SSGCID: Target Page

BuceA.00185.a

Putative acyl-CoA dehydrogenase

CENTER ID:	BuceA.00185.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	crystallized
COMMUNITY REQUEST:	True
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00185.a.B1.GE36624	full length		1	596

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00185.a.B1.PS01796	full length		1	596

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.7318
RefSeq:	YP_002153992.1
UniProt:	B4EP04

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYNAPVKDM LFVLKELAGI DAVAQLPGFE DAGYDTAQAV LDESAKFCGE VLAPLNVEGD RNPSSWKDGV VTATPGFGEA FRQFVEGGWQ GLQHPAEYDG QGLPKLIATP CIEMLNASNL SFALCPLLTD GAIEALLTAG TDEQKQRYVP KLISGEWTGT MNLTEPQAGS DLALVRSRAE PQGDGTYKVF GTKIFITWGE HDMADNIVHL VLARTPNAPE GVKGISLFIV PKFMVNDDGS LGARNDVHCV SIEHKLGIKA SPTAVLQYGD HGGAIGYLVG EENRGLEYMF IMMNAARFGV GMQGIGVADR AYQKAAEFAK ERVQSRPVDG SAKQSVTIIH HPDVRRMLGT MRALTEGARA LAYVAAAHSD IAHRHSDEAT RARHQAIYEY LVPVVKGWST EMVNDVASLG VQVHGGMGFI EETGAAQYYR DARILAIYEG TTAIQANDLV GRKTLRDGGA VAKALLAEIG DTVTALGKLD GAAAASMKAQ LEKGARSLAA VVDYVLANVK QDPNAVFAGS VPYLKLAGVV LCGWQMARAL VAAHANRASD PAFFDAKIAI AQCYAEHVLV QAGALEASIV GAKGNESVLA LTEDQF

NT Sequence

atgagctata acgcccccgt caaggacatg ctgttcgtgc tgaaggaact cgcgggcatc gacgccgtcg cgcagttgcc gggcttcgag gatgcgggtt acgacacggc gcaggccgtg ctcgacgagt ccgcgaaatt ctgcggcgag gtactggcgc cgctgaacgt cgaaggcgac cgcaatccga gcagctggaa ggacggtgtc gtgaccgcga cgccgggctt cggcgaagcg ttccgccagt tcgtcgaagg cggctggcag gggctgcagc atccggccga gtacgacggc caggggctgc cgaagctgat cgcgacgccg tgcatcgaga tgctgaacgc gtcgaacctg tcgttcgcgc tgtgtccgct gctcaccgac ggcgcgatcg aggcgctgct gacggccggc accgacgagc agaagcagcg ctacgtgccg aagctgatct cgggcgaatg gaccggcacg atgaacctga ccgagccgca ggcgggctcc gacctcgcgc tggtgcgctc gcgcgccgag ccgcagggcg acggcaccta caaggtgttc ggcaccaaga tcttcatcac gtggggcgag cacgacatgg cggacaacat cgtccacctg gtgctcgcgc gcacgccgaa cgcgccggaa ggcgtgaagg gcatctcgct gttcatcgtg ccgaagttca tggtcaacga cgacggctcg ctcggtgcgc gcaacgacgt gcactgcgtg tcgatcgagc acaagctcgg gatcaaggcg agcccgacgg cggtgctgca gtatggcgat cacggcggcg cgatcggcta tctggtgggc gaggagaacc gcggcctcga atacatgttc atcatgatga acgcggcgcg cttcggggtc gggatgcagg ggatcggcgt ggccgaccgc gcgtaccaga aggcggccga attcgcgaag gaacgcgtgc aaagccgccc ggtggacggc tcggccaagc agtcggtgac gatcatccat cacccggacg tgcgccggat gctgggcacg atgcgtgcgc tgaccgaagg cgcgcgggcg ctggcctacg tggctgccgc gcacagcgac attgcccacc gccattcgga cgaggcgacg cgcgcgcgtc atcaggcaat ctacgagtat ctggtgccgg tcgtgaaggg ctggagcacg gagatggtga acgacgtcgc gagcctgggc gtgcaggtgc acggcgggat ggggttcatc gaggagacgg gcgcggcgca gtactaccgc gatgcgcgga tcctggcgat ctacgaaggg acgacggcga tccaggcgaa cgacctggtg ggccgcaaga cgctgcgcga cggcggcgcg gtggcgaagg cgctgctcgc ggaaatcggc gacacggtga cggcgctcgg caagctggac ggtgcggcgg ccgcgtcgat gaaggcgcag ctggagaagg gcgcgcgctc gctggcggcg gtggtggact acgtgctcgc gaacgtgaag caggacccga acgcggtgtt cgcgggcagc gtgccgtacc tgaagctggc gggcgtggtg ctgtgcggct ggcagatggc gcgcgcgctg gtggccgcgc acgcgaaccg tgcgagcgac ccggcgttct tcgacgcgaa gatcgcgatc gcgcaatgct atgcggagca cgtgctggta caggcgggtg cgctcgaagc gtcgatcgtc ggcgcgaagg gcaacgagag cgtgctcgcg ttgacggaag accagttctg a

Details for BuceA.00185.a.B1.GE36624

HARVESTED ON:	2/8/2013
SEQUENCED ON:	2/14/2013
EXPECTED MW:	64kDa
OBSERVED MW:	64kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	95

Validated AA Sequence

MLFVLKELAG IDAVAQLPGF EDAGYDTAQA VLDESAKFCG EVLAPLNVEG DRNPSSWKDG VVTATPGFGE AFRQFVEGGW QGLQHPAEYD GQGLPKLIAT PCIEMLNASN LSFALCPLLT DGAIEALLTA GTDEQKQRYV PKLISGEWTG TMNLTEPQAG SDLALVRSRA EPQGDGTYKV FGTKIFITWG EHDMADNIVH LVLARTPNAP EGVKGISLFI VPKFMVNDDX SLGARNDVHC VSIEHKLGXK ASPTAVLQYG DHGGAIGYLV GEENRGLXYX FIMMNAARFG VGMQXXXXXD RAYQKAXEFA KERVQSXPVD GSAKQSVTII HHPDVRRMLG TMRALTEGAR ALAYVAAAHS DIAHRHSDEA TRARHQAIYE YLVPVVKGWS TEMVNDVASL GVQVHGGMGF IEETGAAQYY RDARILAIYE GTTAIQANDL VGRKTLRDGG AVAKALLAEI GDTVTALGKL DGAAAASMKA QLEKGARSLA AVVXYVLANV KQDPNAVFAG SVPYLKLAGV VLCGWQMARA LVAAHANRAS DPAFFDAKIA IAQCYAEHVL VQAGALEASI VGAKGNESV

Validated NT Sequence

gcacgctctc gttgcccttc gcgccgacga tcgacgcttc gagcgcaccc gcctgtacca gcacgtgctc cgcatagcat tgcgcgatcg cgatcttcgc gtcgaagaac gccgggtcgc tcgcacggtt cgcgtgcgcg gccaccagcg cgcgcgccat ctgccagccg cacagcacca cgcccgccag cttcaggtac ggcacgctgc ccgcgaacac cgcgttcggg tcctgcttca cgttcgcgag cacgtantcc accaccgccg ccagcgagcg cgcgcccttc tccagctgcg ccttcatcga cgcggccgcc gcaccgtcca gcttgccgag cgccgtcacc gtgtcgccga tttccgcgag cagcgccttc gccaccgcgc cgccgtcgcg cagcgtcttg cggcccacca ggtcgttcgc ctggatcgcc gtcgtccctt cgtagatcgc caggatccgc gcatcgcggt agtactgcgc cgcgcccgtc tcctcgatga accccatccc gccgtgcacc tgcacgccca ggctcgcgac gtcgttcacc atctccgtgc tccagccctt cacgaccggn accagatact cgtagattgc ctgatgacgc gcgcgcgtcg cctcgtccga atggcggtgg gcaatgtcgc tgtgcgcggc agccacgtag gccagcgccc gcgcgccttc ggtcagcgca cgcatcgtgc ccagcatccg gcgcacgtcc gggtgatgga tgatcgtcac cgactgcttg gccgagccgt ccaccgggng gctttgcacg cgttccttcg cgaattcggn cgccttctgg tacgcgcggt cggncncgcn natccnctgc atcccgaccc cgaagcgcgc cgcgttcatc atgatgaaca ngtattngag gccgcggttc tcctcgccca ccagatagcc gatcgcgccg ccgtgatcgc catactgcag caccgccgtc gggctcgcct tgntcccgag cttgtgctcg atcgacacgc agtgcacgtc gttgcgcgca ccgagcgagc ngtcgtcgtt gaccatgaac ttcggcacga tgaacagcga gatgcccttc acgccttccg gcgcgttcgg cgtgcgcgcg agcaccaggt ggacgatgtt gtccgccatg tcgtgctcgc cccacgtgat gaagatcttg gtgccgaaca ccttgtaggt gccgtcgccc tgcggctcgg cgcgcgagcg caccagcgcg aggtcggagc ccgcctgcgg ctcggtcagg ttcatcgtgc cggtccattc gcccgagatc agcttcggca cgtagcgctg cttctgctcg tcggtgccgg ccgtcagcag cgcctcgatc gcgccgtcgg tgagcagcgg acacagcgcg aacgacaggt tcgacgcgtt cagcatctcg atgcacggcg tcgcgatcag cttcggcagc ccctggccgt cgtactcggc cggatgctgc agcccctgcc agccgccttc gacgaactgg cggaacgctt cgccgaagcc cggcgtcgcg gtcacgacac cgtccttcca gctgctcgga ttgcggtcgc cttcgacgtt cagcggcgcc agtacctcgc cgcagaattt cgcggactcg tcgagcacgg cctgcgccgt gtcgtaaccc gcatcctcga agcccggcaa ctgcgcgacg gcgtcgatgc ccgcgagttc cttcagcacg aacagcatgt ccttgacggg ggcgtta

Expected Protein Sequence

MAHHHHHHMS YNAPVKDMLF VLKELAGIDA VAQLPGFEDA GYDTAQAVLD ESAKFCGEVL APLNVEGDRN PSSWKDGVVT ATPGFGEAFR QFVEGGWQGL QHPAEYDGQG LPKLIATPCI EMLNASNLSF ALCPLLTDGA IEALLTAGTD EQKQRYVPKL ISGEWTGTMN LTEPQAGSDL ALVRSRAEPQ GDGTYKVFGT KIFITWGEHD MADNIVHLVL ARTPNAPEGV KGISLFIVPK FMVNDDGSLG ARNDVHCVSI EHKLGIKASP TAVLQYGDHG GAIGYLVGEE NRGLEYMFIM MNAARFGVGM QGIGVADRAY QKAAEFAKER VQSRPVDGSA KQSVTIIHHP DVRRMLGTMR ALTEGARALA YVAAAHSDIA HRHSDEATRA RHQAIYEYLV PVVKGWSTEM VNDVASLGVQ VHGGMGFIEE TGAAQYYRDA RILAIYEGTT AIQANDLVGR KTLRDGGAVA KALLAEIGDT VTALGKLDGA AAASMKAQLE KGARSLAAVV DYVLANVKQD PNAVFAGSVP YLKLAGVVLC GWQMARALVA AHANRASDPA FFDAKIAIAQ CYAEHVLVQA GALEASIVGA KGNESVLALT EDQF

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct ataacgcccc cgtcaaggac atgctgttcg tgctgaagga actcgcgggc atcgacgccg tcgcgcagtt gccgggcttc gaggatgcgg gttacgacac ggcgcaggcc gtgctcgacg agtccgcgaa attctgcggc gaggtactgg cgccgctgaa cgtcgaaggc gaccgcaatc cgagcagctg gaaggacggt gtcgtgaccg cgacgccggg cttcggcgaa gcgttccgcc agttcgtcga aggcggctgg caggggctgc agcatccggc cgagtacgac ggccaggggc tgccgaagct gatcgcgacg ccgtgcatcg agatgctgaa cgcgtcgaac ctgtcgttcg cgctgtgtcc gctgctcacc gacggcgcga tcgaggcgct gctgacggcc ggcaccgacg agcagaagca gcgctacgtg ccgaagctga tctcgggcga atggaccggc acgatgaacc tgaccgagcc gcaggcgggc tccgacctcg cgctggtgcg ctcgcgcgcc gagccgcagg gcgacggcac ctacaaggtg ttcggcacca agatcttcat cacgtggggc gagcacgaca tggcggacaa catcgtccac ctggtgctcg cgcgcacgcc gaacgcgccg gaaggcgtga agggcatctc gctgttcatc gtgccgaagt tcatggtcaa cgacgacggc tcgctcggtg cgcgcaacga cgtgcactgc gtgtcgatcg agcacaagct cgggatcaag gcgagcccga cggcggtgct gcagtatggc gatcacggcg gcgcgatcgg ctatctggtg ggcgaggaga accgcggcct cgaatacatg ttcatcatga tgaacgcggc gcgcttcggg gtcgggatgc aggggatcgg cgtggccgac cgcgcgtacc agaaggcggc cgaattcgcg aaggaacgcg tgcaaagccg cccggtggac ggctcggcca agcagtcggt gacgatcatc catcacccgg acgtgcgccg gatgctgggc acgatgcgtg cgctgaccga aggcgcgcgg gcgctggcct acgtggctgc cgcgcacagc gacattgccc accgccattc ggacgaggcg acgcgcgcgc gtcatcaggc aatctacgag tatctggtgc cggtcgtgaa gggctggagc acggagatgg tgaacgacgt cgcgagcctg ggcgtgcagg tgcacggcgg gatggggttc atcgaggaga cgggcgcggc gcagtactac cgcgatgcgc ggatcctggc gatctacgaa gggacgacgg cgatccaggc gaacgacctg gtgggccgca agacgctgcg cgacggcggc gcggtggcga aggcgctgct cgcggaaatc ggcgacacgg tgacggcgct cggcaagctg gacggtgcgg cggccgcgtc gatgaaggcg cagctggaga agggcgcgcg ctcgctggcg gcggtggtgg actacgtgct cgcgaacgtg aagcaggacc cgaacgcggt gttcgcgggc agcgtgccgt acctgaagct ggcgggcgtg gtgctgtgcg gctggcagat ggcgcgcgcg ctggtggccg cgcacgcgaa ccgtgcgagc gacccggcgt tcttcgacgc gaagatcgcg atcgcgcaat gctatgcgga gcacgtgctg gtacaggcgg gtgcgctcga agcgtcgatc gtcggcgcga agggcaacga gagcgtgctc gcgttgacgg aagaccagtt ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for BuceA.00185.a.B1.PS01796

PURIFICATION DATe:	4/9/2013
CONCENTRATION:	63mg/ml
OBSERVED MW:	65kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	97
PERCENT COVERAGE:	95

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCT ATAACGCCCC CGTCAAGGAC ATGCTGTTCG TGCTGAAGGA ACTCGCGGGC ATCGACGCCG TCGCGCAGTT GCCGGGCTTC GAGGATGCGG GTTACGACAC GGCGCAGGCC GTGCTCGACG AGTCCGCGAA ATTCTGCGGC GAGGTACTGG CGCCGCTGAA CGTCGAAGGC GACCGCAATC CGAGCAGCTG GAAGGACGGT GTCGTGACCG CGACGCCGGG CTTCGGCGAA GCGTTCCGCC AGTTCGTCGA AGGCGGCTGG CAGGGGCTGC AGCATCCGGC CGAGTACGAC GGCCAGGGGC TGCCGAAGCT GATCGCGACG CCGTGCATCG AGATGCTGAA CGCGTCGAAC CTGTCGTTCG CGCTGTGTCC GCTGCTCACC GACGGCGCGA TCGAGGCGCT GCTGACGGCC GGCACCGACG AGCAGAAGCA GCGCTACGTG CCGAAGCTGA TCTCGGGCGA ATGGACCGGC ACGATGAACC TGACCGAGCC GCAGGCGGGC TCCGACCTCG CGCTGGTGCG CTCGCGCGCC GAGCCGCAGG GCGACGGCAC CTACAAGGTG TTCGGCACCA AGATCTTCAT CACGTGGGGC GAGCACGACA TGGCGGACAA CATCGTCCAC CTGGTGCTCG CGCGCACGCC GAACGCGCCG GAAGGCGTGA AGGGCATCTC GCTGTTCATC GTGCCGAAGT TCATGGTCAA CGACGACGGC TCGCTCGGTG CGCGCAACGA CGTGCACTGC GTGTCGATCG AGCACAAGCT CGGGATCAAG GCGAGCCCGA CGGCGGTGCT GCAGTATGGC GATCACGGCG GCGCGATCGG CTATCTGGTG GGCGAGGAGA ACCGCGGCCT CGAATACATG TTCATCATGA TGAACGCGGC GCGCTTCGGG GTCGGGATGC AGGGGATCGG CGTGGCCGAC CGCGCGTACC AGAAGGCGGC CGAATTCGCG AAGGAACGCG TGCAAAGCCG CCCGGTGGAC GGCTCGGCCA AGCAGTCGGT GACGATCATC CATCACCCGG ACGTGCGCCG GATGCTGGGC ACGATGCGTG CGCTGACCGA AGGCGCGCGG GCGCTGGCCT ACGTGGCTGC CGCGCACAGC GACATTGCCC ACCGCCATTC GGACGAGGCG ACGCGCGCGC GTCATCAGGC AATCTACGAG TATCTGGTGC CGGTCGTGAA GGGCTGGAGC ACGGAGATGG TGAACGACGT CGCGAGCCTG GGCGTGCAGG TGCACGGCGG GATGGGGTTC ATCGAGGAGA CGGGCGCGGC GCAGTACTAC CGCGATGCGC GGATCCTGGC GATCTACGAA GGGACGACGG CGATCCAGGC GAACGACCTG GTGGGCCGCA AGACGCTGCG CGACGGCGGC GCGGTGGCGA AGGCGCTGCT CGCGGAAATC GGCGACACGG TGACGGCGCT CGGCAAGCTG GACGGTGCGG CGGCCGCGTC GATGAAGGCG CAGCTGGAGA AGGGCGCGCG CTCGCTGGCG GCGGTGGTGG ACTACGTGCT CGCGAACGTG AAGCAGGACC CGAACGCGGT GTTCGCGGGC AGCGTGCCGT ACCTGAAGCT GGCGGGCGTG GTGCTGTGCG GCTGGCAGAT GGCGCGCGCG CTGGTGGCCG CGCACGCGAA CCGTGCGAGC GACCCGGCGT TCTTCGACGC GAAGATCGCG ATCGCGCAAT GCTATGCGGA GCACGTGCTG GTACAGGCGG GTGCGCTCGA AGCGTCGATC GTCGGCGCGA AGGGCAACGA GAGCGTGCTC GCGTTGACGG AAGACCAGTT CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT