BuceA.00141.a

Putative malonyl CoA-acyl carrier protein transacylase

CENTER ID: BuceA.00141.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00141.a.B2.GE37382 Mature protein( BuceA.00141.a ) 14 310
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.1291
RefSeq: YP_002230384.1
UniProt: B4EEI3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTLAILCSGQ GAQRADMFDL TGSAPQADAL FAHAGRLLGD DPRTWVRQAG PDALRENRAA QILCTVQALA AAALLDAVWP RRRCVAGYSV GEVGSWSVAG MIGPHDALDL ADARARAMDA ASSGDERMVF VRGLTRVQLA QLCDGRDAAI AIANPGDAFV VAGRHVDVDA VADDAQRAGA LRVAPVCVRI ASHTRRLAAA VPAFRATLAA TRVRRPLPGT RLFSGIDGAS VLDVDTGLDK LARQIAEPVE WAACLAACVE AGATAFLELG PGRALAEMAG GAYPALPARS LADFRSVDGV TSWLARVAAG
NT Sequence
ATGACGCTGG CCATCCTGTG CTCGGGGCAA GGCGCGCAGC GCGCCGACAT GTTCGACCTC ACCGGGTCGG CGCCGCAGGC CGATGCACTG TTCGCGCATG CGGGCCGGCT GCTCGGCGAC GATCCGCGCA CGTGGGTCCG GCAGGCCGGG CCCGATGCGC TGCGGGAAAA CCGTGCAGCG CAGATCCTCT GCACGGTCCA GGCGCTCGCG GCGGCCGCGC TGCTCGACGC GGTGTGGCCG CGCCGACGCT GTGTCGCCGG CTACAGCGTC GGCGAGGTCG GCTCGTGGAG CGTCGCGGGG ATGATCGGGC CGCACGACGC GCTCGACCTG GCCGATGCGC GGGCGCGCGC GATGGATGCC GCGAGCAGCG GCGACGAGCG GATGGTGTTC GTGCGCGGCC TGACGCGCGT GCAACTGGCG CAGTTGTGCG ACGGTCGCGA CGCGGCCATC GCGATCGCGA ACCCCGGCGA TGCGTTCGTG GTCGCCGGGC GGCATGTCGA TGTCGATGCC GTGGCCGACG ACGCGCAGCG CGCCGGCGCG CTGCGCGTCG CGCCCGTCTG CGTGCGGATC GCGTCGCACA CGCGTCGGCT GGCCGCCGCC GTGCCGGCAT TCCGCGCGAC GCTGGCCGCG ACGCGCGTGC GCCGGCCGCT GCCCGGCACG CGGCTGTTCT CGGGAATCGA CGGCGCGTCG GTGCTCGATG TCGATACGGG CCTCGACAAG CTCGCGCGGC AGATCGCGGA GCCGGTCGAG TGGGCAGCCT GCCTCGCCGC GTGCGTGGAG GCCGGCGCCA CCGCGTTTCT CGAACTCGGC CCGGGGCGCG CGCTGGCCGA GATGGCGGGC GGCGCGTATC CCGCGTTGCC GGCGCGCAGC CTCGCGGATT TCCGTTCGGT CGACGGCGTG ACGAGTTGGC TCGCGCGCGT CGCAGCCGGC
Details for BuceA.00141.a.B2.GE37382
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with sequence variation
PERCENT IDENTITY: 98
PERCENT COVERAGE: 93
Validated AA Sequence
MAHHHHHHMR ADMFDLTGSA PQADALFAHA GRLLGDDPRT WVRQAGPDAL RENRAAQILC TVQALAAAAL LDAVWPRRRC VAGYSVGEVG SWSVAGMIGP HDALDLADAR ARAMDAASSG DERMVFVRGL TRVQLAQLCD GRDAAIAIAN PGDAFVVAGR HVDVDAVADD AQRAGALRVA PVCVRIASHT RRLAAAVPAF RATLAATRVR RPLPGTRLFS GIDGASVLDV DTGLDKLARQ IAXPVEWAAC LAACVEAGAT AFLELGPGRA LAXMAGGAYP ALXAR
Validated NT Sequence
atggctcacc accaccacca ccatatgcgc gccgacatgt tcgacctcac cgggtcggcg ccgcaggccg atgcactgtt cgcgcatgcg ggccggctgc tcggcgacga tccgcgcacg tgggtccggc aggccgggcc cgatgcgctg cgggaaaacc gtgcagcgca gatcctctgc acggtccagg cgctcgcggc ggccgcgctg ctcgacgcgg tgtggccgcg ccgacgctgt gtcgccggct acagcgtcgg cgaggtcggc tcgtggagcg tcgcggggat gatcgggccg cacgacgcgc tcgacctggc cgatgcgcgg gcgcgcgcga tggatgccgc gagcagcggc gacgagcgga tggtgttcgt gcgcggcctg acgcgcgtgc aactggcgca gttgtgcgac ggtcgcgacg cggccatcgc gatcgcgaac cccggcgatg cgttcgtggt cgccgggcgg catgtcgatg tcgatgccgt ggccgacgac gcgcagcgcg ccggcgcgct gcgcgtcgcg cccgtctgcg tgcggatcgc gtcgcacacg cgtcggctgg ccgccgccgt gccggcattc cgcgcgacgc tggccgcgac gcgcgtgcgc cggccgctgc ccggcacgcg gctgttctcg ggaatcgacg gcgcgtcggt gctcgatgtc gatacgggcc tcgacaagct cgcgcggcag atcgcgnanc cggtcgagtg ggcagcctgc ctcgccgcgt gcgtggaggc cggcgccacc gcgtttctcg aactcggccc ggggcgcgcg ctggccgana tggcgggcgg cgcgtatccc gcgttgcngg cgcgca
Expected Protein Sequence
MAHHHHHHRA DMFDLTGSAP QADALFAHAG RLLGDDPRTW VRQAGPDALR ENRAAQILCT VQALAAAALL DAVWPRRRCV AGYSVGEVGS WSVAGMIGPH DALDLADARA RAMDAASSGD ERMVFVRGLT RVQLAQLCDG RDAAIAIANP GDAFVVAGRH VDVDAVADDA QRAGALRVAP VCVRIASHTR RLAAAVPAFR ATLAATRVRR PLPGTRLFSG IDGASVLDVD TGLDKLARQI AEPVEWAACL AACVEAGATA FLELGPGRAL AEMAGGAYPA LPARSLADFR SVDGVTSWLA RVAAG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcgcgccg acatgttcga cctcaccggg tcggcgccgc aggccgatgc actgttcgcg catgcgggcc ggctgctcgg cgacgatccg cgcacgtggg tccggcaggc cgggcccgat gcgctgcggg aaaaccgtgc agcgcagatc ctctgcacgg tccaggcgct cgcggcggcc gcgctgctcg acgcggtgtg gccgcgccga cgctgtgtcg ccggctacag cgtcggcgag gtcggctcgt ggagcgtcgc ggggatgatc gggccgcacg acgcgctcga cctggccgat gcgcgggcgc gcgcgatgga tgccgcgagc agcggcgacg agcggatggt gttcgtgcgc ggcctgacgc gcgtgcaact ggcgcagttg tgcgacggtc gcgacgcggc catcgcgatc gcgaaccccg gcgatgcgtt cgtggtcgcc gggcggcatg tcgatgtcga tgccgtggcc gacgacgcgc agcgcgccgg cgcgctgcgc gtcgcgcccg tctgcgtgcg gatcgcgtcg cacacgcgtc ggctggccgc cgccgtgccg gcattccgcg cgacgctggc cgcgacgcgc gtgcgccggc cgctgcccgg cacgcggctg ttctcgggaa tcgacggcgc gtcggtgctc gatgtcgata cgggcctcga caagctcgcg cggcagatcg cggagccggt cgagtgggca gcctgcctcg ccgcgtgcgt ggaggccggc gccaccgcgt ttctcgaact cggcccgggg cgcgcgctgg ccgagatggc gggcggcgcg tatcccgcgt tgccggcgcg cagcctcgcg gatttccgtt cggtcgacgg cgtgacgagt tggctcgcgc gcgtcgcagc cggctgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat