BuceA.00353.a
Maf-like protein BceJ2315_23540
| CENTER ID: | BuceA.00353.a |
| ORGANISM: | Burkholderia cenocepacia J2315 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 3 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00353.a.A1.GE33204 | full length | 1 | 215 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| BuceA.00353.a.A1.PS01814 | full length | 1 | 215 |
Structures
4OO0
DEPOSITED: 1/29/2014
DETERMINATION: XRay
CLONE: BuceA.00353.a.A1.GE33204
PROTEIN: BuceA.00353.a.A1.PS01814
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| OrthoMCL: | OG5_127554 |
| PATRIC ID: | fig|216591.5.peg.2500 |
| RefSeq: | YP_002231518.1 |
| UniProt: | B4E5S2 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MPSSTSPALF PTPLFQTLYL ASQSPRRQEL LQQIGVRFEL LLPRPDEDAE ALEAELPGEA ADAYVRRVTV AKAEAARARL VASGKPAAPV LVADTTVTID GAILGKPTDA DDALAMLTRL AGREHAVLTA VAVIDASGEL LPPALSRSSV RFAAASRDAY VRYVETGEPF GKAGAYAIQG RAAEFIERID GSHSGIMGLP LFETAALLRT ARVAF
NT Sequence
atgccgtcca gcacgtcccc cgcgcttttt ccgaccccgc ttttccagac gctttacctc gcttcgcaaa gcccgcgccg ccaggagctg ctgcagcaga tcggcgtacg cttcgagctg ctgctgccgc gccccgacga ggacgccgag gcgctcgagg ccgaactgcc cggcgaagcc gccgacgcgt acgtgcggcg cgtcaccgtc gcgaaggccg aggccgcgcg tgcccgcctc gtcgcgagcg gcaagccggc cgcgccggtg ctggtggccg atacgaccgt gacgatcgac ggcgcgatcc tcggcaagcc gaccgacgcc gacgatgcgc tcgcgatgct cacccgcctc gccggccgcg aacacgcggt gctgaccgcc gtcgccgtga tcgacgccag cggcgagctg ctgccgcccg cgctgtcgcg ctcgtcggtg cgcttcgcgg ccgcgtcgcg cgacgcatac gtgcgctacg tcgaaaccgg cgagccgttc ggcaaggccg gcgcgtacgc gatccagggc cgcgcggccg aattcatcga gcgaatcgac ggatcccatt caggtatcat gggtctgccc ctttttgaga ctgccgcgct gctgcgtacc gcacgcgtcg ccttctga
Details for BuceA.00353.a.A1.GE33204
| HARVESTED ON: | 8/30/2011 |
| SEQUENCED ON: | 9/2/2011 |
| EXPECTED MW: | 25kDa |
| OBSERVED MW: | 26kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Too many to count (100+) |
| TOTAL EXPRESSION LEVEL: | Low Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | pass with incomplete coverage |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPSSTSPAL FPTPLFQTLY LASQSPRRQE LLQQIGVRFE LLLPRPDEDA EALEAELPGE AADAYVRRVT VAKAEAARAR LVASGKPAAP VLVADTTVTI DGAILGKPTD ADDALAMLTR LAGREHAVLT AVAVIDASGE LLPPALSRSS VRFAAASRDA YVRYVETGEP FGKAGAYAIQ GRAAEFIERI DGSHSGIMGL PLFETAALLR TARVAF
Validated NT Sequence
tattagaagg cgacgcgtgc ggtacgcagc agcgcggcag tctcaaaaag gggcagaccc atgatacctg aatgggatcc gtcgattcgc tcgatgaatt cggccgcgcg gccctggatc gcgtacgcgc cggccttgcc gaacggctcg ccggtttcga cgtagcgcac gtatgcgtcg cgcgacgcgg ccgcgaagcg caccgacgag cgcgacagcg cgggcggcag cagctcgccg ctggcgtcga tcacggcgac ggcggtcagc accgcgtgtt cgcggccggc gaggcgggtg agcatcgcga gcgcatcgtc ggcgtcggtc ggcttgccga ggatcgcgcc gtcgatcgtc acggtcgtat cggccaccag caccggcgcg gccggcttgc cgctcgcgac gaggcgggca cgcgcggcct cggccttcgc gacggtgacg cgccgcacgt acgcgtcggc ggcttcgccg ggcagttcgg cctcgagcgc ctcggcgtcc tcgtcggggc gcggcagcag cagctcgaag cgtacgccga tctgctgcag cagctcctgg cggcgcgggc tttgcgaagc gaggtaaagc gtctggaaaa gcggggtcgg aaaaagcgcg ggggacgtgc tggacggcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSSTSPAL FPTPLFQTLY LASQSPRRQE LLQQIGVRFE LLLPRPDEDA EALEAELPGE AADAYVRRVT VAKAEAARAR LVASGKPAAP VLVADTTVTI DGAILGKPTD ADDALAMLTR LAGREHAVLT AVAVIDASGE LLPPALSRSS VRFAAASRDA YVRYVETGEP FGKAGAYAIQ GRAAEFIERI DGSHSGIMGL PLFETAALLR TARVAF
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gccgtccagc acgtcccccg cgctttttcc gaccccgctt ttccagacgc tttacctcgc ttcgcaaagc ccgcgccgcc aggagctgct gcagcagatc ggcgtacgct tcgagctgct gctgccgcgc cccgacgagg acgccgaggc gctcgaggcc gaactgcccg gcgaagccgc cgacgcgtac gtgcggcgcg tcaccgtcgc gaaggccgag gccgcgcgtg cccgcctcgt cgcgagcggc aagccggccg cgccggtgct ggtggccgat acgaccgtga cgatcgacgg cgcgatcctc ggcaagccga ccgacgccga cgatgcgctc gcgatgctca cccgcctcgc cggccgcgaa cacgcggtgc tgaccgccgt cgccgtgatc gacgccagcg gcgagctgct gccgcccgcg ctgtcgcgct cgtcggtgcg cttcgcggcc gcgtcgcgcg acgcatacgt gcgctacgtc gaaaccggcg agccgttcgg caaggccggc gcgtacgcga tccagggccg cgcggccgaa ttcatcgagc gaatcgacgg atcccattca ggtatcatgg gtctgcccct ttttgagact gccgcgctgc tgcgtaccgc acgcgtcgcc ttctaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa
Details for BuceA.00353.a.A1.PS01814
| PURIFICATION DATe: | 5/7/2013 |
| CONCENTRATION: | 45mg/ml |
| OBSERVED MW: | 27kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 9 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMPSSTSPAL FPTPLFQTLY LASQSPRRQE LLQQIGVRFE LLLPRPDEDA EALEAELPGE AADAYVRRVT VAKAEAARAR LVASGKPAAP VLVADTTVTI DGAILGKPTD ADDALAMLTR LAGREHAVLT AVAVIDASGE LLPPALSRSS VRFAAASRDA YVRYVETGEP FGKAGAYAIQ GRAAEFIERI DGSHSGIMGL PLFETAALLR TARVAF
Validated NT Sequence
tattagaagg cgacgcgtgc ggtacgcagc agcgcggcag tctcaaaaag gggcagaccc atgatacctg aatgggatcc gtcgattcgc tcgatgaatt cggccgcgcg gccctggatc gcgtacgcgc cggccttgcc gaacggctcg ccggtttcga cgtagcgcac gtatgcgtcg cgcgacgcgg ccgcgaagcg caccgacgag cgcgacagcg cgggcggcag cagctcgccg ctggcgtcga tcacggcgac ggcggtcagc accgcgtgtt cgcggccggc gaggcgggtg agcatcgcga gcgcatcgtc ggcgtcggtc ggcttgccga ggatcgcgcc gtcgatcgtc acggtcgtat cggccaccag caccggcgcg gccggcttgc cgctcgcgac gaggcgggca cgcgcggcct cggccttcgc gacggtgacg cgccgcacgt acgcgtcggc ggcttcgccg ggcagttcgg cctcgagcgc ctcggcgtcc tcgtcggggc gcggcagcag cagctcgaag cgtacgccga tctgctgcag cagctcctgg cggcgcgggc tttgcgaagc gaggtaaagc gtctggaaaa gcggggtcgg aaaaagcgcg ggggacgtgc tggacggcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc cat
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMPSSTSPAL FPTPLFQTLY LASQSPRRQE LLQQIGVRFE LLLPRPDEDA EALEAELPGE AADAYVRRVT VAKAEAARAR LVASGKPAAP VLVADTTVTI DGAILGKPTD ADDALAMLTR LAGREHAVLT AVAVIDASGE LLPPALSRSS VRFAAASRDA YVRYVETGEP FGKAGAYAIQ GRAAEFIERI DGSHSGIMGL PLFETAALLR TARVAF
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCCGTCCAGC ACGTCCCCCG CGCTTTTTCC GACCCCGCTT TTCCAGACGC TTTACCTCGC TTCGCAAAGC CCGCGCCGCC AGGAGCTGCT GCAGCAGATC GGCGTACGCT TCGAGCTGCT GCTGCCGCGC CCCGACGAGG ACGCCGAGGC GCTCGAGGCC GAACTGCCCG GCGAAGCCGC CGACGCGTAC GTGCGGCGCG TCACCGTCGC GAAGGCCGAG GCCGCGCGTG CCCGCCTCGT CGCGAGCGGC AAGCCGGCCG CGCCGGTGCT GGTGGCCGAT ACGACCGTGA CGATCGACGG CGCGATCCTC GGCAAGCCGA CCGACGCCGA CGATGCGCTC GCGATGCTCA CCCGCCTCGC CGGCCGCGAA CACGCGGTGC TGACCGCCGT CGCCGTGATC GACGCCAGCG GCGAGCTGCT GCCGCCCGCG CTGTCGCGCT CGTCGGTGCG CTTCGCGGCC GCGTCGCGCG ACGCATACGT GCGCTACGTC GAAACCGGCG AGCCGTTCGG CAAGGCCGGC GCGTACGCGA TCCAGGGCCG CGCGGCCGAA TTCATCGAGC GAATCGACGG ATCCCATTCA GGTATCATGG GTCTGCCCCT TTTTGAGACT GCCGCGCTGC TGCGTACCGC ACGCGTCGCC TTCTAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA