BuceA.00624.a

ABC transporter extracellular solute-binding protein

CENTER ID: BuceA.00624.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00624.a.B1.GE36626 full length 1 347
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00624.a.B1.PW37294 full length 1 347

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1146
RefSeq: YP_002230239.1
UniProt: B4ED28

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNPRTRLLP LAGALALAAA AFAPLAQAAE EVSLYTTREP KLIQPLIDAF TKQSGVKVNT VFVKDGLLER VKAEGAQSPA DVLMTVDVGN LLDLVDGGVT QPVRSKALDD AIPANLRATN GDWYALSLRD RVLYVEKDLK VDAFRYEDLA DPKWKGKVCI RSGQHPYNTA LVAAMIAHDG EAATETWLRG VKANLARKAT GGDRDVARDI LGGICDVGLA NAYYVGHMKH AEPGSDARKW GDAIKVVRPT FANAKSGGTH VNISGATVAK HAPHKANAVK LLEYLVSPEA QALYAQANYE YPVRANVKLD PVIASFGTLK VDPLPLADIA KHRKQASQLV DKVGFDN
NT Sequence
atgtcgaatc cgcgcacccg cctgctgccg ctcgccggcg ccctcgccct ggctgcagcc gccttcgcac cgcttgccca ggcggccgag gaagtgagcc tgtacaccac ccgcgagccg aagctgatcc agccgctcat cgacgcgttc acgaagcaga gcggcgtcaa ggtcaacacg gtgttcgtga aggacggcct gctcgaacgc gtgaaggcgg aaggcgcgca gtcgccggcc gacgtgctga tgacggtcga cgtcggcaac ctgctcgacc tcgtcgacgg cggcgtcacg cagccggtgc gctcgaaggc actcgacgac gcgattcccg cgaacctgcg cgccacgaac ggcgactggt acgcgctgtc gctgcgcgac cgcgtgctgt acgtcgaaaa agacctgaag gtcgatgcgt tccgttacga ggatctcgcc gatccgaaat ggaagggcaa ggtctgcatc cgctcgggcc agcatccgta caacaccgcg ctcgtcgcgg cgatgatcgc gcacgacggc gaggccgcaa ccgagacatg gctgcgcggc gtgaaggcga acctggcgcg caaggcgacc ggcggcgacc gcgacgtcgc gcgcgacatc ctcggcggaa tctgcgacgt cggcctcgcg aacgcttact acgtcggcca catgaagcac gcggaaccgg gcagcgatgc gcgcaaatgg ggcgacgcga tcaaggtcgt gcgcccgacc ttcgcgaacg cgaagagcgg cggcacgcac gtgaacatca gcggcgcgac ggtggcgaag catgcgccgc acaaggccaa cgcggtgaaa ctgctcgaat acctggtgtc gccggaagcg caggcgctgt atgcgcaggc gaactacgaa tacccggtgc gcgcgaacgt gaagctcgac ccggtgatcg cgagcttcgg cacgctgaag gtcgatccgc tgccgctggc ggatatcgcg aagcatcgca agcaggcaag ccagctcgtc gacaaggtcg gctttgacaa ctga
Details for BuceA.00624.a.B1.GE36626
HARVESTED ON: 2/8/2013
SEQUENCED ON: 2/14/2013
EXPECTED MW: 38kDa
OBSERVED MW: 38kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 19
PERCENT COVERAGE: 92
Validated AA Sequence
MLRDIRQRQR IDLQRAEARD HRVELHVRAH RVFVVRLRIQ RLRFRRHQVF EQFHRVGLVR RMLRHRRAAD VHVRAAALRV REGRAHDLDR VAPFARIAAR FRVLHVADVV SVREADVADS AEDVARDVAV AAGRLARQVR LHAAQPCLGC GLAVVRDHRR DERGVVRMLA RADADLALPF RIGEILVTER IDLQVFFDVQ HAVAQRQRVP VAVRGAQVRG NRVVECLRAH RLRDAAVDEV EQVADVDRHQ HVGRRLRAFR LHAFEQAVLH EHRVDLDAAL LRERVDERLD QLRLAGGVQA HFLGRLGKRC EGGCSQGEGA GERQQAGA
Validated NT Sequence
gagctggctt gcctgcttgc gatgcttcgc gatatccgcc agcggcagcg gatcgacctt cagcgtgccg aagctcgcga tcaccgggtc gagcttcacg ttcgcgcgca ccgggtattc gtagttcgcc tgcgcataca gcgcctgcgc ttccggcgac accaggtatt cgagcagttt caccgcgttg gccttgtgcg gcgcatgctt cgccaccgtc gcgccgctga tgttcacgtg cgtgccgccg ctcttcgcgt tcgcgaaggt cgggcgcacg accttgatcg cgtcgcccca tttgcgcgca tcgctgcccg gttccgcgtg cttcatgtgg ccgacgtagt aagcgttcgc gaggccgacg tcgcagattc cgccgaggat gtcgcgcgcg acgtcgcggt cgccgccggt cgccttgcgc gccaggttcg ccttcacgcc gcgcagccat gtctcggttg cggcctcgcc gtcgtgcgcg atcatcgccg cgacgagcgc ggtgttgtac ggatgctggc ccgagcggat gcagaccttg cccttccatt tcggatcggc gagatcctcg taacggaacg catcgacctt caggtctttt tcgacgtaca gcacgcggtc gcgcagcgac agcgcgtacc agtcgccgtt cgtggcgcgc aggttcgcgg gaatcgcgtc gtcgagtgcc ttcgagcgca ccggctgcgt gacgccgccg tcgacgaggt cgagcaggtt gccgacgtcg accgtcatca gcacgtcggc cggcgactgc gcgccttccg ccttcacgcg ttcgagcagg ccgtccttca cgaacaccgt gttgaccttg acgccgctct gcttcgtgaa cgcgtcgatg agcggctgga tcagcttcgg ctcgcgggtg gtgtacaggc tcacttcctc ggccgcctgg gcaagcggtg cgaaggcggc tgcagccagg gcgagggcgc cggcgagcgg cagcaggcgg gtgcgcgg
Expected Protein Sequence
MAHHHHHHMS NPRTRLLPLA GALALAAAAF APLAQAAEEV SLYTTREPKL IQPLIDAFTK QSGVKVNTVF VKDGLLERVK AEGAQSPADV LMTVDVGNLL DLVDGGVTQP VRSKALDDAI PANLRATNGD WYALSLRDRV LYVEKDLKVD AFRYEDLADP KWKGKVCIRS GQHPYNTALV AAMIAHDGEA ATETWLRGVK ANLARKATGG DRDVARDILG GICDVGLANA YYVGHMKHAE PGSDARKWGD AIKVVRPTFA NAKSGGTHVN ISGATVAKHA PHKANAVKLL EYLVSPEAQA LYAQANYEYP VRANVKLDPV IASFGTLKVD PLPLADIAKH RKQASQLVDK VGFDN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcga atccgcgcac ccgcctgctg ccgctcgccg gcgccctcgc cctggctgca gccgccttcg caccgcttgc ccaggcggcc gaggaagtga gcctgtacac cacccgcgag ccgaagctga tccagccgct catcgacgcg ttcacgaagc agagcggcgt caaggtcaac acggtgttcg tgaaggacgg cctgctcgaa cgcgtgaagg cggaaggcgc gcagtcgccg gccgacgtgc tgatgacggt cgacgtcggc aacctgctcg acctcgtcga cggcggcgtc acgcagccgg tgcgctcgaa ggcactcgac gacgcgattc ccgcgaacct gcgcgccacg aacggcgact ggtacgcgct gtcgctgcgc gaccgcgtgc tgtacgtcga aaaagacctg aaggtcgatg cgttccgtta cgaggatctc gccgatccga aatggaaggg caaggtctgc atccgctcgg gccagcatcc gtacaacacc gcgctcgtcg cggcgatgat cgcgcacgac ggcgaggccg caaccgagac atggctgcgc ggcgtgaagg cgaacctggc gcgcaaggcg accggcggcg accgcgacgt cgcgcgcgac atcctcggcg gaatctgcga cgtcggcctc gcgaacgctt actacgtcgg ccacatgaag cacgcggaac cgggcagcga tgcgcgcaaa tggggcgacg cgatcaaggt cgtgcgcccg accttcgcga acgcgaagag cggcggcacg cacgtgaaca tcagcggcgc gacggtggcg aagcatgcgc cgcacaaggc caacgcggtg aaactgctcg aatacctggt gtcgccggaa gcgcaggcgc tgtatgcgca ggcgaactac gaatacccgg tgcgcgcgaa cgtgaagctc gacccggtga tcgcgagctt cggcacgctg aaggtcgatc cgctgccgct ggcggatatc gcgaagcatc gcaagcaggc aagccagctc gtcgacaagg tcggctttga caactgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for BuceA.00624.a.B1.PW37294
PURIFICATION DATe: 5/22/2013
CONCENTRATION: 17.4mg/ml
OBSERVED MW: 38kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 110µl
PERCENT IDENTITY: 19
PERCENT COVERAGE: 92
Protocol Notes
notes unavailable
Validated AA Sequence
MLRDIRQRQR IDLQRAEARD HRVELHVRAH RVFVVRLRIQ RLRFRRHQVF EQFHRVGLVR RMLRHRRAAD VHVRAAALRV REGRAHDLDR VAPFARIAAR FRVLHVADVV SVREADVADS AEDVARDVAV AAGRLARQVR LHAAQPCLGC GLAVVRDHRR DERGVVRMLA RADADLALPF RIGEILVTER IDLQVFFDVQ HAVAQRQRVP VAVRGAQVRG NRVVECLRAH RLRDAAVDEV EQVADVDRHQ HVGRRLRAFR LHAFEQAVLH EHRVDLDAAL LRERVDERLD QLRLAGGVQA HFLGRLGKRC EGGCSQGEGA GERQQAGA
Validated NT Sequence
gagctggctt gcctgcttgc gatgcttcgc gatatccgcc agcggcagcg gatcgacctt cagcgtgccg aagctcgcga tcaccgggtc gagcttcacg ttcgcgcgca ccgggtattc gtagttcgcc tgcgcataca gcgcctgcgc ttccggcgac accaggtatt cgagcagttt caccgcgttg gccttgtgcg gcgcatgctt cgccaccgtc gcgccgctga tgttcacgtg cgtgccgccg ctcttcgcgt tcgcgaaggt cgggcgcacg accttgatcg cgtcgcccca tttgcgcgca tcgctgcccg gttccgcgtg cttcatgtgg ccgacgtagt aagcgttcgc gaggccgacg tcgcagattc cgccgaggat gtcgcgcgcg acgtcgcggt cgccgccggt cgccttgcgc gccaggttcg ccttcacgcc gcgcagccat gtctcggttg cggcctcgcc gtcgtgcgcg atcatcgccg cgacgagcgc ggtgttgtac ggatgctggc ccgagcggat gcagaccttg cccttccatt tcggatcggc gagatcctcg taacggaacg catcgacctt caggtctttt tcgacgtaca gcacgcggtc gcgcagcgac agcgcgtacc agtcgccgtt cgtggcgcgc aggttcgcgg gaatcgcgtc gtcgagtgcc ttcgagcgca ccggctgcgt gacgccgccg tcgacgaggt cgagcaggtt gccgacgtcg accgtcatca gcacgtcggc cggcgactgc gcgccttccg ccttcacgcg ttcgagcagg ccgtccttca cgaacaccgt gttgaccttg acgccgctct gcttcgtgaa cgcgtcgatg agcggctgga tcagcttcgg ctcgcgggtg gtgtacaggc tcacttcctc ggccgcctgg gcaagcggtg cgaaggcggc tgcagccagg gcgagggcgc cggcgagcgg cagcaggcgg gtgcgcgg
Expressed Protein Sequence
MAHHHHHHMS NPRTRLLPLA GALALAAAAF APLAQAAEEV SLYTTREPKL IQPLIDAFTK QSGVKVNTVF VKDGLLERVK AEGAQSPADV LMTVDVGNLL DLVDGGVTQP VRSKALDDAI PANLRATNGD WYALSLRDRV LYVEKDLKVD AFRYEDLADP KWKGKVCIRS GQHPYNTALV AAMIAHDGEA ATETWLRGVK ANLARKATGG DRDVARDILG GICDVGLANA YYVGHMKHAE PGSDARKWGD AIKVVRPTFA NAKSGGTHVN ISGATVAKHA PHKANAVKLL EYLVSPEAQA LYAQANYEYP VRANVKLDPV IASFGTLKVD PLPLADIAKH RKQASQLVDK VGFDN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCGA ATCCGCGCAC CCGCCTGCTG CCGCTCGCCG GCGCCCTCGC CCTGGCTGCA GCCGCCTTCG CACCGCTTGC CCAGGCGGCC GAGGAAGTGA GCCTGTACAC CACCCGCGAG CCGAAGCTGA TCCAGCCGCT CATCGACGCG TTCACGAAGC AGAGCGGCGT CAAGGTCAAC ACGGTGTTCG TGAAGGACGG CCTGCTCGAA CGCGTGAAGG CGGAAGGCGC GCAGTCGCCG GCCGACGTGC TGATGACGGT CGACGTCGGC AACCTGCTCG ACCTCGTCGA CGGCGGCGTC ACGCAGCCGG TGCGCTCGAA GGCACTCGAC GACGCGATTC CCGCGAACCT GCGCGCCACG AACGGCGACT GGTACGCGCT GTCGCTGCGC GACCGCGTGC TGTACGTCGA AAAAGACCTG AAGGTCGATG CGTTCCGTTA CGAGGATCTC GCCGATCCGA AATGGAAGGG CAAGGTCTGC ATCCGCTCGG GCCAGCATCC GTACAACACC GCGCTCGTCG CGGCGATGAT CGCGCACGAC GGCGAGGCCG CAACCGAGAC ATGGCTGCGC GGCGTGAAGG CGAACCTGGC GCGCAAGGCG ACCGGCGGCG ACCGCGACGT CGCGCGCGAC ATCCTCGGCG GAATCTGCGA CGTCGGCCTC GCGAACGCTT ACTACGTCGG CCACATGAAG CACGCGGAAC CGGGCAGCGA TGCGCGCAAA TGGGGCGACG CGATCAAGGT CGTGCGCCCG ACCTTCGCGA ACGCGAAGAG CGGCGGCACG CACGTGAACA TCAGCGGCGC GACGGTGGCG AAGCATGCGC CGCACAAGGC CAACGCGGTG AAACTGCTCG AATACCTGGT GTCGCCGGAA GCGCAGGCGC TGTATGCGCA GGCGAACTAC GAATACCCGG TGCGCGCGAA CGTGAAGCTC GACCCGGTGA TCGCGAGCTT CGGCACGCTG AAGGTCGATC CGCTGCCGCT GGCGGATATC GCGAAGCATC GCAAGCAGGC AAGCCAGCTC GTCGACAAGG TCGGCTTTGA CAACTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT