BuceA.00471.a
Putative fumarylpyruvate hydrolase
| CENTER ID: | BuceA.00471.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00471.a.B1.GE36625 | full length | 1 | 232 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00471.a.B1.PS01827 | full length | 1 | 232 |
Structures
4MAQ
DEPOSITED: 8/16/2013
DETERMINATION: XRay
CLONE: BuceA.00471.a.B1.GE36625
PROTEIN: BuceA.00471.a.B1.PS01827
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| PATRIC ID: | fig|216591.5.peg.5468 |
| RefSeq: | YP_002234304.1 |
| UniProt: | B4EKU2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAYVIDAAE RPSVEVDQSS ARFPVRRVFC VGRNYADHAR EMGADPDREP PFFFTKPADA IVPASGTVAY PPLTNDLHHE IELVVAIGKD GRSIDPADAL SHVWGYGVGV DLTRRDLQAE AKKLSRPWDW AKGFDASGPV TALRAATATG HPAAGRIWLA VNGDTRQQGD LADMIWPVPD VIAYVSRSVE LKAGDLIFTG TPAGVGALQP GDRVTGGVDG IATFEFVVGA KP
NT Sequence
atgtccgcct atgtcatcga cgccgccgag cgtccttccg tcgaagtcga ccagtcgtcc gcgcgctttc cggtgcgccg cgtcttctgc gtcggccgca actacgccga ccacgcccgc gaaatgggcg ccgaccccga ccgcgaaccg ccgttcttct tcaccaagcc cgccgacgcg atcgttccgg ccagcggcac ggtcgcctat ccgccgctga cgaacgatct ccatcatgaa atcgagctgg tcgtcgcgat cggcaaggac ggccggtcga tcgacccggc cgacgcgctg tcgcacgtgt ggggctacgg cgtcggcgtc gatctgacgc gccgcgacct gcaggccgaa gcgaagaagc tgagccggcc gtgggactgg gcgaaggggt tcgacgcgtc gggccccgta accgcgctgc gcgcggcgac ggccaccggc catccggccg ccggccgcat ctggctcgcg gtcaacggcg acacgcgcca gcaaggcgac ctggccgaca tgatctggcc cgtgcccgac gtgatcgcgt acgtgtcgcg ctcggtcgag ctgaaggccg gcgacctgat cttcaccggc acgccggccg gcgtcggcgc gctgcagccg ggcgatcgcg tgacgggcgg cgtcgacggc atcgcgacgt tcgaattcgt ggtcggcgcg aagccgtaa
Details for BuceA.00471.a.B1.GE36625
| HARVESTED ON: | 2/8/2013 |
| SEQUENCED ON: | 2/14/2013 |
| EXPECTED MW: | 26kDa |
| OBSERVED MW: | 27kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MSAYVIDAAE RPSVEVDQSS ARFPVRRVFC VGRNYADHAR EMGADPDREP PFFFTKPADA IVPASGTVAY PPLTNDLHHE IELVVAIGKD GRSIDPADAL SHVWGYGVGV DLTRRDLQAE AKKLSRPWDW AKGFDASGPV TALRAATATG HPAAGRIWLA VNGDTRQQGD LADMIWPVPD VIAYVSRSVE LKAGDLIFTG TPAGVGALQP GDRVTGGVDG IATFEFVVGA KP
Validated NT Sequence
atcttactca cttacggctt cgcgccgacc acgaattcga acgtcgcgat gccgtcgacg ccgcccgtca cgcgatcgcc cggctgcagc gcgccgacgc cggccggcgt gccggtgaag atcaggtcgc cggccttcag ctcgaccgag cgcgacacgt acgcgatcac gtcgggcacg ggccagatca tgtcggccag gtcgccttgc tggcgcgtgt cgccgttgac cgcgagccag atgcggccgg cggccggatg gccggtggcc gtcgccgcgc gcagcgcggt tacggggccc gacgcgtcga accccttcgc ccagtcccac ggccggctca gcttcttcgc ttcggcctgc aggtcgcggc gcgtcagatc gacgccgacg ccgtagcccc acacgtgcga cagcgcgtcg gccgggtcga tcgaccggcc gtccttgccg atcgcgacga ccagctcgat ttcatgatgg agatcgttcg tcagcggcgg ataggcgacc gtgccgctgg ccggaacgat cgcgtcggcg ggcttggtga agaagaacgg cggttcgcgg tcggggtcgg cgcccatttc gcgggcgtgg tcggcgtagt tgcggccgac gcagaagacg cggcgcaccg gaaagcgcgc ggacgactgg tcgacttcga cggaaggacg ctcggcggcg tcgatgacat aggcggacat atggtggtgg tggtggtgag cc
Expected Protein Sequence
MAHHHHHHMS AYVIDAAERP SVEVDQSSAR FPVRRVFCVG RNYADHAREM GADPDREPPF FFTKPADAIV PASGTVAYPP LTNDLHHEIE LVVAIGKDGR SIDPADALSH VWGYGVGVDL TRRDLQAEAK KLSRPWDWAK GFDASGPVTA LRAATATGHP AAGRIWLAVN GDTRQQGDLA DMIWPVPDVI AYVSRSVELK AGDLIFTGTP AGVGALQPGD RVTGGVDGIA TFEFVVGAKP
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg cctatgtcat cgacgccgcc gagcgtcctt ccgtcgaagt cgaccagtcg tccgcgcgct ttccggtgcg ccgcgtcttc tgcgtcggcc gcaactacgc cgaccacgcc cgcgaaatgg gcgccgaccc cgaccgcgaa ccgccgttct tcttcaccaa gcccgccgac gcgatcgttc cggccagcgg cacggtcgcc tatccgccgc tgacgaacga tctccatcat gaaatcgagc tggtcgtcgc gatcggcaag gacggccggt cgatcgaccc ggccgacgcg ctgtcgcacg tgtggggcta cggcgtcggc gtcgatctga cgcgccgcga cctgcaggcc gaagcgaaga agctgagccg gccgtgggac tgggcgaagg ggttcgacgc gtcgggcccc gtaaccgcgc tgcgcgcggc gacggccacc ggccatccgg ccgccggccg catctggctc gcggtcaacg gcgacacgcg ccagcaaggc gacctggccg acatgatctg gcccgtgccc gacgtgatcg cgtacgtgtc gcgctcggtc gagctgaagg ccggcgacct gatcttcacc ggcacgccgg ccggcgtcgg cgcgctgcag ccgggcgatc gcgtgacggg cggcgtcgac ggcatcgcga cgttcgaatt cgtggtcggc gcgaagccgt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for BuceA.00471.a.B1.PS01827
| PURIFICATION DATe: | 5/21/2013 |
| CONCENTRATION: | 60mg/ml |
| OBSERVED MW: | 26kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 96 |
Protocol Notes
notes unavailable
Validated AA Sequence
MSAYVIDAAE RPSVEVDQSS ARFPVRRVFC VGRNYADHAR EMGADPDREP PFFFTKPADA IVPASGTVAY PPLTNDLHHE IELVVAIGKD GRSIDPADAL SHVWGYGVGV DLTRRDLQAE AKKLSRPWDW AKGFDASGPV TALRAATATG HPAAGRIWLA VNGDTRQQGD LADMIWPVPD VIAYVSRSVE LKAGDLIFTG TPAGVGALQP GDRVTGGVDG IATFEFVVGA KP
Validated NT Sequence
atcttactca cttacggctt cgcgccgacc acgaattcga acgtcgcgat gccgtcgacg ccgcccgtca cgcgatcgcc cggctgcagc gcgccgacgc cggccggcgt gccggtgaag atcaggtcgc cggccttcag ctcgaccgag cgcgacacgt acgcgatcac gtcgggcacg ggccagatca tgtcggccag gtcgccttgc tggcgcgtgt cgccgttgac cgcgagccag atgcggccgg cggccggatg gccggtggcc gtcgccgcgc gcagcgcggt tacggggccc gacgcgtcga accccttcgc ccagtcccac ggccggctca gcttcttcgc ttcggcctgc aggtcgcggc gcgtcagatc gacgccgacg ccgtagcccc acacgtgcga cagcgcgtcg gccgggtcga tcgaccggcc gtccttgccg atcgcgacga ccagctcgat ttcatgatgg agatcgttcg tcagcggcgg ataggcgacc gtgccgctgg ccggaacgat cgcgtcggcg ggcttggtga agaagaacgg cggttcgcgg tcggggtcgg cgcccatttc gcgggcgtgg tcggcgtagt tgcggccgac gcagaagacg cggcgcaccg gaaagcgcgc ggacgactgg tcgacttcga cggaaggacg ctcggcggcg tcgatgacat aggcggacat atggtggtgg tggtggtgag cc
Expressed Protein Sequence
MAHHHHHHMS AYVIDAAERP SVEVDQSSAR FPVRRVFCVG RNYADHAREM GADPDREPPF FFTKPADAIV PASGTVAYPP LTNDLHHEIE LVVAIGKDGR SIDPADALSH VWGYGVGVDL TRRDLQAEAK KLSRPWDWAK GFDASGPVTA LRAATATGHP AAGRIWLAVN GDTRQQGDLA DMIWPVPDVI AYVSRSVELK AGDLIFTGTP AGVGALQPGD RVTGGVDGIA TFEFVVGAKP
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG CCTATGTCAT CGACGCCGCC GAGCGTCCTT CCGTCGAAGT CGACCAGTCG TCCGCGCGCT TTCCGGTGCG CCGCGTCTTC TGCGTCGGCC GCAACTACGC CGACCACGCC CGCGAAATGG GCGCCGACCC CGACCGCGAA CCGCCGTTCT TCTTCACCAA GCCCGCCGAC GCGATCGTTC CGGCCAGCGG CACGGTCGCC TATCCGCCGC TGACGAACGA TCTCCATCAT GAAATCGAGC TGGTCGTCGC GATCGGCAAG GACGGCCGGT CGATCGACCC GGCCGACGCG CTGTCGCACG TGTGGGGCTA CGGCGTCGGC GTCGATCTGA CGCGCCGCGA CCTGCAGGCC GAAGCGAAGA AGCTGAGCCG GCCGTGGGAC TGGGCGAAGG GGTTCGACGC GTCGGGCCCC GTAACCGCGC TGCGCGCGGC GACGGCCACC GGCCATCCGG CCGCCGGCCG CATCTGGCTC GCGGTCAACG GCGACACGCG CCAGCAAGGC GACCTGGCCG ACATGATCTG GCCCGTGCCC GACGTGATCG CGTACGTGTC GCGCTCGGTC GAGCTGAAGG CCGGCGACCT GATCTTCACC GGCACGCCGG CCGGCGTCGG CGCGCTGCAG CCGGGCGATC GCGTGACGGG CGGCGTCGAC GGCATCGCGA CGTTCGAATT CGTGGTCGGC GCGAAGCCGT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT