BuceA.01059.a

Methionyl-tRNA formyltransferase (EC 2.1.2.9)

CENTER ID: BuceA.01059.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.01059.a.B1.GE43981 full length 1 330
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.486
RefSeq: YP_002229628.1
UniProt: B4E7V8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTHTLRVIFA GTPEFAAAAL AAIHEAGFPV PLVLTQPDRP AGRGMKLQAS AVKRYAVEHG MTVAQPPSLR RAGKYPAEAA DAIELLRTTQ HDVMVVAAYG LLLPQEVLDI PRAGCINIHA SLLPRWRGAA PIHRAIEAGD AETGVTLMQM DVGLDTGAMI EEARIAIAPD DTTATLHDRL AADGARLIVD ALVRLERDGT LPATPQPADG VTYAEKIGKH EAALDWRKPA DVLARQVRAF DPFPGGVATL DGAAIKLWAA EPVAARGTMA NAAPGTIVEA APEGVVVACG SGALRVTQLQ KPGGKRLPAR EFLAGSPLAA GQRFALPDVD
NT Sequence
atgacccata cgttgcgcgt gatttttgcc ggcacgccgg aattcgccgc ggctgccctt gccgcgatcc acgaggccgg tttcccggtg ccgctcgtgc ttacccagcc cgatcgcccg gccggccgcg ggatgaaact ccaggccagt gccgtgaagc gctacgccgt cgagcacggg atgaccgtcg cgcaaccgcc gtcgctgcgt cgcgccggca agtacccggc ggaggcggcc gacgcgatcg aactgctgcg cacgacgcag cacgacgtga tggtggtggc cgcgtacggc ctgctgctgc cgcaggaagt gctcgacatc ccgcgcgcgg gctgcatcaa cattcacgcg tcgctgctgc cgcgctggcg cggcgccgcg ccgatccatc gggcgatcga ggccggcgac gccgagacgg gcgtcacgct gatgcagatg gacgtcggcc tcgacaccgg cgcgatgatc gaggaggcgc gcatcgcgat cgcgcccgac gatacgaccg cgacgctgca cgaccgcctc gccgccgacg gcgcgcggct gatcgtcgac gcgctcgtgc ggctcgagcg cgacggcacg ctgccggcga cgccgcagcc cgccgacggc gtgacctacg cggaaaagat cggcaagcac gaagcggcgc tcgactggcg caagccggcc gacgtactcg cgcgccaggt gcgtgcattc gatccgttcc cgggcggcgt cgcgacgctc gacggcgcgg ccatcaagct gtgggcggcc gagcccgtgg cggcgcgcgg cacgatggcc aacgcggcgc ccggcacgat cgtcgaagcc gcgccggaag gcgtggtggt cgcatgcggc agcggcgcgc tgcgcgtcac tcagctgcag aagccgggcg gcaagcggct gcccgcgcgc gaattcctcg ccggttcgcc gctcgccgcg ggccagcggt tcgcgctgcc cgacgtcgac tga
Details for BuceA.01059.a.B1.GE43981
HARVESTED ON: data unavailable
SEQUENCED ON: 12/16/2020
EXPECTED MW: 36kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 75
PERCENT COVERAGE: 5
Validated AA Sequence
MAHHHHHHMT HTLRCPTSTK
Validated NT Sequence
tttntnnnnt ttaagaagga gatataccat ggctcaccac caccaccacc atatgaccca tacgttgcgc tgcccgacgt cgactaagtg agtaagatag gatccggctg ctaacnaagc ccnnannaan nnnnaannnn nnnnngnnnn nnnnnnnnnn nnngnnggnn nnnnncganc anatnancnc gnnnannnnc nnnnnnannn ngagncnnnn nnngncnntc ntgnnngnng nnnnannnnc nncganngcg nngctcnngc nnatgnnngn gtgcnncncg ngnnnnngnn nnnnnngnnn nnnannnnnt ggannnnngn nnnnncannn cnnnnnncnn annnncngng nnanntanng cnnnnnnncc nnnngannnn nanccnnana cnaggcncnn tggnnnnncn nnnnnnntnn ntcggnccnn aangcannnn nnannnnann nnnnantnnn gncnnnnnnn ntnngnttgc gnnncnnnnn gngannnnnn annncagnnn ncnagnnncn tgnnnnnnnn nncncnanng anngcntgcn ggnnganctg nnnnngnntn nnaanngnnn nnnnangnnn nngnnncann nnnngnnnnn nnnnnnnnnn gnngncannn nnnntngggn cnagnnnnnn ngnannncnn ncnngnnnnn ngcgnnnnna ttnnngancn nggggngngg nntgcggntc nnanctattc nnncnngnng gngnnnannn nnnacnngnn nnnnnnnnnn natnnnncng nnantcnnnn nnnnctgccn nnnnnngctt nnnnctcnnn ngnccnccct canancnnan nngnnnnatt nncgannnaa ncnnantcnn ntcnancncn ncnccnnncn ncnnnnnnnn nntngcnann nnnnncnccn cnannnnnaa ntnnga
Expected Protein Sequence
MAHHHHHHMT HTLRVIFAGT PEFAAAALAA IHEAGFPVPL VLTQPDRPAG RGMKLQASAV KRYAVEHGMT VAQPPSLRRA GKYPAEAADA IELLRTTQHD VMVVAAYGLL LPQEVLDIPR AGCINIHASL LPRWRGAAPI HRAIEAGDAE TGVTLMQMDV GLDTGAMIEE ARIAIAPDDT TATLHDRLAA DGARLIVDAL VRLERDGTLP ATPQPADGVT YAEKIGKHEA ALDWRKPADV LARQVRAFDP FPGGVATLDG AAIKLWAAEP VAARGTMANA APGTIVEAAP EGVVVACGSG ALRVTQLQKP GGKRLPAREF LAGSPLAAGQ RFALPDVD
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc atacgttgcg cgtgattttt gccggcacgc cggaattcgc cgcggctgcc cttgccgcga tccacgaggc cggtttcccg gtgccgctcg tgcttaccca gcccgatcgc ccggccggcc gcgggatgaa actccaggcc agtgccgtga agcgctacgc cgtcgagcac gggatgaccg tcgcgcaacc gccgtcgctg cgtcgcgccg gcaagtaccc ggcggaggcg gccgacgcga tcgaactgct gcgcacgacg cagcacgacg tgatggtggt ggccgcgtac ggcctgctgc tgccgcagga agtgctcgac atcccgcgcg cgggctgcat caacattcac gcgtcgctgc tgccgcgctg gcgcggcgcc gcgccgatcc atcgggcgat cgaggccggc gacgccgaga cgggcgtcac gctgatgcag atggacgtcg gcctcgacac cggcgcgatg atcgaggagg cgcgcatcgc gatcgcgccc gacgatacga ccgcgacgct gcacgaccgc ctcgccgccg acggcgcgcg gctgatcgtc gacgcgctcg tgcggctcga gcgcgacggc acgctgccgg cgacgccgca gcccgccgac ggcgtgacct acgcggaaaa gatcggcaag cacgaagcgg cgctcgactg gcgcaagccg gccgacgtac tcgcgcgcca ggtgcgtgca ttcgatccgt tcccgggcgg cgtcgcgacg ctcgacggcg cggccatcaa gctgtgggcg gccgagcccg tggcggcgcg cggcacgatg gccaacgcgg cgcccggcac gatcgtcgaa gccgcgccgg aaggcgtggt ggtcgcatgc ggcagcggcg cgctgcgcgt cactcagctg cagaagccgg gcggcaagcg gctgcccgcg cgcgaattcc tcgccggttc gccgctcgcc gcgggccagc ggttcgcgct gcccgacgtc gactgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat