BuceA.00941.a

Chromosomal replication initiator protein DnaA

CENTER ID: BuceA.00941.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00941.a.B1.GE43979 full length 1 525
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.440
RefSeq: YP_002229585.1
UniProt: B4E7D1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNDFWQHCSA LLERELTPQQ YVTWIKPLAP VAFDASANTL SIAAPNRFKL DWVKSQFSGR IADLAREFWN TPIEVQFVLD PKAGMRSAPA GAAPAAPRAP LAPNGPAATV AAIAANLTAN ASAAPAAPAD VPMTPSAAAA RHLNADDADI DLPSLPAHEA AAGRRTWRPG PGAAPANGGE ADSMYERSKL NPVLTFDNFV TGKANQLARA AAIQVADNPG ISYNPLFLYG GVGLGKTHLI HAIGNQLLLD KAGARIRYIH AEQYVSDVVK AYQRKAFDDF KRYYHSLDLL LIDDIQFFSG KSRTQEEFFY AFEALVANKA QVIITSDTYP KEISGIDDRL ISRFDSGLTV AIEPPELEMR VAILMRKAQS EGVNLSEDVA FFVAKHLRSN VRELEGALRK ILAYSKFHGR EISIELTKEA LKDLLTVQNR QISVENIQKT VADFYNIKVA DMYSKKRPAN IARPRQIAMY LAKELTQKSL PEIGELFGGR DHTTVLHAVR KIADERSKDA QLNHELHVLE QTLKG
NT Sequence
atgaacgatt tctggcaaca ctgttccgca ctgctggagc gcgagctgac gccccagcag tacgtgacgt ggatcaaacc cttggccccg gtggccttcg atgcgtcggc gaacacgctg tccatcgccg cgccgaaccg cttcaagctg gactgggtca agagccagtt ttcgggccgg atcgccgatc tggcccgcga attctggaac acgccgatcg aagtccagtt cgtcctcgat ccgaaggccg gcatgcgcag cgcccccgca ggcgccgcac cggccgcccc gcgtgcaccg ctggccccga acggccccgc cgcgacggtc gccgcgatcg ccgccaacct gaccgcgaat gcttccgctg cgcccgccgc gccggccgac gtgccgatga cgccgtcggc ggccgccgcg cgccacctga acgccgacga cgccgacatc gacctgccga gcctgcccgc gcacgaagcg gcggccggcc gccgcacgtg gcgtccgggc ccgggcgccg cgcccgcgaa cggcggcgaa gccgattcga tgtacgaacg ctcgaagctg aacccggtgc tcacgttcga caacttcgtg accggtaaag ccaaccagct cgcgcgcgcg gccgcgatcc aggtcgcgga caatcccggc atctcgtaca acccgctgtt cctgtacggc ggcgtcggcc tcggcaagac ccacctgatc cacgcgatcg gcaaccagtt gctgctcgac aaggccggcg cgcggatccg ctacatccac gccgagcaat acgtgtcgga cgtggtgaag gcgtaccagc gcaaggcgtt cgacgacttc aagcgctact accactcgct cgacctgctg ctgatcgacg atatccagtt cttctccggc aagtcgcgca cgcaagagga attcttctac gcgttcgagg cgctcgtcgc gaacaaggcg caggtgatca tcaccagcga cacgtatccg aaggagattt cgggcatcga cgatcgcctg atctcgcgct tcgattcggg cctgaccgtc gcgatcgagc cgcccgagct ggaaatgcgc gtcgcgatcc tgatgcgcaa ggcgcagtcg gaaggcgtga acctgtcgga agacgtcgcg ttcttcgtcg cgaagcacct gcgctcgaac gtgcgcgagc tcgaaggcgc gctgcgcaag atcctcgcgt attcgaagtt ccacggccgc gagatctcga tcgagctgac caaggaagcg ctgaaggacc tgctgaccgt gcagaaccgg cagatttcgg tcgagaacat ccagaagacc gtcgccgact tctacaacat caaggtcgcg gacatgtact cgaagaagcg tcccgcgaac atcgcgcggc cgcggcagat tgcgatgtac ctggcgaagg aactcacgca gaagagcctg ccggaaatcg gcgagctgtt cggcgggcgc gatcacacca ccgtgctgca cgcggtgcgc aagatcgccg acgagcgcag caaggacgcg cagctcaacc acgagctgca cgtgctggaa cagacgctga agggctga
Details for BuceA.00941.a.B1.GE43979
HARVESTED ON: data unavailable
SEQUENCED ON: 12/16/2020
EXPECTED MW: 59kDa
OBSERVED MW: 30kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
XAXXHHXHMN DXWQHCSALL ERELTPQQYV TWIKPLAPVA FDASANTLSI AAPNRFKLDW VKSQFSGRIA DLAREFWNTP IEVQFVLDPK AGMRSAPAGA APAAPRAPLA PNGPAATVAA IAANLTANAS AAPAAPADVP MTPSAAAARH LNADDADIDL PSLPAHEAAA GRRTWRPGPG AAPANGGEAD SMYERSKLNP VLTFDNFVTG KANQLARAAA IQVADNPGIS YNPLFLYGGV GLGKTHLIHA IGNQLLLDKA GARIRYIHAE QYVSDVVKAY QRKAFDDFKR YYHSLDLLLI DDIQFFSGKS RTQEEFFYAF EALVANKAQV IITSDTYPKE ISGIDDRLIS RFDSGLTVAI EPPELEMRVA ILMRKAQSEG VNLSEDVAFF VAKHLRSNVR ELEGALRKIL AYSKFHGREI SIELTKEALK DLLTVQNRQI SVENIQKTVA DFYNIKVADM YSKKRPANIA RPRQIAMYLA KELTQKSLPE IGELFGGRDH TTVLHAVRKI ADERSKDAQL NHELHVLEQT LKG
Validated NT Sequence
atggctcacc accaccacca ccatatgaac gatttctggc aacactgttc cgcactgctg gagcgcgagc tgacgcccca gcagtacgtg acgtggatca aacccttggc cccggtggcc ttcgatgcgt cggcgaacac gctgtccatc gccgcgccga accgcttcaa gctggactgg gtcaagagcc agttttcggg ccggatcgcc gatctggccc gcgaattctg gaacacgccg atcgaagtcc agttcgtcct cgatccgaag gccggcatgc gcagcgcccc cgcaggcgcc gcaccggccg ccccgcgtgc accgctggcc ccgaacggcc ccgccgcgac ggtcgccgcg atcgccgcca acctgaccgc gaatgcttcc gctgcgcccg ccgcgccggc cgacgtgccg atgacgccgt cggcggccgc cgcgcgccac ctgaacgccg acgacgccga catcgacctg ccgagcctgc ccgcgcacga agcggcggcc ggccgccgca cgtggcgtcc gggcccgggc gccgcgcccg cgaacggcgg cgaagccgat tcgatgtacg aacgctcgaa gctgaacccg gtgctcacgt tcgacaactt cgtgaccggt aaagccaacc agctcgcgcg cgcggccgcg atccaggtcg cggacaatcc cggcatctcg tacaacccgc tgttcctgta cggcggcgtc ggcctcggca agacccacct gatccacgcg atcggcaacc agttgctgct cgacaaggcc ggcgcgcgga tccgctacat ccacgccgag caatacgtgt cggacgtggt gaaggcgtac cagcgcaagg gngttcgacg acttcaagcg ctactaccac tcgctcgacc tgctgctgat cgacgatatc cagttcttct ccggcaagtc gcgcacgcaa gaggaattct tctacgcgtt cgaggcgctc gtcgcgaaca aggcgcaggt gatcatcacc agcgacacgt atccgaagga gatttcgggc atcgacgatc gcctgatctc gcgcttcgat tcgggcctga ccgtcgcgat cgagccgccc gagctggaaa tgcgcgtcgc gatcctgatg cgcaaggcgc agtcggaagg cgtgaacctg tcggaagacg tcgcgttctt cgtcgcgaag cacctgcgct cgaacgtgcg cgagctcgaa ggcgcgctgc gcaagatcct cgcgtattcg aagttccacg gccgcgagat ctcgatcgag ctgaccaagg aagcgctgaa ggacctgctg accgtgcaga accggcagat ttcggtcgag aacatccaga agaccgtcgc cgacttctac aacatcaagg tcgcggacat gtactcgaag aagcgtcccg cgaacatcgc gcggccgcgg cagattgcga tgtacctggc gaaggaactc acgcagaaga gcctgccgga aatcggcgag ctgttcggcg ggcgcgatca caccaccgtg ctgcacgcgg tgcgcaagat cgccgacgag cgcagcaagg acgcgcagct caaccacgag ctgcacgtgc tggaacagac gnctgaaggg ctaagtgagt aagat
Expected Protein Sequence
MAHHHHHHMN DFWQHCSALL ERELTPQQYV TWIKPLAPVA FDASANTLSI AAPNRFKLDW VKSQFSGRIA DLAREFWNTP IEVQFVLDPK AGMRSAPAGA APAAPRAPLA PNGPAATVAA IAANLTANAS AAPAAPADVP MTPSAAAARH LNADDADIDL PSLPAHEAAA GRRTWRPGPG AAPANGGEAD SMYERSKLNP VLTFDNFVTG KANQLARAAA IQVADNPGIS YNPLFLYGGV GLGKTHLIHA IGNQLLLDKA GARIRYIHAE QYVSDVVKAY QRKAFDDFKR YYHSLDLLLI DDIQFFSGKS RTQEEFFYAF EALVANKAQV IITSDTYPKE ISGIDDRLIS RFDSGLTVAI EPPELEMRVA ILMRKAQSEG VNLSEDVAFF VAKHLRSNVR ELEGALRKIL AYSKFHGREI SIELTKEALK DLLTVQNRQI SVENIQKTVA DFYNIKVADM YSKKRPANIA RPRQIAMYLA KELTQKSLPE IGELFGGRDH TTVLHAVRKI ADERSKDAQL NHELHVLEQT LKG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg atttctggca acactgttcc gcactgctgg agcgcgagct gacgccccag cagtacgtga cgtggatcaa acccttggcc ccggtggcct tcgatgcgtc ggcgaacacg ctgtccatcg ccgcgccgaa ccgcttcaag ctggactggg tcaagagcca gttttcgggc cggatcgccg atctggcccg cgaattctgg aacacgccga tcgaagtcca gttcgtcctc gatccgaagg ccggcatgcg cagcgccccc gcaggcgccg caccggccgc cccgcgtgca ccgctggccc cgaacggccc cgccgcgacg gtcgccgcga tcgccgccaa cctgaccgcg aatgcttccg ctgcgcccgc cgcgccggcc gacgtgccga tgacgccgtc ggcggccgcc gcgcgccacc tgaacgccga cgacgccgac atcgacctgc cgagcctgcc cgcgcacgaa gcggcggccg gccgccgcac gtggcgtccg ggcccgggcg ccgcgcccgc gaacggcggc gaagccgatt cgatgtacga acgctcgaag ctgaacccgg tgctcacgtt cgacaacttc gtgaccggta aagccaacca gctcgcgcgc gcggccgcga tccaggtcgc ggacaatccc ggcatctcgt acaacccgct gttcctgtac ggcggcgtcg gcctcggcaa gacccacctg atccacgcga tcggcaacca gttgctgctc gacaaggccg gcgcgcggat ccgctacatc cacgccgagc aatacgtgtc ggacgtggtg aaggcgtacc agcgcaaggc gttcgacgac ttcaagcgct actaccactc gctcgacctg ctgctgatcg acgatatcca gttcttctcc ggcaagtcgc gcacgcaaga ggaattcttc tacgcgttcg aggcgctcgt cgcgaacaag gcgcaggtga tcatcaccag cgacacgtat ccgaaggaga tttcgggcat cgacgatcgc ctgatctcgc gcttcgattc gggcctgacc gtcgcgatcg agccgcccga gctggaaatg cgcgtcgcga tcctgatgcg caaggcgcag tcggaaggcg tgaacctgtc ggaagacgtc gcgttcttcg tcgcgaagca cctgcgctcg aacgtgcgcg agctcgaagg cgcgctgcgc aagatcctcg cgtattcgaa gttccacggc cgcgagatct cgatcgagct gaccaaggaa gcgctgaagg acctgctgac cgtgcagaac cggcagattt cggtcgagaa catccagaag accgtcgccg acttctacaa catcaaggtc gcggacatgt actcgaagaa gcgtcccgcg aacatcgcgc ggccgcggca gattgcgatg tacctggcga aggaactcac gcagaagagc ctgccggaaa tcggcgagct gttcggcggg cgcgatcaca ccaccgtgct gcacgcggtg cgcaagatcg ccgacgagcg cagcaaggac gcgcagctca accacgagct gcacgtgctg gaacagacgc tgaagggctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat