SSGCID: Target Page

BuceA.00262.a

GTP-binding protein Era 1 (GTP-binding protein Era 2)

CENTER ID:	BuceA.00262.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00262.a.B1.GE35016	full length		1	299

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.2968
RefSeq:	YP_002230160.1
UniProt:	B4EA47

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MNTPAPTGFR CGMIAIVGRP NVGKSTLMNA LVGQKISITS RKAQTTRHRI TGINTFDDAQ FVFVDTPGFQ TRHSTALNRS LNRAVTSTLT SVDVILFVIE AGRFGPDDQK VLDLIPPGMP TLLIANKIDR VNDKASLFPF MQKVSELREF TELVPLSAQK PEDIKRLLDT IKPYLPEGEP IYGEDELTDR SSRFLAAEIL REKVFRWTGD ELPYTSTVLI DKFEEEGRLK RIFATILVER DSHKAMVIGK KGEKLKQIST EARMDMEKLF DGPVYLETFV KVKSGWADNE AGLRAYGYE

NT Sequence

atgaacactc ccgctcctac cggtttccgt tgcggcatga tcgcgatcgt gggccgcccg aacgtcggca agtcgacgtt gatgaacgca ctcgtcggcc agaagatcag catcacgtcg cgcaaggcgc agacgacccg ccaccgcatc accggcatca acacgttcga cgacgcgcaa ttcgtattcg tcgacacgcc gggctttcag acccgtcaca gcaccgcgct gaaccgttcg ctgaaccgcg cggtcacgtc gacgctgacg tcggtcgacg tgatcctgtt cgtgatcgag gccggccgct tcggccccga cgaccagaag gtgctcgacc tgatcccgcc cggcatgccg acgctgctga tcgcgaacaa gatcgatcgc gtgaacgaca aggcgtcgct gttcccgttc atgcagaagg tgagcgaact gcgcgaattc accgagctcg tgccgctgtc ggcgcagaag ccggaagaca tcaagcgcct gctggacacg atcaagccgt acctgccgga aggcgagccg atctacggcg aggacgagct gaccgaccgc agctcgcgtt ttctcgcggc cgaaatcctg cgcgagaagg tgttccgctg gaccggcgac gagctgccgt acacgagcac cgtgctgatc gacaagttcg aggaggaggg gcgcctgaag cgcatcttcg cgacgatcct cgtcgagcgc gattcgcaca aggcgatggt gatcggcaag aagggcgaga agctcaagca gatcagcacc gaggcgcgga tggacatgga aaagctgttc gacggccccg tatacctcga gaccttcgtg aaggtgaaga gcggctgggc cgacaacgag gcggggctgc gtgcctatgg gtacgaatga

Details for BuceA.00262.a.B1.GE35016

HARVESTED ON:	3/19/2012
SEQUENCED ON:	3/22/2012
EXPECTED MW:	34kDa
OBSERVED MW:	34kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMN TPAPTGFRCG MIAIVGRPNV GKSTLMNALV GQKISITSRK AQTTRHRITG INTFDDAQFV FVDTPGFQTR HSTALNRSLN RAVTSTLTSV DVILFVIEAG RFGPDDQKVL DLIPPGMPTL LIANKIDRVN DKASLFPFMQ KVSELREFTE LVPLSAQKPE DIKRLLDTIK PYLPEGEPIY GEDELTDRSS RFLAAEILRE KVFRWTGDEL PYTSTVLIDK FEEEGRLKRI FATILVERDS HKAMVIGKKG EKLKQISTEA RMDMEKLFDG PVYLETFVKV KSGWADNEAG LRAYGYE

Validated NT Sequence

tactcactta ttcgtaccca taggcacgca gccccgcctc gttgtcggcc cagccgctct tcaccttcac gaaggtctcg aggtatacgg ggccgtcgaa cagcttttcc atgtccatcc gcgcctcggt gctgatctgc ttgagcttct cgcccttctt gccgatcacc atcgccttgt gcgaatcgcg ctcgacgagg atcgtcgcga agatgcgctt caggcgcccc tcctcctcga acttgtcgat cagcacggtg ctcgtgtacg gcagctcgtc gccggtccag cggaacacct tctcgcgcag gatttcggcc gcgagaaaac gcgagctgcg gtcggtcagc tcgtcctcgc cgtagatcgg ctcgccttcc ggcaggtacg gcttgatcgt gtccagcagg cgcttgatgt cttccggctt ctgcgccgac agcggcacga gctcggtgaa ttcgcgcagt tcgctcacct tctgcatgaa cgggaacagc gacgccttgt cgttcacgcg atcgatcttg ttcgcgatca gcagcgtcgg catgccgggc gggatcaggt cgagcacctt ctggtcgtcg gggccgaagc ggccggcctc gatcacgaac aggatcacgt cgaccgacgt cagcgtcgac gtgaccgcgc ggttcagcga acggttcagc gcggtgctgt gacgggtctg aaagcccggc gtgtcgacga atacgaattg cgcgtcgtcg aacgtgttga tgccggtgat gcggtggcgg gtcgtctgcg ccttgcgcga cgtgatgctg atcttctggc cgacgagtgc gttcatcaac gtcgacttgc cgacgttcgg gcggcccacg atcgcgatca tgccgcaacg gaaaccggta ggagcgggag tgttcatatg gtggtggtgg tggtgagcca t

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaca ctcccgctcc taccggtttc cgttgcggca tgatcgcgat cgtgggccgc ccgaacgtcg gcaagtcgac gttgatgaac gcactcgtcg gccagaagat cagcatcacg tcgcgcaagg cgcagacgac ccgccaccgc atcaccggca tcaacacgtt cgacgacgcg caattcgtat tcgtcgacac gccgggcttt cagacccgtc acagcaccgc gctgaaccgt tcgctgaacc gcgcggtcac gtcgacgctg acgtcggtcg acgtgatcct gttcgtgatc gaggccggcc gcttcggccc cgacgaccag aaggtgctcg acctgatccc gcccggcatg ccgacgctgc tgatcgcgaa caagatcgat cgcgtgaacg acaaggcgtc gctgttcccg ttcatgcaga aggtgagcga actgcgcgaa ttcaccgagc tcgtgccgct gtcggcgcag aagccggaag acatcaagcg cctgctggac acgatcaagc cgtacctgcc ggaaggcgag ccgatctacg gcgaggacga gctgaccgac cgcagctcgc gttttctcgc ggccgaaatc ctgcgcgaga aggtgttccg ctggaccggc gacgagctgc cgtacacgag caccgtgctg atcgacaagt tcgaggagga ggggcgcctg aagcgcatct tcgcgacgat cctcgtcgag cgcgattcgc acaaggcgat ggtgatcggc aagaagggcg agaagctcaa gcagatcagc accgaggcgc ggatggacat ggaaaagctg ttcgacggcc ccgtatacct cgagaccttc gtgaaggtga agagcggctg ggccgacaac gaggcggggc tgcgtgccta tgggtacgaa tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat