SSGCID: Target Page

BuceA.00765.a

Flavohemoprotein

CENTER ID:	BuceA.00765.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00765.a.B1.GE40273	Full length(BuceA.00765.a)		1	402

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00765.a.B1.PS38238	Full length(BuceA.00765.a)		1	402

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.3408
UniProt:	B4EE07

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTHITADQMA RVKATAPVLA EHGATITKHF YQRMFARHPE LKNLFNQTHQ KTGSQPETLA KAVYAYAANI DNLGALGGAV SRISHKHASL NIRPEHYPIV GENLLASIVE VLGDAVDADT LEAWRVAYGQ LAQIMIGAEA DLYANAAWSG FRPFKVARKV RESDEITSFY LTPADGGEAP TFEPGQYVTV KRFVGELGVD QPRQYSLSDA PHGQWLRISV KREAGKPEAI PAGKVSMLMH DGVEEGAIVE VTAPMGDFSL KRDVDTPVVL ISGGVGITPM MSMASALIAA GSPRDVRFIH ACRSGAVHAF RDWLNDTVGE HANVKRTVLY ELVGPNDRVG IDHDLEGRLT PERVQQYALV ADADYYICGP VAFMKAQRDA LVALGVAPER VNTEIFGSGA LE

NT Sequence

ATGACCCACA TCACCGCTGA CCAGATGGCC CGTGTGAAGG CCACCGCCCC CGTCCTCGCC GAGCACGGCG CGACGATCAC GAAGCACTTC TACCAGCGCA TGTTCGCGCG TCATCCGGAA CTGAAGAACC TGTTCAACCA GACGCACCAG AAGACCGGCA GCCAGCCCGA GACGCTCGCG AAGGCCGTCT ACGCGTATGC GGCGAACATC GACAACCTCG GCGCGCTGGG CGGCGCGGTG TCGCGCATCT CGCACAAGCA CGCGAGCCTG AACATCCGCC CCGAGCATTA CCCGATCGTC GGCGAGAACC TGCTCGCATC GATCGTCGAG GTGCTCGGCG ACGCGGTCGA TGCCGACACG CTCGAAGCCT GGCGCGTCGC ATACGGCCAG CTCGCGCAGA TCATGATCGG CGCCGAGGCC GATCTGTACG CGAACGCCGC GTGGAGCGGC TTCCGTCCGT TCAAGGTCGC GCGCAAGGTG CGTGAAAGCG ACGAGATCAC GTCGTTCTAC CTGACGCCCG CCGACGGCGG CGAAGCACCG ACGTTCGAGC CGGGCCAGTA CGTCACGGTG AAGCGTTTCG TCGGCGAGCT CGGCGTCGAC CAGCCGCGCC AGTACAGCCT GTCCGATGCG CCGCACGGCC AGTGGCTGCG CATCTCGGTG AAGCGCGAGG CCGGCAAGCC GGAAGCGATT CCGGCCGGCA AGGTGTCGAT GCTGATGCAC GATGGCGTCG AAGAGGGCGC GATCGTCGAA GTCACCGCGC CGATGGGCGA CTTTTCGCTG AAGCGCGACG TGGATACGCC GGTCGTGCTG ATCTCGGGCG GCGTCGGCAT CACGCCGATG ATGTCGATGG CGTCGGCGCT GATCGCGGCG GGCAGCCCGC GCGACGTGCG GTTCATCCAC GCCTGCCGTT CGGGCGCGGT GCACGCGTTC CGCGACTGGC TGAACGACAC CGTCGGCGAG CACGCGAACG TGAAGCGCAC GGTGCTGTAC GAACTGGTCG GCCCGAACGA TCGCGTCGGC ATCGACCACG ACCTCGAAGG GCGCCTCACG CCGGAGCGCG TGCAGCAGTA CGCGCTGGTG GCGGACGCCG ATTACTACAT CTGCGGGCCG GTCGCGTTCA TGAAGGCCCA GCGCGACGCG CTCGTCGCGC TGGGCGTCGC GCCGGAGCGC GTGAATACCG AGATCTTCGG TTCGGGCGCG CTCGAG

Details for BuceA.00765.a.B1.GE40273

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/20/2016
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMT HITADQMARV KATAPVLAEH GATITKHFYQ RMFARHPELK NLFNQTHQKT GSQPETLAKA VYAYAANIDN LGALGGAVSR ISHKHASLNI RPEHYPIVGE NLLASIVEVL GDAVDADTLE AWRVAYGQLA QIMIGAEADL YANAAWSGFR PFKVARKVRE SDEITSFYLT PADGGEAPTF EPGQYVTVKR FVGELGVDQP RQYSLSDAPH GQWLRISVKR EAGKPEAIPA GKVSMLMHDG VEEGAIVEVT APMGDFSLKR DVDTPVVLIS GGVGITPMMS MASALIAAGS PRDVRFIHAC RSGAVHAFRD WLNDTVGEHA NVKRTVLYEL VGPNDRVGID HDLEGRLTPE RVQQYALVAD ADYYICGPVA FMKAQRDALV ALGVAPERVN TEIFGSGALE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccc acatcaccgc tgaccagatg gcccgtgtga aggccaccgc ccccgtcctc gccgagcacg gcgcgacgat cacgaagcac ttctaccagc gcatgttcgc gcgtcatccg gaactgaaga acctgttcaa ccagacgcac cagaagaccg gcagccagcc cgagacgctc gcgaaggccg tctacgcgta tgcggcgaac atcgacaacc tcggcgcgct gggcggcgcg gtgtcgcgca tctcgcacaa gcacgcgagc ctgaacatcc gccccgagca ttacccgatc gtcggcgaga acctgctcgc atcgatcgtc gaggtgctcg gcgacgcggt cgatgccgac acgctcgaag cctggcgcgt cgcatacggc cagctcgcgc agatcatgat cggcgccgag gccgatctgt acgcgaacgc cgcgtggagc ggcttccgtc cgttcaaggt cgcgcgcaag gtgcgtgaaa gcgacgagat cacgtcgttc tacctgacgc ccgccgacgg cggcgaagca ccgacgttcg agccgggcca gtacgtcacg gtgaagcgtt tcgtcggcga gctcggcgtc gaccagccgc gccagtacag cctgtccgat gcgccgcacg gccagtggct gcgcatctcg gtgaagcgcg aggccggcaa gccggaagcg attccggccg gcaaggtgtc gatgctgatg cacgatggcg tcgaagaggg cgcgatcgtc gaagtcaccg cgccgatggg cgacttttcg ctgaagcgcg acgtggatac gccggtcgtg ctgatctcgg gcggcgtcgg catcacgccg atgatgtcga tggcgtcggc gctgatcgcg gcgggcagcc cgcgcgacgt gcggttcatc cacgcctgcc gttcgggcgc ggtgcacgcg ttccgcgact ggctgaacga caccgtcggc gagcacgcga acgtgaagcg cacggtgctg tacgaactgg tcggcccgaa cgatcgcgtc ggcatcgacc acgacctcga agggcgcctc acgccggagc gcgtgcagca gtacgcgctg gtggcggacg ccgattacta catctgcggg ccggtcgcgt tcatgaaggc ccagcgcgac gcgctcgtcg cgctgggcgt cgcgccggag cgcgtgaata ccgagatctt cggttcgggc gcgctcgagt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat

Details for BuceA.00765.a.B1.PS38238

PURIFICATION DATe:	3/21/2017
CONCENTRATION:	12.84mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Low Expression
PROTEIN PURIFICATION BUFFER:	SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	25
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACCC ACATCACCGC TGACCAGATG GCCCGTGTGA AGGCCACCGC CCCCGTCCTC GCCGAGCACG GCGCGACGAT CACGAAGCAC TTCTACCAGC GCATGTTCGC GCGTCATCCG GAACTGAAGA ACCTGTTCAA CCAGACGCAC CAGAAGACCG GCAGCCAGCC CGAGACGCTC GCGAAGGCCG TCTACGCGTA TGCGGCGAAC ATCGACAACC TCGGCGCGCT GGGCGGCGCG GTGTCGCGCA TCTCGCACAA GCACGCGAGC CTGAACATCC GCCCCGAGCA TTACCCGATC GTCGGCGAGA ACCTGCTCGC ATCGATCGTC GAGGTGCTCG GCGACGCGGT CGATGCCGAC ACGCTCGAAG CCTGGCGCGT CGCATACGGC CAGCTCGCGC AGATCATGAT CGGCGCCGAG GCCGATCTGT ACGCGAACGC CGCGTGGAGC GGCTTCCGTC CGTTCAAGGT CGCGCGCAAG GTGCGTGAAA GCGACGAGAT CACGTCGTTC TACCTGACGC CCGCCGACGG CGGCGAAGCA CCGACGTTCG AGCCGGGCCA GTACGTCACG GTGAAGCGTT TCGTCGGCGA GCTCGGCGTC GACCAGCCGC GCCAGTACAG CCTGTCCGAT GCGCCGCACG GCCAGTGGCT GCGCATCTCG GTGAAGCGCG AGGCCGGCAA GCCGGAAGCG ATTCCGGCCG GCAAGGTGTC GATGCTGATG CACGATGGCG TCGAAGAGGG CGCGATCGTC GAAGTCACCG CGCCGATGGG CGACTTTTCG CTGAAGCGCG ACGTGGATAC GCCGGTCGTG CTGATCTCGG GCGGCGTCGG CATCACGCCG ATGATGTCGA TGGCGTCGGC GCTGATCGCG GCGGGCAGCC CGCGCGACGT GCGGTTCATC CACGCCTGCC GTTCGGGCGC GGTGCACGCG TTCCGCGACT GGCTGAACGA CACCGTCGGC GAGCACGCGA ACGTGAAGCG CACGGTGCTG TACGAACTGG TCGGCCCGAA CGATCGCGTC GGCATCGACC ACGACCTCGA AGGGCGCCTC ACGCCGGAGC GCGTGCAGCA GTACGCGCTG GTGGCGGACG CCGATTACTA CATCTGCGGG CCGGTCGCGT TCATGAAGGC CCAGCGCGAC GCGCTCGTCG CGCTGGGCGT CGCGCCGGAG CGCGTGAATA CCGAGATCTT CGGTTCGGGC GCGCTCGAGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT