BuceA.00830.a

Bifunctional enzyme CysN/CysC [includes: sulfate adenylyltransferas subunit 1; adenylyl-sulfate kinase] (EC 2.7.1.25)

CENTER ID: BuceA.00830.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00830.a.A1.GE33207 full length 1 632
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2358
RefSeq: YP_002231386.1
UniProt: B4EDY9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAHVLAAPEA PPIHADETRT KDLLRFITCG SVDDGKSTLI GRLLYESNML FDDQLTQLEA DSKKVGTQGG ELDFALLVDG LSAEREQGIT IDVAYRFFAT ARRKFIVADT PGHEQYTRNM ITGASTADLA VILIDARKGV LTQTRRHSHL VALIGIKRVV LAINKMDLVD YDRAVFERID ADYRAFAAEL GFAEIVSIPM SALRGDNVIA PSARMPWYAG PPLMQHLDRL PLAARVTHDE PFRLPVQWVN RPHLNFRGYA GSIASGEIRV GERVRVLPSG KESRVASVIT PAGEAGIARA GEAVTLTLAD EIDISRGDLI ARADAPPEVA DQFEATLVWM HDEPLLPGRP YLVKLGTQTV GATCATPKYK IDVNTREHLA ARTLALNEIG VCNLSFDRPV AFDPYDRNRH TGGFIVIDRF SNDTVGAGML HFALRRAHNV HWQAVDVDRD ARARQKAQTP RIVWLTGLSG AGKSTIANLV EQRLHALGKH TYLLDGDNVR HGLNRDLGFT EADRVENIRR VAEVARLMLD AGLITLVSFI SPFRAERDMA RALAGPDEFV EVFVDTPLAV AEERDPKGLY KKARRGELKH FTGIDSPYEP PVQPELRVDT VTESPEVAAD RIVTYLLRER AA
NT Sequence
atggcacacg tactcgccgc gcccgaggcg ccccctatcc acgccgacga gacgcgaacg aaggacctgc tgcgtttcat cacctgcggc agcgtcgacg acggcaagag cacgctgatc ggccgcctgc tctacgaatc gaacatgctg ttcgacgacc agctcacgca gctcgaagcc gattcgaaga aggtcggcac gcagggcggc gaactcgatt tcgcgctgct cgtcgacggc ctgtcggccg aacgcgagca aggcatcacg atcgacgtcg cgtaccgctt cttcgcgacc gcgcggcgca agttcatcgt cgccgatacg cccggccacg aacagtacac gcgcaacatg atcaccggcg cgtcgaccgc cgatctcgcg gtgatcctga tcgacgcgcg caagggcgtg ctcacgcaga cgcgccgtca cagccatctc gtcgcgctga tcggcatcaa gcgcgtcgtg ctcgcgatca acaagatgga cctcgtcgac tacgaccggg ccgtgttcga gcgtatcgat gcggactacc gcgcgttcgc ggccgagctc gggttcgccg agatcgtcag cattccgatg tccgcgctgc gcggcgacaa tgtgatcgcg ccgagcgcac ggatgccgtg gtacgcgggc ccgccgctga tgcagcatct cgaccgcctg ccgctcgccg cgcgcgtgac gcacgacgaa ccgttccggc tgccggtgca atgggtcaac cgtccgcacc tgaatttccg cggctacgcg ggcagcatcg cgtcgggcga gattcgcgtc ggcgaacgcg tgcgcgtgct gccgtccggc aaggaaagcc gcgtcgcgtc ggtgatcacg ccggccggcg aagccggcat cgcccgcgcc ggcgaagcgg tgacgctcac gctcgccgac gaaatcgaca tcagccgcgg cgacctgatc gcgcgtgcgg acgcgccgcc ggaagtcgcc gaccagttcg aggcgacgct cgtgtggatg cacgacgagc cgctgctgcc cggccgcccg tatctcgtga agctcggcac gcagacggtc ggcgcgacct gcgcgacgcc gaagtacaag atcgacgtga acacgcgcga gcatctcgcc gcgcgcacgc tcgcgctgaa cgagatcggc gtgtgcaacc tgagcttcga ccggcccgtc gcgttcgatc cgtacgaccg gaaccgtcat acgggcggct tcatcgtgat cgaccgcttc agcaacgaca cggtcggcgc cgggatgctg cacttcgcgc tgcgccgcgc gcacaacgtg cactggcagg ccgtcgacgt cgatcgcgac gcgcgcgcgc ggcagaaggc gcagacgccg cgcatcgtgt ggctgacggg gctgtcgggc gccggcaagt cgacgatcgc gaacctcgtc gagcaacggc tgcacgcgct cggcaagcat acgtacctgc tcgacggcga caacgtgcgg cacggcctca accgcgatct cggcttcacc gaggccgacc gcgtggagaa catccggcgc gtcgccgaag tcgcgcggct gatgctcgac gcggggctca tcacgctcgt gtcgttcatc tcgccgttcc gcgccgagcg cgacatggcg cgcgcgctgg cgggcccgga cgaattcgtc gaagtgttcg tcgacacgcc gctcgcggtc gcggaggagc gcgatccgaa gggcttgtac aagaaggcgc ggcgcggcga gctgaagcac ttcaccggca tcgattcgcc gtacgagccg cccgtgcagc cggagttgcg tgtcgacacg gtgaccgagt cgccggaggt cgcggcggat cgcatcgtca cgtacctgct gcgcgaacgc gcggcgtaa
Details for BuceA.00830.a.A1.GE33207
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 72kDa
OBSERVED MW: 72kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 98
PERCENT COVERAGE: 45
Validated AA Sequence
MGTLEAQTQG PGSMAHVLAA PEAPPIHADE TRTKDLLRFI TCGSVDDGKS TLIGRLLYES NMLFDDQLTQ LEADSKKVGT QGGELDFALL VDGLSAEREQ GITIDVAYRF FATARRKFIV ADTPGHEQYT RNMITGASTA DLAVILIDAR KGVLTQTRRH SHLVALIGIK RVVLAINKMD LVDYDRAVFE RIDADYRAFA AELGFAEIVS IPMSALRGDX VIAPSARMPW YAGPPLMQHL DRLPLAARVT HDEPFRLPVQ WVNRPHLNFR GYAGSIASGE XXVGXRVRVL PSGKESXVA
Validated NT Sequence
ggctcatcac catcaccatc atatgggtac cctggaagct cagacccagg gtcctggttc gatggcacac gtactcgccg cgcccgaggc gccccctatc cacgccgacg agacgcgaac gaaggacctg ctgcgtttca tcacctgcgg cagcgtcgac gacggcaaga gcacgctgat cggccgcctg ctctacgaat cgaacatgct gttcgacgac cagctcacgc agctcgaagc cgattcgaag aaggtcggca cgcagggcgg cgaactcgat ttcgcgctgc tcgtcgacgg cctgtcggcc gaacgcgagc aaggcatcac gatcgacgtc gcgtaccgct tcttcgcgac cgcgcggcgc aagttcatcg tcgccgatac gcccggccac gaacagtaca cgcgcaacat gatcaccggc gcgtcgaccg ccgatctcgc ggtgatcctg atcgacgcgc gcaagggcgt gctcacgcag acgcgccgtc acagccatct cgtcgcgctg atcggcatca agcgcgtcgt gctcgcgatc aacaagatgg acctcgtcga ctacgaccgg gccgtgttcg agcgtatcga tgcggactac cgcgcgttcg cggccgagct cgggttcgcc gagatcgtca gcattccgat gtccgcgctg cgcggcgacn atgtgatcgc gccgagcgca cggatgccgt ggtacgcggg cccgccgctg atgcagcatc tcgaccgnct gccgctcgcc gcgcgcgtga cgcacgacga accgttccgg ctgccggtgc aatgggtcaa ccgtccgcac ctgaatttcc gcggctacgc gggcagcatc gcgtcgggcg agantcncgt cggcnaacgc gtgcgcgtgc tgccgtccgg caaggaaagc cncgtcgcgt c
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAHVLAAPE APPIHADETR TKDLLRFITC GSVDDGKSTL IGRLLYESNM LFDDQLTQLE ADSKKVGTQG GELDFALLVD GLSAEREQGI TIDVAYRFFA TARRKFIVAD TPGHEQYTRN MITGASTADL AVILIDARKG VLTQTRRHSH LVALIGIKRV VLAINKMDLV DYDRAVFERI DADYRAFAAE LGFAEIVSIP MSALRGDNVI APSARMPWYA GPPLMQHLDR LPLAARVTHD EPFRLPVQWV NRPHLNFRGY AGSIASGEIR VGERVRVLPS GKESRVASVI TPAGEAGIAR AGEAVTLTLA DEIDISRGDL IARADAPPEV ADQFEATLVW MHDEPLLPGR PYLVKLGTQT VGATCATPKY KIDVNTREHL AARTLALNEI GVCNLSFDRP VAFDPYDRNR HTGGFIVIDR FSNDTVGAGM LHFALRRAHN VHWQAVDVDR DARARQKAQT PRIVWLTGLS GAGKSTIANL VEQRLHALGK HTYLLDGDNV RHGLNRDLGF TEADRVENIR RVAEVARLML DAGLITLVSF ISPFRAERDM ARALAGPDEF VEVFVDTPLA VAEERDPKGL YKKARRGELK HFTGIDSPYE PPVQPELRVD TVTESPEVAA DRIVTYLLRE RAA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacacgta ctcgccgcgc ccgaggcgcc ccctatccac gccgacgaga cgcgaacgaa ggacctgctg cgtttcatca cctgcggcag cgtcgacgac ggcaagagca cgctgatcgg ccgcctgctc tacgaatcga acatgctgtt cgacgaccag ctcacgcagc tcgaagccga ttcgaagaag gtcggcacgc agggcggcga actcgatttc gcgctgctcg tcgacggcct gtcggccgaa cgcgagcaag gcatcacgat cgacgtcgcg taccgcttct tcgcgaccgc gcggcgcaag ttcatcgtcg ccgatacgcc cggccacgaa cagtacacgc gcaacatgat caccggcgcg tcgaccgccg atctcgcggt gatcctgatc gacgcgcgca agggcgtgct cacgcagacg cgccgtcaca gccatctcgt cgcgctgatc ggcatcaagc gcgtcgtgct cgcgatcaac aagatggacc tcgtcgacta cgaccgggcc gtgttcgagc gtatcgatgc ggactaccgc gcgttcgcgg ccgagctcgg gttcgccgag atcgtcagca ttccgatgtc cgcgctgcgc ggcgacaatg tgatcgcgcc gagcgcacgg atgccgtggt acgcgggccc gccgctgatg cagcatctcg accgcctgcc gctcgccgcg cgcgtgacgc acgacgaacc gttccggctg ccggtgcaat gggtcaaccg tccgcacctg aatttccgcg gctacgcggg cagcatcgcg tcgggcgaga ttcgcgtcgg cgaacgcgtg cgcgtgctgc cgtccggcaa ggaaagccgc gtcgcgtcgg tgatcacgcc ggccggcgaa gccggcatcg cccgcgccgg cgaagcggtg acgctcacgc tcgccgacga aatcgacatc agccgcggcg acctgatcgc gcgtgcggac gcgccgccgg aagtcgccga ccagttcgag gcgacgctcg tgtggatgca cgacgagccg ctgctgcccg gccgcccgta tctcgtgaag ctcggcacgc agacggtcgg cgcgacctgc gcgacgccga agtacaagat cgacgtgaac acgcgcgagc atctcgccgc gcgcacgctc gcgctgaacg agatcggcgt gtgcaacctg agcttcgacc ggcccgtcgc gttcgatccg tacgaccgga accgtcatac gggcggcttc atcgtgatcg accgcttcag caacgacacg gtcggcgccg ggatgctgca cttcgcgctg cgccgcgcgc acaacgtgca ctggcaggcc gtcgacgtcg atcgcgacgc gcgcgcgcgg cagaaggcgc agacgccgcg catcgtgtgg ctgacggggc tgtcgggcgc cggcaagtcg acgatcgcga acctcgtcga gcaacggctg cacgcgctcg gcaagcatac gtacctgctc gacggcgaca acgtgcggca cggcctcaac cgcgatctcg gcttcaccga ggccgaccgc gtggagaaca tccggcgcgt cgccgaagtc gcgcggctga tgctcgacgc ggggctcatc acgctcgtgt cgttcatctc gccgttccgc gccgagcgcg acatggcgcg cgcgctggcg ggcccggacg aattcgtcga agtgttcgtc gacacgccgc tcgcggtcgc ggaggagcgc gatccgaagg gcttgtacaa gaaggcgcgg cgcggcgagc tgaagcactt caccggcatc gattcgccgt acgagccgcc cgtgcagccg gagttgcgtg tcgacacggt gaccgagtcg ccggaggtcg cggcggatcg catcgtcacg tacctgctgc gcgaacgcgc ggcgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa