAVRSNYSN PQTYGAKVVA AVLGTPALRK QWEEELSAMC RRIARMRQSI HDGLRDHVSG EALTRYVKQR GMFTYTGLTE SQVDALREVH GVYILRSGRM CVAGLNDSNV GIVADA
Validated NT Sequence
gatcgcgtcc gcgacgatgc cgacgttcga gtcgttcagg cccgccacgc acatccggcc cgagcgcagg atgtacacgc cgtgcacctc gcgcagcgcg tcgacctgcg attcggtcag gcccgtgtac gtgaacatcc cgcgctgctt cacgtagcgc gtgagcgctt cgccgctcac gtggtcgcgc aggccgtcgt ggatcgactg gcgcatccgg gcgatgcggc ggcacatcgc cgacagttcc tcttcccact gcttgcgcag cgccggcgtg ccgagcacgg ccgcgacgac cttcgcgccg taggtctgcg gattgctgta gttcgagcgc acggcgccgg ccagctggcc gagcacgcgc tcggccgcgg ctgcgtcttc gcagacgacg ctcaacccgc ccacgcgctc gccatacagc gagaagttct tcgagaacga gttcgcgacg agcgtcggca cgccgcggcg ggccagctcg cgcaccgcga acgcatccgc gtcgaggccc gcgccgaagc cctggtacgc catgtcgacg aacggcagca gctcgcgcgc ctcgatcacg tcgatcagct tctcccactg gccttcgtcg aggtcgacgc cggtcgggtt gtggcagcac gcgtgcagca gcacgatgct gcgcgccggc agcgcgtcga tcgccgcgag catcgcgtcg aacttcaggc cgcccgtcgc ctcgtcgtag tacggatacg tgttcaccgt gaagccggcg cgctcgaaga tgaagcgatg gttttcccag ctcgggtcgc tcagccacac ttgcgaatcc ggaaaatagc gcttcaggaa atcggcgccg accttcagcg cgcccgagcc accgagcgtc tgcagcgtcg cgatgcggcc ggccgcgcgg gccggatgat cggcgccgaa caccagcgac tgcacggcgt cgcgatacgc ggccaggccg accatcggca ggtacggctt cgggccgctg tcgcgctgca gcgcggtctc ggcttcgcgt accgcgccca tcaccggaat ccggccttca gcgtcgaaat agatcccgat gctcaggttg accttctggt cgcgcggatc tttctggaag ttctcgttga gcgtcaggat cgggtcgccc gggtacgcgt cgatgtg
Expressed Protein Sequence
MAHHHHHHMF EHIDAYPGDP ILTLNENFQK DPRDQKVNLS IGIYFDAEGR IPVMGAVREA ETALQRDSGP KPYLPMVGLA AYRDAVQSLV FGADHPARAA GRIATLQTLG GSGALKVGAD FLKRYFPDSQ VWLSDPSWEN HRFIFERAGF TVNTYPYYDE ATGGLKFDAM LAAIDALPAR SIVLLHACCH NPTGVDLDEG QWEKLIDVIE ARELLPFVDM AYQGFGAGLD ADAFAVRELA RRGVPTLVAN SFSKNFSLYG ERVGGLSVVC EDAAAAERVL GQLAGAVRSN YSNPQTYGAK VVAAVLGTPA LRKQWEEELS AMCRRIARMR QSIHDGLRDH VSGEALTRYV KQRGMFTYTG LTESQVDALR EVHGVYILRS GRMCVAGLND SNVGIVADAI GKVLKSGA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTCG AACACATCGA CGCGTACCCG GGCGACCCGA TCCTGACGCT CAACGAGAAC TTCCAGAAAG ATCCGCGCGA CCAGAAGGTC AACCTGAGCA TCGGGATCTA TTTCGACGCT GAAGGCCGGA TTCCGGTGAT GGGCGCGGTA CGCGAAGCCG AGACCGCGCT GCAGCGCGAC AGCGGCCCGA AGCCGTACCT GCCGATGGTC GGCCTGGCCG CGTATCGCGA CGCCGTGCAG TCGCTGGTGT TCGGCGCCGA TCATCCGGCC CGCGCGGCCG GCCGCATCGC GACGCTGCAG ACGCTCGGTG GCTCGGGCGC GCTGAAGGTC GGCGCCGATT TCCTGAAGCG CTATTTTCCG GATTCGCAAG TGTGGCTGAG CGACCCGAGC TGGGAAAACC ATCGCTTCAT CTTCGAGCGC GCCGGCTTCA CGGTGAACAC GTATCCGTAC TACGACGAGG CGACGGGCGG CCTGAAGTTC GACGCGATGC TCGCGGCGAT CGACGCGCTG CCGGCGCGCA GCATCGTGCT GCTGCACGCG TGCTGCCACA ACCCGACCGG CGTCGACCTC GACGAAGGCC AGTGGGAGAA GCTGATCGAC GTGATCGAGG CGCGCGAGCT GCTGCCGTTC GTCGACATGG CGTACCAGGG CTTCGGCGCG GGCCTCGACG CGGATGCGTT CGCGGTGCGC GAGCTGGCCC GCCGCGGCGT GCCGACGCTC GTCGCGAACT CGTTCTCGAA GAACTTCTCG CTGTATGGCG AGCGCGTGGG CGGGTTGAGC GTCGTCTGCG AAGACGCAGC CGCGGCCGAG CGCGTGCTCG GCCAGCTGGC CGGCGCCGTG CGCTCGAACT ACAGCAATCC GCAGACCTAC GGCGCGAAGG TCGTCGCGGC CGTGCTCGGC ACGCCGGCGC TGCGCAAGCA GTGGGAAGAG GAACTGTCGG CGATGTGCCG CCGCATCGCC CGGATGCGCC AGTCGATCCA CGACGGCCTG CGCGACCACG TGAGCGGCGA AGCGCTCACG CGCTACGTGA AGCAGCGCGG GATGTTCACG TACACGGGCC TGACCGAATC GCAGGTCGAC GCGCTGCGCG AGGTGCACGG CGTGTACATC CTGCGCTCGG GCCGGATGTG CGTGGCGGGC CTGAACGACT CGAACGTCGG CATCGTCGCG GACGCGATCG GCAAGGTGCT GAAGAGCGGC GCCTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT