BuceA.00715.a

tRNA-dihydrouridine synthase (EC 1.-.-.-)

CENTER ID: BuceA.00715.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00715.a.B1.GE35025 full length 1 355
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3457
RefSeq: YP_002232437.1
UniProt: B4EES2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPVIGPHVLR NNLFVAPMAG VTDRPFRQLC KRLGAGYAVS EMVASNAQLW KSAKTMRRAN HEGEVEPIAV QIAGADPEMM AEAARYNVDN GAQIIDINMG CPAKKVCNVA AGSALLQNEP LVQRIVEAVV AAVGTGPDAV PVTLKIRTGW DREHKNAITV ARLAEAAGIS MLTVHGRTRA DLYRGDAEYD TIAAVKAAVR IPVVANGDIT SPAKAKAVLD ATGADALMIG RAAQGRPWLF REIDHFLQTG ELLPPPRIDE IQHVMNEHLE DHYAFYGEFT GVRTARKHIG WYTRGLSGAN GFRHRMNTLD STREQLAAVN AFFEAQKALS DHLVYVDDEE ENGQGESDDH NQLAA
NT Sequence
atgcccgtta tcggccctca cgtattgcgt aacaacctgt tcgtcgcccc gatggccggg gtgacggatc gtccgttccg ccagctgtgc aaacggctgg gggccggtta cgccgtgtcc gagatggtcg cgtcgaacgc gcagctgtgg aaaagcgcga agacgatgcg gcgcgcgaac cacgaaggcg aagtcgagcc gatcgcggtg cagatcgccg gcgccgatcc ggagatgatg gccgaagcgg cccgctacaa cgtcgacaac ggcgcgcaga tcatcgacat caacatgggc tgcccggcga agaaggtctg caacgtcgcg gccggctccg cgctgctgca gaacgagccg ctcgtgcagc ggatcgtcga ggcggtcgtc gcggcggtcg gtaccgggcc cgatgcggtg cccgtcacgt tgaagatccg caccggctgg gaccgcgagc acaagaacgc gatcacggtc gcgcgcctgg ccgaggcggc cggcatctcg atgctgaccg tgcacggccg cacgcgcgcc gacctgtacc ggggcgacgc cgagtacgac acgatcgcgg ccgtgaaggc ggccgtgcgg attccggtcg tcgcgaacgg cgacatcacg tcgcccgcca aggcgaaggc cgtgctcgat gcaaccggcg ccgacgcgct gatgatcggt cgcgccgcgc aaggccggcc gtggctgttc cgcgaaatcg atcatttcct gcaaaccggc gagctgctgc ccccgccgcg gatcgacgag atccagcacg tgatgaacga acacctggaa gaccactacg ctttctatgg cgaattcacc ggcgtccgta ctgcgcgcaa gcacatcggc tggtacactc gcggcctttc cggtgccaac gggttccggc accgaatgaa cacgctcgat tccacccgcg agcaactcgc cgccgtcaat gcattcttcg aggcgcaaaa ggcgctgtcg gaccacctcg tctacgtcga cgacgaagag gaaaacggcc agggcgagtc ggacgaccat aaccagttag cagcatga
Details for BuceA.00715.a.B1.GE35025
HARVESTED ON: 3/19/2012
SEQUENCED ON: 3/22/2012
EXPECTED MW: 39kDa
OBSERVED MW: 39kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 90
Validated AA Sequence
MAGVTDRPFR QLCKRLGAGY AVSEMVASNA QLWKSAKTMR RANHEGEVEP IAVQIAGADP EMMAEAARYN VDNGAQIIDI NMGCPAKKVC NVAAGSALLQ NEPLVQRIVE AVVAAVGTGP DAVPVTLKIR TGWDREHKNA ITVARLAEAA GISMLTVHGR TRADLYRGDA EYDTIAAVKA AVRIPVVANG DITSPAKAKA VLDATGADAL MIGRAAQGRP WLFREIDHFL QTGELLPPPR IDEIQHVMNE HLEDHYAFYG EFTGVRTARK HIGWYTRGLS GANGFRHRMN TLDSTREQLA AVNAFFEAQK ALSDHLVYVD DEEENGQGE
Validated NT Sequence
actcgccctg gccgttttcc tcttcgtcgt cgacgtagac gaggtggtcc gacagcgcct tttgcgcctc gaagaatgca ttgacggcgg cgagttgctc gcgggtggaa tcgagcgtgt tcattcggtg ccggaacccg ttggcaccgg aaaggccgcg agtgtaccag ccgatgtgct tgcgcgcagt acggacgccg gtgaattcgc catagaaagc gtagtggtct tccaggtgtt cgttcatcac gtgctggatc tcgtcgatcc gcggcggggg cagcagctcg ccggtttgca ggaaatgatc gatttcgcgg aacagccacg gccggccttg cgcggcgcga ccgatcatca gcgcgtcggc gccggttgca tcgagcacgg ccttcgcctt ggcgggcgac gtgatgtcgc cgttcgcgac gaccggaatc cgcacggccg ccttcacggc cgcgatcgtg tcgtactcgg cgtcgccccg gtacaggtcg gcgcgcgtgc ggccgtgcac ggtcagcatc gagatgccgg ccgcctcggc caggcgcgcg accgtgatcg cgttcttgtg ctcgcggtcc cagccggtgc ggatcttcaa cgtgacgggc accgcatcgg gcccggtacc gaccgccgcg acgaccgcct cgacgatccg ctgcacgagc ggctcgttct gcagcagcgc ggagccggcc gcgacgttgc agaccttctt cgccgggcag cccatgttga tgtcgatgat ctgcgcgccg ttgtcgacgt tgtagcgggc cgcttcggcc atcatctccg gatcggcgcc ggcgatctgc accgcgatcg gctcgacttc gccttcgtgg ttcgcgcgcc gcatcgtctt cgcgcttttc cacagctgcg cgttcgacgc gaccatctcg gacacggcgt aaccggcccc cagccgtttg cacagctggc ggaacggacg atccgtcacc ccggccatcg gggcgacgaa caggttgtta cgcaatacgt gagggccgat
Expected Protein Sequence
MAHHHHHHMP VIGPHVLRNN LFVAPMAGVT DRPFRQLCKR LGAGYAVSEM VASNAQLWKS AKTMRRANHE GEVEPIAVQI AGADPEMMAE AARYNVDNGA QIIDINMGCP AKKVCNVAAG SALLQNEPLV QRIVEAVVAA VGTGPDAVPV TLKIRTGWDR EHKNAITVAR LAEAAGISML TVHGRTRADL YRGDAEYDTI AAVKAAVRIP VVANGDITSP AKAKAVLDAT GADALMIGRA AQGRPWLFRE IDHFLQTGEL LPPPRIDEIQ HVMNEHLEDH YAFYGEFTGV RTARKHIGWY TRGLSGANGF RHRMNTLDST REQLAAVNAF FEAQKALSDH LVYVDDEEEN GQGESDDHNQ LAA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgcccg ttatcggccc tcacgtattg cgtaacaacc tgttcgtcgc cccgatggcc ggggtgacgg atcgtccgtt ccgccagctg tgcaaacggc tgggggccgg ttacgccgtg tccgagatgg tcgcgtcgaa cgcgcagctg tggaaaagcg cgaagacgat gcggcgcgcg aaccacgaag gcgaagtcga gccgatcgcg gtgcagatcg ccggcgccga tccggagatg atggccgaag cggcccgcta caacgtcgac aacggcgcgc agatcatcga catcaacatg ggctgcccgg cgaagaaggt ctgcaacgtc gcggccggct ccgcgctgct gcagaacgag ccgctcgtgc agcggatcgt cgaggcggtc gtcgcggcgg tcggtaccgg gcccgatgcg gtgcccgtca cgttgaagat ccgcaccggc tgggaccgcg agcacaagaa cgcgatcacg gtcgcgcgcc tggccgaggc ggccggcatc tcgatgctga ccgtgcacgg ccgcacgcgc gccgacctgt accggggcga cgccgagtac gacacgatcg cggccgtgaa ggcggccgtg cggattccgg tcgtcgcgaa cggcgacatc acgtcgcccg ccaaggcgaa ggccgtgctc gatgcaaccg gcgccgacgc gctgatgatc ggtcgcgccg cgcaaggccg gccgtggctg ttccgcgaaa tcgatcattt cctgcaaacc ggcgagctgc tgcccccgcc gcggatcgac gagatccagc acgtgatgaa cgaacacctg gaagaccact acgctttcta tggcgaattc accggcgtcc gtactgcgcg caagcacatc ggctggtaca ctcgcggcct ttccggtgcc aacgggttcc ggcaccgaat gaacacgctc gattccaccc gcgagcaact cgccgccgtc aatgcattct tcgaggcgca aaaggcgctg tcggaccacc tcgtctacgt cgacgacgaa gaggaaaacg gccagggcga gtcggacgac cataaccagt tagcagcatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat