SSGCID: Target Page

BuceA.00148.a

Tyrosine recombinase XerC

CENTER ID:	BuceA.00148.a
ORGANISM:	Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	3
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
BuceA.00148.a.B1.GE37387	Full length( BuceA.00148.a )		1	306

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|216591.5.peg.526
RefSeq:	YP_002229666.1
UniProt:	B4E7Z6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTDDPIAAYL SNLKHVRQLS EHTLRAYTHE LDELKKLAAG RPLDALTAAD MRGAVARAHA GGLSARSISH RLSAWRAFYR WLAQRIEMPA NPVAAVRAPK RPKTLPKALS VDDASALMDA PLAGTTEGIR DHAILELFYS SGLRLAELIG LDVTYTQADG YRSAGWLDLA EAEVTVRGKG NKERKVPVGR KAIDALNAWL AVRGEFVKHD PHPLFVSVRG NRMSPGVVRE RVKRAALAAG IPANVHPHVL RHSFATHVLQ SSGDLRAVQE LLGHASVAAT QVYTSLDFQH LAKIYDSAHP RAKKRD

NT Sequence

ATGACCGACG ATCCGATCGC CGCCTACCTG TCGAACCTGA AGCACGTCAG GCAGCTGTCG GAACACACGC TGCGCGCGTA CACGCACGAA CTCGACGAAC TGAAGAAGCT CGCGGCCGGC CGGCCGCTCG ACGCGCTGAC CGCCGCCGAC ATGCGCGGCG CGGTCGCCCG CGCGCATGCG GGCGGGCTGT CCGCGCGCTC GATCTCGCAC CGGCTCTCCG CGTGGCGCGC GTTCTACCGC TGGCTCGCGC AACGCATCGA GATGCCCGCG AACCCGGTGG CCGCGGTGCG CGCGCCGAAG CGCCCGAAAA CGCTGCCGAA GGCGCTGTCG GTCGACGACG CATCCGCGCT GATGGACGCG CCGCTCGCCG GCACGACCGA AGGCATCCGC GATCACGCGA TCCTCGAGCT GTTCTATTCG TCGGGGCTGC GCCTCGCCGA GCTGATCGGG CTGGACGTGA CGTACACGCA GGCCGACGGC TACCGCTCGG CCGGCTGGCT CGATCTCGCC GAAGCCGAAG TCACCGTGCG CGGCAAGGGC AACAAGGAGC GCAAGGTGCC GGTCGGCCGC AAGGCGATCG ACGCGCTGAA CGCGTGGCTC GCGGTGCGCG GCGAATTCGT GAAGCACGAT CCGCATCCGC TGTTCGTGTC GGTGCGCGGC AACCGGATGT CGCCGGGCGT CGTGCGCGAG CGCGTGAAGC GCGCGGCGCT CGCCGCCGGC ATCCCCGCCA ACGTCCACCC GCACGTGTTG CGCCACTCGT TCGCGACGCA CGTGCTGCAG TCGAGCGGCG ACCTGCGCGC GGTGCAGGAA CTGCTCGGCC ACGCGAGCGT CGCCGCGACG CAGGTCTATA CGTCGCTCGA CTTCCAGCAT CTCGCGAAGA TTTACGACAG CGCGCATCCG CGCGCGAAGA AGCGCGAC

Details for BuceA.00148.a.B1.GE37387

HARVESTED ON:	9/27/2013
SEQUENCED ON:	10/28/2013
EXPECTED MW:	34kDa
OBSERVED MW:	35kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMT DDPIAAYLSN LKHVRQLSEH TLRAYTHELD ELKKLAAGRP LDALTAADMR GAVARAHAGG LSARSISHRL SAWRAFYRWL AQRIEMPANP VAAVRAPKRP KTLPKALSVD DASALMDAPL AGTTEGIRDH AILELFYSSG LRLAELIGLD VTYTQADGYR SAGWLDLAEA EVTVRGKGNK ERKVPVGRKA IDALNAWLAV RGEFVKHDPH PLFVSVRGNR MSPGVVRERV KRAALAAGIP ANVHPHVLRH SFATHVLQSS GDLRAVQELL GHASVAATQV YTSLDFQHLA KIYDSAHPRA KKRD

Validated NT Sequence

atggctcacc accaccacca ccatatgacc gacgatccga tcgccgccta cctgtcgaac ctgaagcacg tcaggcagct gtcggaacac acgctgcgcg cgtacacgca cgaactcgac gaactgaaga agctcgcggc cggccggccg ctcgacgcgc tgaccgccgc cgacatgcgc ggcgcggtcg cccgcgcgca tgcgggcggg ctgtccgcgc gctcgatctc gcaccggctc tccgcgtggc gcgcgttcta ccgctggctc gcgcaacgca tcgagatgcc cgcgaacccg gtggccgcgg tgcgcgcgcc gaagcgcccg aaaacgctgc cgaaggcgct gtcggtcgac gacgcatccg cgctgatgga cgcgccgctc gccggcacga ccgaaggcat ccgcgatcac gcgatcctcg agctgttcta ttcgtcgggg ctgcgcctcg ccgagctgat cgggctggac gtgacgtaca cgcaggccga cggctaccgc tcggccggct ggctcgatct cgccgaagcc gaagtcaccg tgcgcggcaa gggcaacaag gagcgcaagg tgccggtcgg ccgcaaggcg atcgacgcgc tgaacgcgtg gctcgcggtg cgcggcgaat tcgtgaagca cgatccgcat ccgctgttcg tgtcggtgcg cggcaaccgg atgtcgccgg gcgtcgtgcg cgagcgcgtg aagcgcgcgg cgctcgccgc cggcatcccc gccaacgtcc acccgcacgt gttgcgccac tcgttcgcga cgcacgtgct gcagtcgagc ggcgacctgc gcgcggtgca ggaactgctc ggccacgcga gcgtcgccgc gacgcaggtc tatacgtcgc tcgacttcca gcatctcgcg aagatttacg acagcgcgca tccgcgcgcg aagaagcgcg actaagtgag taagat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaccg acgatccgat cgccgcctac ctgtcgaacc tgaagcacgt caggcagctg tcggaacaca cgctgcgcgc gtacacgcac gaactcgacg aactgaagaa gctcgcggcc ggccggccgc tcgacgcgct gaccgccgcc gacatgcgcg gcgcggtcgc ccgcgcgcat gcgggcgggc tgtccgcgcg ctcgatctcg caccggctct ccgcgtggcg cgcgttctac cgctggctcg cgcaacgcat cgagatgccc gcgaacccgg tggccgcggt gcgcgcgccg aagcgcccga aaacgctgcc gaaggcgctg tcggtcgacg acgcatccgc gctgatggac gcgccgctcg ccggcacgac cgaaggcatc cgcgatcacg cgatcctcga gctgttctat tcgtcggggc tgcgcctcgc cgagctgatc gggctggacg tgacgtacac gcaggccgac ggctaccgct cggccggctg gctcgatctc gccgaagccg aagtcaccgt gcgcggcaag ggcaacaagg agcgcaaggt gccggtcggc cgcaaggcga tcgacgcgct gaacgcgtgg ctcgcggtgc gcggcgaatt cgtgaagcac gatccgcatc cgctgttcgt gtcggtgcgc ggcaaccgga tgtcgccggg cgtcgtgcgc gagcgcgtga agcgcgcggc gctcgccgcc ggcatccccg ccaacgtcca cccgcacgtg ttgcgccact cgttcgcgac gcacgtgctg cagtcgagcg gcgacctgcg cgcggtgcag gaactgctcg gccacgcgag cgtcgccgcg acgcaggtct atacgtcgct cgacttccag catctcgcga agatttacga cagcgcgcat ccgcgcgcga agaagcgcga ctgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat