BuceA.00158.a

Glutamate racemase

CENTER ID: BuceA.00158.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00158.a.B1.GE37391 Full length( BuceA.00158.a ) 1 285
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00158.a.B1.PS02009 Full length( BuceA.00158.a ) 1 285

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2390
RefSeq: YP_002231414.1
UniProt: B4EEM8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTNPSDATAL RAAAPVGIFD SGLGGLSVLR AARAHLPDES FVYVADSHHA PYGPRDEAFI TERTLAIGEW LAREGAKALV IACNTATARA IAAIRERLSI PLVGVEPGIK PAAALSATGV AGVLATQSTL NSPRFQALLD RYGAGRRFIC QPGHGLVEAI ERGDTNSPAL RALLERYIQP MLDDGADTLV LGCTHYPFFT ETIRDLVGER LTIVDTSDAI ARQLARVLDE RGLRAPAGTH AAPPRFCSTS DGLQLRALAS TLLGLDAPVE SVTISSPNAR ACATA
NT Sequence
ATGACGAACC CGTCCGACGC TACCGCGCTG CGCGCCGCCG CCCCTGTCGG CATCTTCGAC TCGGGGCTCG GCGGCCTGTC GGTGCTGCGC GCCGCGCGCG CGCACCTGCC CGACGAATCG TTCGTCTACG TCGCCGACTC GCACCACGCG CCGTACGGCC CGCGCGACGA AGCCTTCATT ACGGAACGCA CGCTGGCCAT CGGCGAGTGG CTCGCCCGCG AAGGCGCGAA AGCGCTCGTC ATCGCATGCA ACACGGCAAC GGCCCGGGCG ATCGCGGCCA TCCGCGAACG CCTGTCGATC CCGCTCGTCG GTGTCGAGCC GGGCATCAAG CCGGCCGCCG CGCTGTCCGC GACCGGCGTC GCGGGCGTCC TCGCCACGCA ATCGACGTTG AACAGCCCGC GCTTCCAGGC GCTGCTCGAC CGCTACGGCG CCGGCCGCCG CTTCATCTGC CAACCCGGCC ACGGGCTCGT CGAAGCGATC GAACGCGGCG ACACGAACTC GCCCGCGTTG CGCGCGCTGC TCGAGCGTTA CATCCAGCCG ATGCTCGACG ACGGCGCCGA TACGCTGGTA CTCGGCTGCA CGCACTACCC GTTCTTCACA GAAACGATTC GTGACCTTGT TGGCGAGCGC CTGACGATCG TCGACACGAG CGACGCCATC GCCCGCCAGC TTGCGCGGGT CCTCGACGAA CGCGGGCTGC GCGCCCCGGC CGGCACGCAC GCCGCGCCGC CGCGTTTCTG CTCGACCAGC GACGGTCTGC AGCTACGCGC GCTCGCCTCG ACGCTGCTCG GCCTCGACGC GCCGGTCGAA TCCGTCACCA TTTCCTCGCC GAACGCGCGC GCCTGCGCGA CCGCC
Details for BuceA.00158.a.B1.GE37391
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Validated AA Sequence
MAHHHHHMTN PSDATALRAA APVGIFDSGL GGLSVLRAAR AHLPDESFVY VADSHHAPYG PRDEAFITER TLAIGEWLAR EGAKALVIAC NTATARAIAA IRERLSIPLV GVEPGIKPAA ALSATGVAGV LATQSTLNSP RFQALLDRYG AGRRFICQPG HGLVEAIERG DTNSPALRAL LERYIQPMLD DGADTLVLGC THYPFFTETI RDLVGERLTI VDTSDAIARQ LARVLDERGL RAPAGTHAAP PRFCSTSDGL QLRALASTLL GLDAPVESVT ISSPNARACA TA
Validated NT Sequence
tcttactcac ttaggcggtc gcgcaggcgc gcgcgttcgg cgaggaaatg gtgacggatt cgaccggcgc gtcgaggccg agcagcgtcg aggcgagcgc gcgtagctgc agaccgtcgc tggtcgagca gaaacgcggc ggcgcggcgt gcgtgccggc cggggcgcgc agcccgcgtt cgtcgaggac ccgcgcaagc tggcgggcga tggcgtcgct cgtgtcgacg atcgtcaggc gctcgccaac aaggtcacga atcgtttctg tgaagaacgg gtagtgcgtg cagccgagta ccagcgtatc ggcgccgtcg tcgagcatcg gctggatgta acgctcgagc agcgcgcgca acgcgggcga gttcgtgtcg ccgcgttcga tcgcttcgac gagcccgtgg ccgggttggc agatgaagcg gcggccggcg ccgtagcggt cgagcagcgc ctggaagcgc gggctgttca acgtcgattg cgtggcgagg acgcccgcga cgccggtcgc ggacagcgcg gcggccggct tgatgcccgg ctcgacaccg acgagcggga tcgacaggcg ttcgcggatg gccgcgatcg cccgggccgt tgccgtgttg catgcgatga cgagcgcttt cgcgccttcg cgggcgagcc actcgccgat ggccagcgtg cgttccgtaa tgaaggcttc gtcgcgcggg ccgtacggcg cgtggtgcga gtcggcgacg tagacgaacg attcgtcggg caggtgcgcg cgcgcggcgc gcagcaccga caggccgccg agccccgagt cgaagatgcc gacaggggcg gcggcgcgca gcgcggtagc gtcggacggg ttcgtcatat ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMT NPSDATALRA AAPVGIFDSG LGGLSVLRAA RAHLPDESFV YVADSHHAPY GPRDEAFITE RTLAIGEWLA REGAKALVIA CNTATARAIA AIRERLSIPL VGVEPGIKPA AALSATGVAG VLATQSTLNS PRFQALLDRY GAGRRFICQP GHGLVEAIER GDTNSPALRA LLERYIQPML DDGADTLVLG CTHYPFFTET IRDLVGERLT IVDTSDAIAR QLARVLDERG LRAPAGTHAA PPRFCSTSDG LQLRALASTL LGLDAPVESV TISSPNARAC ATA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga acccgtccga cgctaccgcg ctgcgcgccg ccgcccctgt cggcatcttc gactcggggc tcggcggcct gtcggtgctg cgcgccgcgc gcgcgcacct gcccgacgaa tcgttcgtct acgtcgccga ctcgcaccac gcgccgtacg gcccgcgcga cgaagccttc attacggaac gcacgctggc catcggcgag tggctcgccc gcgaaggcgc gaaagcgctc gtcatcgcat gcaacacggc aacggcccgg gcgatcgcgg ccatccgcga acgcctgtcg atcccgctcg tcggtgtcga gccgggcatc aagccggccg ccgcgctgtc cgcgaccggc gtcgcgggcg tcctcgccac gcaatcgacg ttgaacagcc cgcgcttcca ggcgctgctc gaccgctacg gcgccggccg ccgcttcatc tgccaacccg gccacgggct cgtcgaagcg atcgaacgcg gcgacacgaa ctcgcccgcg ttgcgcgcgc tgctcgagcg ttacatccag ccgatgctcg acgacggcgc cgatacgctg gtactcggct gcacgcacta cccgttcttc acagaaacga ttcgtgacct tgttggcgag cgcctgacga tcgtcgacac gagcgacgcc atcgcccgcc agcttgcgcg ggtcctcgac gaacgcgggc tgcgcgcccc ggccggcacg cacgccgcgc cgccgcgttt ctgctcgacc agcgacggtc tgcagctacg cgcgctcgcc tcgacgctgc tcggcctcga cgcgccggtc gaatccgtca ccatttcctc gccgaacgcg cgcgcctgcg cgaccgcctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for BuceA.00158.a.B1.PS02009
PURIFICATION DATe: 3/5/2014
CONCENTRATION: 66.74mg/ml
OBSERVED MW: 30kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 3
VIAL VOLUME: 110µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 99
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHMTN PSDATALRAA APVGIFDSGL GGLSVLRAAR AHLPDESFVY VADSHHAPYG PRDEAFITER TLAIGEWLAR EGAKALVIAC NTATARAIAA IRERLSIPLV GVEPGIKPAA ALSATGVAGV LATQSTLNSP RFQALLDRYG AGRRFICQPG HGLVEAIERG DTNSPALRAL LERYIQPMLD DGADTLVLGC THYPFFTETI RDLVGERLTI VDTSDAIARQ LARVLDERGL RAPAGTHAAP PRFCSTSDGL QLRALASTLL GLDAPVESVT ISSPNARACA TA
Validated NT Sequence
tcttactcac ttaggcggtc gcgcaggcgc gcgcgttcgg cgaggaaatg gtgacggatt cgaccggcgc gtcgaggccg agcagcgtcg aggcgagcgc gcgtagctgc agaccgtcgc tggtcgagca gaaacgcggc ggcgcggcgt gcgtgccggc cggggcgcgc agcccgcgtt cgtcgaggac ccgcgcaagc tggcgggcga tggcgtcgct cgtgtcgacg atcgtcaggc gctcgccaac aaggtcacga atcgtttctg tgaagaacgg gtagtgcgtg cagccgagta ccagcgtatc ggcgccgtcg tcgagcatcg gctggatgta acgctcgagc agcgcgcgca acgcgggcga gttcgtgtcg ccgcgttcga tcgcttcgac gagcccgtgg ccgggttggc agatgaagcg gcggccggcg ccgtagcggt cgagcagcgc ctggaagcgc gggctgttca acgtcgattg cgtggcgagg acgcccgcga cgccggtcgc ggacagcgcg gcggccggct tgatgcccgg ctcgacaccg acgagcggga tcgacaggcg ttcgcggatg gccgcgatcg cccgggccgt tgccgtgttg catgcgatga cgagcgcttt cgcgccttcg cgggcgagcc actcgccgat ggccagcgtg cgttccgtaa tgaaggcttc gtcgcgcggg ccgtacggcg cgtggtgcga gtcggcgacg tagacgaacg attcgtcggg caggtgcgcg cgcgcggcgc gcagcaccga caggccgccg agccccgagt cgaagatgcc gacaggggcg gcggcgcgca gcgcggtagc gtcggacggg ttcgtcatat ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMT NPSDATALRA AAPVGIFDSG LGGLSVLRAA RAHLPDESFV YVADSHHAPY GPRDEAFITE RTLAIGEWLA REGAKALVIA CNTATARAIA AIRERLSIPL VGVEPGIKPA AALSATGVAG VLATQSTLNS PRFQALLDRY GAGRRFICQP GHGLVEAIER GDTNSPALRA LLERYIQPML DDGADTLVLG CTHYPFFTET IRDLVGERLT IVDTSDAIAR QLARVLDERG LRAPAGTHAA PPRFCSTSDG LQLRALASTL LGLDAPVESV TISSPNARAC ATA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGACGA ACCCGTCCGA CGCTACCGCG CTGCGCGCCG CCGCCCCTGT CGGCATCTTC GACTCGGGGC TCGGCGGCCT GTCGGTGCTG CGCGCCGCGC GCGCGCACCT GCCCGACGAA TCGTTCGTCT ACGTCGCCGA CTCGCACCAC GCGCCGTACG GCCCGCGCGA CGAAGCCTTC ATTACGGAAC GCACGCTGGC CATCGGCGAG TGGCTCGCCC GCGAAGGCGC GAAAGCGCTC GTCATCGCAT GCAACACGGC AACGGCCCGG GCGATCGCGG CCATCCGCGA ACGCCTGTCG ATCCCGCTCG TCGGTGTCGA GCCGGGCATC AAGCCGGCCG CCGCGCTGTC CGCGACCGGC GTCGCGGGCG TCCTCGCCAC GCAATCGACG TTGAACAGCC CGCGCTTCCA GGCGCTGCTC GACCGCTACG GCGCCGGCCG CCGCTTCATC TGCCAACCCG GCCACGGGCT CGTCGAAGCG ATCGAACGCG GCGACACGAA CTCGCCCGCG TTGCGCGCGC TGCTCGAGCG TTACATCCAG CCGATGCTCG ACGACGGCGC CGATACGCTG GTACTCGGCT GCACGCACTA CCCGTTCTTC ACAGAAACGA TTCGTGACCT TGTTGGCGAG CGCCTGACGA TCGTCGACAC GAGCGACGCC ATCGCCCGCC AGCTTGCGCG GGTCCTCGAC GAACGCGGGC TGCGCGCCCC GGCCGGCACG CACGCCGCGC CGCCGCGTTT CTGCTCGACC AGCGACGGTC TGCAGCTACG CGCGCTCGCC TCGACGCTGC TCGGCCTCGA CGCGCCGGTC GAATCCGTCA CCATTTCCTC GCCGAACGCG CGCGCCTGCG CGACCGCCTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT