BuceA.00166.b

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase

CENTER ID: BuceA.00166.b
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: soluble
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00166.b.A1.GE33202 Full length( BuceA.00166.b ) 1 305
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3344
RefSeq: YP_002232330.1
UniProt: B4EDD8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSAPSGWATR QGTLARPLAI DGHGLHTGRR VGVRILPGRP EEGVTGIAFR RVAQGRTLAT LPVDPALRRA QPLCTMLRNA DGVGVRTIEH LLASLLACEI DHAIVEIDAE EVPILDGSAA PWVDAIRACG RVALDAPKRF IRVLRPVVVT DGEGDQRREM RIEPAPRYEL SVRNDLRGFG DMHWDGALTP AAFATEIAPS RSYGRVKWAV PAIVAGYLRG VPILRGARPS CTASIVGNRV LGGMRLPEEF VRHRVLDLVG DLALAGAPLL ARVSALRPSH EMNFRLVDAL LAESDAWQWA EFPDA
NT Sequence
ATGAGCGCGC CGTCCGGCTG GGCGACGCGT CAGGGCACGC TGGCACGTCC GCTGGCGATC GACGGTCACG GGCTGCACAC GGGGCGCCGG GTGGGTGTGC GGATCCTGCC CGGGCGTCCG GAAGAGGGCG TGACGGGCAT CGCGTTCCGT CGCGTCGCGC AGGGGCGCAC GCTCGCGACG CTGCCGGTCG ATCCCGCGCT GCGCCGCGCG CAGCCGCTGT GCACGATGCT GCGCAACGCC GACGGCGTCG GCGTTCGCAC GATCGAGCAT CTGCTTGCGT CACTGCTCGC ATGCGAGATC GATCACGCGA TCGTCGAGAT CGATGCGGAA GAAGTGCCGA TTCTCGATGG CAGCGCGGCA CCTTGGGTGG ATGCGATCCG CGCGTGCGGC CGTGTCGCGC TCGATGCGCC GAAACGCTTC ATCCGCGTGC TGCGGCCCGT CGTCGTCACG GACGGCGAGG GCGACCAGCG GCGCGAAATG CGGATCGAAC CGGCGCCGCG TTACGAACTG AGCGTGCGCA ACGACCTGCG CGGCTTCGGC GACATGCACT GGGATGGGGC GCTGACGCCG GCCGCATTCG CGACCGAAAT CGCGCCGTCG CGCTCGTATG GCCGCGTGAA GTGGGCGGTA CCCGCGATCG TCGCCGGTTA TCTGCGCGGC GTGCCGATCC TGCGCGGCGC GCGACCGTCG TGCACGGCGT CCATCGTCGG CAATCGCGTG CTGGGCGGGA TGCGGCTGCC GGAGGAGTTC GTCCGCCACC GCGTGCTCGA CCTGGTCGGC GATCTCGCGC TGGCCGGCGC GCCGCTGCTG GCGCGCGTGA GCGCACTGCG GCCGAGCCAC GAGATGAATT TCCGGCTGGT GGACGCGCTG CTGGCCGAAT CCGACGCATG GCAGTGGGCC GAGTTTCCCG ACGCG
Details for BuceA.00166.b.A1.GE33202
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPSGWAT RQGTLARPLA IDGHGLHTGR RVGVRILPGR PEEGVTGIAF RRVAQGRTLA TLPVDPALRR AQPLCTMLRN ADGVGVRTIE HLLASLLACE IDHAIVEIDA EEVPILDGSA APWVDAIRAC GRVALDAPKR FIRVLRPVVV TDGEGDQRRE MRIEPAPRYE LSVRNDLRGF GDMHWDGALT PAAFATEIAP SRSYGRVKWA VPAIVAGYLR GVPILRGARP SCTASIVGNR VLGGMRLPEE FVRHRVLDLV GDLALAGAPL LARVSALRPS HEMNFRLVDA LLAESDAWQW AEFP
Validated NT Sequence
cgggaaactc ggcccactgc catgcgtcgg attcggccag cagcgcgtcc accagccgga aattcatctc gtggctcggc cgcagtgcgc tcacgcgcgc cagcagcggc gcgccggcca gcgcgagatc gccgaccagg tcgagcacgc ggtggcggac gaactcctcc ggcagccgca tcccgcccag cacgcgattg ccgacgatgg acgccgtgca cgacggtcgc gcgccgcgca ggatcggcac gccgcgcaga taaccggcga cgatcgcggg taccgcccac ttcacgcggc catacgagcg cgacggcgcg atttcggtcg cgaatgcggc cggcgtcagc gccccatccc agtgcatgtc gccgaagccg cgcaggtcgt tgcgcacgct cagttcgtaa cgcggcgccg gttcgatccg catttcgcgc cgctggtcgc cctcgccgtc cgtgacgacg acgggccgca gcacgcggat gaagcgtttc ggcgcatcga gcgcgacacg gccgcacgcg cggatcgcat ccacccaagg tgccgcgctg ccatcgagaa tcggcacttc ttccgcatcg atctcgacga tcgcgtgatc gatctcgcat gcgagcagtg acgcaagcag atgctcgatc gtgcgaacgc cgacgccgtc ggcgttgcgc agcatcgtgc acagcggctg cgcgcggcgc agcgcgggat cgaccggcag cgtcgcgagc gtgcgcccct gcgcgacgcg acggaacgcg atgcccgtca cgccctcttc cggacgcccg ggcaggatcc gcacacccac ccggcgcccc gtgtgcagcc cgtgaccgtc gatcgccagc ggacgtgcca gcgtgccctg acgcgtcgcc cagccggacg gcgcgctcat cgaaccagga ccctgggtct gagcttccag ggtacccata tgatggtgat ggtgatgagc catggta
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSAPSGWAT RQGTLARPLA IDGHGLHTGR RVGVRILPGR PEEGVTGIAF RRVAQGRTLA TLPVDPALRR AQPLCTMLRN ADGVGVRTIE HLLASLLACE IDHAIVEIDA EEVPILDGSA APWVDAIRAC GRVALDAPKR FIRVLRPVVV TDGEGDQRRE MRIEPAPRYE LSVRNDLRGF GDMHWDGALT PAAFATEIAP SRSYGRVKWA VPAIVAGYLR GVPILRGARP SCTASIVGNR VLGGMRLPEE FVRHRVLDLV GDLALAGAPL LARVSALRPS HEMNFRLVDA LLAESDAWQW AEFPDA
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gagcgcgccg tccggctggg cgacgcgtca gggcacgctg gcacgtccgc tggcgatcga cggtcacggg ctgcacacgg ggcgccgggt gggtgtgcgg atcctgcccg ggcgtccgga agagggcgtg acgggcatcg cgttccgtcg cgtcgcgcag gggcgcacgc tcgcgacgct gccggtcgat cccgcgctgc gccgcgcgca gccgctgtgc acgatgctgc gcaacgccga cggcgtcggc gttcgcacga tcgagcatct gcttgcgtca ctgctcgcat gcgagatcga tcacgcgatc gtcgagatcg atgcggaaga agtgccgatt ctcgatggca gcgcggcacc ttgggtggat gcgatccgcg cgtgcggccg tgtcgcgctc gatgcgccga aacgcttcat ccgcgtgctg cggcccgtcg tcgtcacgga cggcgagggc gaccagcggc gcgaaatgcg gatcgaaccg gcgccgcgtt acgaactgag cgtgcgcaac gacctgcgcg gcttcggcga catgcactgg gatggggcgc tgacgccggc cgcattcgcg accgaaatcg cgccgtcgcg ctcgtatggc cgcgtgaagt gggcggtacc cgcgatcgtc gccggttatc tgcgcggcgt gccgatcctg cgcggcgcgc gaccgtcgtg cacggcgtcc atcgtcggca atcgcgtgct gggcgggatg cggctgccgg aggagttcgt ccgccaccgc gtgctcgacc tggtcggcga tctcgcgctg gccggcgcgc cgctgctggc gcgcgtgagc gcactgcggc cgagccacga gatgaatttc cggctggtgg acgcgctgct ggccgaatcc gacgcatggc agtgggccga gtttcccgac gcgtaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa