SSGCID: Target Page

BuceA.01095.a

Putative geranyltranstransferase (EC 2.5.1.10)

CENTER ID:	BuceA.01095.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.01095.a.B1.GE35015	full length		1	294

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.4625
RefSeq:	YP_002233528.1
UniProt:	B4EN28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTFEQWMRSV LDRVEDALGH YLPAETAMPA KLHEAMRYAV LGGGKRVRPL LCHAAGELTG ATEAARNAAA AALEMIHVYS LVHDDMPCMD DDALRRGKPT VHVQYDEPTA LLVGDALQSQ AFVALTDADA LSPVQQAALV RELALASGSI GMAGGQAIDL ASVGLKLTRE QLETMHRMKT GALLRAAVRM GALAGETPST ETMAALDVYA GAVGLAFQVV DDILDVTTDS ATLGKTAGKD AANDKPTYVS ILGLEASREL AAQLRAEAHD ALKPFGARAQ RLAELADLVV NRVS

NT Sequence

atgacattcg aacaatggat gcggtccgtg cttgaccgtg tcgaggacgc actcggccac tatctgccgg ctgaaaccgc gatgcccgcg aaacttcacg aggcgatgcg ctacgcggtc ctcggtggcg gcaaacgcgt tcgcccgctg ctgtgccatg cagcaggcga gctgaccggt gcgacggagg cggcacgcaa cgcggcggcg gcggcgctgg agatgatcca cgtctactcg ctcgtgcacg acgacatgcc gtgcatggac gacgacgcgc tgcgtcgcgg caagccgacc gtgcacgtgc agtacgacga gccgacggcg ctgctggtcg gcgatgcgct gcaatcgcag gcgttcgtcg cgctgaccga cgccgacgcg ctgtcgccgg tgcagcaggc ggcgctcgtg cgcgagctgg cgctcgcgag cggctcgatc ggcatggccg gcgggcaggc gatcgacctg gcgagcgtcg gcctgaagct gacgcgcgaa cagctcgaga cgatgcaccg gatgaagacg ggcgccttgc tgcgcgcggc cgtgcggatg ggtgcgctgg ccggcgagac gccttcgacg gaaacgatgg ctgcgctgga cgtgtacgcg ggggccgtgg gtctggcctt ccaggtcgtc gacgacattc tcgacgtcac gaccgattcg gcgacgcttg gcaagacggc cggcaaggac gcggcgaacg acaagccgac ctatgtatcg atcctcggtc tcgaggcgtc gcgcgagctg gcagcgcagc tgcgcgccga agcgcacgac gcgctgaaac cgttcggcgc acgcgcacag cgtctggccg aacttgccga cctggtagtg aaccgggtca gctga

Details for BuceA.01095.a.B1.GE35015

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	32kDa
OBSERVED MW:	32kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT FEQWMRSVLD RVEDALGHYL PAETAMPAKL HEAMRYAVLG GGKRVRPLLC HAAGELTGAT EAARNAAAAA LEMIHVYSLV HDDMPCMDDD ALRRGKPTVH VQYDEPTALL VGDALQSQAF VALTDADALS PVQQAALVRE LALASGSIGM AGGQAIDLAS VGLKLTREQL ETMHRMKTGA LLRAAVRMGA LAGETPSTET MAALDVYAGA VGLAFQVVDD ILDVTTDSAT LGKTAGKDAA NDKPTYVSIL GLEASRELAA QLRAEAHDAL KPFGARAQRL AELADLVVNR VS

Validated NT Sequence

tactcactta gctgacccgg ttcactacca ggtcggcaag ttcggccaga cgctgtgcgc gtgcgccgaa cggtttcagc gcgtcgtgcg cttcggcgcg cagctgcgct gccagctcgc gcgacgcctc gagaccgagg atcgatacat aggtcggctt gtcgttcgcc gcgtccttgc cggccgtctt gccaagcgtc gccgaatcgg tcgtgacgtc gagaatgtcg tcgacgacct ggaaggccag acccacggcc cccgcgtaca cgtccagcgc agccatcgtt tccgtcgaag gcgtctcgcc ggccagcgca cccatccgca cggccgcgcg cagcaaggcg cccgtcttca tccggtgcat cgtctcgagc tgttcgcgcg tcagcttcag gccgacgctc gccaggtcga tcgcctgccc gccggccatg ccgatcgagc cgctcgcgag cgccagctcg cgcacgagcg ccgcctgctg caccggcgac agcgcgtcgg cgtcggtcag cgcgacgaac gcctgcgatt gcagcgcatc gccgaccagc agcgccgtcg gctcgtcgta ctgcacgtgc acggtcggct tgccgcgacg cagcgcgtcg tcgtccatgc acggcatgtc gtcgtgcacg agcgagtaga cgtggatcat ctccagcgcc gccgccgccg cgttgcgtgc cgcctccgtc gcaccggtca gctcgcctgc tgcatggcac agcagcgggc gaacgcgttt gccgccaccg aggaccgcgt agcgcatcgc ctcgtgaagt ttcgcgggca tcgcggtttc agccggcaga tagtggccga gtgcgtcctc gacacggtca agcacggacc gcatccattg ttcgaatgtc atatggtggt ggtggtggtg agccatggta tanctccttc ntaaa

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacat tcgaacaatg gatgcggtcc gtgcttgacc gtgtcgagga cgcactcggc cactatctgc cggctgaaac cgcgatgccc gcgaaacttc acgaggcgat gcgctacgcg gtcctcggtg gcggcaaacg cgttcgcccg ctgctgtgcc atgcagcagg cgagctgacc ggtgcgacgg aggcggcacg caacgcggcg gcggcggcgc tggagatgat ccacgtctac tcgctcgtgc acgacgacat gccgtgcatg gacgacgacg cgctgcgtcg cggcaagccg accgtgcacg tgcagtacga cgagccgacg gcgctgctgg tcggcgatgc gctgcaatcg caggcgttcg tcgcgctgac cgacgccgac gcgctgtcgc cggtgcagca ggcggcgctc gtgcgcgagc tggcgctcgc gagcggctcg atcggcatgg ccggcgggca ggcgatcgac ctggcgagcg tcggcctgaa gctgacgcgc gaacagctcg agacgatgca ccggatgaag acgggcgcct tgctgcgcgc ggccgtgcgg atgggtgcgc tggccggcga gacgccttcg acggaaacga tggctgcgct ggacgtgtac gcgggggccg tgggtctggc cttccaggtc gtcgacgaca ttctcgacgt cacgaccgat tcggcgacgc ttggcaagac ggccggcaag gacgcggcga acgacaagcc gacctatgta tcgatcctcg gtctcgaggc gtcgcgcgag ctggcagcgc agctgcgcgc cgaagcgcac gacgcgctga aaccgttcgg cgcacgcgca cagcgtctgg ccgaacttgc cgacctggta gtgaaccggg tcagctgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat