BuceA.00138.a

Putative alanyl-tRNA synthetase

CENTER ID: BuceA.00138.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00138.a.B1.GE37381 Full length( BuceA.00138.a ) 1 243
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.2475
RefSeq: YP_002231496.1
UniProt: B4E5Q0

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTQALFRED AYLTQCEAIV QAVGDDGIRL DRTVFYPLGG GQAGDTGTLT LPDGSTIAIA DTRKAKFDGA TPDDAVHVPA PDQEASVATL APGTRVVAAI DWLRRYRHMR LHTAAHLMCA VLPYAVDGCS VTADYVRLDF ATSDAIDRDD VERRLAGFVV GAHPVTTEWI TDDEMAERPE LVRTMSVKPP MGLGRVRLLR IENVDLQPCG GTHVRNTSEI GGLRVAKLEK KSARTRRLVL EFA
NT Sequence
ATGACGACAC AAGCGCTGTT TCGCGAAGAC GCGTACCTCA CGCAATGCGA GGCGATCGTC CAGGCCGTCG GCGACGACGG CATCCGGCTC GACCGCACGG TGTTCTATCC GCTCGGCGGC GGCCAGGCCG GCGACACCGG CACGCTGACG CTGCCCGACG GCAGCACGAT CGCGATCGCC GACACGCGCA AGGCGAAGTT CGACGGTGCG ACGCCCGACG ACGCCGTGCA CGTGCCCGCG CCGGACCAGG AGGCGAGCGT CGCGACGCTC GCCCCCGGCA CGCGCGTGGT CGCGGCGATC GACTGGCTGC GCCGCTACCG GCACATGCGC CTGCATACGG CCGCGCACCT GATGTGCGCG GTGCTGCCGT ATGCGGTCGA CGGCTGCAGC GTGACGGCCG ACTACGTGCG GCTCGATTTC GCGACGTCCG ACGCGATCGA CCGCGACGAC GTCGAGCGGC GCCTCGCGGG CTTCGTCGTC GGCGCGCATC CGGTCACGAC CGAATGGATC ACCGACGACG AGATGGCCGA GCGGCCCGAA CTCGTGCGCA CGATGAGCGT GAAGCCGCCG ATGGGCCTCG GCCGCGTGCG GCTGCTGCGC ATCGAAAACG TCGACCTGCA GCCGTGCGGC GGCACGCATG TGCGCAACAC GTCCGAAATC GGTGGGCTGC GGGTCGCGAA ACTGGAAAAG AAGAGTGCGC GCACACGGCG CCTCGTACTG GAGTTTGCA
Details for BuceA.00138.a.B1.GE37381
HARVESTED ON: 9/27/2013
SEQUENCED ON: 10/3/2013
EXPECTED MW: 27kDa
OBSERVED MW: 27kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMT TQALFREDAY LTQCEAIVQA VGDDGIRLDR TVFYPLGGGQ AGDTGTLTLP DGSTIAIADT RKAKFDGATP DDAVHVPAPD QEASVATLAP GTRVVAAIDW LRRYRHMRLH TAAHLMCAVL PYAVDGCSVT ADYVRLDFAT SDAIDRDDVE RRLAGFVVGA HPVTTEWITD DEMAERPELV RTMSVKPPMG LGRVRLLRIE NVDLQPCGGT HVRNTSEIGG LRVAKLEKKS ARTRRLVLEF A
Validated NT Sequence
atcttactca cttatgcaaa ctccagtacg aggcgccgtg tgcgcgcact cttcttttcc agtttcgcga cccgcagccc accgatttcg gacgtgttgc gcacatgcgt gccgccgcac ggctgcaggt cgacgttttc gatgcgcagc agccgcacgc ggccgaggcc catcggcggc ttcacgctca tcgtgcgcac gagttcgggc cgctcggcca tctcgtcgtc ggtgatccat tcggtcgtga ccggatgcgc gccgacgacg aagcccgcga ggcgccgctc gacgtcgtcg cggtcgatcg cgtcggacgt cgcgaaatcg agccgcacgt agtcggccgt cacgctgcag ccgtcgaccg catacggcag caccgcgcac atcaggtgcg cggccgtatg caggcgcatg tgccggtagc ggcgcagcca gtcgatcgcc gcgaccacgc gcgtgccggg ggcgagcgtc gcgacgctcg cctcctggtc cggcgcgggc acgtgcacgg cgtcgtcggg cgtcgcaccg tcgaacttcg ccttgcgcgt gtcggcgatc gcgatcgtgc tgccgtcggg cagcgtcagc gtgccggtgt cgccggcctg gccgccgccg agcggataga acaccgtgcg gtcgagccgg atgccgtcgt cgccgacggc ctggacgatc gcctcgcatt gcgtgaggta cgcgtcttcg cgaaacagcg cttgtgtcgt catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHMT TQALFREDAY LTQCEAIVQA VGDDGIRLDR TVFYPLGGGQ AGDTGTLTLP DGSTIAIADT RKAKFDGATP DDAVHVPAPD QEASVATLAP GTRVVAAIDW LRRYRHMRLH TAAHLMCAVL PYAVDGCSVT ADYVRLDFAT SDAIDRDDVE RRLAGFVVGA HPVTTEWITD DEMAERPELV RTMSVKPPMG LGRVRLLRIE NVDLQPCGGT HVRNTSEIGG LRVAKLEKKS ARTRRLVLEF A
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacga cacaagcgct gtttcgcgaa gacgcgtacc tcacgcaatg cgaggcgatc gtccaggccg tcggcgacga cggcatccgg ctcgaccgca cggtgttcta tccgctcggc ggcggccagg ccggcgacac cggcacgctg acgctgcccg acggcagcac gatcgcgatc gccgacacgc gcaaggcgaa gttcgacggt gcgacgcccg acgacgccgt gcacgtgccc gcgccggacc aggaggcgag cgtcgcgacg ctcgcccccg gcacgcgcgt ggtcgcggcg atcgactggc tgcgccgcta ccggcacatg cgcctgcata cggccgcgca cctgatgtgc gcggtgctgc cgtatgcggt cgacggctgc agcgtgacgg ccgactacgt gcggctcgat ttcgcgacgt ccgacgcgat cgaccgcgac gacgtcgagc ggcgcctcgc gggcttcgtc gtcggcgcgc atccggtcac gaccgaatgg atcaccgacg acgagatggc cgagcggccc gaactcgtgc gcacgatgag cgtgaagccg ccgatgggcc tcggccgcgt gcggctgctg cgcatcgaaa acgtcgacct gcagccgtgc ggcggcacgc atgtgcgcaa cacgtccgaa atcggtgggc tgcgggtcgc gaaactggaa aagaagagtg cgcgcacacg gcgcctcgta ctggagtttg catgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat