BuceA.00137.a

UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase

CENTER ID: BuceA.00137.a
ORGANISM: Burkholderia cenocepacia J2315
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 3
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
BuceA.00137.a.A2.GE33199 Mature protein( BuceA.00137.a ) 20 461
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|216591.5.peg.3545
RefSeq: YP_002232518.1
UniProt: B4E5U6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MHIHILGICG TFMGGLAVLA REAGHTVTGC DAGVYPPMST QLEAQGITLI EGYGAEQIDL KPDLFVIGNV VTRGNPLMEA ILDRGLPYVS GPQWLGEHVL AGKWVLAVAG THGKTTTSSM LAWLLEDAGL NPGFLIGGVP LNFGVSARLT DSSFFVIEAD EYDTAFFDKR SKFVHYRPRT AVLNNLEFDH ADIFPDLAAI ETQFHHLVRT VPGVGRIVTN GRSDALERVL ARGCWSEVER FGVDGGWQAL PAEDGVPVDE RFAVYSHAER VGEVAWQVQG DHNRMNALAA IAAARHVGVP PAQAAVSLAS FRNVKRRMEV RGSVDGVTVY DDFAHHPTAI DTTIAGLRAR IGRQNARILA VLEPRSNTMK LGVMKSQLPA SLADADLVFG YGAPTGRDAL GWNLAEALAP LGDRARAFDD LHLLVKAVVE AARPGDHVLV MSNGGFGGVH QKLLDALGSR T
NT Sequence
ATGCACATCC ATATTCTTGG CATCTGCGGC ACCTTCATGG GCGGTCTCGC CGTACTTGCA CGCGAGGCGG GCCACACGGT GACGGGTTGC GACGCGGGCG TCTATCCGCC GATGAGCACG CAGCTCGAGG CGCAGGGCAT CACGCTGATC GAGGGCTACG GCGCCGAGCA GATCGACCTG AAACCGGACC TGTTCGTGAT CGGCAACGTC GTCACGCGCG GCAACCCGCT GATGGAGGCG ATCCTCGATC GCGGCTTGCC GTACGTGTCG GGCCCGCAGT GGCTCGGCGA GCACGTGCTG GCCGGCAAAT GGGTGCTCGC GGTCGCGGGC ACGCACGGCA AGACGACCAC GTCGTCGATG CTCGCGTGGC TGCTGGAGGA CGCCGGGCTC AACCCGGGCT TCCTGATCGG CGGCGTGCCG CTGAACTTCG GCGTGTCCGC GCGGCTCACC GATTCGAGCT TCTTCGTGAT CGAGGCCGAC GAGTACGACA CGGCATTCTT CGACAAGCGC TCGAAATTCG TCCACTACCG GCCGCGTACC GCGGTACTGA ACAACCTCGA ATTCGATCAC GCCGACATCT TCCCCGATCT CGCCGCGATC GAGACGCAAT TCCATCATCT GGTGCGCACC GTGCCCGGGG TCGGCCGGAT CGTCACGAAC GGCCGCTCGG ATGCGCTCGA GCGCGTGCTG GCGCGCGGCT GCTGGAGCGA GGTCGAGCGT TTCGGCGTCG ACGGCGGCTG GCAGGCGCTG CCGGCCGAGG ACGGCGTGCC GGTCGACGAA CGCTTCGCGG TGTATTCGCA CGCCGAGCGC GTCGGCGAAG TCGCGTGGCA GGTGCAGGGC GACCACAACC GGATGAACGC GCTCGCGGCG ATCGCCGCCG CGCGCCACGT CGGCGTGCCG CCCGCGCAGG CGGCCGTTTC GCTCGCATCG TTCCGCAACG TGAAGCGCCG CATGGAAGTG CGCGGCAGCG TCGACGGCGT GACCGTCTAT GACGATTTCG CGCACCATCC GACCGCGATC GACACCACGA TCGCCGGCCT TCGTGCGCGC ATCGGCCGCC AGAATGCCCG CATCCTCGCC GTGCTCGAGC CGCGCTCGAA CACGATGAAG CTCGGCGTGA TGAAGTCGCA ATTGCCGGCG AGCCTCGCCG ATGCCGATCT CGTGTTCGGC TACGGCGCGC CGACCGGGCG CGATGCGCTC GGCTGGAATC TCGCCGAAGC GCTTGCGCCG CTCGGCGACC GCGCGCGCGC ATTCGACGAT CTGCATCTGC TGGTGAAGGC GGTGGTCGAG GCCGCGCGTC CGGGCGACCA CGTGCTCGTC ATGAGCAACG GCGGCTTCGG CGGCGTGCAC CAGAAGCTGC TCGATGCGCT CGGGAGCCGC ACG
Details for BuceA.00137.a.A2.GE33199
HARVESTED ON: 8/30/2011
SEQUENCED ON: 9/2/2011
EXPECTED MW: 49kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 97
PERCENT COVERAGE: 61
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMAREAGHTV TGCDAGVYPP MSTQLEAQGI TLIEGYGAEQ IDLKPDLFVI GNVVTRGNPL MEAILDRGLP YVSGPQWLGE HVLAGKWVLA VAGTHGKTTT SSMLAWLLED AGLNPGFLIG GVPLNFGVSA RLTDSSFFVI EADEYDTAFF DKRSKFVHYR PRTAVLNNLE FDHADIFPDL AAIETQFHHL VRTVPGVGRI VTNGRSDALE RVLARGCWSE VERFGVDXGW QALPAEDGVP VDERFAVYSH AERVGEVAWX XRATTTG
Validated NT Sequence
anntttgttt antttaagaa ggagatatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggca cgcgaggcgg gccacacggt gacgggttgc gacgcgggcg tctatccgcc gatgagcacg cagctcgagg cgcagggcat cacgctgatc gagggctacg gcgccgagca gatcgacctg aaaccggacc tgttcgtgat cggcaacgtc gtcacgcgcg gcaacccgct gatggaggcg atcctcgatc gcggcttgcc gtacgtgtcg ggcccgcagt ggctcggcga gcacgtgctg gccggcaaat gggtgctcgc ggtcgcgggc acgcacggca agacgaccac gtcgtcgatg ctcgcgtggc tgctggagga cgccgggctc aacccgggct tcctgatcgg cggcgtgccg ctgaacttcg gcgtgtccgc gcggctcacc gattcgagct tcttcgtgat cgaggccgac gagtacgaca cggcattctt cgacaagcgc tcgaaattcg tccactaccg gccgcgtacc gcggtactga acaacctcga attcgatcac gccgacatct tccccgatct cgccgcgatc gagacgcaat tccatcatct ggtgcgcacc gtgcccgggg tcggccggat cgtcacgaac ggccgctcgg atgcgctcga gcgcgtgctg gcgcgcggct gctggagcga ggtcgagcgt ttcggcgtcg acngcggctg gcaggcgctg ccggccgagg acggcgtgcc ggtcgacgaa cgcttcgcgg tgtattcgca cgccgagcgc gtcggcgaag tcgcgtggcn nngcagggcg accacaaccg gatgaacgcg ctcgcggcga tcgccgccgc gcgccacgtc ggcgtgccgc ccgcgcaggc ggccgtttcg ctcgcatcnt tccgcaacgt gaagcgccnc anggnagtgc gcggcagcgt cgannngtgn ccgtctatga cgatttcgcg cannntccna cngcgatcga cncncnatcn nngcntcnnn nngcatcngn nnnnannncn gcatcntcnn cnnnnnngan cnnnnannnn nannnnnnnn gnnnnnacna nnnnnannnn nnnnnnnngn nnnnnnnnnn nnnnngnnnn nnnnannnnn nnnnnaannn nnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMAREAGHTV TGCDAGVYPP MSTQLEAQGI TLIEGYGAEQ IDLKPDLFVI GNVVTRGNPL MEAILDRGLP YVSGPQWLGE HVLAGKWVLA VAGTHGKTTT SSMLAWLLED AGLNPGFLIG GVPLNFGVSA RLTDSSFFVI EADEYDTAFF DKRSKFVHYR PRTAVLNNLE FDHADIFPDL AAIETQFHHL VRTVPGVGRI VTNGRSDALE RVLARGCWSE VERFGVDGGW QALPAEDGVP VDERFAVYSH AERVGEVAWQ VQGDHNRMNA LAAIAAARHV GVPPAQAAVS LASFRNVKRR MEVRGSVDGV TVYDDFAHHP TAIDTTIAGL RARIGRQNAR ILAVLEPRSN TMKLGVMKSQ LPASLADADL VFGYGAPTGR DALGWNLAEA LAPLGDRARA FDDLHLLVKA VVEAARPGDH VLVMSNGGFG GVHQKLLDAL GSRT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcacgcgag gcgggccaca cggtgacggg ttgcgacgcg ggcgtctatc cgccgatgag cacgcagctc gaggcgcagg gcatcacgct gatcgagggc tacggcgccg agcagatcga cctgaaaccg gacctgttcg tgatcggcaa cgtcgtcacg cgcggcaacc cgctgatgga ggcgatcctc gatcgcggct tgccgtacgt gtcgggcccg cagtggctcg gcgagcacgt gctggccggc aaatgggtgc tcgcggtcgc gggcacgcac ggcaagacga ccacgtcgtc gatgctcgcg tggctgctgg aggacgccgg gctcaacccg ggcttcctga tcggcggcgt gccgctgaac ttcggcgtgt ccgcgcggct caccgattcg agcttcttcg tgatcgaggc cgacgagtac gacacggcat tcttcgacaa gcgctcgaaa ttcgtccact accggccgcg taccgcggta ctgaacaacc tcgaattcga tcacgccgac atcttccccg atctcgccgc gatcgagacg caattccatc atctggtgcg caccgtgccc ggggtcggcc ggatcgtcac gaacggccgc tcggatgcgc tcgagcgcgt gctggcgcgc ggctgctgga gcgaggtcga gcgtttcggc gtcgacggcg gctggcaggc gctgccggcc gaggacggcg tgccggtcga cgaacgcttc gcggtgtatt cgcacgccga gcgcgtcggc gaagtcgcgt ggcaggtgca gggcgaccac aaccggatga acgcgctcgc ggcgatcgcc gccgcgcgcc acgtcggcgt gccgcccgcg caggcggccg tttcgctcgc atcgttccgc aacgtgaagc gccgcatgga agtgcgcggc agcgtcgacg gcgtgaccgt ctatgacgat ttcgcgcacc atccgaccgc gatcgacacc acgatcgccg gccttcgtgc gcgcatcggc cgccagaatg cccgcatcct cgccgtgctc gagccgcgct cgaacacgat gaagctcggc gtgatgaagt cgcaattgcc ggcgagcctc gccgatgccg atctcgtgtt cggctacggc gcgccgaccg ggcgcgatgc gctcggctgg aatctcgccg aagcgcttgc gccgctcggc gaccgcgcgc gcgcattcga cgatctgcat ctgctggtga aggcggtggt cgaggccgcg cgtccgggcg accacgtgct cgtcatgagc aacggcggct tcggcggcgt gcaccagaag ctgctcgatg cgctcgggag ccgcacgtaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa