BuceA.00154.a

Chorismate synthase

CENTER ID: BuceA.00154.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00154.a.B1.GE37390 Full length( BuceA.00154.a ) 1 366
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00154.a.B1.PS01948 Full length( BuceA.00154.a ) 1 366

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1524
RefSeq: YP_002230598.1
UniProt: B4E7H2

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSGNTLGTLF TVTTFGESHG PAIGCVIDGC PPGMGLTEAD IQVELDRRKP GTSRHVTQRQ EADEVEILSG VFEGVTTGTP IALLIRNTDQ RSKDYGNIVE TFRPGHADYT YWQKYGIRDY RGGGRSSARL TAPIVGAGAV AKKWLRERFG VEVRGYMSGL GEIDVPFVDW SHVHENPFFS PNAAVVPELE AYMDALRKEG DSIGARIDVV ASGVPVGWGE PVFDRLDADI AKAMMSINAV KGVEIGAGFD SVAQRGSVHG DELTPAGFVG NHAGGVLGGI STGQDITVSI AIKPTSSIRT PRRSITKSGE EATVETFGRH DPCVGIRATP IAESMLALVL IDHALRHRAQ CGDVETPTPK IAGSAT
NT Sequence
ATGTCCGGCA ATACCCTCGG CACGCTTTTC ACTGTCACGA CCTTCGGCGA ATCGCACGGT CCCGCGATCG GCTGCGTGAT CGACGGCTGC CCGCCGGGGA TGGGGCTGAC CGAAGCCGAC ATCCAGGTCG AGCTCGACCG CCGCAAGCCG GGCACGTCGC GGCACGTGAC GCAGCGGCAG GAGGCCGACG AGGTCGAGAT CCTGTCGGGC GTGTTCGAGG GCGTGACGAC CGGCACGCCG ATCGCGCTGC TGATCCGCAA CACCGACCAG CGCAGCAAGG ATTACGGCAA CATCGTCGAG ACGTTCCGTC CCGGCCACGC GGACTACACC TACTGGCAGA AATACGGCAT CCGCGACTAC CGCGGCGGCG GCCGCTCGTC CGCGCGCCTG ACCGCGCCGA TCGTCGGCGC CGGCGCGGTC GCGAAGAAGT GGCTGCGCGA GCGCTTCGGG GTCGAGGTGC GCGGCTACAT GAGCGGCCTC GGCGAAATCG ACGTGCCGTT CGTCGACTGG TCGCACGTGC ACGAGAACCC GTTCTTCTCG CCGAATGCAG CGGTCGTGCC GGAACTCGAA GCGTACATGG ATGCGCTGCG CAAGGAGGGC GATTCGATCG GCGCGCGCAT CGACGTCGTC GCGTCGGGCG TGCCGGTCGG CTGGGGCGAG CCGGTGTTCG ACCGGCTCGA TGCCGATATC GCGAAGGCGA TGATGAGCAT CAACGCGGTG AAGGGCGTCG AGATCGGCGC GGGCTTCGAC AGCGTCGCGC AGCGCGGCTC GGTGCACGGC GACGAACTGA CGCCGGCCGG TTTCGTCGGC AACCACGCGG GCGGTGTGCT CGGCGGGATT TCGACGGGGC AGGACATCAC CGTGTCGATC GCGATCAAGC CGACCTCGAG CATCCGCACG CCGCGCCGCT CGATCACGAA GTCCGGGGAA GAGGCGACGG TCGAAACCTT CGGTCGCCAC GATCCGTGCG TCGGCATTCG CGCGACGCCG ATCGCGGAAT CGATGCTCGC GCTGGTGCTG ATCGACCATG CGTTGCGCCA TCGTGCGCAG TGCGGCGACG TCGAGACGCC CACGCCGAAG ATCGCCGGCA GCGCGACC
Details for BuceA.00154.a.B1.GE37390
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 21
PERCENT COVERAGE: 91
Validated AA Sequence
MAQRMVDQHQ REHRFRDRRR ANADARIVAT EGFDRRLFPG LRDRAARRAD ARGRLDRDRH GDVLPRRNPA EHTARVVADE TGRRQFVAVH RAALRDAVEA RADLDALHRV DAHHRLRDIG IEPVEHRLAP ADRHARRDDV DARADRIALL AQRIHVRFEF RHDRCIRREE RVLVHVRPVD ERHVDFAEAA HVAAHLDPEA LAQPLLRDRA GADDRRGQAR GRAAAAAVVA DAVFLPVGVV RVAGTERLDD VAVILAALVG VADQQRDRRA GRHALEHARQ DLDLVGLLPL RHVPRRARLA AVELDLDVGF GQPHPRRAAV DHAADRGTVR FAEGRDSEKR AEGI
Validated NT Sequence
ggcgtgggcg tctcgacgtc gccgcactgc gcacgatggc gcaacgcatg gtcgatcagc accagcgcga gcatcgattc cgcgatcggc gtcgcgcgaa tgccgacgca cggatcgtgg cgaccgaagg tttcgaccgt cgcctcttcc ccggacttcg tgatcgagcg gcgcggcgtg cggatgctcg aggtcggctt gatcgcgatc gacacggtga tgtcctgccc cgtcgaaatc ccgccgagca caccgcccgc gtggttgccg acgaaaccgg ccggcgtcag ttcgtcgccg tgcaccgagc cgcgctgcgc gacgctgtcg aagcccgcgc cgatctcgac gcccttcacc gcgttgatgc tcatcatcgc cttcgcgata tcggcatcga gccggtcgaa caccggctcg ccccagccga ccggcacgcc cgacgcgacg acgtcgatgc gcgcgccgat cgaatcgccc tccttgcgca gcgcatccat gtacgcttcg agttccggca cgaccgctgc attcggcgag aagaacgggt tctcgtgcac gtgcgaccag tcgacgaacg gcacgtcgat ttcgccgagg ccgctcatgt agccgcgcac ctcgaccccg aagcgctcgc gcagccactt cttcgcgacc gcgccggcgc cgacgatcgg cgcggtcagg cgcgcggacg agcggccgcc gccgcggtag tcgcggatgc cgtatttctg ccagtaggtg tagtccgcgt ggccgggacg gaacgtctcg acgatgttgc cgtaatcctt gctgcgctgg tcggtgttgc ggatcagcag cgcgatcggc gtgccggtcg tcacgccctc gaacacgccc gacaggatct cgacctcgtc ggcctcctgc cgctgcgtca cgtgccgcga cgtgcccggc ttgcggcggt cgagctcgac ctggatgtcg gcttcggtca gccccatccc cggcgggcag ccgtcgatca cgcagccgat cgcgggaccg tgcgattcgc cgaaggtcgt gacagtgaaa agcgtgccga gggtattgc
Expected Protein Sequence
MAHHHHHHMS GNTLGTLFTV TTFGESHGPA IGCVIDGCPP GMGLTEADIQ VELDRRKPGT SRHVTQRQEA DEVEILSGVF EGVTTGTPIA LLIRNTDQRS KDYGNIVETF RPGHADYTYW QKYGIRDYRG GGRSSARLTA PIVGAGAVAK KWLRERFGVE VRGYMSGLGE IDVPFVDWSH VHENPFFSPN AAVVPELEAY MDALRKEGDS IGARIDVVAS GVPVGWGEPV FDRLDADIAK AMMSINAVKG VEIGAGFDSV AQRGSVHGDE LTPAGFVGNH AGGVLGGIST GQDITVSIAI KPTSSIRTPR RSITKSGEEA TVETFGRHDP CVGIRATPIA ESMLALVLID HALRHRAQCG DVETPTPKIA GSAT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtccg gcaataccct cggcacgctt ttcactgtca cgaccttcgg cgaatcgcac ggtcccgcga tcggctgcgt gatcgacggc tgcccgccgg ggatggggct gaccgaagcc gacatccagg tcgagctcga ccgccgcaag ccgggcacgt cgcggcacgt gacgcagcgg caggaggccg acgaggtcga gatcctgtcg ggcgtgttcg agggcgtgac gaccggcacg ccgatcgcgc tgctgatccg caacaccgac cagcgcagca aggattacgg caacatcgtc gagacgttcc gtcccggcca cgcggactac acctactggc agaaatacgg catccgcgac taccgcggcg gcggccgctc gtccgcgcgc ctgaccgcgc cgatcgtcgg cgccggcgcg gtcgcgaaga agtggctgcg cgagcgcttc ggggtcgagg tgcgcggcta catgagcggc ctcggcgaaa tcgacgtgcc gttcgtcgac tggtcgcacg tgcacgagaa cccgttcttc tcgccgaatg cagcggtcgt gccggaactc gaagcgtaca tggatgcgct gcgcaaggag ggcgattcga tcggcgcgcg catcgacgtc gtcgcgtcgg gcgtgccggt cggctggggc gagccggtgt tcgaccggct cgatgccgat atcgcgaagg cgatgatgag catcaacgcg gtgaagggcg tcgagatcgg cgcgggcttc gacagcgtcg cgcagcgcgg ctcggtgcac ggcgacgaac tgacgccggc cggtttcgtc ggcaaccacg cgggcggtgt gctcggcggg atttcgacgg ggcaggacat caccgtgtcg atcgcgatca agccgacctc gagcatccgc acgccgcgcc gctcgatcac gaagtccggg gaagaggcga cggtcgaaac cttcggtcgc cacgatccgt gcgtcggcat tcgcgcgacg ccgatcgcgg aatcgatgct cgcgctggtg ctgatcgacc atgcgttgcg ccatcgtgcg cagtgcggcg acgtcgagac gcccacgccg aagatcgccg gcagcgcgac ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for BuceA.00154.a.B1.PS01948
PURIFICATION DATe: 12/4/2013
CONCENTRATION: 37mg/ml
OBSERVED MW: 37kDa
EXPRESSION LEVEL: High Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 1
VIAL VOLUME: 110µl
PERCENT IDENTITY: 21
PERCENT COVERAGE: 91
Protocol Notes
notes unavailable
Validated AA Sequence
MAQRMVDQHQ REHRFRDRRR ANADARIVAT EGFDRRLFPG LRDRAARRAD ARGRLDRDRH GDVLPRRNPA EHTARVVADE TGRRQFVAVH RAALRDAVEA RADLDALHRV DAHHRLRDIG IEPVEHRLAP ADRHARRDDV DARADRIALL AQRIHVRFEF RHDRCIRREE RVLVHVRPVD ERHVDFAEAA HVAAHLDPEA LAQPLLRDRA GADDRRGQAR GRAAAAAVVA DAVFLPVGVV RVAGTERLDD VAVILAALVG VADQQRDRRA GRHALEHARQ DLDLVGLLPL RHVPRRARLA AVELDLDVGF GQPHPRRAAV DHAADRGTVR FAEGRDSEKR AEGI
Validated NT Sequence
ggcgtgggcg tctcgacgtc gccgcactgc gcacgatggc gcaacgcatg gtcgatcagc accagcgcga gcatcgattc cgcgatcggc gtcgcgcgaa tgccgacgca cggatcgtgg cgaccgaagg tttcgaccgt cgcctcttcc ccggacttcg tgatcgagcg gcgcggcgtg cggatgctcg aggtcggctt gatcgcgatc gacacggtga tgtcctgccc cgtcgaaatc ccgccgagca caccgcccgc gtggttgccg acgaaaccgg ccggcgtcag ttcgtcgccg tgcaccgagc cgcgctgcgc gacgctgtcg aagcccgcgc cgatctcgac gcccttcacc gcgttgatgc tcatcatcgc cttcgcgata tcggcatcga gccggtcgaa caccggctcg ccccagccga ccggcacgcc cgacgcgacg acgtcgatgc gcgcgccgat cgaatcgccc tccttgcgca gcgcatccat gtacgcttcg agttccggca cgaccgctgc attcggcgag aagaacgggt tctcgtgcac gtgcgaccag tcgacgaacg gcacgtcgat ttcgccgagg ccgctcatgt agccgcgcac ctcgaccccg aagcgctcgc gcagccactt cttcgcgacc gcgccggcgc cgacgatcgg cgcggtcagg cgcgcggacg agcggccgcc gccgcggtag tcgcggatgc cgtatttctg ccagtaggtg tagtccgcgt ggccgggacg gaacgtctcg acgatgttgc cgtaatcctt gctgcgctgg tcggtgttgc ggatcagcag cgcgatcggc gtgccggtcg tcacgccctc gaacacgccc gacaggatct cgacctcgtc ggcctcctgc cgctgcgtca cgtgccgcga cgtgcccggc ttgcggcggt cgagctcgac ctggatgtcg gcttcggtca gccccatccc cggcgggcag ccgtcgatca cgcagccgat cgcgggaccg tgcgattcgc cgaaggtcgt gacagtgaaa agcgtgccga gggtattgc
Expressed Protein Sequence
MAHHHHHHMS GNTLGTLFTV TTFGESHGPA IGCVIDGCPP GMGLTEADIQ VELDRRKPGT SRHVTQRQEA DEVEILSGVF EGVTTGTPIA LLIRNTDQRS KDYGNIVETF RPGHADYTYW QKYGIRDYRG GGRSSARLTA PIVGAGAVAK KWLRERFGVE VRGYMSGLGE IDVPFVDWSH VHENPFFSPN AAVVPELEAY MDALRKEGDS IGARIDVVAS GVPVGWGEPV FDRLDADIAK AMMSINAVKG VEIGAGFDSV AQRGSVHGDE LTPAGFVGNH AGGVLGGIST GQDITVSIAI KPTSSIRTPR RSITKSGEEA TVETFGRHDP CVGIRATPIA ESMLALVLID HALRHRAQCG DVETPTPKIA GSAT
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCG GCAATACCCT CGGCACGCTT TTCACTGTCA CGACCTTCGG CGAATCGCAC GGTCCCGCGA TCGGCTGCGT GATCGACGGC TGCCCGCCGG GGATGGGGCT GACCGAAGCC GACATCCAGG TCGAGCTCGA CCGCCGCAAG CCGGGCACGT CGCGGCACGT GACGCAGCGG CAGGAGGCCG ACGAGGTCGA GATCCTGTCG GGCGTGTTCG AGGGCGTGAC GACCGGCACG CCGATCGCGC TGCTGATCCG CAACACCGAC CAGCGCAGCA AGGATTACGG CAACATCGTC GAGACGTTCC GTCCCGGCCA CGCGGACTAC ACCTACTGGC AGAAATACGG CATCCGCGAC TACCGCGGCG GCGGCCGCTC GTCCGCGCGC CTGACCGCGC CGATCGTCGG CGCCGGCGCG GTCGCGAAGA AGTGGCTGCG CGAGCGCTTC GGGGTCGAGG TGCGCGGCTA CATGAGCGGC CTCGGCGAAA TCGACGTGCC GTTCGTCGAC TGGTCGCACG TGCACGAGAA CCCGTTCTTC TCGCCGAATG CAGCGGTCGT GCCGGAACTC GAAGCGTACA TGGATGCGCT GCGCAAGGAG GGCGATTCGA TCGGCGCGCG CATCGACGTC GTCGCGTCGG GCGTGCCGGT CGGCTGGGGC GAGCCGGTGT TCGACCGGCT CGATGCCGAT ATCGCGAAGG CGATGATGAG CATCAACGCG GTGAAGGGCG TCGAGATCGG CGCGGGCTTC GACAGCGTCG CGCAGCGCGG CTCGGTGCAC GGCGACGAAC TGACGCCGGC CGGTTTCGTC GGCAACCACG CGGGCGGTGT GCTCGGCGGG ATTTCGACGG GGCAGGACAT CACCGTGTCG ATCGCGATCA AGCCGACCTC GAGCATCCGC ACGCCGCGCC GCTCGATCAC GAAGTCCGGG GAAGAGGCGA CGGTCGAAAC CTTCGGTCGC CACGATCCGT GCGTCGGCAT TCGCGCGACG CCGATCGCGG AATCGATGCT CGCGCTGGTG CTGATCGACC ATGCGTTGCG CCATCGTGCG CAGTGCGGCG ACGTCGAGAC GCCCACGCCG AAGATCGCCG GCAGCGCGAC CTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT